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The map label for this gene is nasC [H]

Identifier: 119714616

GI number: 119714616

Start: 373204

End: 375246

Strand: Direct

Name: nasC [H]

Synonym: Noca_0350

Alternate gene names: 119714616

Gene position: 373204-375246 (Clockwise)

Preceding gene: 119714615

Following gene: 119714617

Centisome position: 7.49

GC content: 75.18

Gene sequence:

>2043_bases
ATGACCCAGACCCACTGCCCCTACTGCTCGCTCCAGTGCGGCATGCGCCTCGAGGGTGGCCGCCGGCTGGAGGTGCGGCC
GTGGCCGGAGTTCCCCGTCAACGAGGGCGCGCTGTGTCGCAAGGGCTGGACGGCGGCCGGCCTGCGCGGCCACCGCGAGC
GGCTCACGACGCCGCTGATCCGGGACCGGGCCACGGGTGAGCTCCGCGCGGCCGGCTGGGACGAGGCACTCGACCTGGTC
GCCAGCCGGCTGTGCGGCCTGCGCGACGAGCACGGCCCGGACTCCGTCGCGGTGTTCGGGGGCGGCGGGTTGACCAACGA
GAAGGCCTACCAGCTGGGCAAGCTGGCCCGGGTCGCGCTCGGCACCAGCCAGGTCGACTACAACGGCCGGTGGTGCATGT
CGTCGGCGGCCTCGGCCGGCAATCGGGCTCTCGGGATCGACCGCGGCCTGCCGTTCCCGCTCGCGGACGTCGAGCACACC
GACGTGCTGGTGCTCGTGGGATCCAACCTCGCGGAGACCATGCCCCCCGCCGCGCGCCACCTCGACCGGCTCCGCGAGAA
CGGCGGCCGCGTGGTCGTCGTCGACCCCCGGCGTACCCCCACCGCCGAGCGCGCCGACCTGTTCCTCCAGCCGGTCCCGG
GCACCGACCTGCCGCTCGCGCTCGGCCTGCTGCACCTGCTCGACGCTGCGGGTGCGGTCGCCGAGGAGTACGTCGCCGCG
CGGACGACCGGCTGGGAGGAGGTCCGCCGCTCGCTCGCCGGATGGTGGCCCGAGCGGGTCGAACGGGTCGCCGGCGTCCA
GGCGTCGCAACTGCGGGAGTGCGCGGACCTGCTGGCCGGTGCCGAGCGGGTGATGGTCCTGACCGCCCGCGGCGCCGAGC
AGCACGCCCAGGGCACCGACACCGTGCTCGCCTGGATCGACCTGGCGCTGGCGCTCGGCATGCCCGGCAAGCAGTACGCC
GGCTACGGCTGCCTCACCGGGCAGGGCAACGGACAGGGCGGCCGGGAGCACGGGCAGAAGGCCGACCAGCTGCCGGGCTA
CCGGATGATCGACGACCCCTTGGCGCGCGACCACGTGGCCGAGGTCTGGGGAGTCTCCACGGACTCGCTGCCCGGGCCGG
GCCGCTCGGCGTACGAGCTGCTCGACTCCCTCGGCACCGGCGGCGGTCCGCGGGCCTTGCTGGTGGTCGGCAGCAACATC
GTGGTCTCGGCCCCGAACGCCACCCACGTGGCGTCCCGGCTCGCCGCGCTCGACCTGCTCGTGGTGGCCGACCTCGTGCT
GAGCGAGACCGCGGCGCAGGCCGACGTCGTCCTGCCGGTCGCGCAGTGGGCGGAGGAGACCGGCACGATGACCAACCTCG
AAGGGCGGGTGATCCTGCGTGAGCGGGCCGTCGCCCCGCCCGAGGGGGTGCGCACCGACCTCGAGGTGATCGCCGGTCTC
GCCGAGCGGCTCGGCTCGCCGGTGCCGTTCGCCGTCGATCCCGAGGAGGTCTTCGCCGAGCTCGGCCGGGCCTCGAGCGG
CGGGCGCGCCGACTACTCCGGCATCGGCTACGACCGGATCCGCGACGAGCACGGGGTGTTCTGGCCGTGCCCGTCGCCCG
ACCACCCGGGGACCCCGCGGCTGTTCGCCGAGTCCTTCCCCACGGCCGACGGCCGCGCCCGCTTCGTCGCGGTCGAGCAC
CGCGGTCCGGCCGAGCCGACCGACCCGTCGTACCCGATCCACCTGACGACCGGCCGGGTGCTCGCGCAGTACCAGTCCGG
CGCGCAGACCCGGCGGGTCCGCGAGCTGCCCGACGAGGGCCCGTTCGTCGAGATGCACCCGATGCTCGCGGCGCGGATCG
GCGCGCACGACGGCCAGCAGGTGGTCGTCACCACGCGCCGCGGCGAGCTCAGGGTGCCCGCGCGGGTGACGGCCACGATC
CGTCCGGACACCGTGTTCGTGCCCTTCCACTGGGTCGGCGCCAACCGGCTCACCCACGACGCCCTCGACCCGGCCAGCCG
GATGCCGGAGTTCAAGGTGTGCGCCGCGGCGGTGCGCGCATGA

Upstream 100 bases:

>100_bases
CGGTCTTCTACCTGGCCTGCGCGGCCACCACCTGGGTGGTCTACCTGCGCAGGCCCGCCGAGCACAAGGCGCACCTCAGC
TACGCCGGCGCCGGGATCTG

Downstream 100 bases:

>100_bases
GCCGCGCACCGGCCGGCGGTGGGTCAGCAACCGTTGGGGGCGCTCATTCGGTTACCTGCTCACCGCTGGCCGGGGGTGGC
GGGGGTGGGCGGTGTGTCCG

Product: assimilatory nitrate reductase (NADH) subunit alpha apoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MTQTHCPYCSLQCGMRLEGGRRLEVRPWPEFPVNEGALCRKGWTAAGLRGHRERLTTPLIRDRATGELRAAGWDEALDLV
ASRLCGLRDEHGPDSVAVFGGGGLTNEKAYQLGKLARVALGTSQVDYNGRWCMSSAASAGNRALGIDRGLPFPLADVEHT
DVLVLVGSNLAETMPPAARHLDRLRENGGRVVVVDPRRTPTAERADLFLQPVPGTDLPLALGLLHLLDAAGAVAEEYVAA
RTTGWEEVRRSLAGWWPERVERVAGVQASQLRECADLLAGAERVMVLTARGAEQHAQGTDTVLAWIDLALALGMPGKQYA
GYGCLTGQGNGQGGREHGQKADQLPGYRMIDDPLARDHVAEVWGVSTDSLPGPGRSAYELLDSLGTGGGPRALLVVGSNI
VVSAPNATHVASRLAALDLLVVADLVLSETAAQADVVLPVAQWAEETGTMTNLEGRVILRERAVAPPEGVRTDLEVIAGL
AERLGSPVPFAVDPEEVFAELGRASSGGRADYSGIGYDRIRDEHGVFWPCPSPDHPGTPRLFAESFPTADGRARFVAVEH
RGPAEPTDPSYPIHLTTGRVLAQYQSGAQTRRVRELPDEGPFVEMHPMLAARIGAHDGQQVVVTTRRGELRVPARVTATI
RPDTVFVPFHWVGANRLTHDALDPASRMPEFKVCAAAVRA

Sequences:

>Translated_680_residues
MTQTHCPYCSLQCGMRLEGGRRLEVRPWPEFPVNEGALCRKGWTAAGLRGHRERLTTPLIRDRATGELRAAGWDEALDLV
ASRLCGLRDEHGPDSVAVFGGGGLTNEKAYQLGKLARVALGTSQVDYNGRWCMSSAASAGNRALGIDRGLPFPLADVEHT
DVLVLVGSNLAETMPPAARHLDRLRENGGRVVVVDPRRTPTAERADLFLQPVPGTDLPLALGLLHLLDAAGAVAEEYVAA
RTTGWEEVRRSLAGWWPERVERVAGVQASQLRECADLLAGAERVMVLTARGAEQHAQGTDTVLAWIDLALALGMPGKQYA
GYGCLTGQGNGQGGREHGQKADQLPGYRMIDDPLARDHVAEVWGVSTDSLPGPGRSAYELLDSLGTGGGPRALLVVGSNI
VVSAPNATHVASRLAALDLLVVADLVLSETAAQADVVLPVAQWAEETGTMTNLEGRVILRERAVAPPEGVRTDLEVIAGL
AERLGSPVPFAVDPEEVFAELGRASSGGRADYSGIGYDRIRDEHGVFWPCPSPDHPGTPRLFAESFPTADGRARFVAVEH
RGPAEPTDPSYPIHLTTGRVLAQYQSGAQTRRVRELPDEGPFVEMHPMLAARIGAHDGQQVVVTTRRGELRVPARVTATI
RPDTVFVPFHWVGANRLTHDALDPASRMPEFKVCAAAVRA
>Mature_679_residues
TQTHCPYCSLQCGMRLEGGRRLEVRPWPEFPVNEGALCRKGWTAAGLRGHRERLTTPLIRDRATGELRAAGWDEALDLVA
SRLCGLRDEHGPDSVAVFGGGGLTNEKAYQLGKLARVALGTSQVDYNGRWCMSSAASAGNRALGIDRGLPFPLADVEHTD
VLVLVGSNLAETMPPAARHLDRLRENGGRVVVVDPRRTPTAERADLFLQPVPGTDLPLALGLLHLLDAAGAVAEEYVAAR
TTGWEEVRRSLAGWWPERVERVAGVQASQLRECADLLAGAERVMVLTARGAEQHAQGTDTVLAWIDLALALGMPGKQYAG
YGCLTGQGNGQGGREHGQKADQLPGYRMIDDPLARDHVAEVWGVSTDSLPGPGRSAYELLDSLGTGGGPRALLVVGSNIV
VSAPNATHVASRLAALDLLVVADLVLSETAAQADVVLPVAQWAEETGTMTNLEGRVILRERAVAPPEGVRTDLEVIAGLA
ERLGSPVPFAVDPEEVFAELGRASSGGRADYSGIGYDRIRDEHGVFWPCPSPDHPGTPRLFAESFPTADGRARFVAVEHR
GPAEPTDPSYPIHLTTGRVLAQYQSGAQTRRVRELPDEGPFVEMHPMLAARIGAHDGQQVVVTTRRGELRVPARVTATIR
PDTVFVPFHWVGANRLTHDALDPASRMPEFKVCAAAVRA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=689, Percent_Identity=30.33381712627, Blast_Score=339, Evalue=3e-94,
Organism=Escherichia coli, GI1788534, Length=786, Percent_Identity=24.8091603053435, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI1787778, Length=681, Percent_Identity=25.5506607929515, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI3868720, Length=620, Percent_Identity=23.5483870967742, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI3868719, Length=617, Percent_Identity=22.5283630470016, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI87081994, Length=685, Percent_Identity=23.5036496350365, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI145693196, Length=726, Percent_Identity=23.0027548209366, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081797, Length=684, Percent_Identity=22.2222222222222, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI171474008, Length=743, Percent_Identity=21.9380888290713, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787870, Length=728, Percent_Identity=21.8406593406593, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 72941; Mature: 72810

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTHCPYCSLQCGMRLEGGRRLEVRPWPEFPVNEGALCRKGWTAAGLRGHRERLTTPLI
CCCCCCCCEECCCCEEECCCCEEEECCCCCCCCCCCCEECCCCCHHCCCHHHHHHCCHHH
RDRATGELRAAGWDEALDLVASRLCGLRDEHGPDSVAVFGGGGLTNEKAYQLGKLARVAL
HCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
GTSQVDYNGRWCMSSAASAGNRALGIDRGLPFPLADVEHTDVLVLVGSNLAETMPPAARH
CCCCCCCCCCEEHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCHHHHCCHHHHH
LDRLRENGGRVVVVDPRRTPTAERADLFLQPVPGTDLPLALGLLHLLDAAGAVAEEYVAA
HHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RTTGWEEVRRSLAGWWPERVERVAGVQASQLRECADLLAGAERVMVLTARGAEQHAQGTD
HCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHCCCHH
TVLAWIDLALALGMPGKQYAGYGCLTGQGNGQGGREHGQKADQLPGYRMIDDPLARDHVA
HHHHHHHHHHHHCCCCCHHCCCEEEECCCCCCCCHHHCCHHHHCCCCEECCCCHHHHHHH
EVWGVSTDSLPGPGRSAYELLDSLGTGGGPRALLVVGSNIVVSAPNATHVASRLAALDLL
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHH
VVADLVLSETAAQADVVLPVAQWAEETGTMTNLEGRVILRERAVAPPEGVRTDLEVIAGL
HHHHHHHHHHHHHCCEEEHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCHHHHHHHHHH
AERLGSPVPFAVDPEEVFAELGRASSGGRADYSGIGYDRIRDEHGVFWPCPSPDHPGTPR
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCC
LFAESFPTADGRARFVAVEHRGPAEPTDPSYPIHLTTGRVLAQYQSGAQTRRVRELPDEG
HHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHCCHHHHHHHHCCCCC
PFVEMHPMLAARIGAHDGQQVVVTTRRGELRVPARVTATIRPDTVFVPFHWVGANRLTHD
CEEEECHHHHHHHCCCCCCEEEEEECCCCEECCEEEEEEECCCEEEEEEEEECCCCCCHH
ALDPASRMPEFKVCAAAVRA
HCCHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
TQTHCPYCSLQCGMRLEGGRRLEVRPWPEFPVNEGALCRKGWTAAGLRGHRERLTTPLI
CCCCCCCEECCCCEEECCCCEEEECCCCCCCCCCCCEECCCCCHHCCCHHHHHHCCHHH
RDRATGELRAAGWDEALDLVASRLCGLRDEHGPDSVAVFGGGGLTNEKAYQLGKLARVAL
HCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
GTSQVDYNGRWCMSSAASAGNRALGIDRGLPFPLADVEHTDVLVLVGSNLAETMPPAARH
CCCCCCCCCCEEHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCHHHHCCHHHHH
LDRLRENGGRVVVVDPRRTPTAERADLFLQPVPGTDLPLALGLLHLLDAAGAVAEEYVAA
HHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RTTGWEEVRRSLAGWWPERVERVAGVQASQLRECADLLAGAERVMVLTARGAEQHAQGTD
HCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHCCCHH
TVLAWIDLALALGMPGKQYAGYGCLTGQGNGQGGREHGQKADQLPGYRMIDDPLARDHVA
HHHHHHHHHHHHCCCCCHHCCCEEEECCCCCCCCHHHCCHHHHCCCCEECCCCHHHHHHH
EVWGVSTDSLPGPGRSAYELLDSLGTGGGPRALLVVGSNIVVSAPNATHVASRLAALDLL
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHH
VVADLVLSETAAQADVVLPVAQWAEETGTMTNLEGRVILRERAVAPPEGVRTDLEVIAGL
HHHHHHHHHHHHHCCEEEHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCHHHHHHHHHH
AERLGSPVPFAVDPEEVFAELGRASSGGRADYSGIGYDRIRDEHGVFWPCPSPDHPGTPR
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCC
LFAESFPTADGRARFVAVEHRGPAEPTDPSYPIHLTTGRVLAQYQSGAQTRRVRELPDEG
HHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHCCHHHHHHHHCCCCC
PFVEMHPMLAARIGAHDGQQVVVTTRRGELRVPARVTATIRPDTVFVPFHWVGANRLTHD
CEEEECHHHHHHHCCCCCCEEEEEECCCCEECCEEEEEEECCCEEEEEEEEECCCCCCHH
ALDPASRMPEFKVCAAAVRA
HCCHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]