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The map label for this gene is sdhA [C]

Identifier: 119717770

GI number: 119717770

Start: 3761334

End: 3763199

Strand: Reverse

Name: sdhA [C]

Synonym: Noca_3548

Alternate gene names: 119717770

Gene position: 3763199-3761334 (Counterclockwise)

Preceding gene: 119717771

Following gene: 119717769

Centisome position: 75.48

GC content: 70.79

Gene sequence:

>1866_bases
ATGACTGAACCCCAGACCAGACACCCGATGACGGGCAACGAGATGTCCGGCGCCGAGGCCGGCGGCCTGGAGCGGCACCG
GTACGACGTGGTCGTGGTCGGCGCCGGCGGGGCGGGGCTGCGCGCGGCGATCGCCGCGCACGAGACGGGCGCGCGCACCG
CGATCGTGTGCAAGTCCCTGCTCGGCAAGGCGCACACGGTGATGGCCGAGGGCGGCATCGCGGCGGCGATGGGCAACCGC
TGGCCCGAGGACAACTGGGAGGTGCACTTCCGCGACACCATGCGTGGCGGAAAGATGCTCAACAACTGGCGGATGGCCCA
GCTGCACGCCCAGGAGGCCCCCGAGCGGGTGATGGAGCTCGAGGACTGGGGCGCCCTGTTCGACCGGACCGACGACGGGC
TGATCTCGCAGCGCGACTTCGGTGGCCACAAGTACGCCCGCCTCGCCCACGTCGGCGACCGCACCGGCCTGGAGATGATC
CGCACCCTCCAGCAGCGCGCCGTGGCGCTCGGCATCGACGTGTTCATGGAGTGCACGGTCACCGAGCTGTTGAAGGTGGA
CGGGGCGATCGCCGGCGCGTTCGCGTACTGGCGCGAGACCGGTCGGTTCGTCGTCTTCGAGGCACCGTCCGTGGTGCTCG
CGACCGGCGGGATCGGCAAGTCGTTCAAGGTCACGTCGAACTCGTGGGAGTACACCGGCGACGGCCACGCCCTGGCGCTC
CGCGCCGGGGCCGCCCTGATCAACATGGAGTTCGTCCAGTTCCACCCCACGGGCATGGTCTGGCCGCCGTCCGTGAAGGG
GCTGCTGGTCACCGAGTCGGTGCGCGGCGACGGCGGCATCCTGAAGAACTCCGAAGGCCAGCGCTTCATGTTCGACTACA
TCCCGGAGTACTTCCGGGACGAGACGGCGGACACGGTCGAGGAGGCCGACCGCTGGTACGACGACAAGGCGAACAGCCGC
CGCCCGCCCGAGCTGCTGCCGCGCGACGAGGTGGCCCGCGCGATCAACTCCGAGATCAAGGCCGGCCGGGGCACCCCGCA
CGGCGGCATCTACCTCGACATCGCCTCCCGCCGGTCCCCCGAGTTCATCCGCAAGCGGCTCCCGTCGATGTACCACCAGT
TCAAGGAGCTGGCCGACGTCGACATCACCCAGGAGCCGATGGAGATCGGGCCGACCTGCCACTACGTGATGGGCGGCATC
GAGGTGGACGCCGACACCGAGCTGTCGGCGGTGCCGGGCCTGTACGCCGTGGGCGAGTGCTCGGGCGGCATGCACGGCTC
CAACCGGCTCGGCGGCAACTCCCTCTCCGACCTGCTGGTGTTCGGCCGTCGGGCCGGCGAGTCTGCGGCGTCGTACTCCA
CCTCGCTCGGCGGGTCCCGCCCGGCGGTGCGGGACGAGGACGTGAAGGCGGCCGAGCGTGACGCGCTCGCGCCGTTCGAG
GTCACCGCATCGGAGGGTGGCGGGGAGAACCCCTACACGGTCCAGCAGGACCTGCAGCAGTCGATGAACGACCTGGTCGG
CATCATCCGCACCCGCCCCGAGCTGGAGCAGTCGCTCCAGGAGATCGACCGGCTCAAGGAGCGGGCCCGGCGGATGACGG
TCGAGGGGCACCGGCAGTACAACCCCGGGTGGCACCTCGCGCTCGACCTGCGCAACATGCTGCTGGTCAGCGAGTGCATC
GCGAAGGCCGCGCTCGAGCGCGAGGAGTCGCGCGGCGGGCACACCCGCGACGACTTCCCGGGGCCCAGCCCCGAGTGGGG
GTCGAAGAACCTGGTGCTCCGGCTGGCCGCCGACCGCACCGGCGTCGACCTCGCCCACCAGCCGCTCCCGGCGATGCCGG
ACGAGCTCAAGAAGTTCTTCGAGTGA

Upstream 100 bases:

>100_bases
TTCGACGACCCGAGGTTCTTTTGAACCACCCCACGAAGTTCCCAGCTCCCCCGCTTCGCTCCTCCGCTGACAACTCCGCG
GGGACCCCGGAAGGCCACAG

Downstream 100 bases:

>100_bases
AGGGGCCGTCATGGGCTACACGCTGAAGATGCGGATCTGGCGCGGCGACCAGGACGGCGGCGACCTCGGCGACTACGACG
TCGAGGTCTCCGAGGGCGAG

Product: succinate dehydrogenase flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTEPQTRHPMTGNEMSGAEAGGLERHRYDVVVVGAGGAGLRAAIAAHETGARTAIVCKSLLGKAHTVMAEGGIAAAMGNR
WPEDNWEVHFRDTMRGGKMLNNWRMAQLHAQEAPERVMELEDWGALFDRTDDGLISQRDFGGHKYARLAHVGDRTGLEMI
RTLQQRAVALGIDVFMECTVTELLKVDGAIAGAFAYWRETGRFVVFEAPSVVLATGGIGKSFKVTSNSWEYTGDGHALAL
RAGAALINMEFVQFHPTGMVWPPSVKGLLVTESVRGDGGILKNSEGQRFMFDYIPEYFRDETADTVEEADRWYDDKANSR
RPPELLPRDEVARAINSEIKAGRGTPHGGIYLDIASRRSPEFIRKRLPSMYHQFKELADVDITQEPMEIGPTCHYVMGGI
EVDADTELSAVPGLYAVGECSGGMHGSNRLGGNSLSDLLVFGRRAGESAASYSTSLGGSRPAVRDEDVKAAERDALAPFE
VTASEGGGENPYTVQQDLQQSMNDLVGIIRTRPELEQSLQEIDRLKERARRMTVEGHRQYNPGWHLALDLRNMLLVSECI
AKAALEREESRGGHTRDDFPGPSPEWGSKNLVLRLAADRTGVDLAHQPLPAMPDELKKFFE

Sequences:

>Translated_621_residues
MTEPQTRHPMTGNEMSGAEAGGLERHRYDVVVVGAGGAGLRAAIAAHETGARTAIVCKSLLGKAHTVMAEGGIAAAMGNR
WPEDNWEVHFRDTMRGGKMLNNWRMAQLHAQEAPERVMELEDWGALFDRTDDGLISQRDFGGHKYARLAHVGDRTGLEMI
RTLQQRAVALGIDVFMECTVTELLKVDGAIAGAFAYWRETGRFVVFEAPSVVLATGGIGKSFKVTSNSWEYTGDGHALAL
RAGAALINMEFVQFHPTGMVWPPSVKGLLVTESVRGDGGILKNSEGQRFMFDYIPEYFRDETADTVEEADRWYDDKANSR
RPPELLPRDEVARAINSEIKAGRGTPHGGIYLDIASRRSPEFIRKRLPSMYHQFKELADVDITQEPMEIGPTCHYVMGGI
EVDADTELSAVPGLYAVGECSGGMHGSNRLGGNSLSDLLVFGRRAGESAASYSTSLGGSRPAVRDEDVKAAERDALAPFE
VTASEGGGENPYTVQQDLQQSMNDLVGIIRTRPELEQSLQEIDRLKERARRMTVEGHRQYNPGWHLALDLRNMLLVSECI
AKAALEREESRGGHTRDDFPGPSPEWGSKNLVLRLAADRTGVDLAHQPLPAMPDELKKFFE
>Mature_620_residues
TEPQTRHPMTGNEMSGAEAGGLERHRYDVVVVGAGGAGLRAAIAAHETGARTAIVCKSLLGKAHTVMAEGGIAAAMGNRW
PEDNWEVHFRDTMRGGKMLNNWRMAQLHAQEAPERVMELEDWGALFDRTDDGLISQRDFGGHKYARLAHVGDRTGLEMIR
TLQQRAVALGIDVFMECTVTELLKVDGAIAGAFAYWRETGRFVVFEAPSVVLATGGIGKSFKVTSNSWEYTGDGHALALR
AGAALINMEFVQFHPTGMVWPPSVKGLLVTESVRGDGGILKNSEGQRFMFDYIPEYFRDETADTVEEADRWYDDKANSRR
PPELLPRDEVARAINSEIKAGRGTPHGGIYLDIASRRSPEFIRKRLPSMYHQFKELADVDITQEPMEIGPTCHYVMGGIE
VDADTELSAVPGLYAVGECSGGMHGSNRLGGNSLSDLLVFGRRAGESAASYSTSLGGSRPAVRDEDVKAAERDALAPFEV
TASEGGGENPYTVQQDLQQSMNDLVGIIRTRPELEQSLQEIDRLKERARRMTVEGHRQYNPGWHLALDLRNMLLVSECIA
KAALEREESRGGHTRDDFPGPSPEWGSKNLVLRLAADRTGVDLAHQPLPAMPDELKKFFE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=556, Percent_Identity=34.1726618705036, Blast_Score=275, Evalue=1e-73,
Organism=Escherichia coli, GI1786942, Length=619, Percent_Identity=36.0258481421648, Blast_Score=297, Evalue=2e-81,
Organism=Escherichia coli, GI1790597, Length=535, Percent_Identity=35.7009345794393, Blast_Score=285, Evalue=8e-78,
Organism=Escherichia coli, GI1788928, Length=587, Percent_Identity=32.8790459965928, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17550100, Length=554, Percent_Identity=35.0180505415162, Blast_Score=282, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17505833, Length=566, Percent_Identity=32.8621908127208, Blast_Score=275, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI71986328, Length=441, Percent_Identity=26.3038548752834, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322701, Length=595, Percent_Identity=35.4621848739496, Blast_Score=301, Evalue=3e-82,
Organism=Saccharomyces cerevisiae, GI6322416, Length=575, Percent_Identity=36, Blast_Score=300, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6320788, Length=480, Percent_Identity=27.5, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322511, Length=485, Percent_Identity=25.7731958762887, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17137288, Length=575, Percent_Identity=34.4347826086956, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24655642, Length=575, Percent_Identity=34.4347826086956, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24655647, Length=575, Percent_Identity=34.4347826086956, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24663005, Length=577, Percent_Identity=34.315424610052, Blast_Score=267, Evalue=2e-71,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 68276; Mature: 68145

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPQTRHPMTGNEMSGAEAGGLERHRYDVVVVGAGGAGLRAAIAAHETGARTAIVCKSL
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
LGKAHTVMAEGGIAAAMGNRWPEDNWEVHFRDTMRGGKMLNNWRMAQLHAQEAPERVMEL
HCCHHEEEECCCEEEHHCCCCCCCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHH
EDWGALFDRTDDGLISQRDFGGHKYARLAHVGDRTGLEMIRTLQQRAVALGIDVFMECTV
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TELLKVDGAIAGAFAYWRETGRFVVFEAPSVVLATGGIGKSFKVTSNSWEYTGDGHALAL
HHHHHHCCHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCEEEEECCCCEECCCCCEEEE
RAGAALINMEFVQFHPTGMVWPPSVKGLLVTESVRGDGGILKNSEGQRFMFDYIPEYFRD
ECCEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEHHHHHHHHHCC
ETADTVEEADRWYDDKANSRRPPELLPRDEVARAINSEIKAGRGTPHGGIYLDIASRRSP
CHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCH
EFIRKRLPSMYHQFKELADVDITQEPMEIGPTCHYVMGGIEVDADTELSAVPGLYAVGEC
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCEEECCCCCHHHCCCEEEEECC
SGGMHGSNRLGGNSLSDLLVFGRRAGESAASYSTSLGGSRPAVRDEDVKAAERDALAPFE
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEE
VTASEGGGENPYTVQQDLQQSMNDLVGIIRTRPELEQSLQEIDRLKERARRMTVEGHRQY
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NPGWHLALDLRNMLLVSECIAKAALEREESRGGHTRDDFPGPSPEWGSKNLVLRLAADRT
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
GVDLAHQPLPAMPDELKKFFE
CCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
TEPQTRHPMTGNEMSGAEAGGLERHRYDVVVVGAGGAGLRAAIAAHETGARTAIVCKSL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
LGKAHTVMAEGGIAAAMGNRWPEDNWEVHFRDTMRGGKMLNNWRMAQLHAQEAPERVMEL
HCCHHEEEECCCEEEHHCCCCCCCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHH
EDWGALFDRTDDGLISQRDFGGHKYARLAHVGDRTGLEMIRTLQQRAVALGIDVFMECTV
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TELLKVDGAIAGAFAYWRETGRFVVFEAPSVVLATGGIGKSFKVTSNSWEYTGDGHALAL
HHHHHHCCHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCEEEEECCCCEECCCCCEEEE
RAGAALINMEFVQFHPTGMVWPPSVKGLLVTESVRGDGGILKNSEGQRFMFDYIPEYFRD
ECCEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEHHHHHHHHHCC
ETADTVEEADRWYDDKANSRRPPELLPRDEVARAINSEIKAGRGTPHGGIYLDIASRRSP
CHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCH
EFIRKRLPSMYHQFKELADVDITQEPMEIGPTCHYVMGGIEVDADTELSAVPGLYAVGEC
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCEEECCCCCHHHCCCEEEEECC
SGGMHGSNRLGGNSLSDLLVFGRRAGESAASYSTSLGGSRPAVRDEDVKAAERDALAPFE
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEE
VTASEGGGENPYTVQQDLQQSMNDLVGIIRTRPELEQSLQEIDRLKERARRMTVEGHRQY
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NPGWHLALDLRNMLLVSECIAKAALEREESRGGHTRDDFPGPSPEWGSKNLVLRLAADRT
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
GVDLAHQPLPAMPDELKKFFE
CCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]