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The map label for this gene is mhpE [C]

Identifier: 119716380

GI number: 119716380

Start: 2298450

End: 2299478

Strand: Reverse

Name: mhpE [C]

Synonym: Noca_2149

Alternate gene names: 119716380

Gene position: 2299478-2298450 (Counterclockwise)

Preceding gene: 119716381

Following gene: 119716379

Centisome position: 46.12

GC content: 63.65

Gene sequence:

>1029_bases
GTGAACGAAACGCGTACGACTGTCTTTGTCCAGGACGTCACTCTCCGCGACGGCATGCACGCCATCCGACACCAGCTTGC
GCCCGGCGAGGTAGCCCAGATTGCCGCAGCCCTTGATGTCGCAGGGGTCGACGCCATCGAAATCTCGCATGGCGACGGTC
TCGCCGGAAGCAGCCTCAACTACGGTCCCGGAAGCCATACCGACTGGGAGTGGATCGAGGCTGCAGCTGCCAACATCCAA
CGCGCTCGGTTGACCACGCTCCTACTTCCGGGCATCGGGACGGTGGATGAATTGCGCAAGGCGCACGACCTCGGTGTTCG
CTCCGTTCGTGTCGCGACGCATTGCACGGAAGCCGACGTTTCGGCCCAGCACATCGAGACGGCGCGCGACCTCGGCATGG
ATGTCGCAGGATTTCTGATGATGAGCCACATGGCTGCGGCCAGCGAGCTGGCAGCCCAAGCGGCTCTCATGGAGTCCTAC
GGCGCCCATTGCGTCTACGTGACGGACTCGGGTGGCCGGCTGACCATGGACGCCGTGCGTGACCGCGTCCGGGCATACCG
TGACGTCTTGGATGCCACGACCGAGATCGGTATCCATGCCCATGAGAACTTGTCTCTGTCGGTCGCTAACAGCGTGGTTG
CGGTTGAAGCGGGCGTCACTCGGGTCGACGCCTCGCTCGCGGGACAAGGTGCAGGTGCGGGAAACTGCCCCATCGAGGCC
TTTGTCGCCGTGGCCAATATCCTCGGCTGGCAACATGGCTGCGACCTCTACCAACTGCAAGACGCTGCCGAGGACCTCGT
TCGCCCGCTCCAAGACCGGCCTGTACGCGTGGACCGGGAAACCTTGACGCTCGGCTACGCCGGCGTGTACTCCAGCTTCT
TGCGGCACGCCGAGAAGGCGGCTCAGACCTACGACCTCGACGTTCGAACCATCCTGACCGAGGTGGGGAATCGCCGGCTC
GTCGGAGGCCAAGAAGACATGATCGTCGACATCGCCATGGAACTGTCCGAGGTAGCGGCAGACCGTTGA

Upstream 100 bases:

>100_bases
ACTACCTGCCGGCATATGCAGGCAATCTCGACATCATGACCTCCGCAGCGTTGCGTGTTGCGGAAGCGATGGCAGCCCAG
AAACTCAGCGGAGCAGTGGC

Downstream 100 bases:

>100_bases
GGACGGTTGGCCGACTGCTCTATCGCGACGCTCTGCACCTCGTCAGGTAACAACTTCGCATTGGCCTCGCCAGCATTCCA
CTCAAAGGAATGGAGCCGTT

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 2; 4-hydroxy-2-keto-pentanoic acid aldolase 2; 4-hydroxy-2-oxopentanoate aldolase 2

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNETRTTVFVQDVTLRDGMHAIRHQLAPGEVAQIAAALDVAGVDAIEISHGDGLAGSSLNYGPGSHTDWEWIEAAAANIQ
RARLTTLLLPGIGTVDELRKAHDLGVRSVRVATHCTEADVSAQHIETARDLGMDVAGFLMMSHMAAASELAAQAALMESY
GAHCVYVTDSGGRLTMDAVRDRVRAYRDVLDATTEIGIHAHENLSLSVANSVVAVEAGVTRVDASLAGQGAGAGNCPIEA
FVAVANILGWQHGCDLYQLQDAAEDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAEKAAQTYDLDVRTILTEVGNRRL
VGGQEDMIVDIAMELSEVAADR

Sequences:

>Translated_342_residues
MNETRTTVFVQDVTLRDGMHAIRHQLAPGEVAQIAAALDVAGVDAIEISHGDGLAGSSLNYGPGSHTDWEWIEAAAANIQ
RARLTTLLLPGIGTVDELRKAHDLGVRSVRVATHCTEADVSAQHIETARDLGMDVAGFLMMSHMAAASELAAQAALMESY
GAHCVYVTDSGGRLTMDAVRDRVRAYRDVLDATTEIGIHAHENLSLSVANSVVAVEAGVTRVDASLAGQGAGAGNCPIEA
FVAVANILGWQHGCDLYQLQDAAEDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAEKAAQTYDLDVRTILTEVGNRRL
VGGQEDMIVDIAMELSEVAADR
>Mature_342_residues
MNETRTTVFVQDVTLRDGMHAIRHQLAPGEVAQIAAALDVAGVDAIEISHGDGLAGSSLNYGPGSHTDWEWIEAAAANIQ
RARLTTLLLPGIGTVDELRKAHDLGVRSVRVATHCTEADVSAQHIETARDLGMDVAGFLMMSHMAAASELAAQAALMESY
GAHCVYVTDSGGRLTMDAVRDRVRAYRDVLDATTEIGIHAHENLSLSVANSVVAVEAGVTRVDASLAGQGAGAGNCPIEA
FVAVANILGWQHGCDLYQLQDAAEDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAEKAAQTYDLDVRTILTEVGNRRL
VGGQEDMIVDIAMELSEVAADR

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=328, Percent_Identity=66.4634146341463, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA2_NOCSJ (A1SIM3)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_923345.1
- ProteinModelPortal:   A1SIM3
- SMR:   A1SIM3
- STRING:   A1SIM3
- GeneID:   4599209
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_2149
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   RRAYDIG
- ProtClustDB:   PRK08195
- BioCyc:   NSP35761:NOCA_2149-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36535; Mature: 36535

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 170-170 BINDING 199-199 BINDING 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETRTTVFVQDVTLRDGMHAIRHQLAPGEVAQIAAALDVAGVDAIEISHGDGLAGSSLN
CCCCEEEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
YGPGSHTDWEWIEAAAANIQRARLTTLLLPGIGTVDELRKAHDLGVRSVRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCC
SAQHIETARDLGMDVAGFLMMSHMAAASELAAQAALMESYGAHCVYVTDSGGRLTMDAVR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHH
DRVRAYRDVLDATTEIGIHAHENLSLSVANSVVAVEAGVTRVDASLAGQGAGAGNCPIEA
HHHHHHHHHHHHHHHCCEEECCCCEEEECCCEEEEECCCHHHHHHHCCCCCCCCCCHHHH
FVAVANILGWQHGCDLYQLQDAAEDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAEKA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCHHEEEHHHHHHHHHHHHHHHH
AQTYDLDVRTILTEVGNRRLVGGQEDMIVDIAMELSEVAADR
HHHCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNETRTTVFVQDVTLRDGMHAIRHQLAPGEVAQIAAALDVAGVDAIEISHGDGLAGSSLN
CCCCEEEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
YGPGSHTDWEWIEAAAANIQRARLTTLLLPGIGTVDELRKAHDLGVRSVRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCC
SAQHIETARDLGMDVAGFLMMSHMAAASELAAQAALMESYGAHCVYVTDSGGRLTMDAVR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHH
DRVRAYRDVLDATTEIGIHAHENLSLSVANSVVAVEAGVTRVDASLAGQGAGAGNCPIEA
HHHHHHHHHHHHHHHCCEEECCCCEEEECCCEEEEECCCHHHHHHHCCCCCCCCCCHHHH
FVAVANILGWQHGCDLYQLQDAAEDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAEKA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCHHEEEHHHHHHHHHHHHHHHH
AQTYDLDVRTILTEVGNRRLVGGQEDMIVDIAMELSEVAADR
HHHCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA