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The map label for this gene is gyrB [H]

Identifier: 119773158

GI number: 119773158

Start: 14471

End: 16888

Strand: Direct

Name: gyrB [H]

Synonym: Sama_0016

Alternate gene names: 119773158

Gene position: 14471-16888 (Clockwise)

Preceding gene: 119773157

Following gene: 119773166

Centisome position: 0.34

GC content: 54.3

Gene sequence:

>2418_bases
ATGTCAGAGAATACTTACGATTCGTCGAGTATTAAGGTACTTAAAGGCCTTGATGCAGTACGCAAAAGACCCGGGATGTA
TATCGGTGACACCGACGATGGAACCGGTCTGCATCACATGGTGTTTGAGGTCGTCGACAACTCTATCGACGAAGCCCTGG
CTGGGCATTGCAGCGAAATCACCATCACCATCCATGTGGATGGTTCAGTCTCGGTAAAAGATGACGGCCGTGGTATTCCG
GTAGCCATTCATCCTGAAGAAGGGGTGTCTGCCGCAGAAGTGATCATGACAGTACTGCACGCCGGTGGTAAGTTCGACGA
CAACTCCTATAAGGTGTCCGGTGGTTTGCACGGTGTGGGTGTGTCTGTGGTGAACGCCCTGTCCAAGAAGCTGCAGCTGA
CCATTCGTCGTGACGGCAAGGTACACGAGCAGTTTTACACCCACGGTGTACCTGATGCGCCAATCAAGGTTGTTGGTGAT
GCCACCAAAACCGGTACCGAAGTACGTTTTTGGCCAAGCGAAGAAACCTTCTCCGACACCCTGTTCCATTTCGATATTCT
CGCCAAGCGCGTTCGCGAGCTGTCGTTCCTGAACTCAGGTGTGGGTATTCGTCTGGTTGACGAGCGCGACAACAAAGATG
AATTCTTCAAATATGAAGGTGGTATCAGTGCGTTCGTTGAGTACCTGAACCGCAACAAGACGCCGGTAAACAAGGACATC
TTCCACTTCGCCCAGGAGCGCGAAGACGGTATCACCGTGGAAGTGGCCATGCAGTGGAACGATGGTTTCCAGGAAAACAT
CTACTGTTTTACCAATAACATTCCCCAGCGTGATGGTGGTACCCACCTGGCCGGTTTCCGCGGCGCGCTCACCCGTAACC
TCAACAACTATATGGAAGCTGAGGGTTACAACAAGAAGAACAAAACCAACGCCACCGGTGACGATGCCCGTGAAGGTTTG
ACTGCCGTTATTTCGGTGAAGGTGCCGGATCCCAAGTTCAGCTCTCAGACCAAAGACAAGCTGGTTTCCAGTGAAGTGAA
GTCTGCTGTTGAGCAGACCATGGGTGAGAAGCTCGGCGATTATCTGCTGGAAAACCCAGGTGATGCCAAGCTGATTGTCG
GCAAGATCATCGACGCGGCCCGTGCCCGTGAAGCGGCCCGTAAGGCCCGTGAAATGACCCGCCGTAAAGGCGCGTTGGAC
CTCGGTGGTCTGCCGGGCAAACTGGCAGACTGTCAGGAAAAAGATCCCGGACTCTCCGAAATCTACATAGTGGAGGGTGA
TTCGGCCGGTGGCTCTGCTAAACAAGGCCGAAACCGTAAAAATCAGGCGATTCTGCCACTGAAGGGTAAAATCCTTAACG
TAGAAAAAGCCCGCTTCGACAAGATGCTGTCTTCTCAGGAAGTGGCGACCCTTATCACCGCCTTGGGCTGTGGCATCGGC
CGTGATGAGTACGACCCGGACAAGACCCGTTACCACTACATCATCATCATGACGGATGCTGACGTCGACGGTTCTCACAT
TCGTACCCTGCTGCTGACCTTCTTCTTCCGTCAGATGCCTGAGCTGATTGAGCGCGGCTACGTGTATATCGCCCAGCCAC
CACTGTACAAGGTGAAAAAGGGTAAGCACGAACAGTACCTGAAAGACGATCCAGCCCTGACCGAGTTCCTGACAACGGGT
GCTCTCGACGGCGCTTCTATTCACCCGTCACAGGGCGCACCCGGCATGAGCGGCGAGCCACTGGAAAAGCTGGTGAATCA
GTACCGCGAAGTGGAAGGCATCATCGCCCGTCTGGATCTGCGTTATCCAGAAGTGCTGACCGACCGCATGCTGTATCACC
CAGTGCTGAGCAACGACATTCTGGCTGATCAGGCCAAAGTACAGGCCTGGTGTGATGCCTTTGTGGCCGATCTGGTTGAG
CGTGAAAGCAGCGGCGTACTTTACAACGCCGTGGCCGAGCACGACCCTGAGCGTCAGGTATACGTACCCAAGGTGACTCT
GCGTCGCCACGGTATCGACCATGAGTATCTGTTCAGCTACGACTTCTTCCAGTCAAGCGACTATCGCAAGATGGGCGCTC
TGGCCAACGCACTCGATGGCCTGATTGAAGAGGGCGGTTACGTGCAGCGCGGTGAGAAAGTGAAGCCGGTGACCAGCTTT
ATCGAAGCCCTTGACTGGCTGGTGGGTGAGTCCAAGCGTGGCCTCTACATCCAGCGCTATAAAGGTCTGGGTGAAATGAA
CCCTGAGCAGCTGTGGGAAACCACCATGGATCCTGAGAGCCGCCGTATGCTGCAGGTAACCATTGAAGACGCCATTGCCG
CCGATCAACTGTTTACCTGTTTGATGGGTGATCAGGTTGAGCCGCGCCGTGACTTTATCGAGGCCAACGCTCTTAACGTG
GCTAACCTCGACGTGTAA

Upstream 100 bases:

>100_bases
CTAAATAAGGAAGGGGCAGGCCCAGGGAGAGGTTATCCCCTGAATTCTCCCAGCATGTTGACGGGAAGATGCACCCTTCC
ATAACCGACGAGAGAGAAAT

Downstream 100 bases:

>100_bases
TCGTCCGGTTCGGAAGAAAAAAAGGAAAGCTGCGGCTTTCCTTTTTTGTTCCTTGCATTGGTGTTTTTATCACAGTGCAA
GAGAGGATTGTCAGCCATAG

Product: DNA topoisomerase (ATP-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSEITITIHVDGSVSVKDDGRGIP
VAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRRDGKVHEQFYTHGVPDAPIKVVGD
ATKTGTEVRFWPSEETFSDTLFHFDILAKRVRELSFLNSGVGIRLVDERDNKDEFFKYEGGISAFVEYLNRNKTPVNKDI
FHFAQEREDGITVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRNLNNYMEAEGYNKKNKTNATGDDAREGL
TAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQTMGEKLGDYLLENPGDAKLIVGKIIDAARAREAARKAREMTRRKGALD
LGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYDPDKTRYHYIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLYKVKKGKHEQYLKDDPALTEFLTTG
ALDGASIHPSQGAPGMSGEPLEKLVNQYREVEGIIARLDLRYPEVLTDRMLYHPVLSNDILADQAKVQAWCDAFVADLVE
RESSGVLYNAVAEHDPERQVYVPKVTLRRHGIDHEYLFSYDFFQSSDYRKMGALANALDGLIEEGGYVQRGEKVKPVTSF
IEALDWLVGESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAIAADQLFTCLMGDQVEPRRDFIEANALNV
ANLDV

Sequences:

>Translated_805_residues
MSENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSEITITIHVDGSVSVKDDGRGIP
VAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRRDGKVHEQFYTHGVPDAPIKVVGD
ATKTGTEVRFWPSEETFSDTLFHFDILAKRVRELSFLNSGVGIRLVDERDNKDEFFKYEGGISAFVEYLNRNKTPVNKDI
FHFAQEREDGITVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRNLNNYMEAEGYNKKNKTNATGDDAREGL
TAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQTMGEKLGDYLLENPGDAKLIVGKIIDAARAREAARKAREMTRRKGALD
LGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYDPDKTRYHYIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLYKVKKGKHEQYLKDDPALTEFLTTG
ALDGASIHPSQGAPGMSGEPLEKLVNQYREVEGIIARLDLRYPEVLTDRMLYHPVLSNDILADQAKVQAWCDAFVADLVE
RESSGVLYNAVAEHDPERQVYVPKVTLRRHGIDHEYLFSYDFFQSSDYRKMGALANALDGLIEEGGYVQRGEKVKPVTSF
IEALDWLVGESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAIAADQLFTCLMGDQVEPRRDFIEANALNV
ANLDV
>Mature_804_residues
SENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSEITITIHVDGSVSVKDDGRGIPV
AIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRRDGKVHEQFYTHGVPDAPIKVVGDA
TKTGTEVRFWPSEETFSDTLFHFDILAKRVRELSFLNSGVGIRLVDERDNKDEFFKYEGGISAFVEYLNRNKTPVNKDIF
HFAQEREDGITVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRNLNNYMEAEGYNKKNKTNATGDDAREGLT
AVISVKVPDPKFSSQTKDKLVSSEVKSAVEQTMGEKLGDYLLENPGDAKLIVGKIIDAARAREAARKAREMTRRKGALDL
GGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYDPDKTRYHYIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLYKVKKGKHEQYLKDDPALTEFLTTGA
LDGASIHPSQGAPGMSGEPLEKLVNQYREVEGIIARLDLRYPEVLTDRMLYHPVLSNDILADQAKVQAWCDAFVADLVER
ESSGVLYNAVAEHDPERQVYVPKVTLRRHGIDHEYLFSYDFFQSSDYRKMGALANALDGLIEEGGYVQRGEKVKPVTSFI
EALDWLVGESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAIAADQLFTCLMGDQVEPRRDFIEANALNVA
NLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=565, Percent_Identity=25.3097345132743, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI19913406, Length=576, Percent_Identity=24.6527777777778, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=72.6027397260274, Blast_Score=1204, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=578, Percent_Identity=40.6574394463668, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17535065, Length=552, Percent_Identity=26.0869565217391, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=494, Percent_Identity=24.4939271255061, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI212645657, Length=165, Percent_Identity=32.1212121212121, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=586, Percent_Identity=25.5972696245734, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17136538, Length=542, Percent_Identity=26.9372693726937, Blast_Score=136, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89495; Mature: 89364

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSEI
CCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
TITIHVDGSVSVKDDGRGIPVAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEEEECCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSKKLQLTIRRDGKVHEQFYTHGVPDAPIKVVGDATKTGTEVRFWPSEETFSDT
HHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHH
LFHFDILAKRVRELSFLNSGVGIRLVDERDNKDEFFKYEGGISAFVEYLNRNKTPVNKDI
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHEEECCCHHHHHHHHHCCCCCCCHHH
FHFAQEREDGITVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRNLNNYMEA
HHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHC
EGYNKKNKTNATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQTMGEKLGD
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLLENPGDAKLIVGKIIDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEKDPGLSE
HHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
IYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYDPDKTRYHYIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLYKVKK
CCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHCC
GKHEQYLKDDPALTEFLTTGALDGASIHPSQGAPGMSGEPLEKLVNQYREVEGIIARLDL
CCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
RYPEVLTDRMLYHPVLSNDILADQAKVQAWCDAFVADLVERESSGVLYNAVAEHDPERQV
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCEE
YVPKVTLRRHGIDHEYLFSYDFFQSSDYRKMGALANALDGLIEEGGYVQRGEKVKPVTSF
EECEEHHHHCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
IEALDWLVGESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAIAADQLFTC
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHH
LMGDQVEPRRDFIEANALNVANLDV
HHCCCCCCHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
SENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSEI
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
TITIHVDGSVSVKDDGRGIPVAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEEEECCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSKKLQLTIRRDGKVHEQFYTHGVPDAPIKVVGDATKTGTEVRFWPSEETFSDT
HHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHH
LFHFDILAKRVRELSFLNSGVGIRLVDERDNKDEFFKYEGGISAFVEYLNRNKTPVNKDI
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHEEECCCHHHHHHHHHCCCCCCCHHH
FHFAQEREDGITVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRNLNNYMEA
HHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHC
EGYNKKNKTNATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKSAVEQTMGEKLGD
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLLENPGDAKLIVGKIIDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEKDPGLSE
HHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
IYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYDPDKTRYHYIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLYKVKK
CCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHCC
GKHEQYLKDDPALTEFLTTGALDGASIHPSQGAPGMSGEPLEKLVNQYREVEGIIARLDL
CCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
RYPEVLTDRMLYHPVLSNDILADQAKVQAWCDAFVADLVERESSGVLYNAVAEHDPERQV
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCEE
YVPKVTLRRHGIDHEYLFSYDFFQSSDYRKMGALANALDGLIEEGGYVQRGEKVKPVTSF
EECEEHHHHCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
IEALDWLVGESKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIEDAIAADQLFTC
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHH
LMGDQVEPRRDFIEANALNVANLDV
HHCCCCCCHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]