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The map label for this gene is gltA [H]

Identifier: 119774559

GI number: 119774559

Start: 1731406

End: 1732692

Strand: Reverse

Name: gltA [H]

Synonym: Sama_1422

Alternate gene names: 119774559

Gene position: 1732692-1731406 (Counterclockwise)

Preceding gene: 119774571

Following gene: 119774558

Centisome position: 40.24

GC content: 53.07

Gene sequence:

>1287_bases
ATGGCTGATTTGAAAGCCAAATTGGAATTACCGGGGAACGATTCAATTGAACTGCCGGTTAAACAGGGCACCGAAGGTTT
CGATGTTATCGATATCAGCAAGCTGGGCAGCAAAGGTTATTTCACCTTTGATCCAGGGTTTCTGGCCACAGCTTCCTGTG
AATCAGCAATTACCTATATCGACGGCGACAAGGGTATTTTGCTGCACCGTGGCTACCCCATCGAGCAGCTGGCAGTAGAA
TCCAACTATCTGGAACTCTGCTACGCCCTCCTGTACGGCGAACTGCCGACTAAAGCGCAATACGAACAATTTGCCCACAC
TGTGAAGAGCCACACCATGGTTCACGAGCAGTTGGCATTCTTCTTCCGCGGCTTCCGTCGCGATGCACACCCCATGGCCA
TGCTATGCGGTGTGACCGGTGCACTGTCTGCTTTCTACCAGGACTCTCTGGATGTAAACGACGCCCGTCACCGTGAAATT
GCCGCCTATCGTCTGGTATCCAAGATGCCGACGCTGGCAGCCATGTGCTACAAGTATTCCATCGGCCAGCCATTCGTGTA
CCCACGTAATGACTTGAGCTACGCCGGTAACTTCCTGTCGATGATGTTTGCCGTACCTTGCGAAGAGTACAAGGTAAACC
CAATCATCGAGCGCGCCATGGATCGCATCTTCATCCTGCACGCTGATCACGAACAGAATGCCTCTACTTCTACTGTGCGT
CTGGCCGGCTCTTCCGGTGCCAACCCATTCGCCTGTATCGCAGCCGGTATTGCGTCTCTGTGGGGTCCTGCCCACGGTGG
TGCCAACGAAGCCTGTCTGCGCATGCTGGAAGAAATCGGTACTGTTGACCGTATTCCTGAGTTTATCGACCGCGCCAAGG
ACAAGAATGATCCATTCCGTCTGATGGGCTTCGGTCACCGCGTGTACAAGAACTTTGACCCACGTGCCAAGGTAATGCGT
GAAACCTGCCACGAAGTGCTGAGCGAGCTCAAGGTGAACGACCCTCTGCTGGACGTTGCCATGGAGCTCGAGCGTATTGC
CCTCAATGATGAGTACTTTATCTCCAAGAAGCTCTACCCGAACGTAGACTTCTACTCAGGTATCATCATGAAGGCCATTG
GCATTCCAACCAGCATGTTCACCGTGATTTTTGCTCTGGCCCGCACCGTTGGCTGGATCTCTCACTGGAAAGAAATGCTG
GATCAGCCTGGTCACAAGATCAGTCGCCCACGTCAGCTGTACACCGGCGAACACAGCCGCGATTTTGTCAGTGTCGATAA
GCGATAA

Upstream 100 bases:

>100_bases
AACGCTTATTTAAATTGAAATGTGATCTCGATTGCTTGTAAAGTAGGGGCCGTTTTGACATGCCGCACTCACCACAAGAA
TGAAGTAAGGAGAACGGGGT

Downstream 100 bases:

>100_bases
CGACTAATCCTGTTAAAAAGGCGCCTCTGGCGCCTTTTTTGTTTCTGTTTCGCCTTTCCAGCTTCATCTTTTGTTGCAAT
TCCCGCCTTGAAGTTCACTT

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MADLKAKLELPGNDSIELPVKQGTEGFDVIDISKLGSKGYFTFDPGFLATASCESAITYIDGDKGILLHRGYPIEQLAVE
SNYLELCYALLYGELPTKAQYEQFAHTVKSHTMVHEQLAFFFRGFRRDAHPMAMLCGVTGALSAFYQDSLDVNDARHREI
AAYRLVSKMPTLAAMCYKYSIGQPFVYPRNDLSYAGNFLSMMFAVPCEEYKVNPIIERAMDRIFILHADHEQNASTSTVR
LAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGTVDRIPEFIDRAKDKNDPFRLMGFGHRVYKNFDPRAKVMR
ETCHEVLSELKVNDPLLDVAMELERIALNDEYFISKKLYPNVDFYSGIIMKAIGIPTSMFTVIFALARTVGWISHWKEML
DQPGHKISRPRQLYTGEHSRDFVSVDKR

Sequences:

>Translated_428_residues
MADLKAKLELPGNDSIELPVKQGTEGFDVIDISKLGSKGYFTFDPGFLATASCESAITYIDGDKGILLHRGYPIEQLAVE
SNYLELCYALLYGELPTKAQYEQFAHTVKSHTMVHEQLAFFFRGFRRDAHPMAMLCGVTGALSAFYQDSLDVNDARHREI
AAYRLVSKMPTLAAMCYKYSIGQPFVYPRNDLSYAGNFLSMMFAVPCEEYKVNPIIERAMDRIFILHADHEQNASTSTVR
LAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGTVDRIPEFIDRAKDKNDPFRLMGFGHRVYKNFDPRAKVMR
ETCHEVLSELKVNDPLLDVAMELERIALNDEYFISKKLYPNVDFYSGIIMKAIGIPTSMFTVIFALARTVGWISHWKEML
DQPGHKISRPRQLYTGEHSRDFVSVDKR
>Mature_427_residues
ADLKAKLELPGNDSIELPVKQGTEGFDVIDISKLGSKGYFTFDPGFLATASCESAITYIDGDKGILLHRGYPIEQLAVES
NYLELCYALLYGELPTKAQYEQFAHTVKSHTMVHEQLAFFFRGFRRDAHPMAMLCGVTGALSAFYQDSLDVNDARHREIA
AYRLVSKMPTLAAMCYKYSIGQPFVYPRNDLSYAGNFLSMMFAVPCEEYKVNPIIERAMDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGTVDRIPEFIDRAKDKNDPFRLMGFGHRVYKNFDPRAKVMRE
TCHEVLSELKVNDPLLDVAMELERIALNDEYFISKKLYPNVDFYSGIIMKAIGIPTSMFTVIFALARTVGWISHWKEMLD
QPGHKISRPRQLYTGEHSRDFVSVDKR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=364, Percent_Identity=25.5494505494505, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786939, Length=428, Percent_Identity=76.6355140186916, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=370, Percent_Identity=30.8108108108108, Blast_Score=158, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=360, Percent_Identity=28.0555555555556, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=376, Percent_Identity=26.3297872340426, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=377, Percent_Identity=25.9946949602122, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=377, Percent_Identity=26.790450928382, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640126, Length=352, Percent_Identity=27.2727272727273, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640124, Length=352, Percent_Identity=27.2727272727273, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356863, Length=401, Percent_Identity=25.4364089775561, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48135; Mature: 48004

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLKAKLELPGNDSIELPVKQGTEGFDVIDISKLGSKGYFTFDPGFLATASCESAITYI
CCCCCEEEECCCCCCEEECCCCCCCCCCEEEHHHCCCCCEEEECCCCEEECCCCCCEEEE
DGDKGILLHRGYPIEQLAVESNYLELCYALLYGELPTKAQYEQFAHTVKSHTMVHEQLAF
ECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FFRGFRRDAHPMAMLCGVTGALSAFYQDSLDVNDARHREIAAYRLVSKMPTLAAMCYKYS
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
IGQPFVYPRNDLSYAGNFLSMMFAVPCEEYKVNPIIERAMDRIFILHADHEQNASTSTVR
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHEEEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGTVDRIPEFIDRAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
LMGFGHRVYKNFDPRAKVMRETCHEVLSELKVNDPLLDVAMELERIALNDEYFISKKLYP
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCC
NVDFYSGIIMKAIGIPTSMFTVIFALARTVGWISHWKEMLDQPGHKISRPRQLYTGEHSR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
DFVSVDKR
CCCCCCCC
>Mature Secondary Structure 
ADLKAKLELPGNDSIELPVKQGTEGFDVIDISKLGSKGYFTFDPGFLATASCESAITYI
CCCCEEEECCCCCCEEECCCCCCCCCCEEEHHHCCCCCEEEECCCCEEECCCCCCEEEE
DGDKGILLHRGYPIEQLAVESNYLELCYALLYGELPTKAQYEQFAHTVKSHTMVHEQLAF
ECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FFRGFRRDAHPMAMLCGVTGALSAFYQDSLDVNDARHREIAAYRLVSKMPTLAAMCYKYS
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
IGQPFVYPRNDLSYAGNFLSMMFAVPCEEYKVNPIIERAMDRIFILHADHEQNASTSTVR
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHEEEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGTVDRIPEFIDRAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
LMGFGHRVYKNFDPRAKVMRETCHEVLSELKVNDPLLDVAMELERIALNDEYFISKKLYP
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCC
NVDFYSGIIMKAIGIPTSMFTVIFALARTVGWISHWKEMLDQPGHKISRPRQLYTGEHSR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
DFVSVDKR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]