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The map label for this gene is narK [H]

Identifier: 119714615

GI number: 119714615

Start: 371696

End: 373204

Strand: Direct

Name: narK [H]

Synonym: Noca_0349

Alternate gene names: 119714615

Gene position: 371696-373204 (Clockwise)

Preceding gene: 119714609

Following gene: 119714616

Centisome position: 7.45

GC content: 69.45

Gene sequence:

>1509_bases
ATGGCCCTCACTTCCTTGCACACCGACGCCGCCGAGGCGTCCCCCATCCGCCGCCGGACCGGCCGCTGGATCGACGACTG
GCGCCCGGAGGACCCGGACTTCTGGGCGAACGGCGGCCAGCAGGTCGCCCGTCGCAACCTGGTCTGGTCGGTCTTCGCCG
AGCACCTCGGATTCTCCGTGTGGCTGATCTGGAGCGTCAGCTCGGCGTTCCTGCTCGCCCAGGGCTTCACGTTCACCCCG
CAGCAGCTGTTCCTCCTCGTCGCGATTCCCAACCTGGTCGGCTCGCTGCTGCGCGTCCCCTACACCCTCGCCGTGCCGCG
GTTCGGCGGCCGCAACTGGACCATGGTGAGCGCCGGCCTGCTGCTGGTCCCGACGCTCGGCTTCGCCTACGCGGTGACCG
ATCCGAGCACGCCGTACTGGGTCTTCTGCCTGATCGCGGCGTCCGCCGGCTTCGGCGGCGGGAACTTCGCGAGCTCGATG
GCGAACATCAACTTCTTCTACCCGACCGCCAAGAAGGGGGCCGCCCTCGGCCTCAACGCCGCCGGCGGGAACCTCGGCGT
CTCGCTGATCCAGTTCTTCCTGCCGCTGATCGTCGGTGGCGCGGCGATCTTCGGCCTGGCCAAGGCCAGCCCCGGCGGCA
TCCACCTCGAGCGGGCCGCCTGGGTGTACGCCGGTCTCGCGATCGTGGCCACGGCGGCGGCCTACTTCTTCATGGACAAC
CTCGGGACCGCCAGGTCCTCGGCCAGGGAGCAGCTCGGCGTCCTCAAACACAAGCAGACCTGGGTGATGTCGTTCCTCTA
CATCGGCACGTTCGGCTCGTTCATCGGCTACTCCGCCGCGATGCCGCTGCTGATCAAGCTCAACTTCTGGGTGCCCGAGC
CGGCCCCGCTCGGCACCGGCATCTACTTCGCCTACTTCGCCTTCCTCGGCGCGCTGGTCGGCTCGATCACCCGCCCGCTC
GGTGGCTGGCTGGCCGACAGGTACGGCGGCGCCAGGGTCACCCTCGGCGCCTTCACCGGGATCATCCTCAGCACCCTCGG
CGTGCTGTGGACGCTGCTGCAGCTGACCCCGAACCCTGCCGCGGACCCGGCGATCGCCGCCGACAACAAGTCCTGGTTCC
CGTACTTCCTCGGGTTCTTCCTGCTCGTCTTCGCTGCCACCGGCATCGGCAACGGCTCGACGTACAAGATGATCCCCGCG
ATCTTCCGGACCGAGGCCGAGCGGGCGACCGCCGTGGGCACCGAGGAGCGCACCGCCGCGCTCCTCGCCGCCACCAAGCG
GGCCTCGGCCGCGATCGGCATCATCGGCGCGGTCGGCGCCATCGGCGGGTTTCTGATCCCGATCACGTTCAGCTCGCCGT
GGGTGAGCGACCCGATGGAGGCCACCAAGACCGCGTTCGTCATCTTCACGGTCTTCTACCTGGCCTGCGCGGCCACCACC
TGGGTGGTCTACCTGCGCAGGCCCGCCGAGCACAAGGCGCACCTCAGCTACGCCGGCGCCGGGATCTGA

Upstream 100 bases:

>100_bases
CCGTTGGCGGGGGGCGCGGTGTGAGGTCGTGAGGACAAGTTGACCTTGCCGGTACGGCGGCCTGGGCGACGCGAAACCTG
CGCTGCCGAGACTGACCGGC

Downstream 100 bases:

>100_bases
TGACCCAGACCCACTGCCCCTACTGCTCGCTCCAGTGCGGCATGCGCCTCGAGGGTGGCCGCCGGCTGGAGGTGCGGCCG
TGGCCGGAGTTCCCCGTCAA

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MALTSLHTDAAEASPIRRRTGRWIDDWRPEDPDFWANGGQQVARRNLVWSVFAEHLGFSVWLIWSVSSAFLLAQGFTFTP
QQLFLLVAIPNLVGSLLRVPYTLAVPRFGGRNWTMVSAGLLLVPTLGFAYAVTDPSTPYWVFCLIAASAGFGGGNFASSM
ANINFFYPTAKKGAALGLNAAGGNLGVSLIQFFLPLIVGGAAIFGLAKASPGGIHLERAAWVYAGLAIVATAAAYFFMDN
LGTARSSAREQLGVLKHKQTWVMSFLYIGTFGSFIGYSAAMPLLIKLNFWVPEPAPLGTGIYFAYFAFLGALVGSITRPL
GGWLADRYGGARVTLGAFTGIILSTLGVLWTLLQLTPNPAADPAIAADNKSWFPYFLGFFLLVFAATGIGNGSTYKMIPA
IFRTEAERATAVGTEERTAALLAATKRASAAIGIIGAVGAIGGFLIPITFSSPWVSDPMEATKTAFVIFTVFYLACAATT
WVVYLRRPAEHKAHLSYAGAGI

Sequences:

>Translated_502_residues
MALTSLHTDAAEASPIRRRTGRWIDDWRPEDPDFWANGGQQVARRNLVWSVFAEHLGFSVWLIWSVSSAFLLAQGFTFTP
QQLFLLVAIPNLVGSLLRVPYTLAVPRFGGRNWTMVSAGLLLVPTLGFAYAVTDPSTPYWVFCLIAASAGFGGGNFASSM
ANINFFYPTAKKGAALGLNAAGGNLGVSLIQFFLPLIVGGAAIFGLAKASPGGIHLERAAWVYAGLAIVATAAAYFFMDN
LGTARSSAREQLGVLKHKQTWVMSFLYIGTFGSFIGYSAAMPLLIKLNFWVPEPAPLGTGIYFAYFAFLGALVGSITRPL
GGWLADRYGGARVTLGAFTGIILSTLGVLWTLLQLTPNPAADPAIAADNKSWFPYFLGFFLLVFAATGIGNGSTYKMIPA
IFRTEAERATAVGTEERTAALLAATKRASAAIGIIGAVGAIGGFLIPITFSSPWVSDPMEATKTAFVIFTVFYLACAATT
WVVYLRRPAEHKAHLSYAGAGI
>Mature_501_residues
ALTSLHTDAAEASPIRRRTGRWIDDWRPEDPDFWANGGQQVARRNLVWSVFAEHLGFSVWLIWSVSSAFLLAQGFTFTPQ
QLFLLVAIPNLVGSLLRVPYTLAVPRFGGRNWTMVSAGLLLVPTLGFAYAVTDPSTPYWVFCLIAASAGFGGGNFASSMA
NINFFYPTAKKGAALGLNAAGGNLGVSLIQFFLPLIVGGAAIFGLAKASPGGIHLERAAWVYAGLAIVATAAAYFFMDNL
GTARSSAREQLGVLKHKQTWVMSFLYIGTFGSFIGYSAAMPLLIKLNFWVPEPAPLGTGIYFAYFAFLGALVGSITRPLG
GWLADRYGGARVTLGAFTGIILSTLGVLWTLLQLTPNPAADPAIAADNKSWFPYFLGFFLLVFAATGIGNGSTYKMIPAI
FRTEAERATAVGTEERTAALLAATKRASAAIGIIGAVGAIGGFLIPITFSSPWVSDPMEATKTAFVIFTVFYLACAATTW
VVYLRRPAEHKAHLSYAGAGI

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=493, Percent_Identity=43.2048681541582, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1787743, Length=485, Percent_Identity=40.2061855670103, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53609; Mature: 53477

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTSLHTDAAEASPIRRRTGRWIDDWRPEDPDFWANGGQQVARRNLVWSVFAEHLGFSV
CCCCCCCCCCHHCCHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCEE
WLIWSVSSAFLLAQGFTFTPQQLFLLVAIPNLVGSLLRVPYTLAVPRFGGRNWTMVSAGL
EEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCH
LLVPTLGFAYAVTDPSTPYWVFCLIAASAGFGGGNFASSMANINFFYPTAKKGAALGLNA
HHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCCEEEEEC
AGGNLGVSLIQFFLPLIVGGAAIFGLAKASPGGIHLERAAWVYAGLAIVATAAAYFFMDN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
LGTARSSAREQLGVLKHKQTWVMSFLYIGTFGSFIGYSAAMPLLIKLNFWVPEPAPLGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCH
IYFAYFAFLGALVGSITRPLGGWLADRYGGARVTLGAFTGIILSTLGVLWTLLQLTPNPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
ADPAIAADNKSWFPYFLGFFLLVFAATGIGNGSTYKMIPAIFRTEAERATAVGTEERTAA
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
LLAATKRASAAIGIIGAVGAIGGFLIPITFSSPWVSDPMEATKTAFVIFTVFYLACAATT
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
WVVYLRRPAEHKAHLSYAGAGI
HHHHHCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
ALTSLHTDAAEASPIRRRTGRWIDDWRPEDPDFWANGGQQVARRNLVWSVFAEHLGFSV
CCCCCCCCCHHCCHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCEE
WLIWSVSSAFLLAQGFTFTPQQLFLLVAIPNLVGSLLRVPYTLAVPRFGGRNWTMVSAGL
EEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCH
LLVPTLGFAYAVTDPSTPYWVFCLIAASAGFGGGNFASSMANINFFYPTAKKGAALGLNA
HHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCCEEEEEC
AGGNLGVSLIQFFLPLIVGGAAIFGLAKASPGGIHLERAAWVYAGLAIVATAAAYFFMDN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
LGTARSSAREQLGVLKHKQTWVMSFLYIGTFGSFIGYSAAMPLLIKLNFWVPEPAPLGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCH
IYFAYFAFLGALVGSITRPLGGWLADRYGGARVTLGAFTGIILSTLGVLWTLLQLTPNPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
ADPAIAADNKSWFPYFLGFFLLVFAATGIGNGSTYKMIPAIFRTEAERATAVGTEERTAA
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
LLAATKRASAAIGIIGAVGAIGGFLIPITFSSPWVSDPMEATKTAFVIFTVFYLACAATT
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
WVVYLRRPAEHKAHLSYAGAGI
HHHHHCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]