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The map label for this gene is leuA2 [H]

Identifier: 119357714

GI number: 119357714

Start: 2225309

End: 2226466

Strand: Direct

Name: leuA2 [H]

Synonym: Cpha266_1918

Alternate gene names: 119357714

Gene position: 2225309-2226466 (Clockwise)

Preceding gene: 119357713

Following gene: 119357715

Centisome position: 71.01

GC content: 51.73

Gene sequence:

>1158_bases
ATGATACTTGAAAAGAATACGCCTTCGGGCAATGGCATAAGGCCCTGGATTATCGACACAACCCTGAGAGATGGCGAACA
GGCTCCGGGAGTTGTATTTACTGCTGGCGAAAAATACAGAATCGCTCAACTGCTTGCAGAAATTGGCGTCAACGAGCTTG
AAATCGGGTATCCGGCAATCAGCAGCGAGGAACGAGAGAACATCAGAACCATTGCCGCGCTTCATCTGCCGGTGCGCCTG
ACCAGTTGGGCAAGAGCGTCATGGGACGATATCGAACATGCCAGGAGCTGCGAAACCGAGGCGGTGCACATCAGTTTTCC
GGTCTCGCCACTCTACCTGCAACTGATGCAGAAGGATTACCTGTGGGTGCAGCGCCAGCTCCAGGAACTGGTGCCGAAAG
CAAAAAAATATTTCAACATTGTCAGTGTCGGGGCTCAGGATGCAACAAGAACGCCGTATGAACTTCTGAAAACCTTTGTT
CTCGATGCTGAAGCGTGCGGAGCCGACAGGATTCGCATAGCCGATACCGTTGGCATAGCGACTCCCATATCGGTACTCGA
TCTGGTGGGACGTCTTCAGTCCGTAAGCCCGACAGCCCTCGAATTTCATGCGCACAACGATCTCGGCATGGCAACGGCCA
ATGCGTTCACCGCTCTTGAGGCCGGCTGCTCCGCTGTGAGCGTTTCGGTAACGGGACTTGGCGAACGGGCGGGTAACGCC
GCTCTTGAAGAACTTGCCGTCGCTCTTTTGCTCAACAATCAATTCCAATGCAAGATCGACACCACAAAGCTTGCTATGCT
CTGTAAAACCGTCAGCAAAGCATCCGGAAGACCAATCCAGGATCAGAAACCGGTTATCGGCAAATCGGTATTCCAGCACG
AATCAGGCATTCATTGCGCAGCATTGTTAAAAAATCCGCTCTCCTACCAACCATTTCTTCCATCTGAAGTCGGAAGAAAA
CCTCATGAGCTGGTAATCGGCAAGCATTCCGGCAGTGCGGCGCTCAAACATTTTTACCATACAAGAGGAATCAGCCTGAC
AAGAGATGAAGCCAGCCGGATCCTGAGTCTGGTCCGCAGAAGCGCTGATGAAAAGAAAAGAGCACTGACAGCCCATGAAC
TTGATGAGATCTACGCAATACGCAGCCAAAAAGGATAA

Upstream 100 bases:

>100_bases
TCCTAATTTATTTAGGTTATATATATTTCATTCCTAAATATTCATATGGTAACCTGAACGCAATCATGCCGCCTGATGTC
AGGCAATAAATGGACGTTTC

Downstream 100 bases:

>100_bases
CCATTTCGCAGGATCCATGCTCATACCACAGCAAAAAGAACTGAACAGCATAAGCCTGCTGGCTGAAGTCAGCAGAACGG
TAACCAACGAAAACGATATC

Product: homocitrate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MILEKNTPSGNGIRPWIIDTTLRDGEQAPGVVFTAGEKYRIAQLLAEIGVNELEIGYPAISSEERENIRTIAALHLPVRL
TSWARASWDDIEHARSCETEAVHISFPVSPLYLQLMQKDYLWVQRQLQELVPKAKKYFNIVSVGAQDATRTPYELLKTFV
LDAEACGADRIRIADTVGIATPISVLDLVGRLQSVSPTALEFHAHNDLGMATANAFTALEAGCSAVSVSVTGLGERAGNA
ALEELAVALLLNNQFQCKIDTTKLAMLCKTVSKASGRPIQDQKPVIGKSVFQHESGIHCAALLKNPLSYQPFLPSEVGRK
PHELVIGKHSGSAALKHFYHTRGISLTRDEASRILSLVRRSADEKKRALTAHELDEIYAIRSQKG

Sequences:

>Translated_385_residues
MILEKNTPSGNGIRPWIIDTTLRDGEQAPGVVFTAGEKYRIAQLLAEIGVNELEIGYPAISSEERENIRTIAALHLPVRL
TSWARASWDDIEHARSCETEAVHISFPVSPLYLQLMQKDYLWVQRQLQELVPKAKKYFNIVSVGAQDATRTPYELLKTFV
LDAEACGADRIRIADTVGIATPISVLDLVGRLQSVSPTALEFHAHNDLGMATANAFTALEAGCSAVSVSVTGLGERAGNA
ALEELAVALLLNNQFQCKIDTTKLAMLCKTVSKASGRPIQDQKPVIGKSVFQHESGIHCAALLKNPLSYQPFLPSEVGRK
PHELVIGKHSGSAALKHFYHTRGISLTRDEASRILSLVRRSADEKKRALTAHELDEIYAIRSQKG
>Mature_385_residues
MILEKNTPSGNGIRPWIIDTTLRDGEQAPGVVFTAGEKYRIAQLLAEIGVNELEIGYPAISSEERENIRTIAALHLPVRL
TSWARASWDDIEHARSCETEAVHISFPVSPLYLQLMQKDYLWVQRQLQELVPKAKKYFNIVSVGAQDATRTPYELLKTFV
LDAEACGADRIRIADTVGIATPISVLDLVGRLQSVSPTALEFHAHNDLGMATANAFTALEAGCSAVSVSVTGLGERAGNA
ALEELAVALLLNNQFQCKIDTTKLAMLCKTVSKASGRPIQDQKPVIGKSVFQHESGIHCAALLKNPLSYQPFLPSEVGRK
PHELVIGKHSGSAALKHFYHTRGISLTRDEASRILSLVRRSADEKKRALTAHELDEIYAIRSQKG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=383, Percent_Identity=30.5483028720627, Blast_Score=168, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6320019, Length=364, Percent_Identity=29.3956043956044, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320071, Length=364, Percent_Identity=29.3956043956044, Blast_Score=160, Evalue=5e-40,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42194; Mature: 42194

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILEKNTPSGNGIRPWIIDTTLRDGEQAPGVVFTAGEKYRIAQLLAEIGVNELEIGYPAI
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEECCCCCC
SSEERENIRTIAALHLPVRLTSWARASWDDIEHARSCETEAVHISFPVSPLYLQLMQKDY
CCHHHHHHHHHHEEECCEEECHHHCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
LWVQRQLQELVPKAKKYFNIVSVGAQDATRTPYELLKTFVLDAEACGADRIRIADTVGIA
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCHHHCCCCCEEEEHHHCCC
TPISVLDLVGRLQSVSPTALEFHAHNDLGMATANAFTALEAGCSAVSVSVTGLGERAGNA
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
ALEELAVALLLNNQFQCKIDTTKLAMLCKTVSKASGRPIQDQKPVIGKSVFQHESGIHCA
HHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHH
ALLKNPLSYQPFLPSEVGRKPHELVIGKHSGSAALKHFYHTRGISLTRDEASRILSLVRR
HHHCCCCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
SADEKKRALTAHELDEIYAIRSQKG
CHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILEKNTPSGNGIRPWIIDTTLRDGEQAPGVVFTAGEKYRIAQLLAEIGVNELEIGYPAI
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEECCCCCC
SSEERENIRTIAALHLPVRLTSWARASWDDIEHARSCETEAVHISFPVSPLYLQLMQKDY
CCHHHHHHHHHHEEECCEEECHHHCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
LWVQRQLQELVPKAKKYFNIVSVGAQDATRTPYELLKTFVLDAEACGADRIRIADTVGIA
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCHHHCCCCCEEEEHHHCCC
TPISVLDLVGRLQSVSPTALEFHAHNDLGMATANAFTALEAGCSAVSVSVTGLGERAGNA
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
ALEELAVALLLNNQFQCKIDTTKLAMLCKTVSKASGRPIQDQKPVIGKSVFQHESGIHCA
HHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHH
ALLKNPLSYQPFLPSEVGRKPHELVIGKHSGSAALKHFYHTRGISLTRDEASRILSLVRR
HHHCCCCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
SADEKKRALTAHELDEIYAIRSQKG
CHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]