Click here to switch to the map view.

The map label for this gene is nrdE [H]

Identifier: 119387082

GI number: 119387082

Start: 1567308

End: 1569434

Strand: Direct

Name: nrdE [H]

Synonym: Pden_4377

Alternate gene names: 119387082

Gene position: 1567308-1569434 (Clockwise)

Preceding gene: 119387081

Following gene: 119387083

Centisome position: 90.59

GC content: 63.0

Gene sequence:

>2127_bases
ATGCTTGACGATGGCGTAAAGGCCGAGCTGGACTATCACGCGCTGAATGCGATGCTGAACCTTTACGACGGCGAGGGCCG
CATCCGCTTCGACGCGGATCGCAAGGCCGCGCGGCAGTATTTCCTGCAGCATGTCAACCAGAACACCGTCTTCTTCCACT
CGCTGGACGAAAAGCTGGGCTATCTGGTCGACGAGGGCTATTACGAGGCCGAGGTGCTGGACCAGTATCCGCGCGCCTTC
CTGCACCGGATCTGGGACGCGGCCTATGCGCGGAAATTCCGCTTTCCGACCTTTCTCGGCGCCTTCAAGTATTACACCAG
CTATACGCTGAAGACCCGCGACGGGCGGCGCTATCTGGAACGCTACGAGGACCGGGTGGTCATGGTCGCGCTGGCGCTCG
CGCGCGGCGACGAGGATCTGGCCATGCGCTTCATGGAGGAAATCCTGTCGGGCCGGTTCCAGCCCGCCACGCCGACCTTC
CTGAACGCCGGCAAGAAGTCGCGGGGCGAGCTGATCTCGTGCTTCCTGCTGCGGCTGGAAGACAACATGGAATCCATCGG
CCGCGGCATCAATTCGGCGCTGCAGCTTTCCAAGCGCGGCGGCGGCGTCGCCCTGATGCTGACCAACCTGCGCGAGGCCG
GCGCGCCGATCAAGGGGATCGAGAACCAGTCCTCGGGCGTCATCCCGGTGATGAAACTGCTGGAGGACAGCTTTTCCTAT
GCCAACCAGCTTGGCGCGCGGCAAGGCGCGGGGGCGGTCTATCTGAACGCGCATCACCCCGACATCCTGCGCTTCCTGGA
CACCAAGCGCGAGAACGCCGACGAAAAGATCCGCATCAAGACGCTGTCGCTGGGCGTGGTGATCCCGGACGTGACCTTCG
AACTCGCCAAGCGCAACGAGGACATGTACCTGTTCTCGCCCCATGACGTGGAAAAGGTCTATGGCCTGCCCTTCTCGGAA
ATCTCGGTCACCGAGAAATATGCCGAGATGGTGGACGACAAGCGCATCCGCAAGAAAAAGATCAACGCCCGCGCCTTCTT
CCAGACGCTGGCCGAGATCCAGTTCGAAAGCGGCTATCCCTACATCATGTTCGAGGACACGGTGAACCGCGCCAACCCGG
TCGCCGGCCGCATCAGCATGTCGAACCTGTGCAGCGAGATCCTGCAGGTGAACGAGGCCAGTGAGTTCGACGAGGATCTG
GGCTATCGCCACCTCGGCACGGATATTTCCTGCAACCTCGGCTCGCTCAACATCGCCGCGACCATGGACGGGCCGGATTT
CGGCGCGACCGTCGAGGCGGCGATCCGGGCGCTGACCGCGGTGTCGGAGATGTCGGCCATCGAGGCCGTGCCCTCGATCC
GGCGCGGCAATGACGAGGCCCATGCCGTGGGGCTGGGCCAGATGAACCTGCACGGATTCCTGGCGCGCGAGCGCATCCAT
TACGGCAGCCCCGAGGGCGTCGAGTTCACCTCGGTCTATTTCGCCGCCGTGGCCTATCACGCCATCCGCGCCTCGAACCT
GCTGGCGCAGGAAAAGGGCCGGACCTTCAAGGATTTCGACAAGTCGAAATATGCCGACGGCTCGTTCTTCGACAAATACG
TCGCGCAGGACTGGCTGCCCACGCTGCCCGATGTCGCGCGGGTGTTCGAGGGCGTCAGCCTGCCCACCCGCGAGGATTGG
GCGGCACTGCGGGAATCGGTGATGCGGCACGGGCTTTACAACCGCAACCTGCAGGCGGTGCCGCCCACCGGCTCGATCAG
CTATATCAACAACTCGACCAGCTCGATCCATCCGATCGTGGCCAAGATCGAGATCCGCAAGGAAGGCAAGATCGGCCGGG
TCTATTACCCCGCCGCCTTCATGTCCAACGAGAACCTGGACTATTACCGCGACGCATACGAGATCGGACCCGAGGCGATC
ATCGACACCTATGCCGCCGCGACCGAGCATGTCGACCAGGGGTTGTCGCTGACGCTGTTCTTCCCGGCCGAGGCCACGAC
GCGCGACATCAACCGCGCCCAGATCTACGCCTGGAAGAAGGGCATCAAGACGATCTATTACATCCGGCTGCGCCAGACCG
CGCTGGAAGGCACCGAGGTGCAGGGCTGCGTTTCCTGCACGCTTTGA

Upstream 100 bases:

>100_bases
ACAAGTGCAACGTCCCCGTGCTGTATCGATTCGAGCTGGCGGGGACTGATCTGGACATTTCCCGCGTTCAGGCGGGGCTT
GCGAAATTCTGGGGGACGGA

Downstream 100 bases:

>100_bases
TCGTGGGGGCCTTGGGCCCCCACACCCCGAGGGTATTTGAGAAACGATGAAAGGGGTTCTTCGGATGAAGGATCATGCCA
TGCGGACGCCCGTGCGCGCG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MLDDGVKAELDYHALNAMLNLYDGEGRIRFDADRKAARQYFLQHVNQNTVFFHSLDEKLGYLVDEGYYEAEVLDQYPRAF
LHRIWDAAYARKFRFPTFLGAFKYYTSYTLKTRDGRRYLERYEDRVVMVALALARGDEDLAMRFMEEILSGRFQPATPTF
LNAGKKSRGELISCFLLRLEDNMESIGRGINSALQLSKRGGGVALMLTNLREAGAPIKGIENQSSGVIPVMKLLEDSFSY
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDVTFELAKRNEDMYLFSPHDVEKVYGLPFSE
ISVTEKYAEMVDDKRIRKKKINARAFFQTLAEIQFESGYPYIMFEDTVNRANPVAGRISMSNLCSEILQVNEASEFDEDL
GYRHLGTDISCNLGSLNIAATMDGPDFGATVEAAIRALTAVSEMSAIEAVPSIRRGNDEAHAVGLGQMNLHGFLARERIH
YGSPEGVEFTSVYFAAVAYHAIRASNLLAQEKGRTFKDFDKSKYADGSFFDKYVAQDWLPTLPDVARVFEGVSLPTREDW
AALRESVMRHGLYNRNLQAVPPTGSISYINNSTSSIHPIVAKIEIRKEGKIGRVYYPAAFMSNENLDYYRDAYEIGPEAI
IDTYAAATEHVDQGLSLTLFFPAEATTRDINRAQIYAWKKGIKTIYYIRLRQTALEGTEVQGCVSCTL

Sequences:

>Translated_708_residues
MLDDGVKAELDYHALNAMLNLYDGEGRIRFDADRKAARQYFLQHVNQNTVFFHSLDEKLGYLVDEGYYEAEVLDQYPRAF
LHRIWDAAYARKFRFPTFLGAFKYYTSYTLKTRDGRRYLERYEDRVVMVALALARGDEDLAMRFMEEILSGRFQPATPTF
LNAGKKSRGELISCFLLRLEDNMESIGRGINSALQLSKRGGGVALMLTNLREAGAPIKGIENQSSGVIPVMKLLEDSFSY
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDVTFELAKRNEDMYLFSPHDVEKVYGLPFSE
ISVTEKYAEMVDDKRIRKKKINARAFFQTLAEIQFESGYPYIMFEDTVNRANPVAGRISMSNLCSEILQVNEASEFDEDL
GYRHLGTDISCNLGSLNIAATMDGPDFGATVEAAIRALTAVSEMSAIEAVPSIRRGNDEAHAVGLGQMNLHGFLARERIH
YGSPEGVEFTSVYFAAVAYHAIRASNLLAQEKGRTFKDFDKSKYADGSFFDKYVAQDWLPTLPDVARVFEGVSLPTREDW
AALRESVMRHGLYNRNLQAVPPTGSISYINNSTSSIHPIVAKIEIRKEGKIGRVYYPAAFMSNENLDYYRDAYEIGPEAI
IDTYAAATEHVDQGLSLTLFFPAEATTRDINRAQIYAWKKGIKTIYYIRLRQTALEGTEVQGCVSCTL
>Mature_708_residues
MLDDGVKAELDYHALNAMLNLYDGEGRIRFDADRKAARQYFLQHVNQNTVFFHSLDEKLGYLVDEGYYEAEVLDQYPRAF
LHRIWDAAYARKFRFPTFLGAFKYYTSYTLKTRDGRRYLERYEDRVVMVALALARGDEDLAMRFMEEILSGRFQPATPTF
LNAGKKSRGELISCFLLRLEDNMESIGRGINSALQLSKRGGGVALMLTNLREAGAPIKGIENQSSGVIPVMKLLEDSFSY
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDVTFELAKRNEDMYLFSPHDVEKVYGLPFSE
ISVTEKYAEMVDDKRIRKKKINARAFFQTLAEIQFESGYPYIMFEDTVNRANPVAGRISMSNLCSEILQVNEASEFDEDL
GYRHLGTDISCNLGSLNIAATMDGPDFGATVEAAIRALTAVSEMSAIEAVPSIRRGNDEAHAVGLGQMNLHGFLARERIH
YGSPEGVEFTSVYFAAVAYHAIRASNLLAQEKGRTFKDFDKSKYADGSFFDKYVAQDWLPTLPDVARVFEGVSLPTREDW
AALRESVMRHGLYNRNLQAVPPTGSISYINNSTSSIHPIVAKIEIRKEGKIGRVYYPAAFMSNENLDYYRDAYEIGPEAI
IDTYAAATEHVDQGLSLTLFFPAEATTRDINRAQIYAWKKGIKTIYYIRLRQTALEGTEVQGCVSCTL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=652, Percent_Identity=26.0736196319018, Blast_Score=196, Evalue=7e-50,
Organism=Escherichia coli, GI1789030, Length=701, Percent_Identity=71.1840228245364, Blast_Score=1062, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=571, Percent_Identity=26.2697022767075, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17555242, Length=650, Percent_Identity=26.1538461538462, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322123, Length=656, Percent_Identity=25.9146341463415, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320914, Length=653, Percent_Identity=26.0336906584992, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI17352481, Length=646, Percent_Identity=26.7801857585139, Blast_Score=188, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 79766; Mature: 79766

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00089 RIBORED_LARGE ; PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDGVKAELDYHALNAMLNLYDGEGRIRFDADRKAARQYFLQHVNQNTVFFHSLDEKLG
CCCCCCCCHHHHHHHHHHHHEECCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEHHHHHC
YLVDEGYYEAEVLDQYPRAFLHRIWDAAYARKFRFPTFLGAFKYYTSYTLKTRDGRRYLE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCHHHHHH
RYEDRVVMVALALARGDEDLAMRFMEEILSGRFQPATPTFLNAGKKSRGELISCFLLRLE
HHHCHHEEEHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHH
DNMESIGRGINSALQLSKRGGGVALMLTNLREAGAPIKGIENQSSGVIPVMKLLEDSFSY
HCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDVTFELAKRNE
HHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHHHCCC
DMYLFSPHDVEKVYGLPFSEISVTEKYAEMVDDKRIRKKKINARAFFQTLAEIQFESGYP
CEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
YIMFEDTVNRANPVAGRISMSNLCSEILQVNEASEFDEDLGYRHLGTDISCNLGSLNIAA
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCEECCCCCEECCCCEEEEE
TMDGPDFGATVEAAIRALTAVSEMSAIEAVPSIRRGNDEAHAVGLGQMNLHGFLARERIH
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHC
YGSPEGVEFTSVYFAAVAYHAIRASNLLAQEKGRTFKDFDKSKYADGSFFDKYVAQDWLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCC
TLPDVARVFEGVSLPTREDWAALRESVMRHGLYNRNLQAVPPTGSISYINNSTSSIHPIV
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEECCCCCCCCEEE
AKIEIRKEGKIGRVYYPAAFMSNENLDYYRDAYEIGPEAIIDTYAAATEHVDQGLSLTLF
EEEEEECCCCCCEEEECHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEE
FPAEATTRDINRAQIYAWKKGIKTIYYIRLRQTALEGTEVQGCVSCTL
ECCCCCCCCCCHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCEEEECC
>Mature Secondary Structure
MLDDGVKAELDYHALNAMLNLYDGEGRIRFDADRKAARQYFLQHVNQNTVFFHSLDEKLG
CCCCCCCCHHHHHHHHHHHHEECCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEHHHHHC
YLVDEGYYEAEVLDQYPRAFLHRIWDAAYARKFRFPTFLGAFKYYTSYTLKTRDGRRYLE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCHHHHHH
RYEDRVVMVALALARGDEDLAMRFMEEILSGRFQPATPTFLNAGKKSRGELISCFLLRLE
HHHCHHEEEHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHH
DNMESIGRGINSALQLSKRGGGVALMLTNLREAGAPIKGIENQSSGVIPVMKLLEDSFSY
HCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDVTFELAKRNE
HHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHHHCCC
DMYLFSPHDVEKVYGLPFSEISVTEKYAEMVDDKRIRKKKINARAFFQTLAEIQFESGYP
CEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
YIMFEDTVNRANPVAGRISMSNLCSEILQVNEASEFDEDLGYRHLGTDISCNLGSLNIAA
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCEECCCCCEECCCCEEEEE
TMDGPDFGATVEAAIRALTAVSEMSAIEAVPSIRRGNDEAHAVGLGQMNLHGFLARERIH
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHC
YGSPEGVEFTSVYFAAVAYHAIRASNLLAQEKGRTFKDFDKSKYADGSFFDKYVAQDWLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCC
TLPDVARVFEGVSLPTREDWAALRESVMRHGLYNRNLQAVPPTGSISYINNSTSSIHPIV
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEECCCCCCCCEEE
AKIEIRKEGKIGRVYYPAAFMSNENLDYYRDAYEIGPEAIIDTYAAATEHVDQGLSLTLF
EEEEEECCCCCCEEEECHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEE
FPAEATTRDINRAQIYAWKKGIKTIYYIRLRQTALEGTEVQGCVSCTL
ECCCCCCCCCCHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]