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The map label for this gene is puuD [H]

Identifier: 119773931

GI number: 119773931

Start: 978676

End: 979479

Strand: Direct

Name: puuD [H]

Synonym: Sama_0794

Alternate gene names: 119773931

Gene position: 978676-979479 (Clockwise)

Preceding gene: 119773930

Following gene: 119773933

Centisome position: 22.73

GC content: 54.35

Gene sequence:

>804_bases
ATGCCCTTACAACAAGAATCAGCAAGACAAGAACGAAAACCGGTCATTTTAATGTCCATGGGTCAGCAAGACAGAAATGG
CCACGCCTATCAGGTCATGACCCACAAATATATGCAGCCAGTGGTAGACATTTCCGACTGCATTCCGCTACTTATCCCTA
CCTGCTTCGGTGTGGCAGATATTGAACAATATCTGGACATGGCCGACGGCGTATACCTTTCCGGTGCCGCCTCCAATATC
GACCCCAGTCTCTATGGTCAGGAAAACCTCACGCCCGAAAAAAAGCAGGATTTGGCACGGGATCTGGTGGATATTGCGCT
TATCAAGGGTGCCGTTAAACGGGGCTTGCCGATACTGGGCATCTGCCGTGGCATGCAGGAGATGAATATCGCCTTTGGCG
GCGATCTTTATCAGAAGGTGCATGATGAGGACCATCTGAACGATCACCGTGAAGACCCGGACACACCGCCCGACGTGCAG
TATGGCGCCAGTCACAGCATCAGCATGGTGAAAGGCTCCTGGCTGCATAAGCTGCTCGGGGACACCATAGAGGTTAACTC
CCTCCACGGTCAGGGCATTAAAACCCTGGGTAAGGGGTTGGAAGCGCTGGCGTTGGCCGAAGATGGTCTGGTGGAGGCGC
TGCACGCTCCTTATCTGCCGCAGTTCACCCTTGGGGTACAGTGGCATCCCGAGTGGAAGGCGCTTGAAAACCCTGACTCC
ATCAAGATTTTCAAGGCCTTTGGTGAGGCTTGCCGTCGCCGTGCTGGCAGCGCCCTGGATCTGAGAATCGACAAGGCGTC
CTGA

Upstream 100 bases:

>100_bases
GCCGTTTAAACCAACGCATCATCCTGATAAGCGTATGGTTTTAACCATGCCAGGCTATGCCCAAAGCGCGAACTCAACGC
GTTTGAAAATCAGGTGATCT

Downstream 100 bases:

>100_bases
AAATAACGGATTACGGGCAACAAAAAAGCGAACCCGGGGCTTCGCTTTTTTTATGTGCTTAACACAGCTGTTTGGCTTAT
TCGCCCTGTTCTACACCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MPLQQESARQERKPVILMSMGQQDRNGHAYQVMTHKYMQPVVDISDCIPLLIPTCFGVADIEQYLDMADGVYLSGAASNI
DPSLYGQENLTPEKKQDLARDLVDIALIKGAVKRGLPILGICRGMQEMNIAFGGDLYQKVHDEDHLNDHREDPDTPPDVQ
YGASHSISMVKGSWLHKLLGDTIEVNSLHGQGIKTLGKGLEALALAEDGLVEALHAPYLPQFTLGVQWHPEWKALENPDS
IKIFKAFGEACRRRAGSALDLRIDKAS

Sequences:

>Translated_267_residues
MPLQQESARQERKPVILMSMGQQDRNGHAYQVMTHKYMQPVVDISDCIPLLIPTCFGVADIEQYLDMADGVYLSGAASNI
DPSLYGQENLTPEKKQDLARDLVDIALIKGAVKRGLPILGICRGMQEMNIAFGGDLYQKVHDEDHLNDHREDPDTPPDVQ
YGASHSISMVKGSWLHKLLGDTIEVNSLHGQGIKTLGKGLEALALAEDGLVEALHAPYLPQFTLGVQWHPEWKALENPDS
IKIFKAFGEACRRRAGSALDLRIDKAS
>Mature_266_residues
PLQQESARQERKPVILMSMGQQDRNGHAYQVMTHKYMQPVVDISDCIPLLIPTCFGVADIEQYLDMADGVYLSGAASNID
PSLYGQENLTPEKKQDLARDLVDIALIKGAVKRGLPILGICRGMQEMNIAFGGDLYQKVHDEDHLNDHREDPDTPPDVQY
GASHSISMVKGSWLHKLLGDTIEVNSLHGQGIKTLGKGLEALALAEDGLVEALHAPYLPQFTLGVQWHPEWKALENPDSI
KIFKAFGEACRRRAGSALDLRIDKAS

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=243, Percent_Identity=39.917695473251, Blast_Score=174, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 29507; Mature: 29376

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLQQESARQERKPVILMSMGQQDRNGHAYQVMTHKYMQPVVDISDCIPLLIPTCFGVAD
CCCCHHHHHHHHCCEEEEECCCCCCCCCEEEHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
IEQYLDMADGVYLSGAASNIDPSLYGQENLTPEKKQDLARDLVDIALIKGAVKRGLPILG
HHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ICRGMQEMNIAFGGDLYQKVHDEDHLNDHREDPDTPPDVQYGASHSISMVKGSWLHKLLG
HHCCHHHHHHHHCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
DTIEVNSLHGQGIKTLGKGLEALALAEDGLVEALHAPYLPQFTLGVQWHPEWKALENPDS
CCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCEECCCCCCCCCCCH
IKIFKAFGEACRRRAGSALDLRIDKAS
HHHHHHHHHHHHHHCCCEEEEEEECCC
>Mature Secondary Structure 
PLQQESARQERKPVILMSMGQQDRNGHAYQVMTHKYMQPVVDISDCIPLLIPTCFGVAD
CCCHHHHHHHHCCEEEEECCCCCCCCCEEEHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
IEQYLDMADGVYLSGAASNIDPSLYGQENLTPEKKQDLARDLVDIALIKGAVKRGLPILG
HHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ICRGMQEMNIAFGGDLYQKVHDEDHLNDHREDPDTPPDVQYGASHSISMVKGSWLHKLLG
HHCCHHHHHHHHCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
DTIEVNSLHGQGIKTLGKGLEALALAEDGLVEALHAPYLPQFTLGVQWHPEWKALENPDS
CCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCEECCCCCCCCCCCH
IKIFKAFGEACRRRAGSALDLRIDKAS
HHHHHHHHHHHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]