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The map label for this gene is purA

Identifier: 119718563

GI number: 119718563

Start: 4592536

End: 4593834

Strand: Reverse

Name: purA

Synonym: Noca_4344

Alternate gene names: 119718563

Gene position: 4593834-4592536 (Counterclockwise)

Preceding gene: 119718565

Following gene: 119718562

Centisome position: 92.14

GC content: 70.21

Gene sequence:

>1299_bases
ATGCCCGCGATCGCCATCATCGGTGCCCAGTGGGGCGACGAGGGGAAGGGCAAGGCGACCGACCTGCTCGGCACCAGCGT
CGACTACGTCGTGAAGTTCAACGGCGGCAACAACGCCGGCCACACGGTCGTCATCACCGACGCCTCCGGGCACACCGAGA
AGTACGCGCTGCACCTGCTGCCCAGCGGCATCCTCACGCCCGGTTGCACGCCCGTGATCGGCAACGGCGTCGTCGTCGAC
CTGGCCGTGCTGTTCGAGGAGCTCGATGCGCTCGAGCAGCGCGGGGTCGACGTGAGCCGGCTCCGGATCAGTGCCAACGC
GCACGTGATCGCGGACTACAACCGCACCCTGGACAAGGTCACCGAGCGCTTCCTCGGCTCGCGGCGCATCGGCACGACCG
GGCGCGGGATCGGCCCGACGTACGCCGACAAGATGAACCGGATCGGCATCCGGGTCCAGGACATCTTCGACGAGAAGATC
CTGACCCAGAAGGTCGAGGGCGTCCTGGACCTGAAGAACCAGGTGCTCACCAAGATCTACAACCGGCGCGCGGCGACCGT
GGAGGCCACCGTCGAGGAGCTGCTCGCGTTCGCCGACCGGCTGCGGCCGATGGTCTGCGACACCGGGCTGCTGCTCAACC
AGGCCCTGGACCGCGGCGAGACCGTGCTGCTCGAGGCCGGCCAGGCCACGCTCCTCGACGTCGACCACGGCACCTACCCG
TTCGTGACGTCCTCCTCGGCCACCGCCGGCGGAGCCTGCACCGGCTCCGGCATCCCACCGCGCCGCCTCGAGCAGGTGAT
CGGCATCGTGAAGGCCTACACCACGCGCGTCGGCGAAGGGCCGTTCCCGACCGAGCTGCACGACGACGCCGGCGAGCACC
TGCGCAAGGTCGGCGCCGAGTTCGGCACCACGACCGGCCGGCCGCGGCGCTGCGGCTGGTACGACGCCGTCATCGCCCGC
TACGCCGCCCGGGTCAACGGCGTCACCGACTTCGTGCTCACCAAGCTCGACGTGCTCACCGGGCTCGAGCAGGTGCCGGT
GTGCGTGGCGTACGACGTGGGCGGGGTGCGCCACGACGAGATGCCGGTCAACCAGACCGACTTCCACCACGCCGTGCCGA
TCTACGAGTACCTCCCCGGTTGGTGGGAGGACATCTCCGGGGCCCGCACGCTCGCCGACCTGCCGGCCAACGCCCAGGCC
TACGTCAAGGCGGTCGAGGAGATGTCCGGTGCCCGGATCTCCGCGGTCGGCGTCGGGCCGTCCCGCGACGCGACCGTCGC
CATCCACGACCTGATCTGA

Upstream 100 bases:

>100_bases
GGTCGCCCTAAGGTATCGTGTGGCCGCAAGAGCTCCGGTGCACTTGTGCCGGAGTTGTTTGTTTCTCCGGCTGTTCCGTT
TCCTGGAAGTGAGCGCGCAG

Downstream 100 bases:

>100_bases
GACCGTTCGGGTCATGCCCGACCCGACTGCCCCCGAACCGGCGGGGCTCGATCCCGCGGCCGCGATCGACCGCCTGCGCG
CGGCGTTCCGCGGCCCCGCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPAIAIIGAQWGDEGKGKATDLLGTSVDYVVKFNGGNNAGHTVVITDASGHTEKYALHLLPSGILTPGCTPVIGNGVVVD
LAVLFEELDALEQRGVDVSRLRISANAHVIADYNRTLDKVTERFLGSRRIGTTGRGIGPTYADKMNRIGIRVQDIFDEKI
LTQKVEGVLDLKNQVLTKIYNRRAATVEATVEELLAFADRLRPMVCDTGLLLNQALDRGETVLLEAGQATLLDVDHGTYP
FVTSSSATAGGACTGSGIPPRRLEQVIGIVKAYTTRVGEGPFPTELHDDAGEHLRKVGAEFGTTTGRPRRCGWYDAVIAR
YAARVNGVTDFVLTKLDVLTGLEQVPVCVAYDVGGVRHDEMPVNQTDFHHAVPIYEYLPGWWEDISGARTLADLPANAQA
YVKAVEEMSGARISAVGVGPSRDATVAIHDLI

Sequences:

>Translated_432_residues
MPAIAIIGAQWGDEGKGKATDLLGTSVDYVVKFNGGNNAGHTVVITDASGHTEKYALHLLPSGILTPGCTPVIGNGVVVD
LAVLFEELDALEQRGVDVSRLRISANAHVIADYNRTLDKVTERFLGSRRIGTTGRGIGPTYADKMNRIGIRVQDIFDEKI
LTQKVEGVLDLKNQVLTKIYNRRAATVEATVEELLAFADRLRPMVCDTGLLLNQALDRGETVLLEAGQATLLDVDHGTYP
FVTSSSATAGGACTGSGIPPRRLEQVIGIVKAYTTRVGEGPFPTELHDDAGEHLRKVGAEFGTTTGRPRRCGWYDAVIAR
YAARVNGVTDFVLTKLDVLTGLEQVPVCVAYDVGGVRHDEMPVNQTDFHHAVPIYEYLPGWWEDISGARTLADLPANAQA
YVKAVEEMSGARISAVGVGPSRDATVAIHDLI
>Mature_431_residues
PAIAIIGAQWGDEGKGKATDLLGTSVDYVVKFNGGNNAGHTVVITDASGHTEKYALHLLPSGILTPGCTPVIGNGVVVDL
AVLFEELDALEQRGVDVSRLRISANAHVIADYNRTLDKVTERFLGSRRIGTTGRGIGPTYADKMNRIGIRVQDIFDEKIL
TQKVEGVLDLKNQVLTKIYNRRAATVEATVEELLAFADRLRPMVCDTGLLLNQALDRGETVLLEAGQATLLDVDHGTYPF
VTSSSATAGGACTGSGIPPRRLEQVIGIVKAYTTRVGEGPFPTELHDDAGEHLRKVGAEFGTTTGRPRRCGWYDAVIARY
AARVNGVTDFVLTKLDVLTGLEQVPVCVAYDVGGVRHDEMPVNQTDFHHAVPIYEYLPGWWEDISGARTLADLPANAQAY
VKAVEEMSGARISAVGVGPSRDATVAIHDLI

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=432, Percent_Identity=44.4444444444444, Blast_Score=344, Evalue=7e-95,
Organism=Homo sapiens, GI34577063, Length=434, Percent_Identity=43.778801843318, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI40316944, Length=403, Percent_Identity=43.6724565756824, Blast_Score=308, Evalue=5e-84,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=48.018648018648, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146553, Length=437, Percent_Identity=42.79176201373, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI25146555, Length=437, Percent_Identity=42.79176201373, Blast_Score=318, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=41.3394919168591, Blast_Score=357, Evalue=3e-99,
Organism=Drosophila melanogaster, GI21356033, Length=428, Percent_Identity=42.7570093457944, Blast_Score=339, Evalue=3e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_NOCSJ (A1SPV6)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_925528.1
- ProteinModelPortal:   A1SPV6
- SMR:   A1SPV6
- STRING:   A1SPV6
- GeneID:   4596862
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_4344
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   A1SPV6
- ProtClustDB:   PRK01117
- BioCyc:   NSP35761:NOCA_4344-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46425; Mature: 46294

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 133-133 BINDING 147-147 BINDING 228-228 BINDING 243-243 BINDING 307-307 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIAIIGAQWGDEGKGKATDLLGTSVDYVVKFNGGNNAGHTVVITDASGHTEKYALHLL
CCCEEEEECCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCCCEEEEEEC
PSGILTPGCTPVIGNGVVVDLAVLFEELDALEQRGVDVSRLRISANAHVIADYNRTLDKV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCEEEECCHHHHHHH
TERFLGSRRIGTTGRGIGPTYADKMNRIGIRVQDIFDEKILTQKVEGVLDLKNQVLTKIY
HHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRRAATVEATVEELLAFADRLRPMVCDTGLLLNQALDRGETVLLEAGQATLLDVDHGTYP
HCCHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCEEEEECCCEEEEECCCCCCC
FVTSSSATAGGACTGSGIPPRRLEQVIGIVKAYTTRVGEGPFPTELHDDAGEHLRKVGAE
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
FGTTTGRPRRCGWYDAVIARYAARVNGVTDFVLTKLDVLTGLEQVPVCVAYDVGGVRHDE
HCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHCCEEEEEECCCCCCCC
MPVNQTDFHHAVPIYEYLPGWWEDISGARTLADLPANAQAYVKAVEEMSGARISAVGVGP
CCCCCCCCCCCCCHHHHCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCC
SRDATVAIHDLI
CCCCEEEEEECC
>Mature Secondary Structure 
PAIAIIGAQWGDEGKGKATDLLGTSVDYVVKFNGGNNAGHTVVITDASGHTEKYALHLL
CCEEEEECCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCCCEEEEEEC
PSGILTPGCTPVIGNGVVVDLAVLFEELDALEQRGVDVSRLRISANAHVIADYNRTLDKV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCEEEECCHHHHHHH
TERFLGSRRIGTTGRGIGPTYADKMNRIGIRVQDIFDEKILTQKVEGVLDLKNQVLTKIY
HHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRRAATVEATVEELLAFADRLRPMVCDTGLLLNQALDRGETVLLEAGQATLLDVDHGTYP
HCCHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCEEEEECCCEEEEECCCCCCC
FVTSSSATAGGACTGSGIPPRRLEQVIGIVKAYTTRVGEGPFPTELHDDAGEHLRKVGAE
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
FGTTTGRPRRCGWYDAVIARYAARVNGVTDFVLTKLDVLTGLEQVPVCVAYDVGGVRHDE
HCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHCCEEEEEECCCCCCCC
MPVNQTDFHHAVPIYEYLPGWWEDISGARTLADLPANAQAYVKAVEEMSGARISAVGVGP
CCCCCCCCCCCCCHHHHCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCC
SRDATVAIHDLI
CCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA