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The map label for this gene is trmE

Identifier: 119773150

GI number: 119773150

Start: 6502

End: 7863

Strand: Reverse

Name: trmE

Synonym: Sama_0008

Alternate gene names: 119773150

Gene position: 7863-6502 (Counterclockwise)

Preceding gene: 119773151

Following gene: 119773146

Centisome position: 0.18

GC content: 57.12

Gene sequence:

>1362_bases
GTGACGACAGACACCATAGTGGCACAGGCCACCGCGCCCGGTCGGGGCGGCGTAGGCATCATCCGGGTATCCGGTAAGCT
GGCAAGCGATGTTGCCCATACCCTGCTGGGACACCTGCCCAAACCCCGCTACGCCGATTTCTGCGACTTCAAAGCGGCCG
ATGGCAGCGTGATTGACCAGGGTATTGCCCTTTTCTTCAAAGGCCCCAACTCCTTTACCGGCGAAGATGTACTGGAGCTG
CAGGGCCACGGCGGCCAGGTGGTGATGGACATGCTCATCCGCGCCGTGCTCAAGGTCAAAGGAGTGCGTATTGCCCGCCC
CGGTGAGTTCAGCGAGCAAGCCTTTATGAACGACAAGCTGGATTTGACCCAGGCCGAAGCCATCGCAGACCTGATTGATG
CCACCAGCGAACAGGCCGCCAAGAGTGCCCTGCACTCACTGCAAGGCGAGTTCTCCACTCAGGTGCATACCCTGGTGGAC
AAAATAACTAACCTGCGCCTGTATGTAGAAGCCGCCATCGACTTTCCCGACGAAGAAGTGGATTTCTTAAGTGACGGCAA
AATAGCCGCCGCCCTTTACGCCATTATCGACCAACTTGATGAAGTGCAGGCCAGTGCAAAACAGGGCAGCATTATCCGTG
AAGGCATGAAAGTGGTGATTGCCGGGCGCCCCAACGCCGGTAAATCCAGCCTGCTCAATGCCCTGGCAGGGAAAGAATCG
GCCATTGTGACCGACATAGCCGGCACCACCCGCGACGTACTGCGAGAGCATATTCACCTCGACGGCATGCCGCTGCACAT
TATTGATACCGCCGGTCTGCGGGACACCACAGACGAAGTGGAGCGTATCGGTATAGAGCGCGCCTGGAGCGAAATAGCCA
GCGCCGATCGGGTGCTGTTTATGGTGGATGGCACAGACACTGCCGCCGTTGACCCCCACGAGATATGGCCCGATTTTATC
GACCGCCTGCCCAAAGCCATGGGGGTTACCGTGGTCCGTAACAAGGCTGATCTGACCGGTGAACCTTTGGAAGCCACCGA
AGAGCAAGGCTACAGCGTGTATCGGATTTCGGCCAAAACCGGCCTGGGTGTGGACGCTCTCAAGCAGCACCTCAAATCCC
TGATGGGTTATCAAAGTAACCTCGAAGGCGGCTTTATCGCCCGTCGTCGTCACCTCGAAGCATTGGAGCAGGCATCTGAG
CACCTGCAACTGGGCAAGGTACAGCTGGAAGTATATCTGGCAGGTGAGCTGCTGGCAGAAGAGCTGCGGATGGCACAGCA
GGCACTGTCTGAAATTACCGGCGAATTTACCTCAGACGACTTGCTGGGCAAGATTTTCAGTTCTTTCTGTATCGGTAAGT
AA

Upstream 100 bases:

>100_bases
AGGCTTAAAATAAGCCCATTTTTCAGGCGAGACACTAAAAAGGCGACCTGAGGTCGCCTTTTTGTTTCTATTTGAAGTAC
ACAATTAAGCGGAAAGACAC

Downstream 100 bases:

>100_bases
GCGGCTTTGCAGAGGATAAAACGCGATTGTCGCACAAAGGCAGTCGCGTTTTTTTATGTGATCGCACCCACCAACAAACA
GCCTCTGATATATCGGGTTC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTTDTIVAQATAPGRGGVGIIRVSGKLASDVAHTLLGHLPKPRYADFCDFKAADGSVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQVVMDMLIRAVLKVKGVRIARPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALHSLQGEFSTQVHTLVD
KITNLRLYVEAAIDFPDEEVDFLSDGKIAAALYAIIDQLDEVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTDIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDEVERIGIERAWSEIASADRVLFMVDGTDTAAVDPHEIWPDFI
DRLPKAMGVTVVRNKADLTGEPLEATEEQGYSVYRISAKTGLGVDALKQHLKSLMGYQSNLEGGFIARRRHLEALEQASE
HLQLGKVQLEVYLAGELLAEELRMAQQALSEITGEFTSDDLLGKIFSSFCIGK

Sequences:

>Translated_453_residues
MTTDTIVAQATAPGRGGVGIIRVSGKLASDVAHTLLGHLPKPRYADFCDFKAADGSVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQVVMDMLIRAVLKVKGVRIARPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALHSLQGEFSTQVHTLVD
KITNLRLYVEAAIDFPDEEVDFLSDGKIAAALYAIIDQLDEVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTDIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDEVERIGIERAWSEIASADRVLFMVDGTDTAAVDPHEIWPDFI
DRLPKAMGVTVVRNKADLTGEPLEATEEQGYSVYRISAKTGLGVDALKQHLKSLMGYQSNLEGGFIARRRHLEALEQASE
HLQLGKVQLEVYLAGELLAEELRMAQQALSEITGEFTSDDLLGKIFSSFCIGK
>Mature_452_residues
TTDTIVAQATAPGRGGVGIIRVSGKLASDVAHTLLGHLPKPRYADFCDFKAADGSVIDQGIALFFKGPNSFTGEDVLELQ
GHGGQVVMDMLIRAVLKVKGVRIARPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALHSLQGEFSTQVHTLVDK
ITNLRLYVEAAIDFPDEEVDFLSDGKIAAALYAIIDQLDEVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKESA
IVTDIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDEVERIGIERAWSEIASADRVLFMVDGTDTAAVDPHEIWPDFID
RLPKAMGVTVVRNKADLTGEPLEATEEQGYSVYRISAKTGLGVDALKQHLKSLMGYQSNLEGGFIARRRHLEALEQASEH
LQLGKVQLEVYLAGELLAEELRMAQQALSEITGEFTSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=34.3283582089552, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI193082988, Length=501, Percent_Identity=31.936127744511, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=33.9869281045752, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=74.7228381374723, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=94, Percent_Identity=38.2978723404255, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=460, Percent_Identity=29.5652173913043, Blast_Score=161, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=31.3883299798793, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=32.9809725158562, Blast_Score=223, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SHEAM (A1S1G4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_925890.1
- ProteinModelPortal:   A1S1G4
- SMR:   A1S1G4
- STRING:   A1S1G4
- GeneID:   4602265
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0008
- NMPDR:   fig|326297.7.peg.8
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   A1S1G4
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48948; Mature: 48817

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 79-79 BINDING 119-119 BINDING 453-453

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTIVAQATAPGRGGVGIIRVSGKLASDVAHTLLGHLPKPRYADFCDFKAADGSVIDQ
CCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHC
GIALFFKGPNSFTGEDVLELQGHGGQVVMDMLIRAVLKVKGVRIARPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALHSLQGEFSTQVHTLVDKITNLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
DFLSDGKIAAALYAIIDQLDEVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTDIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDEVERIGIERAWSEIASADRVLF
EEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
MVDGTDTAAVDPHEIWPDFIDRLPKAMGVTVVRNKADLTGEPLEATEEQGYSVYRISAKT
EEECCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHCCCEEEEEECCC
GLGVDALKQHLKSLMGYQSNLEGGFIARRRHLEALEQASEHLQLGKVQLEVYLAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHH
ELRMAQQALSEITGEFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTDTIVAQATAPGRGGVGIIRVSGKLASDVAHTLLGHLPKPRYADFCDFKAADGSVIDQ
CCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHC
GIALFFKGPNSFTGEDVLELQGHGGQVVMDMLIRAVLKVKGVRIARPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALHSLQGEFSTQVHTLVDKITNLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
DFLSDGKIAAALYAIIDQLDEVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTDIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDEVERIGIERAWSEIASADRVLF
EEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
MVDGTDTAAVDPHEIWPDFIDRLPKAMGVTVVRNKADLTGEPLEATEEQGYSVYRISAKT
EEECCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHCCCEEEEEECCC
GLGVDALKQHLKSLMGYQSNLEGGFIARRRHLEALEQASEHLQLGKVQLEVYLAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHH
ELRMAQQALSEITGEFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA