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The map label for this gene is glgX [H]

Identifier: 119714536

GI number: 119714536

Start: 289765

End: 291966

Strand: Reverse

Name: glgX [H]

Synonym: Noca_0269

Alternate gene names: 119714536

Gene position: 291966-289765 (Counterclockwise)

Preceding gene: 119714541

Following gene: 119714535

Centisome position: 5.86

GC content: 68.76

Gene sequence:

>2202_bases
GTGCAGGTCTGGCCGGGACGGGCGTACCCGCTCGGGGCGACGTTCGACGGGAGCGGGACGAACTTCGCCCTCTTCAGCGA
GGTCGCCGACCGCGTCGAGCTGTGCCTCTTCGACGCAGAGGGCTCGGAGGGATACCGGGAGACCCGCGTCGAGCTCACCG
AGGTCGACGCCTACGTGTGGCACTGCTACCTGCCCGCCGTACAGCCGGGCCAGCGCTACGGCTACCGGGTACACGGGCCC
TGGGACCCGGAGAACGGACAGCGCTGCAACCCGAACAAGCTGCTGCTCGACCCGTACGCCAAGGCCACCAGCGGTGAGAT
CGACTGGGACCAGAGCCTGTTCTCCTACAACTTCGGCGACCCCGAGGACAGCGACGCGCGCAACGACGAGGACTCCGCCC
CGCACATGACCCACGGGGTCGTGATCAACCCCTTCTTCGACTGGGAGGGCGACCGCCGGCTCGACTACGACTACAACGAG
TCGATCATCTACGAGGCACACGTCAAGGGCCTCACGCAGCTCCACCCCGACATCCCCGAGGAGCTGCGGGGGACGTACGC
CGCCCTCGGGCACCCGGCGATCACCGAGCACCTGACCAAGCTCGGCGTGACCGCGATCGAGCTGATGCCGGTGCACCAGT
TCATCCAGGACAGCACGCTGCTGGACAAGGGCCTGCGCAACTACTGGGGCTACAACACCCTGGGCTTCTTCGCTCCGCAC
GCCGACTATGCCGCCACCGGGCAGCCCGGCCAGCAGGTGCAGGAGTTCAAGTCGATGGTCAAGGCGATGCACGCCGCGGG
CATCGAGGTGATCCTCGACGTGGTCTACAACCACACCGCGGAGGGCAACCACCTCGGCCCCACGCTGAGCTTCAAGGGCA
TCGACAACGCGGCGTACTACCGGCTGGTCGAGGACGACCAGCGCTACTACATGGACTACACCGGCACCGGCAACAGCCTC
AACGTGCGGCACCCGCACTCGCTGCAGCTGCTGATGGACTCGCTGCGGTACTGGGTCACCGAGATGCACGTCGACGGCTT
CCGCTTCGACCTCGCCTCGACGTTGGCTCGCGAGTTCTACGACGTCGACCGGCTCTCGACGTTCTTCGAGCTCGTGCAGC
AGGACCCAGTGGTCAGCCAGGTCAAGCTGATCGCCGAGCCGTGGGACATCGGCCCCGGCGGCTACCAGGTCGGCGGCTTC
CCGCCGCAGTGGACGGAGTGGAACGGCAAGTATCGCGACACCGTCCGCGACTTCTGGCGCGGCGAGCCGTCGCTGGGCGA
GTTCGCCTCGCGGCTCGCCGGGTCGGCCGACCTCTACGAGCACTCCGGCCGCCGGCCGGTGGCCAGCATCAACTTCGTCA
CCGCCCACGACGGCTTCACCCTGCGCGACCTGGTCTCCTACGACGAGAAGCACAACGAGGCGAACGGCGAGGACAACAAC
GACGGCGAGAGCCACAACCGCTCGTGGAACCACGGTGTCGAGGGCCCCACCGACGACCCCGAGATCCTCGAGTTCCGCGC
CCGGGAGCAGCGCAACTTCATCGCGACGCTACTGCTGAGCCAGGGCGTGCCGATGCTGCTCCACGGCGACGAGATGGGCC
GCACCCAGCAGGGCAACAACAACACCTACGCCCAGGACTCCGAGCTGAGCTGGGTGCACTGGGACGAGGCCGACAAGCCG
CTGGTCGAGTTCACCGCGGCGCTGTCCCGGCTGCGGCGCGCCCATCCCACGTTCCGGCGCAAGCGGTTCTTCACCGGTGG
CACCGTCCGGGTCCCCGATGGGGGCGACGGCGACCGGCTCAACGACATCGTCTGGCTGCACCTCGACGGCCGGCCGATGG
AGGACAACGACTGGACCCAGTCGGATTCCGGGGCAGGGGCACAGGCCCTCGGCATGTACCTCAACGGGCACGGGATCGCC
GGCCGTGACGAACGCGGCGGGCCGATCGTCGACAACCACTTCCTGCTCTACTTCAACGCCGACGGGCCGGCCGAGGTGAC
CCTCCCCCCGGACGAGTACGCCGACGCCTGGGACGTGGTCGTCGACACCGGCGGCCTGGTGGACGACCAGACCGCGTTGG
CCGCCGGGTCGACCTGCTCGATGGCGTCGCGCAGCCTGATCGTGCTCCGCGAGCACCGCGAGCCGGAGGCCGAGGTCGAC
CACTCGGTGGCAGCGTCGGTCGCCCAGGCCCGCCGGGGCTGA

Upstream 100 bases:

>100_bases
TACGGCGCTCCTCGGGTTGACGGACCAGACCGAGGGGTGTGCTTTGATCGTTCCAACCGCCGCTGGGGTGGGACAGCCGG
AGGAGCAGGTGGAGGGTGCC

Downstream 100 bases:

>100_bases
GGCGATGCGCGCGCCGGTCAGCACCTACCGTCTGCAGATCACGCCCGAGTTCGACCTGTTCGAGGCGGCCCGCCGGTTGC
CGTACCTGCGCGCCCTGGGC

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MQVWPGRAYPLGATFDGSGTNFALFSEVADRVELCLFDAEGSEGYRETRVELTEVDAYVWHCYLPAVQPGQRYGYRVHGP
WDPENGQRCNPNKLLLDPYAKATSGEIDWDQSLFSYNFGDPEDSDARNDEDSAPHMTHGVVINPFFDWEGDRRLDYDYNE
SIIYEAHVKGLTQLHPDIPEELRGTYAALGHPAITEHLTKLGVTAIELMPVHQFIQDSTLLDKGLRNYWGYNTLGFFAPH
ADYAATGQPGQQVQEFKSMVKAMHAAGIEVILDVVYNHTAEGNHLGPTLSFKGIDNAAYYRLVEDDQRYYMDYTGTGNSL
NVRHPHSLQLLMDSLRYWVTEMHVDGFRFDLASTLAREFYDVDRLSTFFELVQQDPVVSQVKLIAEPWDIGPGGYQVGGF
PPQWTEWNGKYRDTVRDFWRGEPSLGEFASRLAGSADLYEHSGRRPVASINFVTAHDGFTLRDLVSYDEKHNEANGEDNN
DGESHNRSWNHGVEGPTDDPEILEFRAREQRNFIATLLLSQGVPMLLHGDEMGRTQQGNNNTYAQDSELSWVHWDEADKP
LVEFTAALSRLRRAHPTFRRKRFFTGGTVRVPDGGDGDRLNDIVWLHLDGRPMEDNDWTQSDSGAGAQALGMYLNGHGIA
GRDERGGPIVDNHFLLYFNADGPAEVTLPPDEYADAWDVVVDTGGLVDDQTALAAGSTCSMASRSLIVLREHREPEAEVD
HSVAASVAQARRG

Sequences:

>Translated_733_residues
MQVWPGRAYPLGATFDGSGTNFALFSEVADRVELCLFDAEGSEGYRETRVELTEVDAYVWHCYLPAVQPGQRYGYRVHGP
WDPENGQRCNPNKLLLDPYAKATSGEIDWDQSLFSYNFGDPEDSDARNDEDSAPHMTHGVVINPFFDWEGDRRLDYDYNE
SIIYEAHVKGLTQLHPDIPEELRGTYAALGHPAITEHLTKLGVTAIELMPVHQFIQDSTLLDKGLRNYWGYNTLGFFAPH
ADYAATGQPGQQVQEFKSMVKAMHAAGIEVILDVVYNHTAEGNHLGPTLSFKGIDNAAYYRLVEDDQRYYMDYTGTGNSL
NVRHPHSLQLLMDSLRYWVTEMHVDGFRFDLASTLAREFYDVDRLSTFFELVQQDPVVSQVKLIAEPWDIGPGGYQVGGF
PPQWTEWNGKYRDTVRDFWRGEPSLGEFASRLAGSADLYEHSGRRPVASINFVTAHDGFTLRDLVSYDEKHNEANGEDNN
DGESHNRSWNHGVEGPTDDPEILEFRAREQRNFIATLLLSQGVPMLLHGDEMGRTQQGNNNTYAQDSELSWVHWDEADKP
LVEFTAALSRLRRAHPTFRRKRFFTGGTVRVPDGGDGDRLNDIVWLHLDGRPMEDNDWTQSDSGAGAQALGMYLNGHGIA
GRDERGGPIVDNHFLLYFNADGPAEVTLPPDEYADAWDVVVDTGGLVDDQTALAAGSTCSMASRSLIVLREHREPEAEVD
HSVAASVAQARRG
>Mature_733_residues
MQVWPGRAYPLGATFDGSGTNFALFSEVADRVELCLFDAEGSEGYRETRVELTEVDAYVWHCYLPAVQPGQRYGYRVHGP
WDPENGQRCNPNKLLLDPYAKATSGEIDWDQSLFSYNFGDPEDSDARNDEDSAPHMTHGVVINPFFDWEGDRRLDYDYNE
SIIYEAHVKGLTQLHPDIPEELRGTYAALGHPAITEHLTKLGVTAIELMPVHQFIQDSTLLDKGLRNYWGYNTLGFFAPH
ADYAATGQPGQQVQEFKSMVKAMHAAGIEVILDVVYNHTAEGNHLGPTLSFKGIDNAAYYRLVEDDQRYYMDYTGTGNSL
NVRHPHSLQLLMDSLRYWVTEMHVDGFRFDLASTLAREFYDVDRLSTFFELVQQDPVVSQVKLIAEPWDIGPGGYQVGGF
PPQWTEWNGKYRDTVRDFWRGEPSLGEFASRLAGSADLYEHSGRRPVASINFVTAHDGFTLRDLVSYDEKHNEANGEDNN
DGESHNRSWNHGVEGPTDDPEILEFRAREQRNFIATLLLSQGVPMLLHGDEMGRTQQGNNNTYAQDSELSWVHWDEADKP
LVEFTAALSRLRRAHPTFRRKRFFTGGTVRVPDGGDGDRLNDIVWLHLDGRPMEDNDWTQSDSGAGAQALGMYLNGHGIA
GRDERGGPIVDNHFLLYFNADGPAEVTLPPDEYADAWDVVVDTGGLVDDQTALAAGSTCSMASRSLIVLREHREPEAEVD
HSVAASVAQARRG

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=618, Percent_Identity=48.5436893203884, Blast_Score=563, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 82247; Mature: 82247

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVWPGRAYPLGATFDGSGTNFALFSEVADRVELCLFDAEGSEGYRETRVELTEVDAYVW
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHEEEEEEECCCCCCHHHHHCCHHHHCEEEE
HCYLPAVQPGQRYGYRVHGPWDPENGQRCNPNKLLLDPYAKATSGEIDWDQSLFSYNFGD
EEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCC
PEDSDARNDEDSAPHMTHGVVINPFFDWEGDRRLDYDYNESIIYEAHVKGLTQLHPDIPE
CCCCCCCCCCCCCCCEECCEEEEEEECCCCCCEECCCCCCCEEEEHHHHHHHHHCCCCCH
ELRGTYAALGHPAITEHLTKLGVTAIELMPVHQFIQDSTLLDKGLRNYWGYNTLGFFAPH
HHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
ADYAATGQPGQQVQEFKSMVKAMHAAGIEVILDVVYNHTAEGNHLGPTLSFKGIDNAAYY
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEE
RLVEDDQRYYMDYTGTGNSLNVRHPHSLQLLMDSLRYWVTEMHVDGFRFDLASTLAREFY
EEEECCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DVDRLSTFFELVQQDPVVSQVKLIAEPWDIGPGGYQVGGFPPQWTEWNGKYRDTVRDFWR
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHC
GEPSLGEFASRLAGSADLYEHSGRRPVASINFVTAHDGFTLRDLVSYDEKHNEANGEDNN
CCCCHHHHHHHHCCCHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
DGESHNRSWNHGVEGPTDDPEILEFRAREQRNFIATLLLSQGVPMLLHGDEMGRTQQGNN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
NTYAQDSELSWVHWDEADKPLVEFTAALSRLRRAHPTFRRKRFFTGGTVRVPDGGDGDRL
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHEEECCCEEECCCCCCCCCC
NDIVWLHLDGRPMEDNDWTQSDSGAGAQALGMYLNGHGIAGRDERGGPIVDNHFLLYFNA
CCEEEEEECCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCEECCCEEEEECC
DGPAEVTLPPDEYADAWDVVVDTGGLVDDQTALAAGSTCSMASRSLIVLREHREPEAEVD
CCCEEEEECCHHHCCCEEEEEECCCCCCCCHHHHCCCCHHHCCCEEEEEECCCCCCHHHH
HSVAASVAQARRG
HHHHHHHHHHHCC
>Mature Secondary Structure
MQVWPGRAYPLGATFDGSGTNFALFSEVADRVELCLFDAEGSEGYRETRVELTEVDAYVW
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHEEEEEEECCCCCCHHHHHCCHHHHCEEEE
HCYLPAVQPGQRYGYRVHGPWDPENGQRCNPNKLLLDPYAKATSGEIDWDQSLFSYNFGD
EEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCC
PEDSDARNDEDSAPHMTHGVVINPFFDWEGDRRLDYDYNESIIYEAHVKGLTQLHPDIPE
CCCCCCCCCCCCCCCEECCEEEEEEECCCCCCEECCCCCCCEEEEHHHHHHHHHCCCCCH
ELRGTYAALGHPAITEHLTKLGVTAIELMPVHQFIQDSTLLDKGLRNYWGYNTLGFFAPH
HHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
ADYAATGQPGQQVQEFKSMVKAMHAAGIEVILDVVYNHTAEGNHLGPTLSFKGIDNAAYY
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEE
RLVEDDQRYYMDYTGTGNSLNVRHPHSLQLLMDSLRYWVTEMHVDGFRFDLASTLAREFY
EEEECCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DVDRLSTFFELVQQDPVVSQVKLIAEPWDIGPGGYQVGGFPPQWTEWNGKYRDTVRDFWR
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHC
GEPSLGEFASRLAGSADLYEHSGRRPVASINFVTAHDGFTLRDLVSYDEKHNEANGEDNN
CCCCHHHHHHHHCCCHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
DGESHNRSWNHGVEGPTDDPEILEFRAREQRNFIATLLLSQGVPMLLHGDEMGRTQQGNN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
NTYAQDSELSWVHWDEADKPLVEFTAALSRLRRAHPTFRRKRFFTGGTVRVPDGGDGDRL
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHEEECCCEEECCCCCCCCCC
NDIVWLHLDGRPMEDNDWTQSDSGAGAQALGMYLNGHGIAGRDERGGPIVDNHFLLYFNA
CCEEEEEECCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCEECCCEEEEECC
DGPAEVTLPPDEYADAWDVVVDTGGLVDDQTALAAGSTCSMASRSLIVLREHREPEAEVD
CCCEEEEECCHHHCCCEEEEEECCCCCCCCHHHHCCCCHHHCCCEEEEEECCCCCCHHHH
HSVAASVAQARRG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]