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The map label for this gene is dnaN [H]

Identifier: 119773156

GI number: 119773156

Start: 12168

End: 13268

Strand: Direct

Name: dnaN [H]

Synonym: Sama_0014

Alternate gene names: 119773156

Gene position: 12168-13268 (Clockwise)

Preceding gene: 119773155

Following gene: 119773157

Centisome position: 0.28

GC content: 54.13

Gene sequence:

>1101_bases
ATGAAATTTTCTATTGATAGGGATGCGCTGTTAAAGCCGTTGCAGCTGGTAAGCGGAGCAGTGGAAAGACGGCATAATCT
GCCTATTCTGGCGAATCTTTTAGTGGAAGTAAGTGGTCACTCACTTAAGATGACCGGCACCGACCTGGAAGTAGAACTCG
TGGGTGAAGCCACGGTTCACGGTGATGTTCAGGAAGGCCGCACCACAGTGCCTGCCAAGAAGTTACTGGATATCGTCAAG
TCCCTGCCAGAGCAGACCGAAATCAAGGTCGAGCAGCAGGAAAACAAATGGTTGCTGCGCTGTGGTCGTAGCCGTTTCTC
CCTGGCGACCCTGCCAGCCGAAGAATACCCCAATGTTGAGGCGTTCCGCCCGGACATCGAATTTACCCTCAAGCAGGGCA
CCCTAAAGTCGCTTATCGACTCCACCCAGTTTTCCATGGCCAACCAGGATGTGCGTTACTACCTTAACGGTCTGTTGATA
GAGACCGAAGGCAATGTACTGCGTGCCGTGGCCACTGATGGCCATCGTCTGGCACTCAGCCATCGCATGATTGAAGCCTC
ATTGCCTGAGAAGCAGGTGATTGTGCCCCGCAAGGGGGTGGTGGAACTGTCGCGCCTGCTCGACGCGGAAGATCAGGACA
TCACCCTGGCCATCGGTGAGAGCGCGATTCGCGCCACGACCGCCACCGCCGTGTTCACCAGTAAGCTGGTGGACGGCCGC
TTCCCTGATTATCGCCGGGTGCTGCCAAAGGGCGGTGACAAGATAGTCATAGCCAGTCGTAATCAGCTTAAACAGGCATT
GCTGCGGGCGTCGATTCTGTCGAACGAGAAATTCCGTGGTGTGCGTATCCTGCTGGAAGATGGCCTGCTTAAAATCACCG
CCAACAATCCCGAGCAGGAAGAGGCCGAAGAAATCATCGACGTGGACTACAACTCCACCACTCTTGAAATCGGCTTTAAC
GTCTCCTATTTGCTGGATGTGCTCAATAGCCTGCGTTCCGATGAAGTGCGTGTCACCCTTATCGACAGCAACTCCAGCGC
CCTGATTGAAAACCACGTGGAAGAAGACTCCATGTACGTGGTTATGCCTATGAGGCTCTAA

Upstream 100 bases:

>100_bases
CGCAAGATCGCCCAGCTGCGCGAAGAGAGCCACGATATTAAAGAAGATTACGCCAACCTGATTCGTACCTTATCTTCCTA
AAAGACAGGGATTGGACACG

Downstream 100 bases:

>100_bases
GACCGTCACTGACGAGTCTTTGTTGGTATGAGCCTCAGCCGTCTTTCCATCGACGCATTTCGCAATATTGATTCAGCCCA
GCTTGCCCCAGGCGCCGGGC

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEATVHGDVQEGRTTVPAKKLLDIVK
SLPEQTEIKVEQQENKWLLRCGRSRFSLATLPAEEYPNVEAFRPDIEFTLKQGTLKSLIDSTQFSMANQDVRYYLNGLLI
ETEGNVLRAVATDGHRLALSHRMIEASLPEKQVIVPRKGVVELSRLLDAEDQDITLAIGESAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNQLKQALLRASILSNEKFRGVRILLEDGLLKITANNPEQEEAEEIIDVDYNSTTLEIGFN
VSYLLDVLNSLRSDEVRVTLIDSNSSALIENHVEEDSMYVVMPMRL

Sequences:

>Translated_366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEATVHGDVQEGRTTVPAKKLLDIVK
SLPEQTEIKVEQQENKWLLRCGRSRFSLATLPAEEYPNVEAFRPDIEFTLKQGTLKSLIDSTQFSMANQDVRYYLNGLLI
ETEGNVLRAVATDGHRLALSHRMIEASLPEKQVIVPRKGVVELSRLLDAEDQDITLAIGESAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNQLKQALLRASILSNEKFRGVRILLEDGLLKITANNPEQEEAEEIIDVDYNSTTLEIGFN
VSYLLDVLNSLRSDEVRVTLIDSNSSALIENHVEEDSMYVVMPMRL
>Mature_366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEATVHGDVQEGRTTVPAKKLLDIVK
SLPEQTEIKVEQQENKWLLRCGRSRFSLATLPAEEYPNVEAFRPDIEFTLKQGTLKSLIDSTQFSMANQDVRYYLNGLLI
ETEGNVLRAVATDGHRLALSHRMIEASLPEKQVIVPRKGVVELSRLLDAEDQDITLAIGESAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNQLKQALLRASILSNEKFRGVRILLEDGLLKITANNPEQEEAEEIIDVDYNSTTLEIGFN
VSYLLDVLNSLRSDEVRVTLIDSNSSALIENHVEEDSMYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=58.1967213114754, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40842; Mature: 40842

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEATVH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCEEEEEEECEEEC
GDVQEGRTTVPAKKLLDIVKSLPEQTEIKVEQQENKWLLRCGRSRFSLATLPAEEYPNVE
CCHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEHCCCCCCEEEECCHHHCCCCE
AFRPDIEFTLKQGTLKSLIDSTQFSMANQDVRYYLNGLLIETEGNVLRAVATDGHRLALS
EECCCCEEEECCCHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCCEEEHH
HRMIEASLPEKQVIVPRKGVVELSRLLDAEDQDITLAIGESAIRATTATAVFTSKLVDGR
HHHHHHCCCCCEEECCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
FPDYRRVLPKGGDKIVIASRNQLKQALLRASILSNEKFRGVRILLEDGLLKITANNPEQE
CCHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHH
EAEEIIDVDYNSTTLEIGFNVSYLLDVLNSLRSDEVRVTLIDSNSSALIENHVEEDSMYV
HHHHHEECCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCCCCCEEE
VMPMRL
EEECCC
>Mature Secondary Structure
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGEATVH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCEEEEEEECEEEC
GDVQEGRTTVPAKKLLDIVKSLPEQTEIKVEQQENKWLLRCGRSRFSLATLPAEEYPNVE
CCHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEHCCCCCCEEEECCHHHCCCCE
AFRPDIEFTLKQGTLKSLIDSTQFSMANQDVRYYLNGLLIETEGNVLRAVATDGHRLALS
EECCCCEEEECCCHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCCEEEHH
HRMIEASLPEKQVIVPRKGVVELSRLLDAEDQDITLAIGESAIRATTATAVFTSKLVDGR
HHHHHHCCCCCEEECCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
FPDYRRVLPKGGDKIVIASRNQLKQALLRASILSNEKFRGVRILLEDGLLKITANNPEQE
CCHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHH
EAEEIIDVDYNSTTLEIGFNVSYLLDVLNSLRSDEVRVTLIDSNSSALIENHVEEDSMYV
HHHHHEECCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCCCCCEEE
VMPMRL
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]