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The map label for this gene is trmFO

Identifier: 119386785

GI number: 119386785

Start: 1233405

End: 1234745

Strand: Direct

Name: trmFO

Synonym: Pden_4078

Alternate gene names: 119386785

Gene position: 1233405-1234745 (Clockwise)

Preceding gene: 119386784

Following gene: 119386786

Centisome position: 71.29

GC content: 67.93

Gene sequence:

>1341_bases
ATGGAACCGATCCATATCATCGGCGCGGGTCTTGCCGGATCCGAGGCCGCCTGGCAGATCGCCCGCGCCGGCGTGCCGGT
GGTGCTGCACGAGATGCGGCCACAGGTCGCCACCTTCGCCCATCGCACCGGCGATTGCGCCGAGATGGTCTGCTCGAACA
GCTTCCGCTCGGACGACGACCGCATGAATGCCGTGGGGCAGCTGCATTGGGAGATGCGGGCGGCGGGCGGGCTGATCATG
GCCATGGCCGACCGCCATCGCCTGCCCGCCGGCGGCGCGCTGGCGGTCGACCGCGACGCCTTCTCGCAGGCCGTGACCCA
GGCGCTGCAAGCCGAACCGCTGGTCAGCTTCCAGCCAGGCGAGATCAGCGACCTGCCCTCCGAAGGGCACTGGATCGTCG
CCACCGGGCCGCTGACCTCCGAGGCGCTCGGGAGGTCGATCCGCAGCCTGACCGGCGAGGGCTCGCTCGCCTTCTTCGAC
GCCATCGCCCCCATCGTCCACGCCGAAAGCATCGACATGTCGATCTGCTGGCGCCAAAGCCGCTACGACAAGGGCGAGAC
CGAGGAAGAGCGCACCGCCTATATCAACTGCCCGATGACCCGCGAGGATTACGAGGGCTTCATCGACGCCCTGCTCGCCG
CCGACAAGACCGAGTTCCACGAGGGCGAGACCGCCGGCTATTTCGACGGCTGCCTGCCGATCGAGGTCATGGCCGAACGC
GGCCGCGAGACGCTGCGCCACGGGCCGATGAAACCCGTGGGCCTGACCAACGCCCATAACCCGGCCGAAAAGCCCTATGC
CGTGGTGCAGCTGCGTCGCGACAATGCGCTGGGGACGCTCTACAATATCGTCGGCTTCCAGACCAAGATGAAATATGGTG
CGCAAGTGGATGTTTTCCGGCGCATTCCCGGCCTGCAGGATGCCAGTTTCGCCCGGCTCGGCGGCATCCACCGCAATACG
TTCCTGAATTCGCCGCGGCTGATCGACGACCGGCTGCGGTTGCGCGCACGGCCGCATCTGCGCTTTGCCGGCCAGGTGAC
CGGGGTCGAAGGCTATGTCGAATCCGCGGCCATGGGCCTGCTCGCCGGGCGCATGGCCGCAGCCGAGGCGCTGGGGCGCG
ACCTGCCCCCACCGCCCGCCACCACCTCGATGGGCGCGCTGGTCAGCCACATCACCGGCGGCGCCGACGCGAAGACCTTC
CAGCCGATGAACGTGAATTTCGGCCTCTACCCGCCTCTGGACGCGATGCGCGGCGGCCGCAAGGGGCGCAAGGACCGCTA
CCCGGCCTATACGGACCGAGCGAAAGCCGATTTTCAGCAATGGGTTGCGGGGGAAAGTTGA

Upstream 100 bases:

>100_bases
TGAACGGAATCGGGCGGCACCCCTTGCGGGCGCCGCCCTTTCTCTTGCCGCCATGGCCCGCGATTGCTAGGAGATCGCGC
CTGACGCGAAGGGAAAAAGC

Downstream 100 bases:

>100_bases
ACCGGACCCGGTTTGCCCCCTCGCCGACCGGCCCCCTGCATCTGGGCCATGCCTTCGCGGCCCTGACCGCGGCACGCCTT
GCCGATCCCGGCCAGTTCCT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MEPIHIIGAGLAGSEAAWQIARAGVPVVLHEMRPQVATFAHRTGDCAEMVCSNSFRSDDDRMNAVGQLHWEMRAAGGLIM
AMADRHRLPAGGALAVDRDAFSQAVTQALQAEPLVSFQPGEISDLPSEGHWIVATGPLTSEALGRSIRSLTGEGSLAFFD
AIAPIVHAESIDMSICWRQSRYDKGETEEERTAYINCPMTREDYEGFIDALLAADKTEFHEGETAGYFDGCLPIEVMAER
GRETLRHGPMKPVGLTNAHNPAEKPYAVVQLRRDNALGTLYNIVGFQTKMKYGAQVDVFRRIPGLQDASFARLGGIHRNT
FLNSPRLIDDRLRLRARPHLRFAGQVTGVEGYVESAAMGLLAGRMAAAEALGRDLPPPPATTSMGALVSHITGGADAKTF
QPMNVNFGLYPPLDAMRGGRKGRKDRYPAYTDRAKADFQQWVAGES

Sequences:

>Translated_446_residues
MEPIHIIGAGLAGSEAAWQIARAGVPVVLHEMRPQVATFAHRTGDCAEMVCSNSFRSDDDRMNAVGQLHWEMRAAGGLIM
AMADRHRLPAGGALAVDRDAFSQAVTQALQAEPLVSFQPGEISDLPSEGHWIVATGPLTSEALGRSIRSLTGEGSLAFFD
AIAPIVHAESIDMSICWRQSRYDKGETEEERTAYINCPMTREDYEGFIDALLAADKTEFHEGETAGYFDGCLPIEVMAER
GRETLRHGPMKPVGLTNAHNPAEKPYAVVQLRRDNALGTLYNIVGFQTKMKYGAQVDVFRRIPGLQDASFARLGGIHRNT
FLNSPRLIDDRLRLRARPHLRFAGQVTGVEGYVESAAMGLLAGRMAAAEALGRDLPPPPATTSMGALVSHITGGADAKTF
QPMNVNFGLYPPLDAMRGGRKGRKDRYPAYTDRAKADFQQWVAGES
>Mature_446_residues
MEPIHIIGAGLAGSEAAWQIARAGVPVVLHEMRPQVATFAHRTGDCAEMVCSNSFRSDDDRMNAVGQLHWEMRAAGGLIM
AMADRHRLPAGGALAVDRDAFSQAVTQALQAEPLVSFQPGEISDLPSEGHWIVATGPLTSEALGRSIRSLTGEGSLAFFD
AIAPIVHAESIDMSICWRQSRYDKGETEEERTAYINCPMTREDYEGFIDALLAADKTEFHEGETAGYFDGCLPIEVMAER
GRETLRHGPMKPVGLTNAHNPAEKPYAVVQLRRDNALGTLYNIVGFQTKMKYGAQVDVFRRIPGLQDASFARLGGIHRNT
FLNSPRLIDDRLRLRARPHLRFAGQVTGVEGYVESAAMGLLAGRMAAAEALGRDLPPPPATTSMGALVSHITGGADAKTF
QPMNVNFGLYPPLDAMRGGRKGRKDRYPAYTDRAKADFQQWVAGES

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_PARDP (A1B9F0)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_917840.1
- ProteinModelPortal:   A1B9F0
- STRING:   A1B9F0
- GeneID:   4582629
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_4078
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 48563; Mature: 48563

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIHIIGAGLAGSEAAWQIARAGVPVVLHEMRPQVATFAHRTGDCAEMVCSNSFRSDDD
CCCEEEEECCCCCCHHHHHHHHCCCCEEHHHHCHHHHHHHHCCCHHHHHHHCCCCCCCHH
RMNAVGQLHWEMRAAGGLIMAMADRHRLPAGGALAVDRDAFSQAVTQALQAEPLVSFQPG
HHHHHHHHHHHHHHCCCEEEEEHHCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCEECCC
EISDLPSEGHWIVATGPLTSEALGRSIRSLTGEGSLAFFDAIAPIVHAESIDMSICWRQS
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHEEECHH
RYDKGETEEERTAYINCPMTREDYEGFIDALLAADKTEFHEGETAGYFDGCLPIEVMAER
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHH
GRETLRHGPMKPVGLTNAHNPAEKPYAVVQLRRDNALGTLYNIVGFQTKMKYGAQVDVFR
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHH
RIPGLQDASFARLGGIHRNTFLNSPRLIDDRLRLRARPHLRFAGQVTGVEGYVESAAMGL
HCCCCCCCCHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCEEEECEECCHHHHHHHHHHHH
LAGRMAAAEALGRDLPPPPATTSMGALVSHITGGADAKTFQPMNVNFGLYPPLDAMRGGR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHCCCC
KGRKDRYPAYTDRAKADFQQWVAGES
CCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEPIHIIGAGLAGSEAAWQIARAGVPVVLHEMRPQVATFAHRTGDCAEMVCSNSFRSDDD
CCCEEEEECCCCCCHHHHHHHHCCCCEEHHHHCHHHHHHHHCCCHHHHHHHCCCCCCCHH
RMNAVGQLHWEMRAAGGLIMAMADRHRLPAGGALAVDRDAFSQAVTQALQAEPLVSFQPG
HHHHHHHHHHHHHHCCCEEEEEHHCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCEECCC
EISDLPSEGHWIVATGPLTSEALGRSIRSLTGEGSLAFFDAIAPIVHAESIDMSICWRQS
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHEEECHH
RYDKGETEEERTAYINCPMTREDYEGFIDALLAADKTEFHEGETAGYFDGCLPIEVMAER
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHH
GRETLRHGPMKPVGLTNAHNPAEKPYAVVQLRRDNALGTLYNIVGFQTKMKYGAQVDVFR
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHH
RIPGLQDASFARLGGIHRNTFLNSPRLIDDRLRLRARPHLRFAGQVTGVEGYVESAAMGL
HCCCCCCCCHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCEEEECEECCHHHHHHHHHHHH
LAGRMAAAEALGRDLPPPPATTSMGALVSHITGGADAKTFQPMNVNFGLYPPLDAMRGGR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHCCCC
KGRKDRYPAYTDRAKADFQQWVAGES
CCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA