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The map label for this gene is thcD [H]

Identifier: 119716365

GI number: 119716365

Start: 2283410

End: 2284618

Strand: Reverse

Name: thcD [H]

Synonym: Noca_2134

Alternate gene names: 119716365

Gene position: 2284618-2283410 (Counterclockwise)

Preceding gene: 119716366

Following gene: 119716364

Centisome position: 45.82

GC content: 63.19

Gene sequence:

>1209_bases
GTGATGGTCGAGACCGAGGATCCGGTGGTCGTCCTCGGCGGCGGCATGGCAGGTGGTCGCGCTGCGGAGTTCCTCGCGAT
GCGAGGCCGCGGCACTCGCGTCGTCTTGGTGTCGGACGAAGATCACCGTCCGTACCAGCGGCCTCCGCTGTCCAAGGAGT
CGACTTGGGACGATCCGGTTCGAGAGGAGCTGTTCTTCAGAGATGAAGAGTTCTACGCATCCAAGGGAGTTCAACTGGCT
CTCGGAACACGGGTCGAAGGGTTGTTGCCGGACAGCGATGTCTTGATCACGAATCAGGGCGAGAAGATCGCATTCTCAAG
GCTGTTGATTGCCACCGGTTGCCGAGTGCGCCGACTACAAGTGCCCGGCTCCGATCTCAGAGGTGTTCATTACCTGAGGT
CGATTCCGGACGCGAAAGGCCTGTGGGACGCCGTCCATGGCGGCGGTCCTGTCGTGGTCGTCGGCGCGGGCTACGTGGGT
TGCGAGTTGGCAGCGGTCATGGCGGGACTCCAGGTCGACGTCACCGTCGTCGAGGCGGGCTCCGAGCCACTCGGGATCTC
CTTAGGGGCAGAGGTCGGAGCATGGGTTCGGTCCTTCCACGAACGGCGTGGCGTCAAGTTTGTGACAGGTCGGACCGTCG
TACACATGGACGGCGAAGACCGGGTCGGCAGCGTTCGCCTCGACGATGGGACGAGGTTGCCAGCCAGCACGGTCGTCGTT
GGGATCGGAGTGGTCCCCGATGTCGAGTGGCTCTCGGGATCCGGTGTGGAGATCGGCGACGGAATCCTCGTCAATGAACG
ATGCGAGACGAGCAGGCCTGGGGTGTTTGCTGCAGGTGACGCCGTGCGATGGACTCACCCGCAGTACGGCTCGATTCGGC
TGGAGCACGAAACCAACGCCCAAGCGCAGGCTGTCGTCGCTGCACGGAACATTCTCGGCGGGTCGACAGAGTACAACCAC
CTCCCCTACGTGTGGTCCGACCAGTACGGCGACACGTTGGAGTACGTCGGATACACACGTGGATGGAATGAGGCGGTCGT
CCAGACCGACGAGACGAGCTTCACAGCCGCTTACCTGACTGGTGGCGCTGTTGCGGCGGTTGCTGCATACGCACGCCCCG
ATGTAATCGCACTTGCGAAGCAGGTCCTCAGCGACGGACCTGTTGCTCGCGAACTCTGGCGGGCGGCGGCCAAGGACCTA
GGGGTCTGA

Upstream 100 bases:

>100_bases
CGACCGAGCCCGTCCCTGTGTACCGGGTCGAGGTCGAGGATGGGGACATCTTCCTCGTCGAAGGTGCCCCGGATCCGGCG
ACGGCGTCAGTGCGGGCCTC

Downstream 100 bases:

>100_bases
CGATGCTTGGTGACGGGCGGTGCGTTCTCGAGAAGAGGGCTGTCTGGTCACCCGCCGTGCCGACGAGCAGCGACGCTGAG
AGGAGAGGGTCATGAGTGAG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MMVETEDPVVVLGGGMAGGRAAEFLAMRGRGTRVVLVSDEDHRPYQRPPLSKESTWDDPVREELFFRDEEFYASKGVQLA
LGTRVEGLLPDSDVLITNQGEKIAFSRLLIATGCRVRRLQVPGSDLRGVHYLRSIPDAKGLWDAVHGGGPVVVVGAGYVG
CELAAVMAGLQVDVTVVEAGSEPLGISLGAEVGAWVRSFHERRGVKFVTGRTVVHMDGEDRVGSVRLDDGTRLPASTVVV
GIGVVPDVEWLSGSGVEIGDGILVNERCETSRPGVFAAGDAVRWTHPQYGSIRLEHETNAQAQAVVAARNILGGSTEYNH
LPYVWSDQYGDTLEYVGYTRGWNEAVVQTDETSFTAAYLTGGAVAAVAAYARPDVIALAKQVLSDGPVARELWRAAAKDL
GV

Sequences:

>Translated_402_residues
MMVETEDPVVVLGGGMAGGRAAEFLAMRGRGTRVVLVSDEDHRPYQRPPLSKESTWDDPVREELFFRDEEFYASKGVQLA
LGTRVEGLLPDSDVLITNQGEKIAFSRLLIATGCRVRRLQVPGSDLRGVHYLRSIPDAKGLWDAVHGGGPVVVVGAGYVG
CELAAVMAGLQVDVTVVEAGSEPLGISLGAEVGAWVRSFHERRGVKFVTGRTVVHMDGEDRVGSVRLDDGTRLPASTVVV
GIGVVPDVEWLSGSGVEIGDGILVNERCETSRPGVFAAGDAVRWTHPQYGSIRLEHETNAQAQAVVAARNILGGSTEYNH
LPYVWSDQYGDTLEYVGYTRGWNEAVVQTDETSFTAAYLTGGAVAAVAAYARPDVIALAKQVLSDGPVARELWRAAAKDL
GV
>Mature_402_residues
MMVETEDPVVVLGGGMAGGRAAEFLAMRGRGTRVVLVSDEDHRPYQRPPLSKESTWDDPVREELFFRDEEFYASKGVQLA
LGTRVEGLLPDSDVLITNQGEKIAFSRLLIATGCRVRRLQVPGSDLRGVHYLRSIPDAKGLWDAVHGGGPVVVVGAGYVG
CELAAVMAGLQVDVTVVEAGSEPLGISLGAEVGAWVRSFHERRGVKFVTGRTVVHMDGEDRVGSVRLDDGTRLPASTVVV
GIGVVPDVEWLSGSGVEIGDGILVNERCETSRPGVFAAGDAVRWTHPQYGSIRLEHETNAQAQAVVAARNILGGSTEYNH
LPYVWSDQYGDTLEYVGYTRGWNEAVVQTDETSFTAAYLTGGAVAAVAAYARPDVIALAKQVLSDGPVARELWRAAAKDL
GV

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=392, Percent_Identity=33.4183673469388, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI65787454, Length=392, Percent_Identity=33.4183673469388, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI21389617, Length=392, Percent_Identity=33.4183673469388, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=27.5964391691395, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=27.5964391691395, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1788892, Length=339, Percent_Identity=32.1533923303835, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789765, Length=316, Percent_Identity=24.3670886075949, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789065, Length=312, Percent_Identity=22.1153846153846, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=397, Percent_Identity=33.2493702770781, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24639257, Length=412, Percent_Identity=27.9126213592233, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI281359715, Length=412, Percent_Identity=27.9126213592233, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI281359713, Length=412, Percent_Identity=27.9126213592233, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24639250, Length=412, Percent_Identity=27.9126213592233, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI18543267, Length=412, Percent_Identity=27.9126213592233, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24639252, Length=412, Percent_Identity=27.9126213592233, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24585130, Length=401, Percent_Identity=25.6857855361596, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=26.6106442577031, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=26.6106442577031, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43054; Mature: 43054

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVETEDPVVVLGGGMAGGRAAEFLAMRGRGTRVVLVSDEDHRPYQRPPLSKESTWDDPV
CCCCCCCCEEEEECCCCCCHHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHH
REELFFRDEEFYASKGVQLALGTRVEGLLPDSDVLITNQGEKIAFSRLLIATGCRVRRLQ
HHHHHCCCCHHHHCCCCEEEECCCCCCCCCCCCEEEECCCCEEHHHHHHHHCCCEEEEEE
VPGSDLRGVHYLRSIPDAKGLWDAVHGGGPVVVVGAGYVGCELAAVMAGLQVDVTVVEAG
CCCCCCCHHHHHHHCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHCCCEEEEEEEECC
SEPLGISLGAEVGAWVRSFHERRGVKFVTGRTVVHMDGEDRVGSVRLDDGTRLPASTVVV
CCCCEEEECCHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEEEECCCCCCCHHHEEE
GIGVVPDVEWLSGSGVEIGDGILVNERCETSRPGVFAAGDAVRWTHPQYGSIRLEHETNA
EECCCCCCCCCCCCCCEECCCEEEECCCCCCCCCEEECCCEEEECCCCCCCEEEEECCCC
QAQAVVAARNILGGSTEYNHLPYVWSDQYGDTLEYVGYTRGWNEAVVQTDETSFTAAYLT
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEHHCCCCCCCCCEEEEECCCCEEEEEEC
GGAVAAVAAYARPDVIALAKQVLSDGPVARELWRAAAKDLGV
CCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMVETEDPVVVLGGGMAGGRAAEFLAMRGRGTRVVLVSDEDHRPYQRPPLSKESTWDDPV
CCCCCCCCEEEEECCCCCCHHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHH
REELFFRDEEFYASKGVQLALGTRVEGLLPDSDVLITNQGEKIAFSRLLIATGCRVRRLQ
HHHHHCCCCHHHHCCCCEEEECCCCCCCCCCCCEEEECCCCEEHHHHHHHHCCCEEEEEE
VPGSDLRGVHYLRSIPDAKGLWDAVHGGGPVVVVGAGYVGCELAAVMAGLQVDVTVVEAG
CCCCCCCHHHHHHHCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHCCCEEEEEEEECC
SEPLGISLGAEVGAWVRSFHERRGVKFVTGRTVVHMDGEDRVGSVRLDDGTRLPASTVVV
CCCCEEEECCHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEEEECCCCCCCHHHEEE
GIGVVPDVEWLSGSGVEIGDGILVNERCETSRPGVFAAGDAVRWTHPQYGSIRLEHETNA
EECCCCCCCCCCCCCCEECCCEEEECCCCCCCCCEEECCCEEEECCCCCCCEEEEECCCC
QAQAVVAARNILGGSTEYNHLPYVWSDQYGDTLEYVGYTRGWNEAVVQTDETSFTAAYLT
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEHHCCCCCCCCCEEEEECCCCEEEEEEC
GGAVAAVAAYARPDVIALAKQVLSDGPVARELWRAAAKDLGV
CCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]