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The map label for this gene is dhfrIII [H]

Identifier: 119716539

GI number: 119716539

Start: 2462156

End: 2462638

Strand: Direct

Name: dhfrIII [H]

Synonym: Noca_2312

Alternate gene names: 119716539

Gene position: 2462156-2462638 (Clockwise)

Preceding gene: 119716538

Following gene: 119716540

Centisome position: 49.38

GC content: 74.33

Gene sequence:

>483_bases
ATGACCCCCGGCGGCAAGCGCGTCGTCCTCGTCGCCGCCGTGGCGCGCAACGGCGTGATCGGCGACGGCCCCGACATCCC
CTGGCAGCTGCCCGGGGAGCAGCGGCTGTTCAAGGGCCTGACCTGGGGGCACATCCTGGTGATGGGCCGGGCGACCTACG
ACTCGATCGGCCGGCCGCTGCCCGGGCGCACCACGATCGTGCTCACCCGGTCCCCGGACTGGTCGGCCGAGGGGGTGCAT
GTCGCCCACGACCTGCCGGCGGCCCTCGCGCTCGCCGACACGCTCGAGGGCGAGGTGATGGTCGCCGGAGGCGGCCAGGT
GTACGCCGCCGCGCTGCCGGTCGCCGACGAGCAGGTGCTCAGCGAGGTGGACCTGGAGCCGCAGGGCGACGCGTTCTACC
CCGAGTTCGACCGCAGCGAGTGGCGCGAGACCGCCCGCGAGCAGCACGACGGGTACGACCGCGTGGTGCTTGTCCGGGCC
TGA

Upstream 100 bases:

>100_bases
CTGGACGCGTTCGACCTCGAGCACATCGCGGTCGAGGGCTACGACCCGCACCCCGGAATCAAGGCCCCCATCGCCGTATG
AGCCGGCAGGTGAGCGGCCG

Downstream 100 bases:

>100_bases
CAGGCTGGCGGGCATGGAGCTGCGCGTCTTCACCGAACCCCAGCAGGGAGCCACCTTCGACGACCTGGTGCGGGTCGCCC
AGGAGGCCGAACGGCTCGGC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTPGGKRVVLVAAVARNGVIGDGPDIPWQLPGEQRLFKGLTWGHILVMGRATYDSIGRPLPGRTTIVLTRSPDWSAEGVH
VAHDLPAALALADTLEGEVMVAGGGQVYAAALPVADEQVLSEVDLEPQGDAFYPEFDRSEWRETAREQHDGYDRVVLVRA

Sequences:

>Translated_160_residues
MTPGGKRVVLVAAVARNGVIGDGPDIPWQLPGEQRLFKGLTWGHILVMGRATYDSIGRPLPGRTTIVLTRSPDWSAEGVH
VAHDLPAALALADTLEGEVMVAGGGQVYAAALPVADEQVLSEVDLEPQGDAFYPEFDRSEWRETAREQHDGYDRVVLVRA
>Mature_159_residues
TPGGKRVVLVAAVARNGVIGDGPDIPWQLPGEQRLFKGLTWGHILVMGRATYDSIGRPLPGRTTIVLTRSPDWSAEGVHV
AHDLPAALALADTLEGEVMVAGGGQVYAAALPVADEQVLSEVDLEPQGDAFYPEFDRSEWRETAREQHDGYDRVVLVRA

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=144, Percent_Identity=38.1944444444444, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17281; Mature: 17149

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGGKRVVLVAAVARNGVIGDGPDIPWQLPGEQRLFKGLTWGHILVMGRATYDSIGRPL
CCCCCCEEEEEEEECCCCCCCCCCCCCEECCCHHHHHHCCCCEEEEEEECCCHHHHCCCC
PGRTTIVLTRSPDWSAEGVHVAHDLPAALALADTLEGEVMVAGGGQVYAAALPVADEQVL
CCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEECCCEEEEEECCCCHHHHH
SEVDLEPQGDAFYPEFDRSEWRETAREQHDGYDRVVLVRA
HHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
TPGGKRVVLVAAVARNGVIGDGPDIPWQLPGEQRLFKGLTWGHILVMGRATYDSIGRPL
CCCCCEEEEEEEECCCCCCCCCCCCCEECCCHHHHHHCCCCEEEEEEECCCHHHHCCCC
PGRTTIVLTRSPDWSAEGVHVAHDLPAALALADTLEGEVMVAGGGQVYAAALPVADEQVL
CCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEECCCEEEEEECCCCHHHHH
SEVDLEPQGDAFYPEFDRSEWRETAREQHDGYDRVVLVRA
HHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]