Click here to switch to the map view.

The map label for this gene is recF

Identifier: 119773157

GI number: 119773157

Start: 13296

End: 14378

Strand: Direct

Name: recF

Synonym: Sama_0015

Alternate gene names: 119773157

Gene position: 13296-14378 (Clockwise)

Preceding gene: 119773156

Following gene: 119773158

Centisome position: 0.31

GC content: 52.45

Gene sequence:

>1083_bases
ATGAGCCTCAGCCGTCTTTCCATCGACGCATTTCGCAATATTGATTCAGCCCAGCTTGCCCCAGGCGCCGGGCTGAATCT
CATTTATGGCCACAACGGCAGCGGAAAAACCAGTATCCTCGAAGCCATTTACTTTCTGGGTATGGGGCGGTCGTTTAGAA
GCCATTTGTCTCAGCGGGTTATTCAGAACGATGCCGACTGCCTGACGCTGTTTGCCGTAGCCGAAGGGCAGGCCGGTGAC
AGTCGTATCGGTCTGCGGCGGCATCGCAGCGGCGACACCGAAGTCAAAATCGACGGCGAAAAGGTCAAGAGACTGTCGCA
ATTGGCTGAAGCCTTACCTATCCAGGTGATTACGCCAGAGAGCTTTTCGCTGCTGTTTGAGGGGCCATCGGCCAGGCGTC
AGTTTATCGATTGGGGGGCCTTTCATGCCTCCAAACAGTTCCATCTGGCATGGATGAATACACGCAGAATTTTAAAACAA
AGAAATCAGCTGCTTAGAGATGGTGCCAGTTACGAACACATCGCTTTCTGGGACAAGGAATTAATTCGCTATGCCCTTGA
AGTCACAGCGATAAGAAACGATTATGTAGGCTCGTTAAATGGCGTGCTTAAGGGTATAATCGGGGAGTTTTTACCCGATG
TGGATATCCGGGTTTCTTTCACCCGCGGCTGGGACAGCAAGACCGACTTTTCCGAGTTGCTCCAGAGCCAGTATGCGCGG
GATTTGGCCATAGGCCATACGGTCAGCGGACCCCATAAGGCAGACCTCAGATTGCGGGTTGGTAATTTGCCCGCACAGGA
TGCGCTGTCCCGTGGACAATTGAAGTTATTGGTGTGTGCCCTGCGCATTGCCCAGGGCAAGTTGCTTAAGCAACAAATCG
ATAAACACAGCATCTATTTGGTGGACGATCTGCCCTCCGAGTTGGATGCCAAGCACAGGCAGCTGTTGCTGAAGGAACTG
ATAGATACGGGGGCGCAATTGTTTGTCACCGCCATCGAACCTTCAGCCATTGTGGATTCTTTGCCAGTGCCCCCCGACAG
GATGTTTCATGTACAGGCCGGCCGGGTAACACAGACTAAATAA

Upstream 100 bases:

>100_bases
CAACTCCAGCGCCCTGATTGAAAACCACGTGGAAGAAGACTCCATGTACGTGGTTATGCCTATGAGGCTCTAAGACCGTC
ACTGACGAGTCTTTGTTGGT

Downstream 100 bases:

>100_bases
GGAAGGGGCAGGCCCAGGGAGAGGTTATCCCCTGAATTCTCCCAGCATGTTGACGGGAAGATGCACCCTTCCATAACCGA
CGAGAGAGAAATATGTCAGA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSLSRLSIDAFRNIDSAQLAPGAGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRVIQNDADCLTLFAVAEGQAGD
SRIGLRRHRSGDTEVKIDGEKVKRLSQLAEALPIQVITPESFSLLFEGPSARRQFIDWGAFHASKQFHLAWMNTRRILKQ
RNQLLRDGASYEHIAFWDKELIRYALEVTAIRNDYVGSLNGVLKGIIGEFLPDVDIRVSFTRGWDSKTDFSELLQSQYAR
DLAIGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAKHRQLLLKEL
IDTGAQLFVTAIEPSAIVDSLPVPPDRMFHVQAGRVTQTK

Sequences:

>Translated_360_residues
MSLSRLSIDAFRNIDSAQLAPGAGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRVIQNDADCLTLFAVAEGQAGD
SRIGLRRHRSGDTEVKIDGEKVKRLSQLAEALPIQVITPESFSLLFEGPSARRQFIDWGAFHASKQFHLAWMNTRRILKQ
RNQLLRDGASYEHIAFWDKELIRYALEVTAIRNDYVGSLNGVLKGIIGEFLPDVDIRVSFTRGWDSKTDFSELLQSQYAR
DLAIGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAKHRQLLLKEL
IDTGAQLFVTAIEPSAIVDSLPVPPDRMFHVQAGRVTQTK
>Mature_359_residues
SLSRLSIDAFRNIDSAQLAPGAGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRVIQNDADCLTLFAVAEGQAGDS
RIGLRRHRSGDTEVKIDGEKVKRLSQLAEALPIQVITPESFSLLFEGPSARRQFIDWGAFHASKQFHLAWMNTRRILKQR
NQLLRDGASYEHIAFWDKELIRYALEVTAIRNDYVGSLNGVLKGIIGEFLPDVDIRVSFTRGWDSKTDFSELLQSQYARD
LAIGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAKHRQLLLKELI
DTGAQLFVTAIEPSAIVDSLPVPPDRMFHVQAGRVTQTK

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=357, Percent_Identity=44.5378151260504, Blast_Score=325, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_SHEAM (A1S1H1)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_925897.1
- ProteinModelPortal:   A1S1H1
- SMR:   A1S1H1
- STRING:   A1S1H1
- GeneID:   4602272
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0015
- NMPDR:   fig|326297.7.peg.13
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   A1S1H1
- ProtClustDB:   CLSK905538
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40130; Mature: 39998

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSRLSIDAFRNIDSAQLAPGAGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCCCCCCHHHHHCCCCHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
IQNDADCLTLFAVAEGQAGDSRIGLRRHRSGDTEVKIDGEKVKRLSQLAEALPIQVITPE
HHCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCEEEECHHHHHHHHHHHHHCCEEEECCC
SFSLLFEGPSARRQFIDWGAFHASKQFHLAWMNTRRILKQRNQLLRDGASYEHIAFWDKE
CCEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LIRYALEVTAIRNDYVGSLNGVLKGIIGEFLPDVDIRVSFTRGWDSKTDFSELLQSQYAR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
DLAIGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYL
HHHCCCCCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDDLPSELDAKHRQLLLKELIDTGAQLFVTAIEPSAIVDSLPVPPDRMFHVQAGRVTQTK
EECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SLSRLSIDAFRNIDSAQLAPGAGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCCCCCHHHHHCCCCHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
IQNDADCLTLFAVAEGQAGDSRIGLRRHRSGDTEVKIDGEKVKRLSQLAEALPIQVITPE
HHCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCEEEECHHHHHHHHHHHHHCCEEEECCC
SFSLLFEGPSARRQFIDWGAFHASKQFHLAWMNTRRILKQRNQLLRDGASYEHIAFWDKE
CCEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LIRYALEVTAIRNDYVGSLNGVLKGIIGEFLPDVDIRVSFTRGWDSKTDFSELLQSQYAR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
DLAIGHTVSGPHKADLRLRVGNLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYL
HHHCCCCCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDDLPSELDAKHRQLLLKELIDTGAQLFVTAIEPSAIVDSLPVPPDRMFHVQAGRVTQTK
EECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA