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The map label for this gene is hybC [C]

Identifier: 119385817

GI number: 119385817

Start: 279291

End: 280724

Strand: Direct

Name: hybC [C]

Synonym: Pden_3095

Alternate gene names: 119385817

Gene position: 279291-280724 (Clockwise)

Preceding gene: 119385816

Following gene: 119385818

Centisome position: 16.14

GC content: 68.13

Gene sequence:

>1434_bases
ATGGGGCAGGGGCAGTTGATCGTCGGTCCGTTCAACCGGGTCGAGGGCGATCTGGAGATCCGGCTGGACATCGCAGAGGG
GCGGGTCAGCGCGGCGCGGATCAACTCGCCGCTTTATCGCGGGTTCGAGCGTATGCTGCCTGGCAAGGATCCGCGCGACG
CGCTGACCATCGTGCCGCGGATTTGCGGTATCTGCTCGATCAGCCAGTCGGCGGCGGCGGCGCGGGCTCTGGCCATGGCA
GTGGGCGAGGTCCCCGAACCGCAAGGAGAGCGGATGGCTGCGCTGATCCACGCGGTCGAGAATGTCTCGGACCATCTGAC
GCATTTCAACCTGTTCTTCATGCCTGATTTCACCAGACCCCAATATCAGGCGCGGGGCTGGTATGATCGGGCGGTGGCCC
GGTTCACGGCGATGGAGGGATCGGCCCTGAAACGCGCGGTCGAGGCGCGGGCGGGGTTGATGCATGTCCTTGGGCTGATG
GCCGGGAAATGGCCGCATACCCTGGCGATCCAGCCCGGTGGTGTCACGCGCGCCCCGTCCGAACGCGACCGGCTGCGTAT
TCTGGCCAGCCTGACGGCCTTTCGCCGCTATCTGGAAGATACGGTCTTCGGGGCAGCGGTCGAGGATTTCGCGGCCCTTT
CCTCGGTCCCGGCGCTGATGGGATGGCAAGGGGGCGATGCCGGCCTGTTCCTGCGCATCGCCGCCGATCTGAATCTGGCG
GATCTGGGGTGCGGCTCGGGGCGCTATCTGTCCTTTGGCGCCTATCCGCTGGAGGGTGGCCACGGCTTTGCCCCCGGCAT
CTGGGATGGCGGGGCGCAGCCTCTCGACATCGCGGCGATCAGCGAGGATTTGTCGCATAGCTGGATGCTGGGGCCTGCGG
CTCATCCGGCCCAGGGCGTGACCGATCCCGACGAGGATATGCGCGACACCGCCTATAGCTGGTGCAAGGCCCCCAGACTG
GCCGGGCGCACCATGGAAACCGGCGCGCTGGCCCGGCAGGTGATCGACGGCCATCCGCTGGCGCTGGATCTGGTCCGCGA
TGGCGGCGGCGGGGTGCTGGCGCGGGTGGCGGGGCGTTTGCTGGAACTGGCGCGCAGTCAGGTTCTGATGGAGGATCTGG
CGCGCGGGATCGTGCCCGAGGCCCGCTTCATGGCCGGCCCCCTGAAAATCAGCGACGGGATCGGCGAAGGGCTGGTCGAG
GCCGCGCGCGGCGCGCTTGGCCATTGGCTGAGGATCGAAGGCGGGCGGATCGCCTCGTATCAGATCGTCGCTCCGACCAC
CTGGAACTTCAGCCCGCGCGACGCCGGCGGCGTGCCCGGCCCGGTCGAGGCGGCGCTTGAGGGGCTTGTGGTGCAGCCGG
GCGAGACGACCCCGCTCAGCGTGCAGCATGTGGTGCGCAGCTTTGATCCGTGCATGGTGTGCACCGTGCATTGA

Upstream 100 bases:

>100_bases
AGGCCGCCACGCCCGAGCGGATCGCGAAGAATGCCGTGGCCGATCGTATCGTCACCCCGCCGACGCTGAGAAAACCCCGA
TGAGCGGGGCAGGGGGCGAG

Downstream 100 bases:

>100_bases
GGAAGGAGTGCCCGCCGGACATGACTTTGCCGACCATGGAACAGGGCTTCGCCATCCGGGTGCGCGGGCAGGTGCAGGGG
GTCGGCTTCCGGCCCTTTGT

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MGQGQLIVGPFNRVEGDLEIRLDIAEGRVSAARINSPLYRGFERMLPGKDPRDALTIVPRICGICSISQSAAAARALAMA
VGEVPEPQGERMAALIHAVENVSDHLTHFNLFFMPDFTRPQYQARGWYDRAVARFTAMEGSALKRAVEARAGLMHVLGLM
AGKWPHTLAIQPGGVTRAPSERDRLRILASLTAFRRYLEDTVFGAAVEDFAALSSVPALMGWQGGDAGLFLRIAADLNLA
DLGCGSGRYLSFGAYPLEGGHGFAPGIWDGGAQPLDIAAISEDLSHSWMLGPAAHPAQGVTDPDEDMRDTAYSWCKAPRL
AGRTMETGALARQVIDGHPLALDLVRDGGGGVLARVAGRLLELARSQVLMEDLARGIVPEARFMAGPLKISDGIGEGLVE
AARGALGHWLRIEGGRIASYQIVAPTTWNFSPRDAGGVPGPVEAALEGLVVQPGETTPLSVQHVVRSFDPCMVCTVH

Sequences:

>Translated_477_residues
MGQGQLIVGPFNRVEGDLEIRLDIAEGRVSAARINSPLYRGFERMLPGKDPRDALTIVPRICGICSISQSAAAARALAMA
VGEVPEPQGERMAALIHAVENVSDHLTHFNLFFMPDFTRPQYQARGWYDRAVARFTAMEGSALKRAVEARAGLMHVLGLM
AGKWPHTLAIQPGGVTRAPSERDRLRILASLTAFRRYLEDTVFGAAVEDFAALSSVPALMGWQGGDAGLFLRIAADLNLA
DLGCGSGRYLSFGAYPLEGGHGFAPGIWDGGAQPLDIAAISEDLSHSWMLGPAAHPAQGVTDPDEDMRDTAYSWCKAPRL
AGRTMETGALARQVIDGHPLALDLVRDGGGGVLARVAGRLLELARSQVLMEDLARGIVPEARFMAGPLKISDGIGEGLVE
AARGALGHWLRIEGGRIASYQIVAPTTWNFSPRDAGGVPGPVEAALEGLVVQPGETTPLSVQHVVRSFDPCMVCTVH
>Mature_476_residues
GQGQLIVGPFNRVEGDLEIRLDIAEGRVSAARINSPLYRGFERMLPGKDPRDALTIVPRICGICSISQSAAAARALAMAV
GEVPEPQGERMAALIHAVENVSDHLTHFNLFFMPDFTRPQYQARGWYDRAVARFTAMEGSALKRAVEARAGLMHVLGLMA
GKWPHTLAIQPGGVTRAPSERDRLRILASLTAFRRYLEDTVFGAAVEDFAALSSVPALMGWQGGDAGLFLRIAADLNLAD
LGCGSGRYLSFGAYPLEGGHGFAPGIWDGGAQPLDIAAISEDLSHSWMLGPAAHPAQGVTDPDEDMRDTAYSWCKAPRLA
GRTMETGALARQVIDGHPLALDLVRDGGGGVLARVAGRLLELARSQVLMEDLARGIVPEARFMAGPLKISDGIGEGLVEA
ARGALGHWLRIEGGRIASYQIVAPTTWNFSPRDAGGVPGPVEAALEGLVVQPGETTPLSVQHVVRSFDPCMVCTVH

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=576, Percent_Identity=25.8680555555556, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1787207, Length=282, Percent_Identity=33.6879432624113, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 50889; Mature: 50758

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQGQLIVGPFNRVEGDLEIRLDIAEGRVSAARINSPLYRGFERMLPGKDPRDALTIVPR
CCCCCEEECCHHHCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHH
ICGICSISQSAAAARALAMAVGEVPEPQGERMAALIHAVENVSDHLTHFNLFFMPDFTRP
HHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCC
QYQARGWYDRAVARFTAMEGSALKRAVEARAGLMHVLGLMAGKWPHTLAIQPGGVTRAPS
CHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
ERDRLRILASLTAFRRYLEDTVFGAAVEDFAALSSVPALMGWQGGDAGLFLRIAADLNLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCCHH
DLGCGSGRYLSFGAYPLEGGHGFAPGIWDGGAQPLDIAAISEDLSHSWMLGPAAHPAQGV
CCCCCCCCEEEECCEECCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCEEECCCCCCCCCC
TDPDEDMRDTAYSWCKAPRLAGRTMETGALARQVIDGHPLALDLVRDGGGGVLARVAGRL
CCCCHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
LELARSQVLMEDLARGIVPEARFMAGPLKISDGIGEGLVEAARGALGHWLRIEGGRIASY
HHHHHHHHHHHHHHHCCCCCHHHHCCCEEECCCCHHHHHHHHHHHHCCEEEECCCEEEEE
QIVAPTTWNFSPRDAGGVPGPVEAALEGLVVQPGETTPLSVQHVVRSFDPCMVCTVH
EEECCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
GQGQLIVGPFNRVEGDLEIRLDIAEGRVSAARINSPLYRGFERMLPGKDPRDALTIVPR
CCCCEEECCHHHCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHH
ICGICSISQSAAAARALAMAVGEVPEPQGERMAALIHAVENVSDHLTHFNLFFMPDFTRP
HHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCC
QYQARGWYDRAVARFTAMEGSALKRAVEARAGLMHVLGLMAGKWPHTLAIQPGGVTRAPS
CHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
ERDRLRILASLTAFRRYLEDTVFGAAVEDFAALSSVPALMGWQGGDAGLFLRIAADLNLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCCHH
DLGCGSGRYLSFGAYPLEGGHGFAPGIWDGGAQPLDIAAISEDLSHSWMLGPAAHPAQGV
CCCCCCCCEEEECCEECCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCEEECCCCCCCCCC
TDPDEDMRDTAYSWCKAPRLAGRTMETGALARQVIDGHPLALDLVRDGGGGVLARVAGRL
CCCCHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
LELARSQVLMEDLARGIVPEARFMAGPLKISDGIGEGLVEAARGALGHWLRIEGGRIASY
HHHHHHHHHHHHHHHCCCCCHHHHCCCEEECCCCHHHHHHHHHHHHCCEEEECCCEEEEE
QIVAPTTWNFSPRDAGGVPGPVEAALEGLVVQPGETTPLSVQHVVRSFDPCMVCTVH
EEECCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]