Click here to switch to the map view.

The map label for this gene is lysS [H]

Identifier: 119718566

GI number: 119718566

Start: 4596343

End: 4597893

Strand: Reverse

Name: lysS [H]

Synonym: Noca_4347

Alternate gene names: 119718566

Gene position: 4597893-4596343 (Counterclockwise)

Preceding gene: 119718567

Following gene: 119718565

Centisome position: 92.22

GC content: 71.76

Gene sequence:

>1551_bases
GTGACCGAGCACCCCAGCGAGCACCCGTCCGAGCACCCGTCCGAGCACCCAGCGCCCGCCGACGACCTGCCCGACCAGAT
GCGGGTGCGCCGGGCCAAGCGGGCCCGGCTGCTCGAGGAGGGCCGGGAGGCCTACCCCGTCGGCGTGCCGCGCACCCACA
CGCTGGCCGACGTGCGCGAGGCTTGGGGTCACCTCGAGGCCGCGGAGGAGACCCAGGACGTGGTCGGTGTCGCCGGTCGC
GTCGTGTTCGTCCGCAACACCGGCAAGCTGTGCTTCGCGACCCTGCAGGAGGGCATCGGCACCCGGCTCCAGGTGATGCT
CAGCCTCGCCGAGGTCGGCGAGGAGGCGCTCGACGACTGGAAGCACACCGTCGACCTCGGCGACCACGTGTTCGTCGAGG
GCCGGGTGATCTCCTCCAAGCGGGGCGAGCTGTCGGTGATGGCGTCGAGCTGGTCGATGGCCTCCAAGGCGCTGCGCCCG
CTGCCGGTGCTGCACAAGGAGCTGTCCGAGGAGTCGCGGGTCCGCCAGCGCTACGCCGACCTGATCGTGCGCCAGGAGGC
GCGGGACATGGTCCGGACCCGGGCGGCCGTGCTGCGCTCCATCCGGTCGACCCTCGACGACGCGGGCTTCGTCGAGGTCG
AGACGCCGATCCTGCAGATGATGCACGGCGGCGCCGCCGCGCGCCCCTTCGGAACCCACCTGAACGCCTTCGACCAGGAC
ATGACCCTGCGGATCGCTCTCGAGCTGTTCTTGAAGCGCGCCGTCGTCGGCGGCGTGGACCGGGTCTACGAGCTCGGCCG
GATCTTCCGCAACGAGGGCGTCGACTCCACGCACAGCCCCGAGTTCACGATGCTGGAGTTCTACGAGGCGTACGGCGACC
AGTTCACCGCCGCTGAGCGCACCAAGGCGATCGTGCTCAACGCCGCCGCGGCGGCCGGCGTCGGCACCACCCTGGTGGAC
CACCACGGCCACGAGATCGACCTGTCCGGCGAGTGGCGCTGGCTGCCGATCGCCGACGCCGTCTCCGACGCGCTCGGTGA
GCAGATCTCCACCGACGAGACCGCCGACAACGCGGCGGCCCTGCGCCGGCTGGGCGAGGCGCGCGACATCGCGCTCAAGC
CGACCTGGGGCGCCGGCGAGATCATCCTGGAGCTCTACGAACAGCTCGTCGAGCACACCCTGATCCAGCCGACGTTCGTC
TGCGACTACCCCGAGTCGGTGCGCCCGCTCGCCCGGCCGCACCGCACGACGCCCGGCCTGGTCGAGGCCTGGGACCTGAT
CATCGGCGGTATGGAGCTCGCACCGAGCTACTCCGAGCTGGTGGACCCGGTCATCCAGCGCGAGCGGCTGACCGAGCAGT
CCCTGCTCGCGGCCGGCGGCGACGCCGAGGCGATGCAGCTCGACGAGGACTTCCTCCGGGCGCTGGAGTACGGCGCACCC
CCGATGGGCGGCACCGGCATCGGGGTCGACCGCCTCGTGATGCTGCTCACCGGCGTCGGCATCCGGGAGACGATCCTGTT
CCCGCTGCTCAAGCCCGAGGCCGAGCGCTGA

Upstream 100 bases:

>100_bases
GTACGACGAGCTCCTCGGCGACCCGGACGGCGACCCCGACGGCGACCCCGACGGCGACCTGGACGGCTGACCGGCGCGGG
GCCGTCGGTAGCCTTGCCCG

Downstream 100 bases:

>100_bases
CGGTCCGGCGCGGGTAGCCGGCCGGGTCCGCGAGACGCGCCACCGGGACGACCGATCCCCCGATGCGGGTCGTCCCGGCT
CGACCATGCCCCGAGATCGG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTEHPSEHPSEHPSEHPAPADDLPDQMRVRRAKRARLLEEGREAYPVGVPRTHTLADVREAWGHLEAAEETQDVVGVAGR
VVFVRNTGKLCFATLQEGIGTRLQVMLSLAEVGEEALDDWKHTVDLGDHVFVEGRVISSKRGELSVMASSWSMASKALRP
LPVLHKELSEESRVRQRYADLIVRQEARDMVRTRAAVLRSIRSTLDDAGFVEVETPILQMMHGGAAARPFGTHLNAFDQD
MTLRIALELFLKRAVVGGVDRVYELGRIFRNEGVDSTHSPEFTMLEFYEAYGDQFTAAERTKAIVLNAAAAAGVGTTLVD
HHGHEIDLSGEWRWLPIADAVSDALGEQISTDETADNAAALRRLGEARDIALKPTWGAGEIILELYEQLVEHTLIQPTFV
CDYPESVRPLARPHRTTPGLVEAWDLIIGGMELAPSYSELVDPVIQRERLTEQSLLAAGGDAEAMQLDEDFLRALEYGAP
PMGGTGIGVDRLVMLLTGVGIRETILFPLLKPEAER

Sequences:

>Translated_516_residues
MTEHPSEHPSEHPSEHPAPADDLPDQMRVRRAKRARLLEEGREAYPVGVPRTHTLADVREAWGHLEAAEETQDVVGVAGR
VVFVRNTGKLCFATLQEGIGTRLQVMLSLAEVGEEALDDWKHTVDLGDHVFVEGRVISSKRGELSVMASSWSMASKALRP
LPVLHKELSEESRVRQRYADLIVRQEARDMVRTRAAVLRSIRSTLDDAGFVEVETPILQMMHGGAAARPFGTHLNAFDQD
MTLRIALELFLKRAVVGGVDRVYELGRIFRNEGVDSTHSPEFTMLEFYEAYGDQFTAAERTKAIVLNAAAAAGVGTTLVD
HHGHEIDLSGEWRWLPIADAVSDALGEQISTDETADNAAALRRLGEARDIALKPTWGAGEIILELYEQLVEHTLIQPTFV
CDYPESVRPLARPHRTTPGLVEAWDLIIGGMELAPSYSELVDPVIQRERLTEQSLLAAGGDAEAMQLDEDFLRALEYGAP
PMGGTGIGVDRLVMLLTGVGIRETILFPLLKPEAER
>Mature_515_residues
TEHPSEHPSEHPSEHPAPADDLPDQMRVRRAKRARLLEEGREAYPVGVPRTHTLADVREAWGHLEAAEETQDVVGVAGRV
VFVRNTGKLCFATLQEGIGTRLQVMLSLAEVGEEALDDWKHTVDLGDHVFVEGRVISSKRGELSVMASSWSMASKALRPL
PVLHKELSEESRVRQRYADLIVRQEARDMVRTRAAVLRSIRSTLDDAGFVEVETPILQMMHGGAAARPFGTHLNAFDQDM
TLRIALELFLKRAVVGGVDRVYELGRIFRNEGVDSTHSPEFTMLEFYEAYGDQFTAAERTKAIVLNAAAAAGVGTTLVDH
HGHEIDLSGEWRWLPIADAVSDALGEQISTDETADNAAALRRLGEARDIALKPTWGAGEIILELYEQLVEHTLIQPTFVC
DYPESVRPLARPHRTTPGLVEAWDLIIGGMELAPSYSELVDPVIQRERLTEQSLLAAGGDAEAMQLDEDFLRALEYGAPP
MGGTGIGVDRLVMLLTGVGIRETILFPLLKPEAER

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=509, Percent_Identity=37.328094302554, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI194272210, Length=509, Percent_Identity=37.328094302554, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI1790571, Length=508, Percent_Identity=40.748031496063, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789256, Length=505, Percent_Identity=40.1980198019802, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI87082379, Length=324, Percent_Identity=29.320987654321, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788173, Length=154, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=505, Percent_Identity=36.6336633663366, Blast_Score=309, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17535927, Length=505, Percent_Identity=36.6336633663366, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=36.7063492063492, Blast_Score=308, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6320242, Length=529, Percent_Identity=36.8620037807183, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6324256, Length=487, Percent_Identity=28.1314168377823, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24640849, Length=487, Percent_Identity=38.3983572895277, Blast_Score=319, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24640851, Length=489, Percent_Identity=38.241308793456, Blast_Score=318, Evalue=6e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56941; Mature: 56810

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHPSEHPSEHPSEHPAPADDLPDQMRVRRAKRARLLEEGREAYPVGVPRTHTLADVRE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AWGHLEAAEETQDVVGVAGRVVFVRNTGKLCFATLQEGIGTRLQVMLSLAEVGEEALDDW
HHHCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHTVDLGDHVFVEGRVISSKRGELSVMASSWSMASKALRPLPVLHKELSEESRVRQRYAD
HHHHCCCCEEEEEEEEECCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LIVRQEARDMVRTRAAVLRSIRSTLDDAGFVEVETPILQMMHGGAAARPFGTHLNAFDQD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCC
MTLRIALELFLKRAVVGGVDRVYELGRIFRNEGVDSTHSPEFTMLEFYEAYGDQFTAAER
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
TKAIVLNAAAAAGVGTTLVDHHGHEIDLSGEWRWLPIADAVSDALGEQISTDETADNAAA
HHHHEEECHHHHCCCHHHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHH
LRRLGEARDIALKPTWGAGEIILELYEQLVEHTLIQPTFVCDYPESVRPLARPHRTTPGL
HHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCHH
VEAWDLIIGGMELAPSYSELVDPVIQRERLTEQSLLAAGGDAEAMQLDEDFLRALEYGAP
HHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PMGGTGIGVDRLVMLLTGVGIRETILFPLLKPEAER
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEHPSEHPSEHPSEHPAPADDLPDQMRVRRAKRARLLEEGREAYPVGVPRTHTLADVRE
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AWGHLEAAEETQDVVGVAGRVVFVRNTGKLCFATLQEGIGTRLQVMLSLAEVGEEALDDW
HHHCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHTVDLGDHVFVEGRVISSKRGELSVMASSWSMASKALRPLPVLHKELSEESRVRQRYAD
HHHHCCCCEEEEEEEEECCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LIVRQEARDMVRTRAAVLRSIRSTLDDAGFVEVETPILQMMHGGAAARPFGTHLNAFDQD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCC
MTLRIALELFLKRAVVGGVDRVYELGRIFRNEGVDSTHSPEFTMLEFYEAYGDQFTAAER
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
TKAIVLNAAAAAGVGTTLVDHHGHEIDLSGEWRWLPIADAVSDALGEQISTDETADNAAA
HHHHEEECHHHHCCCHHHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHH
LRRLGEARDIALKPTWGAGEIILELYEQLVEHTLIQPTFVCDYPESVRPLARPHRTTPGL
HHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCHH
VEAWDLIIGGMELAPSYSELVDPVIQRERLTEQSLLAAGGDAEAMQLDEDFLRALEYGAP
HHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PMGGTGIGVDRLVMLLTGVGIRETILFPLLKPEAER
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA