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The map label for this gene is frmA [H]

Identifier: 119773146

GI number: 119773146

Start: 2377

End: 3510

Strand: Reverse

Name: frmA [H]

Synonym: Sama_0004

Alternate gene names: 119773146

Gene position: 3510-2377 (Counterclockwise)

Preceding gene: 119773150

Following gene: 119773145

Centisome position: 0.08

GC content: 57.58

Gene sequence:

>1134_bases
ATGTCTGCATCCTTTATTAAATCCCGCGCCGCCATTGCCTGGGGCCCGGGCCAGCCCTTATCTGTTGAAGAAGTGGATGT
GATGCTGCCCAAGGCAGGCGAAGTGCTGGTGCGTATTGTCGCCACCGGCGTGTGTCACACCGATGCCTTTACCCTGTCGG
GCGACGATCCCGAAGGCGTATTCCCGGCCATTTTGGGTCACGAAGGCGGCGGCATTGTCGAAATGGTGGGCGAAGGCGTG
ACCAGCGTGGCGGTGGGCGACCATGTGATCCCGCTGTACACCGCCGAGTGCGGCAAATGTAAGTTCTGCACCTCGGGCAA
GACCAACCTGTGCTCAGCAGTGCGCGCCACCCAGGGCAAGGGCCTGATGCCAGACGGCACCACCCGCTTCTATAAGGATG
GCGAGCCAATTTATCACTACATGGGCTGCTCCACCTTCAGCGAATACACAGTGCTGCCGGAAATATCCCTCGCCAAGGTC
AACCCCGAAGCGCCACTGGAGGAAGTGTGTCTGCTGGGCTGTGGCGTGACCACAGGTATGGGCGCAGTGCTGAAAACCGC
CAAGGTGAAAAAGGGCGATACTGTGGCCATTTTCGGGCTGGGTGGCATTGGTTTATCGGCGGTGATTGGCGCCACCATGG
CAGGTGCCAGCCGCATCATAGGTATCGATATCAACGACAGTAAGTTTGAACTGGCCAAAAAACTCGGCGCCACCGACTGC
ATCAACCCCAAAGACTACGACAAGCCGATTCAGGACGTGATTGTTGAGATGACCGACGGCGGCGTGGACTTCTCGTTTGA
GTGCATCGGCAATGTGAATGTGATGCGCAGCGCGCTCGAGTGCTGCCACAAGGGCTGGGGCGAATCAGTGATCATCGGCG
TGGCCGGTGCCGGTCAGGAGATTTCCACCCGTCCATTCCAGCTGGTAACCGGCCGGGTATGGCGCGGCAGTGCCTTTGGC
GGCGTAAAAGGACGCTCAGAATTGCCTGGTATTGTTGAGCAGTATCTGCGCGGTGAATTTGCCCTGAGTGACTTTATCAC
CCACACCATGGGACTGGATGCGATTAACGAAGCGTTCGATCTGATGCACGAAGGCAAAAGTATCCGCAGCGTGATCCATT
ACGATCGTAAATAA

Upstream 100 bases:

>100_bases
TCACACACAAAGCGTCTTTGCCATACCCCGGCATCGGCTTTGTACTATCAATAGTGAACCCGGTCATCTGCAAGGCCGCA
AATTCTTGGAAGGAAATCCT

Downstream 100 bases:

>100_bases
GGAGCCGCCATGACGCTGGAATTAGTCAGTGCCAATCGCAGTTTTGATGGCTGGCACAAACAGTACAGTCATCAATCCAG
CAGCCTGGATTGCAAGATGC

Product: alcohol dehydrogenase, zinc-binding

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSASFIKSRAAIAWGPGQPLSVEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEMVGEGV
TSVAVGDHVIPLYTAECGKCKFCTSGKTNLCSAVRATQGKGLMPDGTTRFYKDGEPIYHYMGCSTFSEYTVLPEISLAKV
NPEAPLEEVCLLGCGVTTGMGAVLKTAKVKKGDTVAIFGLGGIGLSAVIGATMAGASRIIGIDINDSKFELAKKLGATDC
INPKDYDKPIQDVIVEMTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSAFG
GVKGRSELPGIVEQYLRGEFALSDFITHTMGLDAINEAFDLMHEGKSIRSVIHYDRK

Sequences:

>Translated_377_residues
MSASFIKSRAAIAWGPGQPLSVEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEMVGEGV
TSVAVGDHVIPLYTAECGKCKFCTSGKTNLCSAVRATQGKGLMPDGTTRFYKDGEPIYHYMGCSTFSEYTVLPEISLAKV
NPEAPLEEVCLLGCGVTTGMGAVLKTAKVKKGDTVAIFGLGGIGLSAVIGATMAGASRIIGIDINDSKFELAKKLGATDC
INPKDYDKPIQDVIVEMTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSAFG
GVKGRSELPGIVEQYLRGEFALSDFITHTMGLDAINEAFDLMHEGKSIRSVIHYDRK
>Mature_376_residues
SASFIKSRAAIAWGPGQPLSVEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEMVGEGVT
SVAVGDHVIPLYTAECGKCKFCTSGKTNLCSAVRATQGKGLMPDGTTRFYKDGEPIYHYMGCSTFSEYTVLPEISLAKVN
PEAPLEEVCLLGCGVTTGMGAVLKTAKVKKGDTVAIFGLGGIGLSAVIGATMAGASRIIGIDINDSKFELAKKLGATDCI
NPKDYDKPIQDVIVEMTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSAFGG
VKGRSELPGIVEQYLRGEFALSDFITHTMGLDAINEAFDLMHEGKSIRSVIHYDRK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=372, Percent_Identity=62.6344086021505, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI34577061, Length=371, Percent_Identity=51.7520215633423, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI4501933, Length=371, Percent_Identity=50.9433962264151, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI71565152, Length=373, Percent_Identity=51.7426273458445, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI4501929, Length=371, Percent_Identity=50.4043126684636, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI156523966, Length=367, Percent_Identity=48.7738419618529, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI262073058, Length=374, Percent_Identity=47.8609625668449, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=49.0304709141274, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=48.2384823848238, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=72.6287262872629, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1787863, Length=354, Percent_Identity=25.7062146892655, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790045, Length=363, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082125, Length=377, Percent_Identity=25.9946949602122, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788073, Length=367, Percent_Identity=26.9754768392371, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081918, Length=228, Percent_Identity=34.6491228070175, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786825, Length=272, Percent_Identity=27.9411764705882, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788075, Length=369, Percent_Identity=24.9322493224932, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788407, Length=228, Percent_Identity=27.1929824561404, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786518, Length=370, Percent_Identity=26.2162162162162, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI226510992, Length=190, Percent_Identity=24.7368421052632, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=375, Percent_Identity=58.4, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71997431, Length=377, Percent_Identity=58.0901856763926, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=58.0174927113703, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17562584, Length=301, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17562876, Length=359, Percent_Identity=25.6267409470752, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988145, Length=219, Percent_Identity=30.1369863013699, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=246, Percent_Identity=29.6747967479675, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=375, Percent_Identity=62.4, Blast_Score=495, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6319621, Length=369, Percent_Identity=29.810298102981, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323729, Length=355, Percent_Identity=30.9859154929577, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324486, Length=355, Percent_Identity=27.887323943662, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323099, Length=376, Percent_Identity=27.9255319148936, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=365, Percent_Identity=27.9452054794521, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319258, Length=236, Percent_Identity=27.5423728813559, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=356, Percent_Identity=26.4044943820225, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319955, Length=356, Percent_Identity=26.4044943820225, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319257, Length=276, Percent_Identity=26.0869565217391, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17737895, Length=379, Percent_Identity=62.0052770448549, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17137530, Length=252, Percent_Identity=32.1428571428571, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17737897, Length=256, Percent_Identity=28.515625, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 39811; Mature: 39680

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASFIKSRAAIAWGPGQPLSVEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGV
CCCHHHHCCCEEEECCCCCCCHHHHEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCC
FPAILGHEGGGIVEMVGEGVTSVAVGDHVIPLYTAECGKCKFCTSGKTNLCSAVRATQGK
CHHEECCCCCCCHHHHCCCCEEEECCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCC
GLMPDGTTRFYKDGEPIYHYMGCSTFSEYTVLPEISLAKVNPEAPLEEVCLLGCGVTTGM
CCCCCCCCCEECCCCCEEEEECCCCCCCEEECCCCEEEECCCCCCHHHHHHHHCCHHHHH
GAVLKTAKVKKGDTVAIFGLGGIGLSAVIGATMAGASRIIGIDINDSKFELAKKLGATDC
HHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCC
INPKDYDKPIQDVIVEMTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQE
CCCCCCCCCHHHHHEEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
ISTRPFQLVTGRVWRGSAFGGVKGRSELPGIVEQYLRGEFALSDFITHTMGLDAINEAFD
CCCCCEEEEECEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHH
LMHEGKSIRSVIHYDRK
HHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
SASFIKSRAAIAWGPGQPLSVEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGV
CCHHHHCCCEEEECCCCCCCHHHHEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCC
FPAILGHEGGGIVEMVGEGVTSVAVGDHVIPLYTAECGKCKFCTSGKTNLCSAVRATQGK
CHHEECCCCCCCHHHHCCCCEEEECCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCC
GLMPDGTTRFYKDGEPIYHYMGCSTFSEYTVLPEISLAKVNPEAPLEEVCLLGCGVTTGM
CCCCCCCCCEECCCCCEEEEECCCCCCCEEECCCCEEEECCCCCCHHHHHHHHCCHHHHH
GAVLKTAKVKKGDTVAIFGLGGIGLSAVIGATMAGASRIIGIDINDSKFELAKKLGATDC
HHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCC
INPKDYDKPIQDVIVEMTDGGVDFSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQE
CCCCCCCCCHHHHHEEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
ISTRPFQLVTGRVWRGSAFGGVKGRSELPGIVEQYLRGEFALSDFITHTMGLDAINEAFD
CCCCCEEEEECEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHH
LMHEGKSIRSVIHYDRK
HHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]