Click here to switch to the map view.

The map label for this gene is gluQ [H]

Identifier: 119386786

GI number: 119386786

Start: 1234742

End: 1235587

Strand: Direct

Name: gluQ [H]

Synonym: Pden_4079

Alternate gene names: 119386786

Gene position: 1234742-1235587 (Clockwise)

Preceding gene: 119386785

Following gene: 119386788

Centisome position: 71.37

GC content: 66.9

Gene sequence:

>846_bases
TTGAACCGGACCCGGTTTGCCCCCTCGCCGACCGGCCCCCTGCATCTGGGCCATGCCTTCGCGGCCCTGACCGCGGCACG
CCTTGCCGATCCCGGCCAGTTCCTGCTGCGCATCGAGGATATCGACCAAAGCCGCTGCCGCCCGGAATACGAGGCGCTGA
TCGCCCAGGACCTGCGCTGGCTGGGCCTCGACTGGCCGCAGCCGGTCATGCGCCAGTCCGAACGCCTGCCCGCCTATCAA
GCGGCCCTCGACCGGCTCGCGGCGCTTGGCCTGACCTATCCCTGCCGCTGCCGCCGCGCCGATATTCGTGCCGCGCTTTC
CGCCCCGCAAGAAGGCGCGGCGCCAGTGATCGGCCCGGACGGCCCGGTCTATCCAGGCACCTGCCGCCATCGCCGCATGG
CGGATGCCGGGCCGGATGACGCCATCCGCCTGAATGCCGCGCGGGCGCTGAACCATCTCGGGCTCGACGAGGTCGCATTT
CATGACGAAAACGTAACCCCCGGCCTGCCGCATCGACTTTCGGCACGGGATTTCGTCGCTGGCGTCGGCGATATCGTGCT
GGCGCGGCGCGGCATGGGCACCAGCTATCACCTCTCGGTGGTGGTGGACGATGCCGACCAAGGCATCACCCTGGTCACAC
GTGGAAAAGATCTTCTGGATTCAACATGGATCCATATCATTCTTCAGAAACTGCTGGAACTTCCGGTGCCGCGCTATCAC
CATCACCGCCTGATCCGGGACGAGCATGGCAAGCGCCTTGCCAAGCGCGACGACGCCCGGGCGCTGAGGCTTTTCCGCGA
CGAAGGTGCCAGCGCCGCAGAAATCCGTCAGGCACTGGGGTTCTGA

Upstream 100 bases:

>100_bases
TGGACGCGATGCGCGGCGGCCGCAAGGGGCGCAAGGACCGCTACCCGGCCTATACGGACCGAGCGAAAGCCGATTTTCAG
CAATGGGTTGCGGGGGAAAG

Downstream 100 bases:

>100_bases
GGCGCCATGATGACGACCTCTTGCCCCTCGCGAATAGCGGTATAGAAACAGCTGCGCCGGTTGGTGTGGCAGGCCGGCCC
GGTCTGGTCCACCAGCAGCA

Product: glutamyl-tRNA synthetase, class Ic

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNRTRFAPSPTGPLHLGHAFAALTAARLADPGQFLLRIEDIDQSRCRPEYEALIAQDLRWLGLDWPQPVMRQSERLPAYQ
AALDRLAALGLTYPCRCRRADIRAALSAPQEGAAPVIGPDGPVYPGTCRHRRMADAGPDDAIRLNAARALNHLGLDEVAF
HDENVTPGLPHRLSARDFVAGVGDIVLARRGMGTSYHLSVVVDDADQGITLVTRGKDLLDSTWIHIILQKLLELPVPRYH
HHRLIRDEHGKRLAKRDDARALRLFRDEGASAAEIRQALGF

Sequences:

>Translated_281_residues
MNRTRFAPSPTGPLHLGHAFAALTAARLADPGQFLLRIEDIDQSRCRPEYEALIAQDLRWLGLDWPQPVMRQSERLPAYQ
AALDRLAALGLTYPCRCRRADIRAALSAPQEGAAPVIGPDGPVYPGTCRHRRMADAGPDDAIRLNAARALNHLGLDEVAF
HDENVTPGLPHRLSARDFVAGVGDIVLARRGMGTSYHLSVVVDDADQGITLVTRGKDLLDSTWIHIILQKLLELPVPRYH
HHRLIRDEHGKRLAKRDDARALRLFRDEGASAAEIRQALGF
>Mature_281_residues
MNRTRFAPSPTGPLHLGHAFAALTAARLADPGQFLLRIEDIDQSRCRPEYEALIAQDLRWLGLDWPQPVMRQSERLPAYQ
AALDRLAALGLTYPCRCRRADIRAALSAPQEGAAPVIGPDGPVYPGTCRHRRMADAGPDDAIRLNAARALNHLGLDEVAF
HDENVTPGLPHRLSARDFVAGVGDIVLARRGMGTSYHLSVVVDDADQGITLVTRGKDLLDSTWIHIILQKLLELPVPRYH
HHRLIRDEHGKRLAKRDDARALRLFRDEGASAAEIRQALGF

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=290, Percent_Identity=32.4137931034483, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788743, Length=271, Percent_Identity=34.3173431734317, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI226510925, Length=264, Percent_Identity=35.9848484848485, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=271, Percent_Identity=32.1033210332103, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324540, Length=277, Percent_Identity=30.3249097472924, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24665321, Length=282, Percent_Identity=31.5602836879433, Blast_Score=103, Evalue=9e-23,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 31129; Mature: 31129

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTRFAPSPTGPLHLGHAFAALTAARLADPGQFLLRIEDIDQSRCRPEYEALIAQDLRW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHH
LGLDWPQPVMRQSERLPAYQAALDRLAALGLTYPCRCRRADIRAALSAPQEGAAPVIGPD
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GPVYPGTCRHRRMADAGPDDAIRLNAARALNHLGLDEVAFHDENVTPGLPHRLSARDFVA
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHEECCCCCCCCCCCCCCHHHHHH
GVGDIVLARRGMGTSYHLSVVVDDADQGITLVTRGKDLLDSTWIHIILQKLLELPVPRYH
HHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHH
HHRLIRDEHGKRLAKRDDARALRLFRDEGASAAEIRQALGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MNRTRFAPSPTGPLHLGHAFAALTAARLADPGQFLLRIEDIDQSRCRPEYEALIAQDLRW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHH
LGLDWPQPVMRQSERLPAYQAALDRLAALGLTYPCRCRRADIRAALSAPQEGAAPVIGPD
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GPVYPGTCRHRRMADAGPDDAIRLNAARALNHLGLDEVAFHDENVTPGLPHRLSARDFVA
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHEECCCCCCCCCCCCCCHHHHHH
GVGDIVLARRGMGTSYHLSVVVDDADQGITLVTRGKDLLDSTWIHIILQKLLELPVPRYH
HHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHH
HHRLIRDEHGKRLAKRDDARALRLFRDEGASAAEIRQALGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA