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The map label for this gene is glyS

Identifier: 119773167

GI number: 119773167

Start: 23457

End: 25526

Strand: Reverse

Name: glyS

Synonym: Sama_0025

Alternate gene names: 119773167

Gene position: 25526-23457 (Counterclockwise)

Preceding gene: 119773168

Following gene: 119773165

Centisome position: 0.59

GC content: 57.78

Gene sequence:

>2070_bases
ATGAAATTTGAAAACCTGCTCATCGAAATTGGTACCGAAGAGCTGCCACCCAAAGCACTGCGCACCCTGGCCGAATCCTT
TCAAGCCAACTTCAGCGAGGAGCTGACCAAGGCTGAACTGCCATTTGAATCTATCGAATGGTATGCCGCCCCACGTCGCC
TGGCCCTGTACGTTAAGGGGCTGGCAACCGCGCAGGAAGACAAGGTAGTAGAAAAACGCGGGCCTGCGGTGCAGTCCGCC
TTCGATGCCGACGGTAATCCAACCAAGGCCGCCGAAGGCTGGGCCCGTGGTAACGGCATTACCGTGGCAGAGGCCGAGCG
TCTGGCCACTGACAAAGGCGAATGGCTGGTTTATCGTGCCAATGTCGCCGGTGAGCCTACATCTGTTTTGGTGCCTGCGA
TGACCCAGCGCGCACTGGATAAGCTGCCTATCCCCAAGCCGATGCGCTGGGGCAGCAACAGCACTCAGTTTATCCGCCCT
GTGCACACAGTTACCCTGCTGCTTGGCAGCGAACTGATTGCCGGTGAAGTGCTGGGTATTCAATCTGACCGCACTATTCG
TGGCCACCGCTTTATGGGCGAGTCCAGCTTCAGCCTGGATCATGCCGACAACTACCTGGCCGCCCTGAAAGAGCGCGGTA
AAGTTATCGCCGACTATCAGGCCCGTAAAGCCCTCATCAAGGCCGACGCCGAGAAAGCCGCTGCTGTGATTGGTGGCAAC
GCCGACATCGAAGAGAGCCTGCTGGAAGAAGTGGCCTCACTGGTTGAATGGCCAGTGGTACTCACCGCCCACTTTGAAGA
GAAGTTCCTGGCCGTGCCTGCCGAGGCGCTGGTGTACACCATGAAAGGTGACCAGAAGTACTTCCCGGTATTCTCAAACG
ACGGCAAGCTGCTGCCAAACTTCATCTTTGTGGCCAACATCGAGTCCAAAGATCCGGCGCAAATCATTGCCGGTAACGAG
AAGGTGGTTCGTCCACGTCTGGCCGACGCCGAATTCTTCTTCAACACCGACAAAAAACACACCCTTGCAAGCCGTCTGGC
GAGCCTGGAAACCGTAGTATTCCAGAAGCAACTGGGCACCCTGAAAGACCGCGCCGAGCGCATCTCTGCCCTCGCTGGCT
TTATCGCGGGTCAGATTGGCGCCAGCAGCGAAGACGCCGCCCGTGCAGGTTTGCTGTCAAAGTGCGACCTGATGACCAAC
ATGGTGATGGAATTTACCGATACTCAGGGCACCATGGGCATGCACTATGCTCGCCTCGACGGTGAAGCCGAAGCCGTTGC
TGTGGCACTGGAAGAGCAATACAAGCCCAAGTTCAGTGGCGACAGCGTGCCAAGCGCCGGTGTTTCCTGCGCCGTGGCTC
TGGCCGAGAAACTCGACACCCTGTCTGGCATCTTCGGTATCGGTCAGGCGCCGAAAGGCGCTGCTGACCCGTTCGCGCTG
CGCCGTGCTGCCATTGGTGTGCTGCGTATCATAGTTGAGAACAAGCTACCGCTGGATCTGGTTGACCTGATTGCCAAAGC
CGTTGAACTGCACGGTAGCAACCTCAGCAATGCCTTGGCTGCCGATGAAGTGCTTGAGTTCCTGATGGGCCGCTTCCGCG
CCTGGTATCAGGACAAAGGCATCAGCACCGACGTTATTCTTGCCGTATTGGCCCGTCGTCCAACCCGACCTGCGGATTTT
GATGACCGAATTCTGGCGGTAAGCCACTTCCGCACCCTGGAGCAGGCCGCAGCACTTGCCGCCGCCAACAAGCGTGTATC
CAACATCCTGGCCAAGGTGGAAGGTGAACTGCCAAGCAGCATCAATGATGCGCTGCTGAGTGAAGCCGCTGAAAAAGCAC
TGGCAGCCAAGCTTGCCGAAGTAACGCCGGTCATTGCCCCACTGTTCGCTGCCGGTAACTACCAACAGGCTCTGACTGAA
CTGGCTGCCCTGCGCGAGAGCGTGGATCAGTTCTTCGAAGACGTGATGGTCATGGCCGACGACCAGGCGCTTCGCAACAA
CCGTCTGGCACTGCTTGGCAACCTGCGCGACCAGTTCCTGCACGTAGCCGACATTTCTCTGCTTCAATAA

Upstream 100 bases:

>100_bases
CGCACCATGGCCAAGGCGGTTGCCGAAGCCTACTACAAGGCCCGTGAAGCCCTCGGCTTCCCCATGTGCAAATAAGCAGT
GCAAATAAGAGGAAGATTAA

Downstream 100 bases:

>100_bases
TGTAGCAGTAGCAAATGCCCGTCATAAAAAACGCGCCTCAGGGCGCGTTTTTTATTCATCTATATCAACGGGTAAAGAGG
ATGAGTTTTTCGTGCAGGGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MKFENLLIEIGTEELPPKALRTLAESFQANFSEELTKAELPFESIEWYAAPRRLALYVKGLATAQEDKVVEKRGPAVQSA
FDADGNPTKAAEGWARGNGITVAEAERLATDKGEWLVYRANVAGEPTSVLVPAMTQRALDKLPIPKPMRWGSNSTQFIRP
VHTVTLLLGSELIAGEVLGIQSDRTIRGHRFMGESSFSLDHADNYLAALKERGKVIADYQARKALIKADAEKAAAVIGGN
ADIEESLLEEVASLVEWPVVLTAHFEEKFLAVPAEALVYTMKGDQKYFPVFSNDGKLLPNFIFVANIESKDPAQIIAGNE
KVVRPRLADAEFFFNTDKKHTLASRLASLETVVFQKQLGTLKDRAERISALAGFIAGQIGASSEDAARAGLLSKCDLMTN
MVMEFTDTQGTMGMHYARLDGEAEAVAVALEEQYKPKFSGDSVPSAGVSCAVALAEKLDTLSGIFGIGQAPKGAADPFAL
RRAAIGVLRIIVENKLPLDLVDLIAKAVELHGSNLSNALAADEVLEFLMGRFRAWYQDKGISTDVILAVLARRPTRPADF
DDRILAVSHFRTLEQAAALAAANKRVSNILAKVEGELPSSINDALLSEAAEKALAAKLAEVTPVIAPLFAAGNYQQALTE
LAALRESVDQFFEDVMVMADDQALRNNRLALLGNLRDQFLHVADISLLQ

Sequences:

>Translated_689_residues
MKFENLLIEIGTEELPPKALRTLAESFQANFSEELTKAELPFESIEWYAAPRRLALYVKGLATAQEDKVVEKRGPAVQSA
FDADGNPTKAAEGWARGNGITVAEAERLATDKGEWLVYRANVAGEPTSVLVPAMTQRALDKLPIPKPMRWGSNSTQFIRP
VHTVTLLLGSELIAGEVLGIQSDRTIRGHRFMGESSFSLDHADNYLAALKERGKVIADYQARKALIKADAEKAAAVIGGN
ADIEESLLEEVASLVEWPVVLTAHFEEKFLAVPAEALVYTMKGDQKYFPVFSNDGKLLPNFIFVANIESKDPAQIIAGNE
KVVRPRLADAEFFFNTDKKHTLASRLASLETVVFQKQLGTLKDRAERISALAGFIAGQIGASSEDAARAGLLSKCDLMTN
MVMEFTDTQGTMGMHYARLDGEAEAVAVALEEQYKPKFSGDSVPSAGVSCAVALAEKLDTLSGIFGIGQAPKGAADPFAL
RRAAIGVLRIIVENKLPLDLVDLIAKAVELHGSNLSNALAADEVLEFLMGRFRAWYQDKGISTDVILAVLARRPTRPADF
DDRILAVSHFRTLEQAAALAAANKRVSNILAKVEGELPSSINDALLSEAAEKALAAKLAEVTPVIAPLFAAGNYQQALTE
LAALRESVDQFFEDVMVMADDQALRNNRLALLGNLRDQFLHVADISLLQ
>Mature_689_residues
MKFENLLIEIGTEELPPKALRTLAESFQANFSEELTKAELPFESIEWYAAPRRLALYVKGLATAQEDKVVEKRGPAVQSA
FDADGNPTKAAEGWARGNGITVAEAERLATDKGEWLVYRANVAGEPTSVLVPAMTQRALDKLPIPKPMRWGSNSTQFIRP
VHTVTLLLGSELIAGEVLGIQSDRTIRGHRFMGESSFSLDHADNYLAALKERGKVIADYQARKALIKADAEKAAAVIGGN
ADIEESLLEEVASLVEWPVVLTAHFEEKFLAVPAEALVYTMKGDQKYFPVFSNDGKLLPNFIFVANIESKDPAQIIAGNE
KVVRPRLADAEFFFNTDKKHTLASRLASLETVVFQKQLGTLKDRAERISALAGFIAGQIGASSEDAARAGLLSKCDLMTN
MVMEFTDTQGTMGMHYARLDGEAEAVAVALEEQYKPKFSGDSVPSAGVSCAVALAEKLDTLSGIFGIGQAPKGAADPFAL
RRAAIGVLRIIVENKLPLDLVDLIAKAVELHGSNLSNALAADEVLEFLMGRFRAWYQDKGISTDVILAVLARRPTRPADF
DDRILAVSHFRTLEQAAALAAANKRVSNILAKVEGELPSSINDALLSEAAEKALAAKLAEVTPVIAPLFAAGNYQQALTE
LAALRESVDQFFEDVMVMADDQALRNNRLALLGNLRDQFLHVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=68.7953555878084, Blast_Score=951, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_SHEAM (A1S1I1)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_925907.1
- ProteinModelPortal:   A1S1I1
- STRING:   A1S1I1
- GeneID:   4602282
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0025
- NMPDR:   fig|326297.7.peg.22
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   A1S1I1
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74756; Mature: 74756

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFENLLIEIGTEELPPKALRTLAESFQANFSEELTKAELPFESIEWYAAPRRLALYVKG
CCHHHEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHH
LATAQEDKVVEKRGPAVQSAFDADGNPTKAAEGWARGNGITVAEAERLATDKGEWLVYRA
HHCCHHHHHHHHCCCHHHHHCCCCCCCCCHHCCCCCCCCEEEEHHHHHHCCCCCEEEEEC
NVAGEPTSVLVPAMTQRALDKLPIPKPMRWGSNSTQFIRPVHTVTLLLGSELIAGEVLGI
CCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHEEC
QSDRTIRGHRFMGESSFSLDHADNYLAALKERGKVIADYQARKALIKADAEKAAAVIGGN
CCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHEEECCC
ADIEESLLEEVASLVEWPVVLTAHFEEKFLAVPAEALVYTMKGDQKYFPVFSNDGKLLPN
CCHHHHHHHHHHHHHHCCHHEEEEHHHHHHHCCHHHEEEEECCCCEECCEECCCCCCCCC
FIFVANIESKDPAQIIAGNEKVVRPRLADAEFFFNTDKKHTLASRLASLETVVFQKQLGT
EEEEEECCCCCCCCEEECCCCEECCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
LKDRAERISALAGFIAGQIGASSEDAARAGLLSKCDLMTNMVMEFTDTQGTMGMHYARLD
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
GEAEAVAVALEEQYKPKFSGDSVPSAGVSCAVALAEKLDTLSGIFGIGQAPKGAADPFAL
CCHHHHHEEHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGVLRIIVENKLPLDLVDLIAKAVELHGSNLSNALAADEVLEFLMGRFRAWYQDKG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ISTDVILAVLARRPTRPADFDDRILAVSHFRTLEQAAALAAANKRVSNILAKVEGELPSS
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
INDALLSEAAEKALAAKLAEVTPVIAPLFAAGNYQQALTELAALRESVDQFFEDVMVMAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DQALRNNRLALLGNLRDQFLHVADISLLQ
CHHHHCCCEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFENLLIEIGTEELPPKALRTLAESFQANFSEELTKAELPFESIEWYAAPRRLALYVKG
CCHHHEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHH
LATAQEDKVVEKRGPAVQSAFDADGNPTKAAEGWARGNGITVAEAERLATDKGEWLVYRA
HHCCHHHHHHHHCCCHHHHHCCCCCCCCCHHCCCCCCCCEEEEHHHHHHCCCCCEEEEEC
NVAGEPTSVLVPAMTQRALDKLPIPKPMRWGSNSTQFIRPVHTVTLLLGSELIAGEVLGI
CCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHEEC
QSDRTIRGHRFMGESSFSLDHADNYLAALKERGKVIADYQARKALIKADAEKAAAVIGGN
CCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHEEECCC
ADIEESLLEEVASLVEWPVVLTAHFEEKFLAVPAEALVYTMKGDQKYFPVFSNDGKLLPN
CCHHHHHHHHHHHHHHCCHHEEEEHHHHHHHCCHHHEEEEECCCCEECCEECCCCCCCCC
FIFVANIESKDPAQIIAGNEKVVRPRLADAEFFFNTDKKHTLASRLASLETVVFQKQLGT
EEEEEECCCCCCCCEEECCCCEECCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
LKDRAERISALAGFIAGQIGASSEDAARAGLLSKCDLMTNMVMEFTDTQGTMGMHYARLD
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
GEAEAVAVALEEQYKPKFSGDSVPSAGVSCAVALAEKLDTLSGIFGIGQAPKGAADPFAL
CCHHHHHEEHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGVLRIIVENKLPLDLVDLIAKAVELHGSNLSNALAADEVLEFLMGRFRAWYQDKG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ISTDVILAVLARRPTRPADFDDRILAVSHFRTLEQAAALAAANKRVSNILAKVEGELPSS
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
INDALLSEAAEKALAAKLAEVTPVIAPLFAAGNYQQALTELAALRESVDQFFEDVMVMAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DQALRNNRLALLGNLRDQFLHVADISLLQ
CHHHHCCCEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA