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The map label for this gene is maiA [H]

Identifier: 119386260

GI number: 119386260

Start: 697602

End: 698237

Strand: Direct

Name: maiA [H]

Synonym: Pden_3549

Alternate gene names: 119386260

Gene position: 697602-698237 (Clockwise)

Preceding gene: 119386259

Following gene: 119386261

Centisome position: 40.32

GC content: 70.13

Gene sequence:

>636_bases
ATGACGGTCGTGCTGCACGACTACTGGCGATCCTCCGCATCCTACCGGGTGCGGATCGCGCTGGCGCTGAAGGGTATTGC
CTACGACCGGGTGGCGGTGGATCTGGTGGCCGGTGCGCAGCGGCGCGCGGATCATCTGGCGCTGAACCCGCAGGGGCTGG
TGCCGGTGCTGGAGATCGACGGGCTGCGGCTGACGCAGTCGCTGGCGATCCTGGAATACCTCGAGGAAACCCGGCCGCAG
CCGGCGCTGCTGCCCGAAGGTGCGGCGGCGCGGGCGCATGCCCGGGCGTTGGCGCTGGCGGTGGCCTGCGAGATCCATCC
GCTCTCGAATCTGGGGGTGCTGGCGCGGGTGGAGGCATTGGCCGGACCCGAGGCGCGCGCCGCCTGGAACCGCGAGAACA
TCGCCCGCGGCCTTGAGGCCGTCGAGCGGCTGCTGGACCATCCGGGCTTTGCCGGGCGGTTCTGCCATAGCGACAGGCCG
GGCATGGCCGATTGCGTGCTGATCCCGCAGATCTACAACGCCAGCCGCTGGGGGGTGGAGTTCCGACACCTGCCGCGCAT
CGCCGCCGTTGCATCGTCCTGCTCGGATTTGGCGGCATTCCAGATGGCCGCGCCGGAAAGAGTCCAGGTCGCCTGA

Upstream 100 bases:

>100_bases
TCGAGGACGGCGACGAGATCGGGCTGTTCGCCCAGGCGCGGGGCGAGGGCTATCGCATCGGCTTCGGCCCCTGCACCGGG
CAGGTCCTGCCTGCGCTGCC

Downstream 100 bases:

>100_bases
ACCGGCCCGGCCGGTTCGCGACCGTGTCCTTTGGAGGCCGGGGCTTGCGGATGCTGCGGCCCTTTCCGCCGTCGCTGCCT
TGTGGAATCGGGTCCTGTTC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTVVLHDYWRSSASYRVRIALALKGIAYDRVAVDLVAGAQRRADHLALNPQGLVPVLEIDGLRLTQSLAILEYLEETRPQ
PALLPEGAAARAHARALALAVACEIHPLSNLGVLARVEALAGPEARAAWNRENIARGLEAVERLLDHPGFAGRFCHSDRP
GMADCVLIPQIYNASRWGVEFRHLPRIAAVASSCSDLAAFQMAAPERVQVA

Sequences:

>Translated_211_residues
MTVVLHDYWRSSASYRVRIALALKGIAYDRVAVDLVAGAQRRADHLALNPQGLVPVLEIDGLRLTQSLAILEYLEETRPQ
PALLPEGAAARAHARALALAVACEIHPLSNLGVLARVEALAGPEARAAWNRENIARGLEAVERLLDHPGFAGRFCHSDRP
GMADCVLIPQIYNASRWGVEFRHLPRIAAVASSCSDLAAFQMAAPERVQVA
>Mature_210_residues
TVVLHDYWRSSASYRVRIALALKGIAYDRVAVDLVAGAQRRADHLALNPQGLVPVLEIDGLRLTQSLAILEYLEETRPQP
ALLPEGAAARAHARALALAVACEIHPLSNLGVLARVEALAGPEARAAWNRENIARGLEAVERLLDHPGFAGRFCHSDRPG
MADCVLIPQIYNASRWGVEFRHLPRIAAVASSCSDLAAFQMAAPERVQVA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=206, Percent_Identity=44.1747572815534, Blast_Score=164, Evalue=4e-41,
Organism=Homo sapiens, GI22202622, Length=154, Percent_Identity=41.5584415584416, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI194440732, Length=206, Percent_Identity=33.9805825242718, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17551302, Length=207, Percent_Identity=39.6135265700483, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17510461, Length=205, Percent_Identity=35.609756097561, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17556142, Length=205, Percent_Identity=36.0975609756098, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI45553325, Length=204, Percent_Identity=42.156862745098, Blast_Score=153, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24645375, Length=204, Percent_Identity=42.156862745098, Blast_Score=153, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21355859, Length=204, Percent_Identity=42.156862745098, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21355857, Length=205, Percent_Identity=39.0243902439024, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 22893; Mature: 22761

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVLHDYWRSSASYRVRIALALKGIAYDRVAVDLVAGAQRRADHLALNPQGLVPVLEID
CEEEEECHHCCCCCEEEEEEEEECCCHHHHHHHHHHHCHHHHHHHEEECCCCCEEEEEEC
GLRLTQSLAILEYLEETRPQPALLPEGAAARAHARALALAVACEIHPLSNLGVLARVEAL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHH
AGPEARAAWNRENIARGLEAVERLLDHPGFAGRFCHSDRPGMADCVLIPQIYNASRWGVE
CCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHCCHHCCCH
FRHLPRIAAVASSCSDLAAFQMAAPERVQVA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure 
TVVLHDYWRSSASYRVRIALALKGIAYDRVAVDLVAGAQRRADHLALNPQGLVPVLEID
EEEEECHHCCCCCEEEEEEEEECCCHHHHHHHHHHHCHHHHHHHEEECCCCCEEEEEEC
GLRLTQSLAILEYLEETRPQPALLPEGAAARAHARALALAVACEIHPLSNLGVLARVEAL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHH
AGPEARAAWNRENIARGLEAVERLLDHPGFAGRFCHSDRPGMADCVLIPQIYNASRWGVE
CCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHCCHHCCCH
FRHLPRIAAVASSCSDLAAFQMAAPERVQVA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10220173; 11481430 [H]