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The map label for this gene is pyrE

Identifier: 119718573

GI number: 119718573

Start: 4603225

End: 4603764

Strand: Reverse

Name: pyrE

Synonym: Noca_4354

Alternate gene names: 119718573

Gene position: 4603764-4603225 (Counterclockwise)

Preceding gene: 119718575

Following gene: 119718571

Centisome position: 92.34

GC content: 73.52

Gene sequence:

>540_bases
GTGACCCTGGACGCGACGCTCGCCGCTGACATCGACGCCGTCTGCCGCCTGACCGGACAGTTCACCCTGCGGTCGGGTCA
GCAGGCGACCGAGTACTTCGACAAGTACCTGTTCGAGGCCGACCCCCAGCTACTGCTCCGGGTGGCCCGCGAGATGGTCG
GGCTGCTGCCGGACGGCACCGACCTGCTCGGCGGCCTCGAGCTCGGCGGGGTGCCGATCGCCACGATGGTCAGCAGCCTG
ACCGGCCGACCCGCCCTCTACGTCCGCAAGAAGGCCAAGGAGTACGGCACCTGCAAGCTCGCCGAGGGCCCGGACGTCGC
GGGCCGGCGGGTCACCCTGATCGAGGACGTGATCACCACCGGCGGAGCGGTCCGTGACGCCACCCGGGCCCTGCGCGCCG
CCGGCGCGGAGGTCGACGTCGTGGTCTGCGCCATCGACCGCAGCCCGGCCGGCGAGCACCCGCTCGCCGACGTCGGCCTC
GAGGTGCGCCCGGTCCTCACGAAGGCCGAGCTGGACGCGGCTCGCGAGGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
CGCCGACATCTCCGTGAAGATCGTGGGCGGGATCCCCTCGAGGCGGTGGGCGAGCATGTGGCCGACCCTAGTCGGCGCCG
TCTCGGGCTAGGGTTCGGCC

Downstream 100 bases:

>100_bases
GGGCTCGTCCGGCCGGCTGCTGGGCCGGCTGCTGGGCCGGTGGCGTGATCAGTCGCGACGGGTCGCGTCCACGCCGGTGA
TGTCGAGGTCGGGCACGCCG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MTLDATLAADIDAVCRLTGQFTLRSGQQATEYFDKYLFEADPQLLLRVAREMVGLLPDGTDLLGGLELGGVPIATMVSSL
TGRPALYVRKKAKEYGTCKLAEGPDVAGRRVTLIEDVITTGGAVRDATRALRAAGAEVDVVVCAIDRSPAGEHPLADVGL
EVRPVLTKAELDAAREAGA

Sequences:

>Translated_179_residues
MTLDATLAADIDAVCRLTGQFTLRSGQQATEYFDKYLFEADPQLLLRVAREMVGLLPDGTDLLGGLELGGVPIATMVSSL
TGRPALYVRKKAKEYGTCKLAEGPDVAGRRVTLIEDVITTGGAVRDATRALRAAGAEVDVVVCAIDRSPAGEHPLADVGL
EVRPVLTKAELDAAREAGA
>Mature_178_residues
TLDATLAADIDAVCRLTGQFTLRSGQQATEYFDKYLFEADPQLLLRVAREMVGLLPDGTDLLGGLELGGVPIATMVSSLT
GRPALYVRKKAKEYGTCKLAEGPDVAGRRVTLIEDVITTGGAVRDATRALRAAGAEVDVVVCAIDRSPAGEHPLADVGLE
VRPVLTKAELDAAREAGA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=156, Percent_Identity=30.1282051282051, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1790073, Length=147, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508631, Length=156, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554964, Length=153, Percent_Identity=32.6797385620915, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17933654, Length=164, Percent_Identity=32.3170731707317, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_NOCSJ (A1SPW6)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_925538.1
- ProteinModelPortal:   A1SPW6
- SMR:   A1SPW6
- STRING:   A1SPW6
- GeneID:   4596872
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_4354
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   AMVTGKA
- PhylomeDB:   A1SPW6
- ProtClustDB:   CLSK2486794
- BioCyc:   NSP35761:NOCA_4354-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 18851; Mature: 18720

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 24-24 BINDING 89-89 BINDING 90-90 BINDING 93-93 BINDING 119-119 BINDING 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDATLAADIDAVCRLTGQFTLRSGQQATEYFDKYLFEADPQLLLRVAREMVGLLPDGT
CCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
DLLGGLELGGVPIATMVSSLTGRPALYVRKKAKEYGTCKLAEGPDVAGRRVTLIEDVITT
HHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHC
GGAVRDATRALRAAGAEVDVVVCAIDRSPAGEHPLADVGLEVRPVLTKAELDAAREAGA
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHCCCCEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDATLAADIDAVCRLTGQFTLRSGQQATEYFDKYLFEADPQLLLRVAREMVGLLPDGT
CCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
DLLGGLELGGVPIATMVSSLTGRPALYVRKKAKEYGTCKLAEGPDVAGRRVTLIEDVITT
HHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHC
GGAVRDATRALRAAGAEVDVVVCAIDRSPAGEHPLADVGLEVRPVLTKAELDAAREAGA
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHCCCCEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA