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The map label for this gene is trmH [H]

Identifier: 119773403

GI number: 119773403

Start: 301630

End: 302355

Strand: Reverse

Name: trmH [H]

Synonym: Sama_0262

Alternate gene names: 119773403

Gene position: 302355-301630 (Counterclockwise)

Preceding gene: 119773404

Following gene: 119773402

Centisome position: 7.02

GC content: 58.4

Gene sequence:

>726_bases
ATGAGCCCGGAACGCTTTGCCCGCATCAATGAGATGCTGGACAACCGTCAACCGGATCTGACCGTCTTACTCGACCAGGT
CCACAAGACCAACAACATTGCAGCAGTGCTGCGCAGTGCCGATGCCGTGGGTGTCCACCAGCTGCATGCGGTGTGGCCAG
AAAGCGCCATGCGGGTATCCGGCAATACCGCCTCGGGCAGCCAGCAATGGGTTAAAACCCTGCGCCATCAAACGGTGACC
GATGCCATCACCGCCCTCAAAGCAGATGGTATGCAGGTGGTGGTGACCAATTTCTCCCCCAATGCCAAAGACTTTCGCGA
GATTGATTACACTAAACCCACCGCCATCGTACTCGGGCACGAGAAAAACGGTGTCAGTGATGAAGCCGTGCGCCTTGCCG
ATGCCGAAGTGATTATTCCCATGGTGGGCATGGTGCAATCGTTGAATGTGTCAGTGGCCGGTGCCCTGGTACTGTTTGAG
GCCGAGCGCCAGCGCCGGGCTACAGGCATGTATGGCAACCGAAAACTTCCCGAAGACTATTGTCAGAAGCTGCTGTTCGA
GCAAGGCCATCCCATCTATGCCAAGGCCTGTCGTCGCAAGGGACTGGTCTACCCCGCTATTGATGAAACCGGCCAAATCA
TCGCGCCGCCCGATTGGTGGCAAAAGATGCGCGAAGCCGACCCCGACTCCCCGCGTCCCGAACGCGGCCGCCAGCGCCGC
ATGTAA

Upstream 100 bases:

>100_bases
AGGTAGAAATGGATGGGGTGATGGAGCTGTAATTGCTTCCACATCTTCAGGGCGCCTCGGCGCCCTTTTGTTTTTTCACG
ATTTACACAGGAATGATTCC

Downstream 100 bases:

>100_bases
TTCAGCTTTGCATCAGGCTGAAGCGCCTATAATCCGGGAGAATCAGGCTGTTGTCCGCGTTTTTGTGTGGTGATGGCCGC
AGCCTGTGGGCTTCTGGCCA

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSPERFARINEMLDNRQPDLTVLLDQVHKTNNIAAVLRSADAVGVHQLHAVWPESAMRVSGNTASGSQQWVKTLRHQTVT
DAITALKADGMQVVVTNFSPNAKDFREIDYTKPTAIVLGHEKNGVSDEAVRLADAEVIIPMVGMVQSLNVSVAGALVLFE
AERQRRATGMYGNRKLPEDYCQKLLFEQGHPIYAKACRRKGLVYPAIDETGQIIAPPDWWQKMREADPDSPRPERGRQRR
M

Sequences:

>Translated_241_residues
MSPERFARINEMLDNRQPDLTVLLDQVHKTNNIAAVLRSADAVGVHQLHAVWPESAMRVSGNTASGSQQWVKTLRHQTVT
DAITALKADGMQVVVTNFSPNAKDFREIDYTKPTAIVLGHEKNGVSDEAVRLADAEVIIPMVGMVQSLNVSVAGALVLFE
AERQRRATGMYGNRKLPEDYCQKLLFEQGHPIYAKACRRKGLVYPAIDETGQIIAPPDWWQKMREADPDSPRPERGRQRR
M
>Mature_240_residues
SPERFARINEMLDNRQPDLTVLLDQVHKTNNIAAVLRSADAVGVHQLHAVWPESAMRVSGNTASGSQQWVKTLRHQTVTD
AITALKADGMQVVVTNFSPNAKDFREIDYTKPTAIVLGHEKNGVSDEAVRLADAEVIIPMVGMVQSLNVSVAGALVLFEA
ERQRRATGMYGNRKLPEDYCQKLLFEQGHPIYAKACRRKGLVYPAIDETGQIIAPPDWWQKMREADPDSPRPERGRQRRM

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=228, Percent_Identity=56.578947368421, Blast_Score=279, Evalue=1e-76,
Organism=Escherichia coli, GI1790623, Length=162, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 26983; Mature: 26852

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPERFARINEMLDNRQPDLTVLLDQVHKTNNIAAVLRSADAVGVHQLHAVWPESAMRVS
CCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCHHEEEC
GNTASGSQQWVKTLRHQTVTDAITALKADGMQVVVTNFSPNAKDFREIDYTKPTAIVLGH
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCEEEEEEC
EKNGVSDEAVRLADAEVIIPMVGMVQSLNVSVAGALVLFEAERQRRATGMYGNRKLPEDY
CCCCCCHHHHHHHCHHHHHHHHHHHHHCCHHHHHEEEEEECHHHHHHCCCCCCCCCCHHH
CQKLLFEQGHPIYAKACRRKGLVYPAIDETGQIIAPPDWWQKMREADPDSPRPERGRQRR
HHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCEECCCHHHHHHHHCCCCCCCCCCHHCCC
M
C
>Mature Secondary Structure 
SPERFARINEMLDNRQPDLTVLLDQVHKTNNIAAVLRSADAVGVHQLHAVWPESAMRVS
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCHHEEEC
GNTASGSQQWVKTLRHQTVTDAITALKADGMQVVVTNFSPNAKDFREIDYTKPTAIVLGH
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCEEEEEEC
EKNGVSDEAVRLADAEVIIPMVGMVQSLNVSVAGALVLFEAERQRRATGMYGNRKLPEDY
CCCCCCHHHHHHHCHHHHHHHHHHHHHCCHHHHHEEEEEECHHHHHHCCCCCCCCCCHHH
CQKLLFEQGHPIYAKACRRKGLVYPAIDETGQIIAPPDWWQKMREADPDSPRPERGRQRR
HHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCEECCCHHHHHHHHCCCCCCCCCCHHCCC
M
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]