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The map label for this gene is cysS

Identifier: 119774358

GI number: 119774358

Start: 1501835

End: 1503214

Strand: Direct

Name: cysS

Synonym: Sama_1221

Alternate gene names: 119774358

Gene position: 1501835-1503214 (Clockwise)

Preceding gene: 119774355

Following gene: 119774360

Centisome position: 34.88

GC content: 53.19

Gene sequence:

>1380_bases
ATGTTGAAGATATACAATAGTATCAGTCGTCAAAAAGAGGAGTTTAAACCCATACAGCCAGGCAAAATTGGCATGTATGT
TTGTGGTGTGACGATCTATGACCTGTGCCACATCGGCCATGGTCGTACTTTTGTGTGTTTTGACATGATTGTGCGTTATC
TGCGTTACCGCGGTTACGAGGTAAATTACCAGCGCAATATCACCGACGTGGATGACAAGATCATCAAGCGCGCCAACGAA
AATGGCGAGAGCTGTGACAGCCTCACTGAGCGTTTAATTGGTGAAATGCATGCCGATTTCGACGCGCTAAACATGGTGCG
TCCAAGCTTCGAACCCCGCGCAACCCTTCACATCGACGAAATCATCGACATGGTGACCCGTTTGCTCGACCGTGGTCATG
CGTATGTCGCTGCCGATGGCGACGTGCTGTTTGATGTGTCCTCGTATGCCGCATACGGCCGCCTGTCAGGGCAGGACCTC
GAGCAGCTGCAAGCCGGTGCTCGCGTTGAAGTGGATGAAAACAAACGTAATCCAATGGATTTCGTGCTGTGGAAGATGTC
CAAACCCGGCGAGCCTACCTGGGATTCGCCGTGGGGTCCGGGTCGTCCAGGTTGGCACATCGAGTGCTCGGCCATGAACA
GCAAACATCTGGGGCTTCATTTCGACATCCATGGCGGTGGCAGTGATTTGCAGTTCCCGCACCATGAAAACGAAATTGCC
CAGTCCTGCTGCGCCCATGACACACCATATGTGAACACCTGGATGCACACAGGTATGGTGATGGTTGACCGCGAGAAGAT
GTCCAAGTCCCTTGGCAACTTCTTCACCATCCGTGATGTGCTTGCACATTACGATGCCCAAACCGTGCGCTATTTCCTGC
TTTCCGGCCACTATCGCAGCCAGCTGAATTACAGCGAAGACAATTTGAAACAGGCTCGTGCTTCTCTCGAGCGTATGTAT
ACCGCACTCAAAGGTCTGGATCTGACCGTCGAAGCCGCTGGTGCTGATGAGTTTGTCAGCCGCTTTATGGCCGCAATGGA
CGATGACTTCAACACCCCAGAAGCCTATTCAGTGCTCTTTGACATGGTGCGTGAAATTAACCGTCTGAAAACCAGCGACA
TGGCCGCAGCATCCCAATTGGGTGTGGCACTCAAAGAGCTTGCCGAAGTACTGGGCATTTTGTCTTCAACACCTGAGGCT
TTTCTCCAGGGCGAAGGTAATGCCGACGAAGTGGCTGAAATTGAAGCACTGATCGTTGAGCGCAACCGTGCCCGCGCCGA
GAAGGACTGGCCTGCTGCCGACGTGGCCCGTGACCGCTTGAACGCCCTCGGCGTAGTGCTTGAAGATGGCCCGGAAGGGA
CTACCTGGCGAAAGAAGTAA

Upstream 100 bases:

>100_bases
ATCTGGAACGGCCCTGAAAGTGCAGTATTCAAGGGCTTTGGAGCATGATAGACTCACAGGCCTGATTTTACACCCAGTGC
TTAGCGGAAAGAGAGCAAGA

Downstream 100 bases:

>100_bases
ACTGCTTCTGAGTACCAATAAAAACGCCTGCAATTGCAGGCGTTTTTATTGGTACATCGTTATGTTGCTGCCGTTACGCT
GGCAACTTTTGCCATAGTAA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLKIYNSISRQKEEFKPIQPGKIGMYVCGVTIYDLCHIGHGRTFVCFDMIVRYLRYRGYEVNYQRNITDVDDKIIKRANE
NGESCDSLTERLIGEMHADFDALNMVRPSFEPRATLHIDEIIDMVTRLLDRGHAYVAADGDVLFDVSSYAAYGRLSGQDL
EQLQAGARVEVDENKRNPMDFVLWKMSKPGEPTWDSPWGPGRPGWHIECSAMNSKHLGLHFDIHGGGSDLQFPHHENEIA
QSCCAHDTPYVNTWMHTGMVMVDREKMSKSLGNFFTIRDVLAHYDAQTVRYFLLSGHYRSQLNYSEDNLKQARASLERMY
TALKGLDLTVEAAGADEFVSRFMAAMDDDFNTPEAYSVLFDMVREINRLKTSDMAAASQLGVALKELAEVLGILSSTPEA
FLQGEGNADEVAEIEALIVERNRARAEKDWPAADVARDRLNALGVVLEDGPEGTTWRKK

Sequences:

>Translated_459_residues
MLKIYNSISRQKEEFKPIQPGKIGMYVCGVTIYDLCHIGHGRTFVCFDMIVRYLRYRGYEVNYQRNITDVDDKIIKRANE
NGESCDSLTERLIGEMHADFDALNMVRPSFEPRATLHIDEIIDMVTRLLDRGHAYVAADGDVLFDVSSYAAYGRLSGQDL
EQLQAGARVEVDENKRNPMDFVLWKMSKPGEPTWDSPWGPGRPGWHIECSAMNSKHLGLHFDIHGGGSDLQFPHHENEIA
QSCCAHDTPYVNTWMHTGMVMVDREKMSKSLGNFFTIRDVLAHYDAQTVRYFLLSGHYRSQLNYSEDNLKQARASLERMY
TALKGLDLTVEAAGADEFVSRFMAAMDDDFNTPEAYSVLFDMVREINRLKTSDMAAASQLGVALKELAEVLGILSSTPEA
FLQGEGNADEVAEIEALIVERNRARAEKDWPAADVARDRLNALGVVLEDGPEGTTWRKK
>Mature_459_residues
MLKIYNSISRQKEEFKPIQPGKIGMYVCGVTIYDLCHIGHGRTFVCFDMIVRYLRYRGYEVNYQRNITDVDDKIIKRANE
NGESCDSLTERLIGEMHADFDALNMVRPSFEPRATLHIDEIIDMVTRLLDRGHAYVAADGDVLFDVSSYAAYGRLSGQDL
EQLQAGARVEVDENKRNPMDFVLWKMSKPGEPTWDSPWGPGRPGWHIECSAMNSKHLGLHFDIHGGGSDLQFPHHENEIA
QSCCAHDTPYVNTWMHTGMVMVDREKMSKSLGNFFTIRDVLAHYDAQTVRYFLLSGHYRSQLNYSEDNLKQARASLERMY
TALKGLDLTVEAAGADEFVSRFMAAMDDDFNTPEAYSVLFDMVREINRLKTSDMAAASQLGVALKELAEVLGILSSTPEA
FLQGEGNADEVAEIEALIVERNRARAEKDWPAADVARDRLNALGVVLEDGPEGTTWRKK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=428, Percent_Identity=36.9158878504673, Blast_Score=242, Evalue=5e-64,
Organism=Homo sapiens, GI10835051, Length=233, Percent_Identity=42.9184549356223, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI62240992, Length=233, Percent_Identity=42.9184549356223, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI21269877, Length=233, Percent_Identity=42.9184549356223, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI303304987, Length=233, Percent_Identity=42.9184549356223, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI1786737, Length=461, Percent_Identity=72.0173535791757, Blast_Score=690, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17509797, Length=246, Percent_Identity=40.2439024390244, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17509795, Length=246, Percent_Identity=40.2439024390244, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324082, Length=313, Percent_Identity=34.8242811501597, Blast_Score=192, Evalue=8e-50,
Organism=Drosophila melanogaster, GI19922218, Length=386, Percent_Identity=40.9326424870466, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI19922364, Length=232, Percent_Identity=41.8103448275862, Blast_Score=181, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_SHEAM (A1S4X2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927098.1
- ProteinModelPortal:   A1S4X2
- SMR:   A1S4X2
- STRING:   A1S4X2
- GeneID:   4603473
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1221
- NMPDR:   fig|326297.7.peg.1152
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   A1S4X2
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51839; Mature: 51839

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIYNSISRQKEEFKPIQPGKIGMYVCGVTIYDLCHIGHGRTFVCFDMIVRYLRYRGYE
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCE
VNYQRNITDVDDKIIKRANENGESCDSLTERLIGEMHADFDALNMVRPSFEPRATLHIDE
EEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEHHH
IIDMVTRLLDRGHAYVAADGDVLFDVSSYAAYGRLSGQDLEQLQAGARVEVDENKRNPMD
HHHHHHHHHHCCCEEEEECCCEEEEEHHHHHHCCCCCCCHHHHHCCCEEEECCCCCCCCE
FVLWKMSKPGEPTWDSPWGPGRPGWHIECSAMNSKHLGLHFDIHGGGSDLQFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHH
QSCCAHDTPYVNTWMHTGMVMVDREKMSKSLGNFFTIRDVLAHYDAQTVRYFLLSGHYRS
HHHHCCCCCCHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCHHHH
QLNYSEDNLKQARASLERMYTALKGLDLTVEAAGADEFVSRFMAAMDDDFNTPEAYSVLF
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHH
DMVREINRLKTSDMAAASQLGVALKELAEVLGILSSTPEAFLQGEGNADEVAEIEALIVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
RNRARAEKDWPAADVARDRLNALGVVLEDGPEGTTWRKK
HHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKIYNSISRQKEEFKPIQPGKIGMYVCGVTIYDLCHIGHGRTFVCFDMIVRYLRYRGYE
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCE
VNYQRNITDVDDKIIKRANENGESCDSLTERLIGEMHADFDALNMVRPSFEPRATLHIDE
EEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEHHH
IIDMVTRLLDRGHAYVAADGDVLFDVSSYAAYGRLSGQDLEQLQAGARVEVDENKRNPMD
HHHHHHHHHHCCCEEEEECCCEEEEEHHHHHHCCCCCCCHHHHHCCCEEEECCCCCCCCE
FVLWKMSKPGEPTWDSPWGPGRPGWHIECSAMNSKHLGLHFDIHGGGSDLQFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHH
QSCCAHDTPYVNTWMHTGMVMVDREKMSKSLGNFFTIRDVLAHYDAQTVRYFLLSGHYRS
HHHHCCCCCCHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCHHHH
QLNYSEDNLKQARASLERMYTALKGLDLTVEAAGADEFVSRFMAAMDDDFNTPEAYSVLF
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHH
DMVREINRLKTSDMAAASQLGVALKELAEVLGILSSTPEAFLQGEGNADEVAEIEALIVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
RNRARAEKDWPAADVARDRLNALGVVLEDGPEGTTWRKK
HHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA