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The map label for this gene is miaA

Identifier: 119718063

GI number: 119718063

Start: 4063380

End: 4064312

Strand: Reverse

Name: miaA

Synonym: Noca_3843

Alternate gene names: 119718063

Gene position: 4064312-4063380 (Counterclockwise)

Preceding gene: 119718067

Following gene: 119718062

Centisome position: 81.52

GC content: 74.71

Gene sequence:

>933_bases
ATGGCCCCGACCCCAAACCGCACGCCGATCGTGGCCGTGGTGGGTGCGACGGCGTCCGGCAAGACGGGGCTCTCCCTGGA
CCTGGCCGAGCGGCTGCGGGGCGAGATCGTCAACACCGACGCGATGCAGGTCTACCGCGGCATGGACATCGGTACGGCGA
AGCTGCCGGTCGCCGAGCGCCGCGGGATCCCGCACCACCTCCTGGACACGCTCACGGTGCGCGACCCGGCGACGGTCGCG
GAGTTCCAGTCCTGGGCGCGGGCCGAGATCGCGGAGATCCGCGGCCGGGGGCGGACGCCGGTCCTGGTCGGCGGGTCCGC
CCTCTACACCCGGGCGATCCTCGACCGCTTCGAGTTCCCCGGCACCGACGAGGCGGTCCGACGGCGGCTGGAGGCGGAGC
TCGACGCCCTCGGGCCGGCCGCCCTCCACGAGCGGCTGCGCGCCGTCGACCCCGAGGCCGCGACGCGGATCCTGGTCGAG
AACGGCCGGCGCATCGTCCGGGCCCTCGAGGTCGTCGAGATCACGGGCCGGCCGTTCACGGCCTCGCTCCCGACCATGGA
GTACGCCGACCCCCGCACCGTCCAGGTCGGCGTCGACATCGACCGGCCGACGCTCGACGCGCGGATCGTCGCGCGCGTGG
ACGCGATGTTCGCCGCCGGGTTCGTCGAGGAGGTCGAGCGGCTGCTGGCCGAGGGGCTCGCCGAGGGCCGCACCGCGGGT
CGCGCGATCGGCTACCGCGAGGTGGCGTCGTACCTCACGGGCGAGCTCAGCCTCGCCGAGGCGCGCGAGCGCACGGTCAG
CGCGACCCGGCGGTTCTCCCGGCGCCAGGACTCCTGGTTCCGCAAGGACCCGCGGGTCACCTGGATCCGGTACGACGATC
CCGGGCTGGTCGACCGGGCGGTGTCGGTGGTCGCCGCCACACTGGACGGATGA

Upstream 100 bases:

>100_bases
GCGGGGCGTCCGCGCCACCGGGCCCGTGCGGGTCGACCGGTCCCTGGGGCGTGTCCTGGGGGTTGTCCTCGGGCTCGCTC
ATGCTTGCCACACTAACCAC

Downstream 100 bases:

>100_bases
CGAAGACCGTCTACTACACCGCCAGCTCGCTCGACGGCTTCATCGCTGACCCGGACAACTCGCTGGCCTGGCTGCTCAGC
CGCGACATCGACCAGGACGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MAPTPNRTPIVAVVGATASGKTGLSLDLAERLRGEIVNTDAMQVYRGMDIGTAKLPVAERRGIPHHLLDTLTVRDPATVA
EFQSWARAEIAEIRGRGRTPVLVGGSALYTRAILDRFEFPGTDEAVRRRLEAELDALGPAALHERLRAVDPEAATRILVE
NGRRIVRALEVVEITGRPFTASLPTMEYADPRTVQVGVDIDRPTLDARIVARVDAMFAAGFVEEVERLLAEGLAEGRTAG
RAIGYREVASYLTGELSLAEARERTVSATRRFSRRQDSWFRKDPRVTWIRYDDPGLVDRAVSVVAATLDG

Sequences:

>Translated_310_residues
MAPTPNRTPIVAVVGATASGKTGLSLDLAERLRGEIVNTDAMQVYRGMDIGTAKLPVAERRGIPHHLLDTLTVRDPATVA
EFQSWARAEIAEIRGRGRTPVLVGGSALYTRAILDRFEFPGTDEAVRRRLEAELDALGPAALHERLRAVDPEAATRILVE
NGRRIVRALEVVEITGRPFTASLPTMEYADPRTVQVGVDIDRPTLDARIVARVDAMFAAGFVEEVERLLAEGLAEGRTAG
RAIGYREVASYLTGELSLAEARERTVSATRRFSRRQDSWFRKDPRVTWIRYDDPGLVDRAVSVVAATLDG
>Mature_309_residues
APTPNRTPIVAVVGATASGKTGLSLDLAERLRGEIVNTDAMQVYRGMDIGTAKLPVAERRGIPHHLLDTLTVRDPATVAE
FQSWARAEIAEIRGRGRTPVLVGGSALYTRAILDRFEFPGTDEAVRRRLEAELDALGPAALHERLRAVDPEAATRILVEN
GRRIVRALEVVEITGRPFTASLPTMEYADPRTVQVGVDIDRPTLDARIVARVDAMFAAGFVEEVERLLAEGLAEGRTAGR
AIGYREVASYLTGELSLAEARERTVSATRRFSRRQDSWFRKDPRVTWIRYDDPGLVDRAVSVVAATLDG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=316, Percent_Identity=31.3291139240506, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1790613, Length=304, Percent_Identity=39.8026315789474, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25144712, Length=306, Percent_Identity=26.1437908496732, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=291, Percent_Identity=27.8350515463918, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24649830, Length=270, Percent_Identity=33.7037037037037, Blast_Score=138, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_NOCSJ (A1SNF6)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_925028.1
- ProteinModelPortal:   A1SNF6
- SMR:   A1SNF6
- STRING:   A1SNF6
- GeneID:   4595908
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3843
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A1SNF6
- BioCyc:   NSP35761:NOCA_3843-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33993; Mature: 33862

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPTPNRTPIVAVVGATASGKTGLSLDLAERLRGEIVNTDAMQVYRGMDIGTAKLPVAER
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHH
RGIPHHLLDTLTVRDPATVAEFQSWARAEIAEIRGRGRTPVLVGGSALYTRAILDRFEFP
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCC
GTDEAVRRRLEAELDALGPAALHERLRAVDPEAATRILVENGRRIVRALEVVEITGRPFT
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHEECCCCCC
ASLPTMEYADPRTVQVGVDIDRPTLDARIVARVDAMFAAGFVEEVERLLAEGLAEGRTAG
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RAIGYREVASYLTGELSLAEARERTVSATRRFSRRQDSWFRKDPRVTWIRYDDPGLVDRA
HHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
VSVVAATLDG
HHHHHHHCCC
>Mature Secondary Structure 
APTPNRTPIVAVVGATASGKTGLSLDLAERLRGEIVNTDAMQVYRGMDIGTAKLPVAER
CCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHH
RGIPHHLLDTLTVRDPATVAEFQSWARAEIAEIRGRGRTPVLVGGSALYTRAILDRFEFP
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCC
GTDEAVRRRLEAELDALGPAALHERLRAVDPEAATRILVENGRRIVRALEVVEITGRPFT
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHEECCCCCC
ASLPTMEYADPRTVQVGVDIDRPTLDARIVARVDAMFAAGFVEEVERLLAEGLAEGRTAG
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RAIGYREVASYLTGELSLAEARERTVSATRRFSRRQDSWFRKDPRVTWIRYDDPGLVDRA
HHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
VSVVAATLDG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA