Click here to switch to the map view.

The map label for this gene is hypF [H]

Identifier: 119385818

GI number: 119385818

Start: 280745

End: 282994

Strand: Direct

Name: hypF [H]

Synonym: Pden_3096

Alternate gene names: 119385818

Gene position: 280745-282994 (Clockwise)

Preceding gene: 119385817

Following gene: 119385819

Centisome position: 16.23

GC content: 68.84

Gene sequence:

>2250_bases
ATGACTTTGCCGACCATGGAACAGGGCTTCGCCATCCGGGTGCGCGGGCAGGTGCAGGGGGTCGGCTTCCGGCCCTTTGT
CTGGCGGCTGGCGCGGGCGGCAGGGTTGCGCGGCGAAGTTCTGAACGATGCCGAAGGGGTCCTGATCCGTCTGGTCGCGC
AGGATTGCGGCGACTTCATCGCGGCCTTGCGCGCGGATAGGCCGCCTCTGGCCAGAATCGACGCGATCGAGGCCGTGCCA
CAGGTTTTTGCCGCGATGCCCGAGGGGTTTTCCATCGTGGCCAGCACGGGCACAGGCGCCGAAACGCGGGTCACGCCCGA
TGCCGCGACCTGCCCGGAATGCCGGGCTGAGGTCTTTGCGCCCGGTAGGCGGCAGGGCTATGCCTTTACCAACTGCACCC
ATTGCGGGCCGCGCTATACGATCCTGCGCGAACTGCCCTATGACCGGGCGCGAACCACGATGGCGGGCTTTGATCTTTGC
CCCGATTGTGCCGCCGAATATGCCGATCCCGCCGACCGTCGCTTTCACGCGCAACCCATCGCCTGCCCGGCCTGCGGCCC
AAACCTGTGGTATGAGGCCGGGGGAAAAGAGGTCGCGGGCGATCCGGTCGCATTGGCGGTGGCGGCGCTGAAGGCGGGGC
AGGTCGTCGCGGTCAAGGGGCTGGGCGGCTTTCACCTTGCCTGCGATGCGGCCGATGCCAGGGCCGTGGCGCTGTTGCGG
GCGCGCAAAAGGCGGCCGTCGAAGCCCTTTGCCCTGATGGGGACCGAGGCGATGATCTGCGCCCATGCGACGCCGTCCGA
GGCTTGCTGGCGATTGTTGCGCGACCCGGCGGCGCCGGTGGTGCTGGTGCCCGCCCGGGGCACATTGCCCGAGGATGTGG
CGCCGGGGATGCGCGCGCTTGGCGTCATGCTGCCCTATACGCCTCTGCATCACCTGCTGATTGCCGCTTTCGGGGGGATG
CTGGTGATGACCTCGGGCAATCTTTCGGGCGCCCCGCAGGTCACCGGCAATGACGAGGCGCGCAAGGCGCTGGCGCGCTT
TGCCGATGGTTTCCTGATGCACAACCGCGAGATTGCGCGGCGGCTGGATGACGGGGTCGAGCGCGCCGATCCGCCAATGG
TGATCCGCCGCGCCCGTGGGCGGGTGCCGGGCACGCTGCCCCTGCCGCCGGGGTTTGACGGAGCGCCTCGGGTGCTGGCC
TTTGGTGGTCAGATGAAGGCGGCGATCTGCCTGACCAAGAACGGTCAGGCGATGGTGTCGCAGCATCTGGGCGATCTGGA
CGATGCCGAGACCGCGCTGGAATACGACCGCACCCTGCGCGATCTGGCGGCCCTGTTCGACCATGCCCCCGAACTGGCCG
CCTGTGACCCGCATCCGGGCTATCGCGCCACGATAGCGGCCGAAGGCGCGGGCCTGCCGCTGATCCGGGTCTGGCACCAT
CATGCGCATCTGGCCTCGTGTCTTGGCGATAATCTCTGGCCACTGGACGGCGGCAGGGTGGCGGGGATCGTGATGGACGG
GCTTGGCCTTGGACCGGAAGGCGCGATCTGGGGCGGCGAGGTGTTGCTGGGCGATTATACCGGGTTCGAGCGCGTCGCGC
ATCTGGCCCCCGCCCCCTTGCCGGGCGGCGACAGCGCCAGTCGAGAGCCCTGGCGCAATGCGCTGGCGCGTCTGGATCAG
GCGGGGCTTTCAGCCGTGGCGGATCGGCTTTTCCCGGCCGCGCCCCGCGCTCTTCTGCGGCAGGCGGCCCGTGCGGGGGT
GAATGCGCCACAATCATCCTCGGCCGGGCGGCTGTTCGATGCCTTTGCGGCGCTTGCGGGCTTTGCCGGGGCGCAGAGTT
ACGAGGGCGAGGCCGCGATGCGGCTGGAGGCCATGGCGTGCCCTGCTTCGCCCTATCCGTTTGACTGCCGGGATGGGGGG
GTGATTGACCCCGCGCCGATGTTTCGCGCCGCCGCCACCGATCTGGCGGCAGGGCGCGATCCGGGCGAAATGGCGGGGGC
CTTTCATGCCGGGCTGGCGCGTGCCTTCTGCGCGCCGGCCCGCGCGCTGGTCCAGAGCGGCAGAGCGCAGGCGGTGGCCC
TGTCGGGCGGTGTGCTTCAGAATGCTTTCTTTTTGCGCGAATGTCTGGCGGCGCTGGATGGTCTGCCGGTCTTGCTGCAT
CGCCAGATACCGGCCAATGACGGAGGGCTTGCTTTCGGTCAGGCTCTGGTTGCTGCGGCGCAGCATATGGCTGCGGCGAA
AAGCTTGTAA

Upstream 100 bases:

>100_bases
AGCCGGGCGAGACGACCCCGCTCAGCGTGCAGCATGTGGTGCGCAGCTTTGATCCGTGCATGGTGTGCACCGTGCATTGA
GGAAGGAGTGCCCGCCGGAC

Downstream 100 bases:

>100_bases
CCGTCAGGCGCGTCCTCTGGCTGGATGCTGGCCATCCGGCCAATGTTGAAAATATCTCATGGCATTGAAAATGTTTAAGA
AAATGTAATTCTCCATCTGC

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MTLPTMEQGFAIRVRGQVQGVGFRPFVWRLARAAGLRGEVLNDAEGVLIRLVAQDCGDFIAALRADRPPLARIDAIEAVP
QVFAAMPEGFSIVASTGTGAETRVTPDAATCPECRAEVFAPGRRQGYAFTNCTHCGPRYTILRELPYDRARTTMAGFDLC
PDCAAEYADPADRRFHAQPIACPACGPNLWYEAGGKEVAGDPVALAVAALKAGQVVAVKGLGGFHLACDAADARAVALLR
ARKRRPSKPFALMGTEAMICAHATPSEACWRLLRDPAAPVVLVPARGTLPEDVAPGMRALGVMLPYTPLHHLLIAAFGGM
LVMTSGNLSGAPQVTGNDEARKALARFADGFLMHNREIARRLDDGVERADPPMVIRRARGRVPGTLPLPPGFDGAPRVLA
FGGQMKAAICLTKNGQAMVSQHLGDLDDAETALEYDRTLRDLAALFDHAPELAACDPHPGYRATIAAEGAGLPLIRVWHH
HAHLASCLGDNLWPLDGGRVAGIVMDGLGLGPEGAIWGGEVLLGDYTGFERVAHLAPAPLPGGDSASREPWRNALARLDQ
AGLSAVADRLFPAAPRALLRQAARAGVNAPQSSSAGRLFDAFAALAGFAGAQSYEGEAAMRLEAMACPASPYPFDCRDGG
VIDPAPMFRAAATDLAAGRDPGEMAGAFHAGLARAFCAPARALVQSGRAQAVALSGGVLQNAFFLRECLAALDGLPVLLH
RQIPANDGGLAFGQALVAAAQHMAAAKSL

Sequences:

>Translated_749_residues
MTLPTMEQGFAIRVRGQVQGVGFRPFVWRLARAAGLRGEVLNDAEGVLIRLVAQDCGDFIAALRADRPPLARIDAIEAVP
QVFAAMPEGFSIVASTGTGAETRVTPDAATCPECRAEVFAPGRRQGYAFTNCTHCGPRYTILRELPYDRARTTMAGFDLC
PDCAAEYADPADRRFHAQPIACPACGPNLWYEAGGKEVAGDPVALAVAALKAGQVVAVKGLGGFHLACDAADARAVALLR
ARKRRPSKPFALMGTEAMICAHATPSEACWRLLRDPAAPVVLVPARGTLPEDVAPGMRALGVMLPYTPLHHLLIAAFGGM
LVMTSGNLSGAPQVTGNDEARKALARFADGFLMHNREIARRLDDGVERADPPMVIRRARGRVPGTLPLPPGFDGAPRVLA
FGGQMKAAICLTKNGQAMVSQHLGDLDDAETALEYDRTLRDLAALFDHAPELAACDPHPGYRATIAAEGAGLPLIRVWHH
HAHLASCLGDNLWPLDGGRVAGIVMDGLGLGPEGAIWGGEVLLGDYTGFERVAHLAPAPLPGGDSASREPWRNALARLDQ
AGLSAVADRLFPAAPRALLRQAARAGVNAPQSSSAGRLFDAFAALAGFAGAQSYEGEAAMRLEAMACPASPYPFDCRDGG
VIDPAPMFRAAATDLAAGRDPGEMAGAFHAGLARAFCAPARALVQSGRAQAVALSGGVLQNAFFLRECLAALDGLPVLLH
RQIPANDGGLAFGQALVAAAQHMAAAKSL
>Mature_748_residues
TLPTMEQGFAIRVRGQVQGVGFRPFVWRLARAAGLRGEVLNDAEGVLIRLVAQDCGDFIAALRADRPPLARIDAIEAVPQ
VFAAMPEGFSIVASTGTGAETRVTPDAATCPECRAEVFAPGRRQGYAFTNCTHCGPRYTILRELPYDRARTTMAGFDLCP
DCAAEYADPADRRFHAQPIACPACGPNLWYEAGGKEVAGDPVALAVAALKAGQVVAVKGLGGFHLACDAADARAVALLRA
RKRRPSKPFALMGTEAMICAHATPSEACWRLLRDPAAPVVLVPARGTLPEDVAPGMRALGVMLPYTPLHHLLIAAFGGML
VMTSGNLSGAPQVTGNDEARKALARFADGFLMHNREIARRLDDGVERADPPMVIRRARGRVPGTLPLPPGFDGAPRVLAF
GGQMKAAICLTKNGQAMVSQHLGDLDDAETALEYDRTLRDLAALFDHAPELAACDPHPGYRATIAAEGAGLPLIRVWHHH
AHLASCLGDNLWPLDGGRVAGIVMDGLGLGPEGAIWGGEVLLGDYTGFERVAHLAPAPLPGGDSASREPWRNALARLDQA
GLSAVADRLFPAAPRALLRQAARAGVNAPQSSSAGRLFDAFAALAGFAGAQSYEGEAAMRLEAMACPASPYPFDCRDGGV
IDPAPMFRAAATDLAAGRDPGEMAGAFHAGLARAFCAPARALVQSGRAQAVALSGGVLQNAFFLRECLAALDGLPVLLHR
QIPANDGGLAFGQALVAAAQHMAAAKSL

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=752, Percent_Identity=44.5478723404255, Blast_Score=551, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 78545; Mature: 78414

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPTMEQGFAIRVRGQVQGVGFRPFVWRLARAAGLRGEVLNDAEGVLIRLVAQDCGDFI
CCCCCCCCCEEEEEECEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
AALRADRPPLARIDAIEAVPQVFAAMPEGFSIVASTGTGAETRVTPDAATCPECRAEVFA
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCHHHHHHHCC
PGRRQGYAFTNCTHCGPRYTILRELPYDRARTTMAGFDLCPDCAAEYADPADRRFHAQPI
CCCCCCCEECCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCHHCCCCCCCC
ACPACGPNLWYEAGGKEVAGDPVALAVAALKAGQVVAVKGLGGFHLACDAADARAVALLR
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHH
ARKRRPSKPFALMGTEAMICAHATPSEACWRLLRDPAAPVVLVPARGTLPEDVAPGMRAL
HHHCCCCCCEEEECCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHCCHHHHH
GVMLPYTPLHHLLIAAFGGMLVMTSGNLSGAPQVTGNDEARKALARFADGFLMHNREIAR
HHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLDDGVERADPPMVIRRARGRVPGTLPLPPGFDGAPRVLAFGGQMKAAICLTKNGQAMVS
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCCHHHHH
QHLGDLDDAETALEYDRTLRDLAALFDHAPELAACDPHPGYRATIAAEGAGLPLIRVWHH
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
HAHLASCLGDNLWPLDGGRVAGIVMDGLGLGPEGAIWGGEVLLGDYTGFERVAHLAPAPL
HHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCEEEECCCCHHHHHHHCCCCC
PGGDSASREPWRNALARLDQAGLSAVADRLFPAAPRALLRQAARAGVNAPQSSSAGRLFD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
AFAALAGFAGAQSYEGEAAMRLEAMACPASPYPFDCRDGGVIDPAPMFRAAATDLAAGRD
HHHHHHHHCCCCCCCCCHHHEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PGEMAGAFHAGLARAFCAPARALVQSGRAQAVALSGGVLQNAFFLRECLAALDGLPVLLH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEE
RQIPANDGGLAFGQALVAAAQHMAAAKSL
ECCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLPTMEQGFAIRVRGQVQGVGFRPFVWRLARAAGLRGEVLNDAEGVLIRLVAQDCGDFI
CCCCCCCCEEEEEECEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
AALRADRPPLARIDAIEAVPQVFAAMPEGFSIVASTGTGAETRVTPDAATCPECRAEVFA
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCHHHHHHHCC
PGRRQGYAFTNCTHCGPRYTILRELPYDRARTTMAGFDLCPDCAAEYADPADRRFHAQPI
CCCCCCCEECCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCHHCCCCCCCC
ACPACGPNLWYEAGGKEVAGDPVALAVAALKAGQVVAVKGLGGFHLACDAADARAVALLR
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHH
ARKRRPSKPFALMGTEAMICAHATPSEACWRLLRDPAAPVVLVPARGTLPEDVAPGMRAL
HHHCCCCCCEEEECCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHCCHHHHH
GVMLPYTPLHHLLIAAFGGMLVMTSGNLSGAPQVTGNDEARKALARFADGFLMHNREIAR
HHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLDDGVERADPPMVIRRARGRVPGTLPLPPGFDGAPRVLAFGGQMKAAICLTKNGQAMVS
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCCHHHHH
QHLGDLDDAETALEYDRTLRDLAALFDHAPELAACDPHPGYRATIAAEGAGLPLIRVWHH
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
HAHLASCLGDNLWPLDGGRVAGIVMDGLGLGPEGAIWGGEVLLGDYTGFERVAHLAPAPL
HHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCEEEECCCCHHHHHHHCCCCC
PGGDSASREPWRNALARLDQAGLSAVADRLFPAAPRALLRQAARAGVNAPQSSSAGRLFD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
AFAALAGFAGAQSYEGEAAMRLEAMACPASPYPFDCRDGGVIDPAPMFRAAATDLAAGRD
HHHHHHHHCCCCCCCCCHHHEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PGEMAGAFHAGLARAFCAPARALVQSGRAQAVALSGGVLQNAFFLRECLAALDGLPVLLH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEE
RQIPANDGGLAFGQALVAAAQHMAAAKSL
ECCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497190; 9492269 [H]