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The map label for this gene is fadA [H]

Identifier: 119385595

GI number: 119385595

Start: 35803

End: 37014

Strand: Direct

Name: fadA [H]

Synonym: Pden_2870

Alternate gene names: 119385595

Gene position: 35803-37014 (Clockwise)

Preceding gene: 119385594

Following gene: 119385596

Centisome position: 2.07

GC content: 67.41

Gene sequence:

>1212_bases
ATGACCGAAGCCTATATCTATGACGCCGCCCGCACCCCGCGCGGCAAGGGCCGCCCCGACGGCAGCCTGCACGAAGTCAC
CTCGGTTGCGCTTTCGGCCCGGCTGCTGAACGCCGTCAAGGAGCGCAACGGGCTCACAGGCCATGCCGTCGAGGACGTGA
TCTGGGGCAATGTCACCCAGGTCAAGGAACAGGGCGGCTGCCTCGCCCGCTCGGCAGTGCTGGAATCCGACCTGGACGAA
AGCATCCCCGGCCTCGCCATCAACCGCTTCTGCGCCAGCGGCATGGAGGCGGTGAACCTGGCCGCGAACCAGGTCAGGGG
CGGCGCCGGCCAGGCCTATATCGCCGGCGGCGTCGAGATGATGGGCCGCGTCGCCATGGGCAGCGACGGCGCCGCCATCG
CCGTGGACCCCAGCCTGACCTTCAAGACCTATTTCGTGCCGCAGGGCATCAGCGCCGACATCATCGCCACCGAATACGGC
TTTTCGCGCGAGGATGCCGACAGCCTGGCCGTCGAGAGCCAGCGCCGCGCCGCCATCGCCTGGGATGAGGACCGCTTCGC
CAGGTCCATCGTGCCGGTCAGGGACCAGAACGGCCTGACCATCCTCGACCGCGACGAATACATGCGCCCCGGCACCACGC
TGGAGGATCTGGCCAAGCTGAAGGCCAGCTTCAAGGAGATGGGCGAGACCATGCCCGGCTTCGACAAGGTGGCGATGCTG
AAATACCCGCATCTCGGCCGCATCGAGCACATCCACCATGCCGGCAACAGCTCGGGCATCGTGGACGGCGCCGCCGCCGT
GCTGATCGGCAATGCGGAATTCGGCAAGGCTTACGGGCTGAAACCCCGCGCCCGCATCCGCGCCACCGCCAAGATCGGCA
CCGATCCGACCATCATGCTGACCGGTCCGGTGCCGGTGACCGAAAAGATCCTGAAGGACAGCGGCATGGCGATCTCCGAC
ATCGACCTCTTCGAGGTGAACGAGGCCTTCGCCTCGGTCGTGTTGCGCTTCATGCAGGCCTTCCAGGTCGACCCCGGCGT
GGTCAACGTCAATGGCGGCGCCATCGCGCTGGGTCACCCGCTGGGCGCGACCGGCGCCATCATCATCGGCACGCTGCTCG
ACGAGCTGGAGCGCCAGGACAAGACCGTGGGCCTCGCCACGCTCTGCATCGCGTCCGGCATGGGCGCGGCCACCATCATC
GAGCGCGTCTGA

Upstream 100 bases:

>100_bases
TCGATGCCTGGCTGAGCCGCATTCAGGCGCTGCCCGGCTGGAAACACCCCTATGACCTGATGCCCGGCAATCCCGCGGAC
CGGGCCGCGAAGGAGGACGC

Downstream 100 bases:

>100_bases
GGGAGGGGAACATGACCGATTTCACCATGCAGAAAGACGCCGACGGCGTCGCGATCGTCACCTGGGACGTGCCGGGCAAG
TCGATGAACGTGCTGTCGCT

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTEAYIYDAARTPRGKGRPDGSLHEVTSVALSARLLNAVKERNGLTGHAVEDVIWGNVTQVKEQGGCLARSAVLESDLDE
SIPGLAINRFCASGMEAVNLAANQVRGGAGQAYIAGGVEMMGRVAMGSDGAAIAVDPSLTFKTYFVPQGISADIIATEYG
FSREDADSLAVESQRRAAIAWDEDRFARSIVPVRDQNGLTILDRDEYMRPGTTLEDLAKLKASFKEMGETMPGFDKVAML
KYPHLGRIEHIHHAGNSSGIVDGAAAVLIGNAEFGKAYGLKPRARIRATAKIGTDPTIMLTGPVPVTEKILKDSGMAISD
IDLFEVNEAFASVVLRFMQAFQVDPGVVNVNGGAIALGHPLGATGAIIIGTLLDELERQDKTVGLATLCIASGMGAATII
ERV

Sequences:

>Translated_403_residues
MTEAYIYDAARTPRGKGRPDGSLHEVTSVALSARLLNAVKERNGLTGHAVEDVIWGNVTQVKEQGGCLARSAVLESDLDE
SIPGLAINRFCASGMEAVNLAANQVRGGAGQAYIAGGVEMMGRVAMGSDGAAIAVDPSLTFKTYFVPQGISADIIATEYG
FSREDADSLAVESQRRAAIAWDEDRFARSIVPVRDQNGLTILDRDEYMRPGTTLEDLAKLKASFKEMGETMPGFDKVAML
KYPHLGRIEHIHHAGNSSGIVDGAAAVLIGNAEFGKAYGLKPRARIRATAKIGTDPTIMLTGPVPVTEKILKDSGMAISD
IDLFEVNEAFASVVLRFMQAFQVDPGVVNVNGGAIALGHPLGATGAIIIGTLLDELERQDKTVGLATLCIASGMGAATII
ERV
>Mature_402_residues
TEAYIYDAARTPRGKGRPDGSLHEVTSVALSARLLNAVKERNGLTGHAVEDVIWGNVTQVKEQGGCLARSAVLESDLDES
IPGLAINRFCASGMEAVNLAANQVRGGAGQAYIAGGVEMMGRVAMGSDGAAIAVDPSLTFKTYFVPQGISADIIATEYGF
SREDADSLAVESQRRAAIAWDEDRFARSIVPVRDQNGLTILDRDEYMRPGTTLEDLAKLKASFKEMGETMPGFDKVAMLK
YPHLGRIEHIHHAGNSSGIVDGAAAVLIGNAEFGKAYGLKPRARIRATAKIGTDPTIMLTGPVPVTEKILKDSGMAISDI
DLFEVNEAFASVVLRFMQAFQVDPGVVNVNGGAIALGHPLGATGAIIIGTLLDELERQDKTVGLATLCIASGMGAATIIE
RV

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=390, Percent_Identity=35.8974358974359, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI167614485, Length=411, Percent_Identity=33.8199513381995, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI148539872, Length=415, Percent_Identity=34.2168674698795, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI4557237, Length=417, Percent_Identity=30.9352517985612, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4504327, Length=447, Percent_Identity=27.7404921700224, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI194353979, Length=121, Percent_Identity=42.1487603305785, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI48994986, Length=413, Percent_Identity=39.9515738498789, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1787663, Length=427, Percent_Identity=38.8758782201405, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788554, Length=418, Percent_Identity=38.755980861244, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87082165, Length=418, Percent_Identity=37.0813397129187, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1788683, Length=442, Percent_Identity=28.2805429864253, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI133906874, Length=413, Percent_Identity=33.8983050847458, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI25147385, Length=415, Percent_Identity=32.289156626506, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17535921, Length=417, Percent_Identity=30.4556354916067, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=29.8765432098765, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17551802, Length=448, Percent_Identity=27.0089285714286, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=33.4158415841584, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325229, Length=421, Percent_Identity=30.166270783848, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24655093, Length=417, Percent_Identity=37.6498800959233, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI17648125, Length=414, Percent_Identity=35.5072463768116, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24640423, Length=417, Percent_Identity=30.2158273381295, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI17137578, Length=446, Percent_Identity=26.0089686098655, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42627; Mature: 42496

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAYIYDAARTPRGKGRPDGSLHEVTSVALSARLLNAVKERNGLTGHAVEDVIWGNVTQ
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHH
VKEQGGCLARSAVLESDLDESIPGLAINRFCASGMEAVNLAANQVRGGAGQAYIAGGVEM
HHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCHHH
MGRVAMGSDGAAIAVDPSLTFKTYFVPQGISADIIATEYGFSREDADSLAVESQRRAAIA
HHHHEECCCCCEEEECCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEE
WDEDRFARSIVPVRDQNGLTILDRDEYMRPGTTLEDLAKLKASFKEMGETMPGFDKVAML
ECHHHHHHHCCCEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
KYPHLGRIEHIHHAGNSSGIVDGAAAVLIGNAEFGKAYGLKPRARIRATAKIGTDPTIML
CCCCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHEEEEEECCCCCEEEE
TGPVPVTEKILKDSGMAISDIDLFEVNEAFASVVLRFMQAFQVDPGVVNVNGGAIALGHP
ECCCCHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCC
LGATGAIIIGTLLDELERQDKTVGLATLCIASGMGAATIIERV
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TEAYIYDAARTPRGKGRPDGSLHEVTSVALSARLLNAVKERNGLTGHAVEDVIWGNVTQ
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHH
VKEQGGCLARSAVLESDLDESIPGLAINRFCASGMEAVNLAANQVRGGAGQAYIAGGVEM
HHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCHHH
MGRVAMGSDGAAIAVDPSLTFKTYFVPQGISADIIATEYGFSREDADSLAVESQRRAAIA
HHHHEECCCCCEEEECCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEE
WDEDRFARSIVPVRDQNGLTILDRDEYMRPGTTLEDLAKLKASFKEMGETMPGFDKVAML
ECHHHHHHHCCCEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
KYPHLGRIEHIHHAGNSSGIVDGAAAVLIGNAEFGKAYGLKPRARIRATAKIGTDPTIML
CCCCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHEEEEEECCCCCEEEE
TGPVPVTEKILKDSGMAISDIDLFEVNEAFASVVLRFMQAFQVDPGVVNVNGGAIALGHP
ECCCCHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCC
LGATGAIIIGTLLDELERQDKTVGLATLCIASGMGAATIIERV
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]