Click here to switch to the map view.

The map label for this gene is fadJ [H]

Identifier: 119385596

GI number: 119385596

Start: 37026

End: 39209

Strand: Direct

Name: fadJ [H]

Synonym: Pden_2871

Alternate gene names: 119385596

Gene position: 37026-39209 (Clockwise)

Preceding gene: 119385595

Following gene: 119385599

Centisome position: 2.14

GC content: 66.9

Gene sequence:

>2184_bases
ATGACCGATTTCACCATGCAGAAAGACGCCGACGGCGTCGCGATCGTCACCTGGGACGTGCCGGGCAAGTCGATGAACGT
GCTGTCGCTGGACGGCGCCGCCGAGTTGAACGCACTGATCGACGACGCCCTGGCCGACGAGGCCGTGAAGGGCATCGTCA
TCACCAGCGGCAAGAAGGATTTCGCCGCCGGCATGGACCTGAACGTCATCGCCGGCATGAGGCAGGGCGGCGCGCAGGCG
GTCTTCGACGGCGTGATGACGCTGCATCATCTGCTGCGCAAGATCGAGCTGGCCGGGATGGACCCCAAGACCAAGAAGGG
CGGCAAGCCCATCGCGGCCGCCCTGCCCGGCACCGCACTGGGAATCGGGCTGGAACTGCCGCTGGCCACGCATCGCATCT
TTGCCGCCGAGAACCCCAAGGCCAAGATCGGCCTGCCCGAGATCATGGTCGGCATCTTTCCCGGCGCCGGCGGCACCACC
CGCCTGGTGCGCAAGCTGGGCGCCATGGGCGCCGCCCCCTTCCTGCTGGAGGGCAAGCTCAGCGACCCGGCCAGGGCCAA
GAGCGCCGGCCTGATCGACGAGGTGGTGGCCGATCCGGTCGCCGCGGCCCGCGAATGGGTGTTGAAGGCCGCCGATGCCG
ACCTGGTCAAGCCTTGGGACGCCAAGGGCTACAAGATGCCGGGCGGCGAGCCCTATCACCCGGCCGGCTTCATGACCTTC
GTCGGCGCCAGCGCCATGGTGCATGGCAAGACGCTGGGCGTCTATCCGGCGGCGAAGGCGCTGCTGAGCGCCGTCTATGA
AGGTGCCATGGTGCCCTTCGACACGGCGCTGAAGATCGAAGCGCGCTGGTTCACCCATGTGCTGATGAACCCCTCCTCGA
CCGCGATGATCCGCAGCCTCTTCATCAACAAGGAGGCGCTGGAGAAGGGCGCGAACCGTCCCGAGGCTCCCGATCAGAGC
GTGAGGAAGGTCGGCATCCTCGGCGCCGGCATGATGGGCGCCGGCATCGCGTATGTCTCGGCCATGGCCGGGATCAAGGT
GGTGCTGATCGATTCGACGCAAGAAGCGGCCGAGCGCGGCAAGTCCTACTCCACCGGCCTGCTGGACAAGGCGATCAGTC
GCCGCAAATCCACCGAGGAGAAGAAGGCCGAGGTGCTTTCCCGCATCACCGCGACGACCGATTACGCCGCACTGGCCGGC
TGCGACCTGATCGTGGAAGCCGTGTTCGAGGATCCGAAGGTCAAGGCCGAGGTCACGAAACGGGCCGAGGCGGTGATCCC
GCAGGACGCGATCTTCGCCACCAACACCTCGACCCTGCCGATCAGCGAACTGGCCAAGGCCAGCGCCCGCCCCGACCAGT
TCATCGGCATCCACTTCTTCAGCCCCGTGGACAAGATGCTGTTGGTCGAAATCATCAAGGGCCGCGAGACCGGCCCGCGC
GCGGTGGCCAAGGCGCTCGACTTCGTGCGCCAGATCCGCAAGACGCCCATCGTGGTCAACGACGCACGCTTCTTCTACGC
CAACCGCTGCATCATCCCCTATATCAACGAAGGCATCCGCATGGTGGCCGAAGGGGTCAGCCCCACGCTGATCGAAAACG
CCGCCAAGATGATGGGCATGCCGCTGGGACCGCTGCAACTGGTGGACGAGACCTCGATCGACCTCGGCGTCAAGATCGCC
AAGGCCACCAAGGCGGCGATGGGCGACGCCTACCCGGACGGCGCAGTGGATGCGGTGATCTTCAAGCTTGCCGACCAGGG
CCGGCTCGGCCGCAAGACCAAGGCCGGCTTCTACGACTATGACGATGCCGGCAAGCGCCAGGGCTTCTGGTCCGGCCTCG
CATCCGAATGGCCCGAGGCCGACACCCAGCCCGACCTGACCGAGATCCAGCACCGGCTGATGTTCGCGCAATCGCTGGAA
GCGGTCCGGGCGCTGGAAGAAGGCGTGCTCGAGGACATCCGCGAAGGCGATGTCGGCGCCATCCTCGGCTGGGGCTTCGC
GCCCTGGTCCGGCGGCCCCTTCGGCTGGCTCGACATGCTGGGCGCCCCCCGCGCCGCCGGAATCGCCGACAGCCTGGCGG
AAAAATTCGGCCCCCGCTTCGCCGCGCCGCAACTCCTCAGGGACATGGCTGCGAAAGGCGAAAGCTTCTACGGCCGCTTC
GGCGCCGGCAAACAGGCCGCCTGA

Upstream 100 bases:

>100_bases
AGCTGGAGCGCCAGGACAAGACCGTGGGCCTCGCCACGCTCTGCATCGCGTCCGGCATGGGCGCGGCCACCATCATCGAG
CGCGTCTGAGGGAGGGGAAC

Downstream 100 bases:

>100_bases
AAACGACCAGGGGGCAAGGCCACTCCGCCCTGCCCCTCACCGTTTCCCAAATACTCAAGAAAGACGAAGCGCCGAGGGCC
CGTCCCTTCGGCGCTTCTCT

Product: 3-hydroxyacyl-CoA dehydrogenase, NAD-binding

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MTDFTMQKDADGVAIVTWDVPGKSMNVLSLDGAAELNALIDDALADEAVKGIVITSGKKDFAAGMDLNVIAGMRQGGAQA
VFDGVMTLHHLLRKIELAGMDPKTKKGGKPIAAALPGTALGIGLELPLATHRIFAAENPKAKIGLPEIMVGIFPGAGGTT
RLVRKLGAMGAAPFLLEGKLSDPARAKSAGLIDEVVADPVAAAREWVLKAADADLVKPWDAKGYKMPGGEPYHPAGFMTF
VGASAMVHGKTLGVYPAAKALLSAVYEGAMVPFDTALKIEARWFTHVLMNPSSTAMIRSLFINKEALEKGANRPEAPDQS
VRKVGILGAGMMGAGIAYVSAMAGIKVVLIDSTQEAAERGKSYSTGLLDKAISRRKSTEEKKAEVLSRITATTDYAALAG
CDLIVEAVFEDPKVKAEVTKRAEAVIPQDAIFATNTSTLPISELAKASARPDQFIGIHFFSPVDKMLLVEIIKGRETGPR
AVAKALDFVRQIRKTPIVVNDARFFYANRCIIPYINEGIRMVAEGVSPTLIENAAKMMGMPLGPLQLVDETSIDLGVKIA
KATKAAMGDAYPDGAVDAVIFKLADQGRLGRKTKAGFYDYDDAGKRQGFWSGLASEWPEADTQPDLTEIQHRLMFAQSLE
AVRALEEGVLEDIREGDVGAILGWGFAPWSGGPFGWLDMLGAPRAAGIADSLAEKFGPRFAAPQLLRDMAAKGESFYGRF
GAGKQAA

Sequences:

>Translated_727_residues
MTDFTMQKDADGVAIVTWDVPGKSMNVLSLDGAAELNALIDDALADEAVKGIVITSGKKDFAAGMDLNVIAGMRQGGAQA
VFDGVMTLHHLLRKIELAGMDPKTKKGGKPIAAALPGTALGIGLELPLATHRIFAAENPKAKIGLPEIMVGIFPGAGGTT
RLVRKLGAMGAAPFLLEGKLSDPARAKSAGLIDEVVADPVAAAREWVLKAADADLVKPWDAKGYKMPGGEPYHPAGFMTF
VGASAMVHGKTLGVYPAAKALLSAVYEGAMVPFDTALKIEARWFTHVLMNPSSTAMIRSLFINKEALEKGANRPEAPDQS
VRKVGILGAGMMGAGIAYVSAMAGIKVVLIDSTQEAAERGKSYSTGLLDKAISRRKSTEEKKAEVLSRITATTDYAALAG
CDLIVEAVFEDPKVKAEVTKRAEAVIPQDAIFATNTSTLPISELAKASARPDQFIGIHFFSPVDKMLLVEIIKGRETGPR
AVAKALDFVRQIRKTPIVVNDARFFYANRCIIPYINEGIRMVAEGVSPTLIENAAKMMGMPLGPLQLVDETSIDLGVKIA
KATKAAMGDAYPDGAVDAVIFKLADQGRLGRKTKAGFYDYDDAGKRQGFWSGLASEWPEADTQPDLTEIQHRLMFAQSLE
AVRALEEGVLEDIREGDVGAILGWGFAPWSGGPFGWLDMLGAPRAAGIADSLAEKFGPRFAAPQLLRDMAAKGESFYGRF
GAGKQAA
>Mature_726_residues
TDFTMQKDADGVAIVTWDVPGKSMNVLSLDGAAELNALIDDALADEAVKGIVITSGKKDFAAGMDLNVIAGMRQGGAQAV
FDGVMTLHHLLRKIELAGMDPKTKKGGKPIAAALPGTALGIGLELPLATHRIFAAENPKAKIGLPEIMVGIFPGAGGTTR
LVRKLGAMGAAPFLLEGKLSDPARAKSAGLIDEVVADPVAAAREWVLKAADADLVKPWDAKGYKMPGGEPYHPAGFMTFV
GASAMVHGKTLGVYPAAKALLSAVYEGAMVPFDTALKIEARWFTHVLMNPSSTAMIRSLFINKEALEKGANRPEAPDQSV
RKVGILGAGMMGAGIAYVSAMAGIKVVLIDSTQEAAERGKSYSTGLLDKAISRRKSTEEKKAEVLSRITATTDYAALAGC
DLIVEAVFEDPKVKAEVTKRAEAVIPQDAIFATNTSTLPISELAKASARPDQFIGIHFFSPVDKMLLVEIIKGRETGPRA
VAKALDFVRQIRKTPIVVNDARFFYANRCIIPYINEGIRMVAEGVSPTLIENAAKMMGMPLGPLQLVDETSIDLGVKIAK
ATKAAMGDAYPDGAVDAVIFKLADQGRLGRKTKAGFYDYDDAGKRQGFWSGLASEWPEADTQPDLTEIQHRLMFAQSLEA
VRALEEGVLEDIREGDVGAILGWGFAPWSGGPFGWLDMLGAPRAAGIADSLAEKFGPRFAAPQLLRDMAAKGESFYGRFG
AGKQAA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=735, Percent_Identity=33.0612244897959, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI68989263, Length=744, Percent_Identity=27.6881720430108, Blast_Score=248, Evalue=2e-65,
Organism=Homo sapiens, GI261878539, Length=626, Percent_Identity=28.4345047923323, Blast_Score=220, Evalue=4e-57,
Organism=Homo sapiens, GI296179429, Length=289, Percent_Identity=36.6782006920415, Blast_Score=168, Evalue=2e-41,
Organism=Homo sapiens, GI296179427, Length=306, Percent_Identity=34.640522875817, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI194097323, Length=204, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1788682, Length=724, Percent_Identity=35.6353591160221, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1790281, Length=726, Percent_Identity=33.0578512396694, Blast_Score=305, Evalue=8e-84,
Organism=Escherichia coli, GI1787661, Length=286, Percent_Identity=32.5174825174825, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787659, Length=200, Percent_Identity=33.5, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=738, Percent_Identity=31.4363143631436, Blast_Score=331, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17508951, Length=749, Percent_Identity=32.5767690253672, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17508953, Length=749, Percent_Identity=32.5767690253672, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI25144276, Length=627, Percent_Identity=32.8548644338118, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71985923, Length=370, Percent_Identity=30.8108108108108, Blast_Score=186, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI71985930, Length=367, Percent_Identity=29.4277929155313, Blast_Score=174, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17553560, Length=295, Percent_Identity=33.8983050847458, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17563036, Length=291, Percent_Identity=32.6460481099656, Blast_Score=142, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17549919, Length=292, Percent_Identity=30.8219178082192, Blast_Score=139, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=188, Percent_Identity=31.9148936170213, Blast_Score=73, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=198, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921000, Length=761, Percent_Identity=33.377135348226, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24583077, Length=757, Percent_Identity=33.553500660502, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24583079, Length=757, Percent_Identity=33.553500660502, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI20129971, Length=188, Percent_Identity=32.9787234042553, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24653477, Length=188, Percent_Identity=32.9787234042553, Blast_Score=83, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 77186; Mature: 77055

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFTMQKDADGVAIVTWDVPGKSMNVLSLDGAAELNALIDDALADEAVKGIVITSGKKD
CCCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCEEEEECCCCH
FAAGMDLNVIAGMRQGGAQAVFDGVMTLHHLLRKIELAGMDPKTKKGGKPIAAALPGTAL
HHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEE
GIGLELPLATHRIFAAENPKAKIGLPEIMVGIFPGAGGTTRLVRKLGAMGAAPFLLEGKL
EEEEECCHHHHEEEECCCCCCCCCCHHEEHEEECCCCCHHHHHHHHHCCCCCCEEEECCC
SDPARAKSAGLIDEVVADPVAAAREWVLKAADADLVKPWDAKGYKMPGGEPYHPAGFMTF
CCCHHHHHCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VGASAMVHGKTLGVYPAAKALLSAVYEGAMVPFDTALKIEARWFTHVLMNPSSTAMIRSL
HCCHHEEECCEECCCHHHHHHHHHHHCCCCCCCCCEEEEEHEEHHHHHCCCCHHHHHHHH
FINKEALEKGANRPEAPDQSVRKVGILGAGMMGAGIAYVSAMAGIKVVLIDSTQEAAERG
HHCHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHCC
KSYSTGLLDKAISRRKSTEEKKAEVLSRITATTDYAALAGCDLIVEAVFEDPKVKAEVTK
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHH
RAEAVIPQDAIFATNTSTLPISELAKASARPDQFIGIHFFSPVDKMLLVEIIKGRETGPR
HHHHCCCCCCEEECCCCCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCHH
AVAKALDFVRQIRKTPIVVNDARFFYANRCIIPYINEGIRMVAEGVSPTLIENAAKMMGM
HHHHHHHHHHHHHCCCEEEECCEEEEECCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PLGPLQLVDETSIDLGVKIAKATKAAMGDAYPDGAVDAVIFKLADQGRLGRKTKAGFYDY
CCCCHHHHCCCCCCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
DDAGKRQGFWSGLASEWPEADTQPDLTEIQHRLMFAQSLEAVRALEEGVLEDIREGDVGA
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ILGWGFAPWSGGPFGWLDMLGAPRAAGIADSLAEKFGPRFAAPQLLRDMAAKGESFYGRF
EEECCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
GAGKQAA
CCCCCCC
>Mature Secondary Structure 
TDFTMQKDADGVAIVTWDVPGKSMNVLSLDGAAELNALIDDALADEAVKGIVITSGKKD
CCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCEEEEECCCCH
FAAGMDLNVIAGMRQGGAQAVFDGVMTLHHLLRKIELAGMDPKTKKGGKPIAAALPGTAL
HHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEE
GIGLELPLATHRIFAAENPKAKIGLPEIMVGIFPGAGGTTRLVRKLGAMGAAPFLLEGKL
EEEEECCHHHHEEEECCCCCCCCCCHHEEHEEECCCCCHHHHHHHHHCCCCCCEEEECCC
SDPARAKSAGLIDEVVADPVAAAREWVLKAADADLVKPWDAKGYKMPGGEPYHPAGFMTF
CCCHHHHHCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VGASAMVHGKTLGVYPAAKALLSAVYEGAMVPFDTALKIEARWFTHVLMNPSSTAMIRSL
HCCHHEEECCEECCCHHHHHHHHHHHCCCCCCCCCEEEEEHEEHHHHHCCCCHHHHHHHH
FINKEALEKGANRPEAPDQSVRKVGILGAGMMGAGIAYVSAMAGIKVVLIDSTQEAAERG
HHCHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHCC
KSYSTGLLDKAISRRKSTEEKKAEVLSRITATTDYAALAGCDLIVEAVFEDPKVKAEVTK
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHH
RAEAVIPQDAIFATNTSTLPISELAKASARPDQFIGIHFFSPVDKMLLVEIIKGRETGPR
HHHHCCCCCCEEECCCCCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCHH
AVAKALDFVRQIRKTPIVVNDARFFYANRCIIPYINEGIRMVAEGVSPTLIENAAKMMGM
HHHHHHHHHHHHHCCCEEEECCEEEEECCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PLGPLQLVDETSIDLGVKIAKATKAAMGDAYPDGAVDAVIFKLADQGRLGRKTKAGFYDY
CCCCHHHHCCCCCCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
DDAGKRQGFWSGLASEWPEADTQPDLTEIQHRLMFAQSLEAVRALEEGVLEDIREGDVGA
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ILGWGFAPWSGGPFGWLDMLGAPRAAGIADSLAEKFGPRFAAPQLLRDMAAKGESFYGRF
EEECCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
GAGKQAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA