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The map label for this gene is tig

Identifier: 119774360

GI number: 119774360

Start: 1504977

End: 1506281

Strand: Direct

Name: tig

Synonym: Sama_1223

Alternate gene names: 119774360

Gene position: 1504977-1506281 (Clockwise)

Preceding gene: 119774358

Following gene: 119774361

Centisome position: 34.95

GC content: 53.1

Gene sequence:

>1305_bases
ATGCAAGTTTCTGTTGAAACAACTCAAGGCTTAGAGCGTCGCCTGACCATCTCTGTACCTGCCGAGCAGATCGAAAAACT
GGTTAACGAGAGCCTGAAGCGCGAAGCCAAGCGTGCCCGTATCCCTGGTTTCCGTCCTGGTAAAGTACCTATGAGCGTTG
TCAGCAAGCGCTACGGCGCCGCTATCCGTCAGGACGTGACTGGCGAAATCATGCAGCGCAATTTTGTTGAAGCCATCATC
GCCGAGAAGCTGAATCCAGCTGGCGCACCTACTTTCATGCCAGGTGCAAGCGATGCCGACAAGTTTGAATTTGTTGCCAC
TTTTGAAGTGTATCCAGAAGTAGAACTCAAAGGTCTGGATGCTATCGAAGTTGAACAGCCAAAAGCTGATGTGACCGATG
CTGACGTAGACAGCATGATCGAAACCCTGCGCAAGCAACATGCTACCTTCGACGCCGCCCCGCGCGCTGCTGAAGAAGGC
GACAAAGTCGTTATCAACTTTGTTGGCAGCGTAGACGGAGAAGAGTTCCAGGGTGGCAAGGCCGAAGGCTTTGAGCTGCA
ACTGGGCAGCGGTCGTATGATCCCTGGCTTCGAAGAAGGCATCAAAGGCCACAAGGCCGGTGAAGAATTCGAAATCGACG
TGACCTTCCCTGAGGAATACCACGCTGAAAACCTGAAGGGCAAAGCGGCCAAGTTCGCTATCACCCTGACTGAGGTGAAA
GCGGCTAACCTGCCAGAAGTAAACGACGAGTTTGCCAAGCTGTTCGGTATCACCGATGGCGGCGTTGAGGCTCTAAAGGC
CGAAATCCGCAAGAACATGAGCCGTGAGCTGGAACAGGCCCTGAAAGCCAACGTGAAAGAGCAGGTGATCAACGGTCTGC
TGGCCGCCAACGACATCGAGCTGCCAAAGGCCCTGATCGACGGCGAAATCAACGTACTGCGTCGTCAAGCTGTTCAGCGT
TTCGGTGGCAATGCTGCCAACATGCCTGAGCTGCCAGCCGAGTTGTTTGCTGAGCAAGCCGAGCGCCGTGTAAAAGTGGG
CCTGCTGCTGGGTGAAGTTATCAAATCTACCGAGCTGAAAGTAGAAGAAGAACGCGTTCAGGCGCTGATCGCTTCTATGG
CTTCTGCCTACGAAGATCCAGCCGAAGTGATTGAATACTACAACGGCAACAAAGAGCTGATGCAGAACATGCGCAACGTG
GCTCTGGAAGAGCAAGCCGTTGAAGCTCTGCTGAAATCTGCAAAAGTGACCGAGAAGGAAGTTGCTTTTGAAGAATTTAT
GAACAAGGCTACCGGCCGCGCTTAA

Upstream 100 bases:

>100_bases
CAGGCATTAAAGTCAATTTCAAAGATCAAGAAACGAACACCTTAATAGTTGAACACTCGGCTATTTACAAAGGACGTTAG
ACATATTTCGAGGTAAAACA

Downstream 100 bases:

>100_bases
CCGCAGCTTGACTTGATTGGCGTTAAGCCATTTATAATGGCTCGTATGAGGCTCCTCATGCGGGCCATTTTTATTTAGGG
AAGGGATAAATGCATAACGC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MQVSVETTQGLERRLTISVPAEQIEKLVNESLKREAKRARIPGFRPGKVPMSVVSKRYGAAIRQDVTGEIMQRNFVEAII
AEKLNPAGAPTFMPGASDADKFEFVATFEVYPEVELKGLDAIEVEQPKADVTDADVDSMIETLRKQHATFDAAPRAAEEG
DKVVINFVGSVDGEEFQGGKAEGFELQLGSGRMIPGFEEGIKGHKAGEEFEIDVTFPEEYHAENLKGKAAKFAITLTEVK
AANLPEVNDEFAKLFGITDGGVEALKAEIRKNMSRELEQALKANVKEQVINGLLAANDIELPKALIDGEINVLRRQAVQR
FGGNAANMPELPAELFAEQAERRVKVGLLLGEVIKSTELKVEEERVQALIASMASAYEDPAEVIEYYNGNKELMQNMRNV
ALEEQAVEALLKSAKVTEKEVAFEEFMNKATGRA

Sequences:

>Translated_434_residues
MQVSVETTQGLERRLTISVPAEQIEKLVNESLKREAKRARIPGFRPGKVPMSVVSKRYGAAIRQDVTGEIMQRNFVEAII
AEKLNPAGAPTFMPGASDADKFEFVATFEVYPEVELKGLDAIEVEQPKADVTDADVDSMIETLRKQHATFDAAPRAAEEG
DKVVINFVGSVDGEEFQGGKAEGFELQLGSGRMIPGFEEGIKGHKAGEEFEIDVTFPEEYHAENLKGKAAKFAITLTEVK
AANLPEVNDEFAKLFGITDGGVEALKAEIRKNMSRELEQALKANVKEQVINGLLAANDIELPKALIDGEINVLRRQAVQR
FGGNAANMPELPAELFAEQAERRVKVGLLLGEVIKSTELKVEEERVQALIASMASAYEDPAEVIEYYNGNKELMQNMRNV
ALEEQAVEALLKSAKVTEKEVAFEEFMNKATGRA
>Mature_434_residues
MQVSVETTQGLERRLTISVPAEQIEKLVNESLKREAKRARIPGFRPGKVPMSVVSKRYGAAIRQDVTGEIMQRNFVEAII
AEKLNPAGAPTFMPGASDADKFEFVATFEVYPEVELKGLDAIEVEQPKADVTDADVDSMIETLRKQHATFDAAPRAAEEG
DKVVINFVGSVDGEEFQGGKAEGFELQLGSGRMIPGFEEGIKGHKAGEEFEIDVTFPEEYHAENLKGKAAKFAITLTEVK
AANLPEVNDEFAKLFGITDGGVEALKAEIRKNMSRELEQALKANVKEQVINGLLAANDIELPKALIDGEINVLRRQAVQR
FGGNAANMPELPAELFAEQAERRVKVGLLLGEVIKSTELKVEEERVQALIASMASAYEDPAEVIEYYNGNKELMQNMRNV
ALEEQAVEALLKSAKVTEKEVAFEEFMNKATGRA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=430, Percent_Identity=66.046511627907, Blast_Score=569, Evalue=1e-164,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_SHEAM (A1S4X4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927100.1
- ProteinModelPortal:   A1S4X4
- SMR:   A1S4X4
- STRING:   A1S4X4
- GeneID:   4605949
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1223
- NMPDR:   fig|326297.7.peg.1154
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   A1S4X4
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47671; Mature: 47671

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSVETTQGLERRLTISVPAEQIEKLVNESLKREAKRARIPGFRPGKVPMSVVSKRYGA
CEEECCHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AIRQDVTGEIMQRNFVEAIIAEKLNPAGAPTFMPGASDADKFEFVATFEVYPEVELKGLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEECCCC
AIEVEQPKADVTDADVDSMIETLRKQHATFDAAPRAAEEGDKVVINFVGSVDGEEFQGGK
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
AEGFELQLGSGRMIPGFEEGIKGHKAGEEFEIDVTFPEEYHAENLKGKAAKFAITLTEVK
CCCEEEEECCCCCCCCHHHHCCCCCCCCEEEEEEECCHHHHHHCCCCCHHEEEEEEEHHC
AANLPEVNDEFAKLFGITDGGVEALKAEIRKNMSRELEQALKANVKEQVINGLLAANDIE
CCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LPKALIDGEINVLRRQAVQRFGGNAANMPELPAELFAEQAERRVKVGLLLGEVIKSTELK
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEEERVQALIASMASAYEDPAEVIEYYNGNKELMQNMRNVALEEQAVEALLKSAKVTEKE
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VAFEEFMNKATGRA
HHHHHHHHHHCCCC
>Mature Secondary Structure
MQVSVETTQGLERRLTISVPAEQIEKLVNESLKREAKRARIPGFRPGKVPMSVVSKRYGA
CEEECCHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AIRQDVTGEIMQRNFVEAIIAEKLNPAGAPTFMPGASDADKFEFVATFEVYPEVELKGLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEECCCC
AIEVEQPKADVTDADVDSMIETLRKQHATFDAAPRAAEEGDKVVINFVGSVDGEEFQGGK
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
AEGFELQLGSGRMIPGFEEGIKGHKAGEEFEIDVTFPEEYHAENLKGKAAKFAITLTEVK
CCCEEEEECCCCCCCCHHHHCCCCCCCCEEEEEEECCHHHHHHCCCCCHHEEEEEEEHHC
AANLPEVNDEFAKLFGITDGGVEALKAEIRKNMSRELEQALKANVKEQVINGLLAANDIE
CCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LPKALIDGEINVLRRQAVQRFGGNAANMPELPAELFAEQAERRVKVGLLLGEVIKSTELK
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEEERVQALIASMASAYEDPAEVIEYYNGNKELMQNMRNVALEEQAVEALLKSAKVTEKE
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VAFEEFMNKATGRA
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA