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The map label for this gene is miaB

Identifier: 119718067

GI number: 119718067

Start: 4065829

End: 4067319

Strand: Reverse

Name: miaB

Synonym: Noca_3847

Alternate gene names: 119718067

Gene position: 4067319-4065829 (Counterclockwise)

Preceding gene: 119718070

Following gene: 119718063

Centisome position: 81.58

GC content: 71.9

Gene sequence:

>1491_bases
ATGTCGACTGCGCAGCACACCTCCCCCCGGACATATGAGGTCCGCACCTACGGGTGCCAGATGAACGTCCACGACTCCGA
GCGGCTCACCGGGCTGCTGGAGGACGCGGGCTACGTGGCTGCGCCCGACGGCCAGCAGGCCGACGTGGTCGTGTTCAACA
CCTGCGCGGTCCGGGAGAACGCCGACAACAAGCTCTACGGCAATCTCGGCCACCTGGCACCGGTCAAGGCCGCGACACCG
GGGATGCAGATCGCCGTCGGCGGCTGCCTGGCGCAGAAGGACCGCGACACGATCACCACCCGCGCACCCTGGGTCGACGT
GGTGTTCGGCACCCACAACATCGGTTCGCTGCCGGCGCTGCTCGAGCGGGCGCGGGTGCAGGACGAGGCCCAGGTCGAGA
TCCTCGAGTCGCTGGAGGTGTTCCCCTCGACGCTGCCGACCAGGCGCGAGTCGGCGTACGCCGCCTGGGTGTCGGTGTCG
GTCGGCTGCAACAACACCTGCACGTTCTGCATCGTCCCCAGCCTGCGCGGCAAGGAGAAGGACCGGCGCCCGGGCGAGAT
CCTCGCGGAGGTCGAGGCGCTGGTCGCCGACGGCGTCTCGGAGATCACCCTGCTGGGCCAGAACGTCAACGCCTACGGCG
TGGAGTTCGGTGACCGGCAGGCCTTCTCCAAGCTGCTGCGGGCGTGCGGGGAGGTCGACGGTCTGGAGCGGGTGCGGTTC
ACCAGCCCACACCCGGCCGAGTTCACCGACGACGTCATCGCGGCGATGGCCGAGACGCCGAACGTGATGCCGCAGCTGCA
CATGCCGCTGCAGTCGGGGTCGGACAAGGTGCTGCGCGACATGCGACGGTCCTACCGGCAGCGGAAGTTCCTCGGCATCC
TCGAGCGGGTCCGGGCCGCGATGCCGGACGCCGCGATCACCACCGACATCATCGTCGGCTTCCCCGGCGAGACCGAGGAG
GACTTCCTCCAGACCATGCACGTGGTGCGCGCGGCGCGCTTCTCGGGGGCGTTCACGTTCCAGTACTCCAAGCGTCCCGG
CACCCCCGCCGCCACGCTGCCCGACCAGGTCCCGCCCGAGGTCGTCAAGGACCGCTACGAGCGGCTGGTCGCGCTGGTCA
ACGAGATCGCGTGGGAGGAGAACAAGCGCCTGGTCGGCCGCCGGGTCGAGCTGATGGTCGCCGAGGGCGAGGGCCGCAAG
GACGCCGCCACCCACCGCCTGTCGGGGCGCGGCCCGGACAACCGGCTGGTGCACTTCGCGCCGCCCGCGGGCGTCGAGAT
CCGCCCGGGCGACCTGGCCACGGTCGAGGTCACCTACGCGGCGCCGCACCACCTCGTGGCCGACGGGCCCGTGGTCGACG
TACGCCGTACCCGCTCCGGCGACGCCTGGGCGGCCCGGAACGCCGCGCCGGCCCCGTCGTCCGGCGTCACCCTCGGCATG
CCGACCGTCGGCGCCCCGGCACCGCTCCCGGACGCGCCCGCCTGCCGCTGA

Upstream 100 bases:

>100_bases
AGGAGAGGACCGAGCCCCGCGAGCCGTAGGACCACATGGACCGCACGGTACGCCGCGGTTCGGTCCGGCAGGCCGGCCCG
CCGTACCCTTGACGACTGTC

Downstream 100 bases:

>100_bases
GCCGGCGGTCCCGCGGAGTCAGACCTCGTCGTCCGAGCGCGGGTCCTTGCTGGAGTAGCCGGCCTTGGGCGGCAGGCCGA
CCGGGTTCTCCTCGGGGTTG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSTAQHTSPRTYEVRTYGCQMNVHDSERLTGLLEDAGYVAAPDGQQADVVVFNTCAVRENADNKLYGNLGHLAPVKAATP
GMQIAVGGCLAQKDRDTITTRAPWVDVVFGTHNIGSLPALLERARVQDEAQVEILESLEVFPSTLPTRRESAYAAWVSVS
VGCNNTCTFCIVPSLRGKEKDRRPGEILAEVEALVADGVSEITLLGQNVNAYGVEFGDRQAFSKLLRACGEVDGLERVRF
TSPHPAEFTDDVIAAMAETPNVMPQLHMPLQSGSDKVLRDMRRSYRQRKFLGILERVRAAMPDAAITTDIIVGFPGETEE
DFLQTMHVVRAARFSGAFTFQYSKRPGTPAATLPDQVPPEVVKDRYERLVALVNEIAWEENKRLVGRRVELMVAEGEGRK
DAATHRLSGRGPDNRLVHFAPPAGVEIRPGDLATVEVTYAAPHHLVADGPVVDVRRTRSGDAWAARNAAPAPSSGVTLGM
PTVGAPAPLPDAPACR

Sequences:

>Translated_496_residues
MSTAQHTSPRTYEVRTYGCQMNVHDSERLTGLLEDAGYVAAPDGQQADVVVFNTCAVRENADNKLYGNLGHLAPVKAATP
GMQIAVGGCLAQKDRDTITTRAPWVDVVFGTHNIGSLPALLERARVQDEAQVEILESLEVFPSTLPTRRESAYAAWVSVS
VGCNNTCTFCIVPSLRGKEKDRRPGEILAEVEALVADGVSEITLLGQNVNAYGVEFGDRQAFSKLLRACGEVDGLERVRF
TSPHPAEFTDDVIAAMAETPNVMPQLHMPLQSGSDKVLRDMRRSYRQRKFLGILERVRAAMPDAAITTDIIVGFPGETEE
DFLQTMHVVRAARFSGAFTFQYSKRPGTPAATLPDQVPPEVVKDRYERLVALVNEIAWEENKRLVGRRVELMVAEGEGRK
DAATHRLSGRGPDNRLVHFAPPAGVEIRPGDLATVEVTYAAPHHLVADGPVVDVRRTRSGDAWAARNAAPAPSSGVTLGM
PTVGAPAPLPDAPACR
>Mature_495_residues
STAQHTSPRTYEVRTYGCQMNVHDSERLTGLLEDAGYVAAPDGQQADVVVFNTCAVRENADNKLYGNLGHLAPVKAATPG
MQIAVGGCLAQKDRDTITTRAPWVDVVFGTHNIGSLPALLERARVQDEAQVEILESLEVFPSTLPTRRESAYAAWVSVSV
GCNNTCTFCIVPSLRGKEKDRRPGEILAEVEALVADGVSEITLLGQNVNAYGVEFGDRQAFSKLLRACGEVDGLERVRFT
SPHPAEFTDDVIAAMAETPNVMPQLHMPLQSGSDKVLRDMRRSYRQRKFLGILERVRAAMPDAAITTDIIVGFPGETEED
FLQTMHVVRAARFSGAFTFQYSKRPGTPAATLPDQVPPEVVKDRYERLVALVNEIAWEENKRLVGRRVELMVAEGEGRKD
AATHRLSGRGPDNRLVHFAPPAGVEIRPGDLATVEVTYAAPHHLVADGPVVDVRRTRSGDAWAARNAAPAPSSGVTLGMP
TVGAPAPLPDAPACR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=35.0210970464135, Blast_Score=280, Evalue=3e-75,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=35.0210970464135, Blast_Score=280, Evalue=3e-75,
Organism=Homo sapiens, GI93277076, Length=394, Percent_Identity=28.6802030456853, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=39.7727272727273, Blast_Score=324, Evalue=9e-90,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=31.981981981982, Blast_Score=199, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17553146, Length=466, Percent_Identity=32.4034334763949, Blast_Score=235, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=215, Percent_Identity=36.2790697674419, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356207, Length=428, Percent_Identity=34.5794392523364, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI19922432, Length=398, Percent_Identity=31.1557788944724, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_NOCSJ (A1SNG0)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_925032.1
- ProteinModelPortal:   A1SNG0
- SMR:   A1SNG0
- STRING:   A1SNG0
- GeneID:   4595912
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3847
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- PhylomeDB:   A1SNG0
- ProtClustDB:   PRK14326
- BioCyc:   NSP35761:NOCA_3847-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53819; Mature: 53687

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAQHTSPRTYEVRTYGCQMNVHDSERLTGLLEDAGYVAAPDGQQADVVVFNTCAVREN
CCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCEEEEEEEEECCC
ADNKLYGNLGHLAPVKAATPGMQIAVGGCLAQKDRDTITTRAPWVDVVFGTHNIGSLPAL
CCCCEECCCCCCCCCCCCCCCCEEEECCHHCCCCCCCEEECCCEEEEEECCCCCCHHHHH
LERARVQDEAQVEILESLEVFPSTLPTRRESAYAAWVSVSVGCNNTCTFCIVPSLRGKEK
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCC
DRRPGEILAEVEALVADGVSEITLLGQNVNAYGVEFGDRQAFSKLLRACGEVDGLERVRF
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHEEE
TSPHPAEFTDDVIAAMAETPNVMPQLHMPLQSGSDKVLRDMRRSYRQRKFLGILERVRAA
CCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MPDAAITTDIIVGFPGETEEDFLQTMHVVRAARFSGAFTFQYSKRPGTPAATLPDQVPPE
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHH
VVKDRYERLVALVNEIAWEENKRLVGRRVELMVAEGEGRKDAATHRLSGRGPDNRLVHFA
HHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCCHHHHHCCCCCCCCCCEEEEC
PPAGVEIRPGDLATVEVTYAAPHHLVADGPVVDVRRTRSGDAWAARNAAPAPSSGVTLGM
CCCCCEECCCCEEEEEEEECCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECC
PTVGAPAPLPDAPACR
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STAQHTSPRTYEVRTYGCQMNVHDSERLTGLLEDAGYVAAPDGQQADVVVFNTCAVREN
CCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCEEEEEEEEECCC
ADNKLYGNLGHLAPVKAATPGMQIAVGGCLAQKDRDTITTRAPWVDVVFGTHNIGSLPAL
CCCCEECCCCCCCCCCCCCCCCEEEECCHHCCCCCCCEEECCCEEEEEECCCCCCHHHHH
LERARVQDEAQVEILESLEVFPSTLPTRRESAYAAWVSVSVGCNNTCTFCIVPSLRGKEK
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCC
DRRPGEILAEVEALVADGVSEITLLGQNVNAYGVEFGDRQAFSKLLRACGEVDGLERVRF
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHEEE
TSPHPAEFTDDVIAAMAETPNVMPQLHMPLQSGSDKVLRDMRRSYRQRKFLGILERVRAA
CCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MPDAAITTDIIVGFPGETEEDFLQTMHVVRAARFSGAFTFQYSKRPGTPAATLPDQVPPE
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHH
VVKDRYERLVALVNEIAWEENKRLVGRRVELMVAEGEGRKDAATHRLSGRGPDNRLVHFA
HHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCCHHHHHCCCCCCCCCCEEEEC
PPAGVEIRPGDLATVEVTYAAPHHLVADGPVVDVRRTRSGDAWAARNAAPAPSSGVTLGM
CCCCCEECCCCEEEEEEEECCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECC
PTVGAPAPLPDAPACR
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA