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The map label for this gene is liaS [H]

Identifier: 119358451

GI number: 119358451

Start: 3080957

End: 3082621

Strand: Reverse

Name: liaS [H]

Synonym: Cpha266_2687

Alternate gene names: 119358451

Gene position: 3082621-3080957 (Counterclockwise)

Preceding gene: 119358452

Following gene: 119358450

Centisome position: 98.36

GC content: 51.05

Gene sequence:

>1665_bases
ATGAAAAAAAACAGAACAGCAGATGCCGACGACTTCTCCGAAAAGCGTCTCCAGGCAGAAGCGATCCTGCTGCATGAGCG
AAAAAAAAAAGTTGATAGCCTTGAATCAGTCGAAGATCGTCTTCGCATTATCCATGAGCTATCGGTCAACCAGATTGAGC
TTGAAATGCAGCAGGACGAACTGCTGCAGTCGAGAGCAGTTCTCGAAGCGGGGTTAAAGAGATATAACGAGCTCTACGAT
TTTGCGCCCCTTGGATACCTGACCATCGCCGCGGACAGCACAATACGCAAGCTGAACCTGACGGCCGCAACAATGCTTGG
TCTCGATCGCTCTCTTTTGAAAGGCGACCGGTTGGGACGGTTTATTGTCTATGAAGATCTTCCGGTTTTCAATGCCCTCA
TAAAGAGAGTCTTCGCCACTCGGGAGAGCGGATATTGCGAGGTGATGCTGCTTGACCATGCCAGAGATAAACAGGAGATC
GCAACCTCCGGCCCGCGTCAACGACGCATGGTTCGTATCGATGCCATAGTTAACAGCGAAAAACAGGAGTGCTGGGCCTT
TCTGACGGACATCACCATTCAAAAACAGCTTGAGGACTCGTTATGGGAAAGCGACCGCCTCTATCGATGCCTGATTGAAA
CGGTCAGCGAAGGCGTTCTTGTTATTCACGGCGACCATTTGCGTTTTGTGAATCCGATCGTATCGGAAATGACCGGTTAC
ACTGAAGCGGAGCTGCTCTCCTTTTCGTTTACCGATATGATGCATCCTGATGACAGGGAGCGGGTAAAACACCACCACCT
CAAATGTCTCAAGAGCGACCTGCCCGACCTGAGAATTGAGTTACGGATCATCAAAAAAAACGGAAGGATCCTCTGGATCG
AAATGGGCGGGTTAAAAACCGAATGGAACGGCAAGCCCGCAATGCTATACGTTTTGATAGACATCACCGAGCGAAAAGTC
CTTGAGGAGAAGCTGCAAACCGAAAAGCAGCAGCTTCTTGATACGCTCAGAACCACCGATCAATACCAGGCTCAACTGCA
GGAGCTCAACAGCAAAATCAAGGTCATGTCGGAAGTCGAGGAGCGCTCCCTGCTCTACCGTGACCTGCACGACGGAGCAG
GCCAGTCGCTGCACGCGGTATGTCTGCATCTTAAAATGATTGCGGATGGTCGCGGAGGATATGGAGACCTCAAGTCGCTC
GCATCTGAACTTGCTGGTGAAATTGCCGATATCTCTGCTGAAATCCGTGATATTGCCCATCACCTCCGTCCTGCCTATCT
TCAGGAAATCACCCTTGATCGAGCCATTATCAAGCGCTGCGAGATGCTCGGAAGACGAGGGGTTCCAATCAGCATCAGTT
GTGTCGGCGATTTCAGTTCCCTCTCCTGTCAGGTCAGCGAAAACCTCTACCGTATTTCCCAGGAGGCGATAGCAAATGCC
GACCGCCACGCGGCGGCAACCCTGATCACGGTACGCTTAACCCGTGTTGATAATGCGTTAACACTGCTCATAGCCGATAA
TGGTTGCGGAATAAAAGACGTTTCGACAAATAAAGGTGTTGGACTGCGAATCATAGAGGAACGGGTTTCGCTCATAGGCG
GAAAGCTCGACATGGCATCCACCGCTTCGGGCACCACAATTACCGTGACGCTGGAGTTGCCATGA

Upstream 100 bases:

>100_bases
GAGGCCTCCCGGGCCTGTTTATCAGGTTGAGGGACGTAGCGCACGTTTTGGCAGTACTATCGGAACAGGGTGCTTTCGCT
TAATCAGGAGAACTCACTTC

Downstream 100 bases:

>100_bases
TTACCATTATCATAGCCGACGACCACAAAATATTCCGACAGGGATTATCGAGGCTTCTTGCCGATGACGACAGGTTCAAG
CTTCTTGGCGAGGCAAGTAA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKKNRTADADDFSEKRLQAEAILLHERKKKVDSLESVEDRLRIIHELSVNQIELEMQQDELLQSRAVLEAGLKRYNELYD
FAPLGYLTIAADSTIRKLNLTAATMLGLDRSLLKGDRLGRFIVYEDLPVFNALIKRVFATRESGYCEVMLLDHARDKQEI
ATSGPRQRRMVRIDAIVNSEKQECWAFLTDITIQKQLEDSLWESDRLYRCLIETVSEGVLVIHGDHLRFVNPIVSEMTGY
TEAELLSFSFTDMMHPDDRERVKHHHLKCLKSDLPDLRIELRIIKKNGRILWIEMGGLKTEWNGKPAMLYVLIDITERKV
LEEKLQTEKQQLLDTLRTTDQYQAQLQELNSKIKVMSEVEERSLLYRDLHDGAGQSLHAVCLHLKMIADGRGGYGDLKSL
ASELAGEIADISAEIRDIAHHLRPAYLQEITLDRAIIKRCEMLGRRGVPISISCVGDFSSLSCQVSENLYRISQEAIANA
DRHAAATLITVRLTRVDNALTLLIADNGCGIKDVSTNKGVGLRIIEERVSLIGGKLDMASTASGTTITVTLELP

Sequences:

>Translated_554_residues
MKKNRTADADDFSEKRLQAEAILLHERKKKVDSLESVEDRLRIIHELSVNQIELEMQQDELLQSRAVLEAGLKRYNELYD
FAPLGYLTIAADSTIRKLNLTAATMLGLDRSLLKGDRLGRFIVYEDLPVFNALIKRVFATRESGYCEVMLLDHARDKQEI
ATSGPRQRRMVRIDAIVNSEKQECWAFLTDITIQKQLEDSLWESDRLYRCLIETVSEGVLVIHGDHLRFVNPIVSEMTGY
TEAELLSFSFTDMMHPDDRERVKHHHLKCLKSDLPDLRIELRIIKKNGRILWIEMGGLKTEWNGKPAMLYVLIDITERKV
LEEKLQTEKQQLLDTLRTTDQYQAQLQELNSKIKVMSEVEERSLLYRDLHDGAGQSLHAVCLHLKMIADGRGGYGDLKSL
ASELAGEIADISAEIRDIAHHLRPAYLQEITLDRAIIKRCEMLGRRGVPISISCVGDFSSLSCQVSENLYRISQEAIANA
DRHAAATLITVRLTRVDNALTLLIADNGCGIKDVSTNKGVGLRIIEERVSLIGGKLDMASTASGTTITVTLELP
>Mature_554_residues
MKKNRTADADDFSEKRLQAEAILLHERKKKVDSLESVEDRLRIIHELSVNQIELEMQQDELLQSRAVLEAGLKRYNELYD
FAPLGYLTIAADSTIRKLNLTAATMLGLDRSLLKGDRLGRFIVYEDLPVFNALIKRVFATRESGYCEVMLLDHARDKQEI
ATSGPRQRRMVRIDAIVNSEKQECWAFLTDITIQKQLEDSLWESDRLYRCLIETVSEGVLVIHGDHLRFVNPIVSEMTGY
TEAELLSFSFTDMMHPDDRERVKHHHLKCLKSDLPDLRIELRIIKKNGRILWIEMGGLKTEWNGKPAMLYVLIDITERKV
LEEKLQTEKQQLLDTLRTTDQYQAQLQELNSKIKVMSEVEERSLLYRDLHDGAGQSLHAVCLHLKMIADGRGGYGDLKSL
ASELAGEIADISAEIRDIAHHLRPAYLQEITLDRAIIKRCEMLGRRGVPISISCVGDFSSLSCQVSENLYRISQEAIANA
DRHAAATLITVRLTRVDNALTLLIADNGCGIKDVSTNKGVGLRIIEERVSLIGGKLDMASTASGTTITVTLELP

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4564

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=238, Percent_Identity=27.3109243697479, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788812, Length=193, Percent_Identity=27.4611398963731, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082311, Length=127, Percent_Identity=33.8582677165354, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62689; Mature: 62689

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRTADADDFSEKRLQAEAILLHERKKKVDSLESVEDRLRIIHELSVNQIELEMQQDE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHH
LLQSRAVLEAGLKRYNELYDFAPLGYLTIAADSTIRKLNLTAATMLGLDRSLLKGDRLGR
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHEEEHHHHHHHHCCHHHHCCCCCCC
FIVYEDLPVFNALIKRVFATRESGYCEVMLLDHARDKQEIATSGPRQRRMVRIDAIVNSE
EEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCHHHHEEEEEHHHCCC
KQECWAFLTDITIQKQLEDSLWESDRLYRCLIETVSEGVLVIHGDHLRFVNPIVSEMTGY
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCC
TEAELLSFSFTDMMHPDDRERVKHHHLKCLKSDLPDLRIELRIIKKNGRILWIEMGGLKT
CHHHHHEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCEEE
EWNGKPAMLYVLIDITERKVLEEKLQTEKQQLLDTLRTTDQYQAQLQELNSKIKVMSEVE
CCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ERSLLYRDLHDGAGQSLHAVCLHLKMIADGRGGYGDLKSLASELAGEIADISAEIRDIAH
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLRPAYLQEITLDRAIIKRCEMLGRRGVPISISCVGDFSSLSCQVSENLYRISQEAIANA
HHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHCCC
DRHAAATLITVRLTRVDNALTLLIADNGCGIKDVSTNKGVGLRIIEERVSLIGGKLDMAS
CHHHHHEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCC
TASGTTITVTLELP
CCCCCEEEEEEECC
>Mature Secondary Structure
MKKNRTADADDFSEKRLQAEAILLHERKKKVDSLESVEDRLRIIHELSVNQIELEMQQDE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHH
LLQSRAVLEAGLKRYNELYDFAPLGYLTIAADSTIRKLNLTAATMLGLDRSLLKGDRLGR
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHEEEHHHHHHHHCCHHHHCCCCCCC
FIVYEDLPVFNALIKRVFATRESGYCEVMLLDHARDKQEIATSGPRQRRMVRIDAIVNSE
EEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCHHHHEEEEEHHHCCC
KQECWAFLTDITIQKQLEDSLWESDRLYRCLIETVSEGVLVIHGDHLRFVNPIVSEMTGY
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCC
TEAELLSFSFTDMMHPDDRERVKHHHLKCLKSDLPDLRIELRIIKKNGRILWIEMGGLKT
CHHHHHEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCEEE
EWNGKPAMLYVLIDITERKVLEEKLQTEKQQLLDTLRTTDQYQAQLQELNSKIKVMSEVE
CCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ERSLLYRDLHDGAGQSLHAVCLHLKMIADGRGGYGDLKSLASELAGEIADISAEIRDIAH
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLRPAYLQEITLDRAIIKRCEMLGRRGVPISISCVGDFSSLSCQVSENLYRISQEAIANA
HHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHCCC
DRHAAATLITVRLTRVDNALTLLIADNGCGIKDVSTNKGVGLRIIEERVSLIGGKLDMAS
CHHHHHEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCC
TASGTTITVTLELP
CCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]