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The map label for this gene is thyA

Identifier: 119716538

GI number: 119716538

Start: 2461315

End: 2462136

Strand: Direct

Name: thyA

Synonym: Noca_2311

Alternate gene names: 119716538

Gene position: 2461315-2462136 (Clockwise)

Preceding gene: 119716537

Following gene: 119716539

Centisome position: 49.37

GC content: 69.83

Gene sequence:

>822_bases
GTGCGGGCCTACCTCGACCTCCTCCAGCGCGTCGTCGACGAAGGCGTCCCCAAGGGCGACCGCACCGGCACCGGGACGCT
GAGCGTCTTCGGGCACCAGATGCGCTTCGACCTGCGCGCCGGGTTCCCGCTCGTGACCACCAAGAAGGTGCACACCCGCT
CGGTCTTCGGGGAGCTGCTGTGGTTCCTGCGCGGCGACACGAACGTGAAGTGGCTCCAGGACCGCGGCATCACGATCTGG
GACGAGTGGGCCGACGAGCAGGGCGACCTGGGACCGGTCTACGGCTACCAGTGGCGCTCCTGGCCGACTCCTGACGGGCG
GCACGTCGACCAGATCGCCCAGGTGGTCGAGCAGATCCGTGACTCCCCGGACTCGCGGCGCCACGTCGTGTCGGCGTGGA
ACGTCGCGGACATCCCCGACATGGCGCTCGCGCCGTGCCACACGATGTTCCAGTTCTACGTCGCGCCGCCTGGACCTGGG
GAGGAGTCCGGGCCGGGGCGGCTCAGCTGCCAGCTCTACCAGCGCTCTGCGGACGTCTTCCTCGGCGTGCCGTTCAACAT
CGCGTCGTACGCCCTGCTCACCCACATGGTCGCGCAGGTGACCGGCCTCGAGGTCGGCGACTTCGTGCACACCCTCGGCG
ATGCGCACCTGTACTCCAACCACCTCGACCAGGCGCGGCTCCAGCTCACGCGGGAGCCGCGGCCGCTGCCCCGGCTGGTG
CTCGACTCGTCGGTGACCGAGCTGGACGCGTTCGACCTCGAGCACATCGCGGTCGAGGGCTACGACCCGCACCCCGGAAT
CAAGGCCCCCATCGCCGTATGA

Upstream 100 bases:

>100_bases
CGGGTGCACGTGGTGAAGGAGGGGCAGACGGTGTGGGTCGGCGGCGACACCCGGACGACGATCAGCGGCTCCGTGGATCT
CTGACGGCTACGCTCGCAAC

Downstream 100 bases:

>100_bases
GCCGGCAGGTGAGCGGCCGATGACCCCCGGCGGCAAGCGCGTCGTCCTCGTCGCCGCCGTGGCGCGCAACGGCGTGATCG
GCGACGGCCCCGACATCCCC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRAYLDLLQRVVDEGVPKGDRTGTGTLSVFGHQMRFDLRAGFPLVTTKKVHTRSVFGELLWFLRGDTNVKWLQDRGITIW
DEWADEQGDLGPVYGYQWRSWPTPDGRHVDQIAQVVEQIRDSPDSRRHVVSAWNVADIPDMALAPCHTMFQFYVAPPGPG
EESGPGRLSCQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLEVGDFVHTLGDAHLYSNHLDQARLQLTREPRPLPRLV
LDSSVTELDAFDLEHIAVEGYDPHPGIKAPIAV

Sequences:

>Translated_273_residues
MRAYLDLLQRVVDEGVPKGDRTGTGTLSVFGHQMRFDLRAGFPLVTTKKVHTRSVFGELLWFLRGDTNVKWLQDRGITIW
DEWADEQGDLGPVYGYQWRSWPTPDGRHVDQIAQVVEQIRDSPDSRRHVVSAWNVADIPDMALAPCHTMFQFYVAPPGPG
EESGPGRLSCQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLEVGDFVHTLGDAHLYSNHLDQARLQLTREPRPLPRLV
LDSSVTELDAFDLEHIAVEGYDPHPGIKAPIAV
>Mature_273_residues
MRAYLDLLQRVVDEGVPKGDRTGTGTLSVFGHQMRFDLRAGFPLVTTKKVHTRSVFGELLWFLRGDTNVKWLQDRGITIW
DEWADEQGDLGPVYGYQWRSWPTPDGRHVDQIAQVVEQIRDSPDSRRHVVSAWNVADIPDMALAPCHTMFQFYVAPPGPG
EESGPGRLSCQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLEVGDFVHTLGDAHLYSNHLDQARLQLTREPRPLPRLV
LDSSVTELDAFDLEHIAVEGYDPHPGIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=290, Percent_Identity=50.3448275862069, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1789191, Length=273, Percent_Identity=65.2014652014652, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71993377, Length=291, Percent_Identity=47.4226804123711, Blast_Score=273, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6324648, Length=290, Percent_Identity=42.4137931034483, Blast_Score=225, Evalue=7e-60,
Organism=Drosophila melanogaster, GI17137556, Length=290, Percent_Identity=51.3793103448276, Blast_Score=292, Evalue=2e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_NOCSJ (A1SJ31)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_923503.1
- ProteinModelPortal:   A1SJ31
- SMR:   A1SJ31
- STRING:   A1SJ31
- GeneID:   4595926
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_2311
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   A1SJ31
- ProtClustDB:   CLSK2533200
- BioCyc:   NSP35761:NOCA_2311-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30593; Mature: 30593

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAYLDLLQRVVDEGVPKGDRTGTGTLSVFGHQMRFDLRAGFPLVTTKKVHTRSVFGELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHEEEECCCCCEEECCHHHHHHHHHHHH
WFLRGDTNVKWLQDRGITIWDEWADEQGDLGPVYGYQWRSWPTPDGRHVDQIAQVVEQIR
HHHCCCCCCEEEECCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
DSPDSRRHVVSAWNVADIPDMALAPCHTMFQFYVAPPGPGEESGPGRLSCQLYQRSADVF
CCCCCHHHEEEECCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCEEEEEEECCCCEE
LGVPFNIASYALLTHMVAQVTGLEVGDFVHTLGDAHLYSNHLDQARLQLTREPRPLPRLV
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHH
LDSSVTELDAFDLEHIAVEGYDPHPGIKAPIAV
HCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRAYLDLLQRVVDEGVPKGDRTGTGTLSVFGHQMRFDLRAGFPLVTTKKVHTRSVFGELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHEEEECCCCCEEECCHHHHHHHHHHHH
WFLRGDTNVKWLQDRGITIWDEWADEQGDLGPVYGYQWRSWPTPDGRHVDQIAQVVEQIR
HHHCCCCCCEEEECCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
DSPDSRRHVVSAWNVADIPDMALAPCHTMFQFYVAPPGPGEESGPGRLSCQLYQRSADVF
CCCCCHHHEEEECCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCEEEEEEECCCCEE
LGVPFNIASYALLTHMVAQVTGLEVGDFVHTLGDAHLYSNHLDQARLQLTREPRPLPRLV
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHH
LDSSVTELDAFDLEHIAVEGYDPHPGIKAPIAV
HCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA