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The map label for this gene is vnfA [H]

Identifier: 119357715

GI number: 119357715

Start: 2226483

End: 2228120

Strand: Direct

Name: vnfA [H]

Synonym: Cpha266_1919

Alternate gene names: 119357715

Gene position: 2226483-2228120 (Clockwise)

Preceding gene: 119357714

Following gene: 119357721

Centisome position: 71.05

GC content: 48.6

Gene sequence:

>1638_bases
ATGCTCATACCACAGCAAAAAGAACTGAACAGCATAAGCCTGCTGGCTGAAGTCAGCAGAACGGTAACCAACGAAAACGA
TATCAGTAAAGTACTGCGGATCGTTCTGTTCATCATGTCGGAACACCTCGATATGCTCAGGGGCATGATCACCATTCTCA
ATCGAGAAACAGGAGAAATAACCACCAACGAATCGTTCGGCTTGACCGAAGAAGAGATGGAGCGAGGCAGATACCGGATT
GGCGAAGGAATTATCGGGCAGGTGGTAAAAACAGGAAAACCTTTCATTGTACCCAACATCGGTGATGAGCCGCTTTTTCT
CGACCGTACCCGTTCACGACAGAAAACAAAAAAAGAGAACCTCTGCTTTATCTGCATTCCCATCAGATCAGGAGCTGAAA
TAATCGGCACCCTCAGTGCTGACCGGCAGCTTGAGCCCTCACTGTTGAAGGAATCGGGAAAAAGAATGAAAGCCGAGGAT
GAACGAATGGATCTTTTGCAGCACTATGTCGATCTTCTCTCCATTATCGCATCGATGATATCGCAGGCCGTCAGACTCAA
ACAGATAGCCCATGAAGCAACGGCAGGCATTGCAGTAGTGGAACACAAACCTCAGCAGGAAAAGAAAGCGGGATTTATGC
TCGGCAGCAAGGATGCCGGAGAGGAGAGCATTGCTGAAAGCGAACGTCCGGCAAACATAATCGGCAACTCGAAACCGATG
TTATCGCTTTTCAGGATGATCGACAAAATCTCAAAAACAAATGCCACAACGCTTGTGCTTGGAGAGAGCGGGGTTGGCAA
AGAGCTTGTCGCCAGCGCCATCCACTTTAAAAGCCGCAGGGAAGGAAATCCATTTATCAAGTTCAACTGTGCAGCTCTGC
CTGAAAGCATTGTTGAAAGCGAGCTTTTCGGTCATGAAAAAGGTTCGTTTACTGGCGCTTCAGCCACCCGAAAAGGTCGT
TTCGAACTTGCGCATACCGGCACGATCTTTCTGGATGAAGTCGGCGAACTCAGCCTGCCCATCCAGGCTAAACTGCTTCG
AATCATTCAGGAAAAAGAGTTTGAACGGGTTGGTGGTTCAAAAACCATCAAGGTTGATGTCCGTGTGATTGCCGCTACAA
ACAGAAACCTTGAAACGCTTATTCGCGAAGGGCTTTTCAGGGAGGATCTCTTCTACCGACTCAATATCTTTCCGATAACC
GTGCCGCCTCTGAGAGAACGAAAAACGGACATTCTTCTCCTTGCCGACTATTTTGTTGAAAAATACAATGTAACCAACCA
GAAAGGAATCCGCAGAATTTCGACAACGGCTATTGACATGCTGATGCGCTATCACTGGCCGGGCAACGTCCGGGAACTGG
AAAACTGCATCGAGCGGGCCGTGATTCTCAGCGAAGACAACGTTATACACGGCTACCACCTGCCTCCGAGCCTGCAAACC
GCAGAGTCAAGCGGCACTCCCTATACTGGCTCGATGATGCAGAAACTCGATACCATTGAGAAGGAAATGATCATCGAAGC
TTTGAAACGGACTAAAGGAAACATGTCGCGCGCAGCGGCACAACTTGGCCTTTCAGACCGGATCATGGGACTACGGGTAA
AAAAGTTCGATATCGATTACCGGATATTCCGACCCTGA

Upstream 100 bases:

>100_bases
CGCAGAAGCGCTGATGAAAAGAAAAGAGCACTGACAGCCCATGAACTTGATGAGATCTACGCAATACGCAGCCAAAAAGG
ATAACCATTTCGCAGGATCC

Downstream 100 bases:

>100_bases
AAAGGCGCGCTCTGCCTATGCGTACATCGTGGCAATAAACGACTCTATCCGAGAGGCATCCTCATCTGATAGGAGAAACT
CCCCTGCCCCGATCACACCC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MLIPQQKELNSISLLAEVSRTVTNENDISKVLRIVLFIMSEHLDMLRGMITILNRETGEITTNESFGLTEEEMERGRYRI
GEGIIGQVVKTGKPFIVPNIGDEPLFLDRTRSRQKTKKENLCFICIPIRSGAEIIGTLSADRQLEPSLLKESGKRMKAED
ERMDLLQHYVDLLSIIASMISQAVRLKQIAHEATAGIAVVEHKPQQEKKAGFMLGSKDAGEESIAESERPANIIGNSKPM
LSLFRMIDKISKTNATTLVLGESGVGKELVASAIHFKSRREGNPFIKFNCAALPESIVESELFGHEKGSFTGASATRKGR
FELAHTGTIFLDEVGELSLPIQAKLLRIIQEKEFERVGGSKTIKVDVRVIAATNRNLETLIREGLFREDLFYRLNIFPIT
VPPLRERKTDILLLADYFVEKYNVTNQKGIRRISTTAIDMLMRYHWPGNVRELENCIERAVILSEDNVIHGYHLPPSLQT
AESSGTPYTGSMMQKLDTIEKEMIIEALKRTKGNMSRAAAQLGLSDRIMGLRVKKFDIDYRIFRP

Sequences:

>Translated_545_residues
MLIPQQKELNSISLLAEVSRTVTNENDISKVLRIVLFIMSEHLDMLRGMITILNRETGEITTNESFGLTEEEMERGRYRI
GEGIIGQVVKTGKPFIVPNIGDEPLFLDRTRSRQKTKKENLCFICIPIRSGAEIIGTLSADRQLEPSLLKESGKRMKAED
ERMDLLQHYVDLLSIIASMISQAVRLKQIAHEATAGIAVVEHKPQQEKKAGFMLGSKDAGEESIAESERPANIIGNSKPM
LSLFRMIDKISKTNATTLVLGESGVGKELVASAIHFKSRREGNPFIKFNCAALPESIVESELFGHEKGSFTGASATRKGR
FELAHTGTIFLDEVGELSLPIQAKLLRIIQEKEFERVGGSKTIKVDVRVIAATNRNLETLIREGLFREDLFYRLNIFPIT
VPPLRERKTDILLLADYFVEKYNVTNQKGIRRISTTAIDMLMRYHWPGNVRELENCIERAVILSEDNVIHGYHLPPSLQT
AESSGTPYTGSMMQKLDTIEKEMIIEALKRTKGNMSRAAAQLGLSDRIMGLRVKKFDIDYRIFRP
>Mature_545_residues
MLIPQQKELNSISLLAEVSRTVTNENDISKVLRIVLFIMSEHLDMLRGMITILNRETGEITTNESFGLTEEEMERGRYRI
GEGIIGQVVKTGKPFIVPNIGDEPLFLDRTRSRQKTKKENLCFICIPIRSGAEIIGTLSADRQLEPSLLKESGKRMKAED
ERMDLLQHYVDLLSIIASMISQAVRLKQIAHEATAGIAVVEHKPQQEKKAGFMLGSKDAGEESIAESERPANIIGNSKPM
LSLFRMIDKISKTNATTLVLGESGVGKELVASAIHFKSRREGNPFIKFNCAALPESIVESELFGHEKGSFTGASATRKGR
FELAHTGTIFLDEVGELSLPIQAKLLRIIQEKEFERVGGSKTIKVDVRVIAATNRNLETLIREGLFREDLFYRLNIFPIT
VPPLRERKTDILLLADYFVEKYNVTNQKGIRRISTTAIDMLMRYHWPGNVRELENCIERAVILSEDNVIHGYHLPPSLQT
AESSGTPYTGSMMQKLDTIEKEMIIEALKRTKGNMSRAAAQLGLSDRIMGLRVKKFDIDYRIFRP

Specific function: Required for the expression of the V-dependent nitrogen fixation system in Azotobacter vinelandii. It is required for the regulation of nitrogenase 2 transcription. Interacts with sigma- 54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=324, Percent_Identity=48.1481481481481, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI87082117, Length=560, Percent_Identity=35.3571428571429, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=46.6237942122186, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1789087, Length=310, Percent_Identity=46.4516129032258, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI87082152, Length=333, Percent_Identity=43.8438438438438, Blast_Score=264, Evalue=9e-72,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=43.5736677115987, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790299, Length=333, Percent_Identity=38.7387387387387, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788905, Length=308, Percent_Identity=40.5844155844156, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=38.2716049382716, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=40.7975460122699, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=36.9085173501577, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=32.6732673267327, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=37.9310344827586, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 61316; Mature: 61316

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIPQQKELNSISLLAEVSRTVTNENDISKVLRIVLFIMSEHLDMLRGMITILNRETGEI
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
TTNESFGLTEEEMERGRYRIGEGIIGQVVKTGKPFIVPNIGDEPLFLDRTRSRQKTKKEN
ECCCCCCCCHHHHHCCHHHHCCHHHHHHHHCCCCEEECCCCCCCEEECCHHHHHHHHHCC
LCFICIPIRSGAEIIGTLSADRQLEPSLLKESGKRMKAEDERMDLLQHYVDLLSIIASMI
EEEEEEECCCCCCEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SQAVRLKQIAHEATAGIAVVEHKPQQEKKAGFMLGSKDAGEESIAESERPANIIGNSKPM
HHHHHHHHHHHHHHCCEEEEECCCCHHHHCCCEECCCCCCHHHHHHCCCCCHHCCCCCHH
LSLFRMIDKISKTNATTLVLGESGVGKELVASAIHFKSRREGNPFIKFNCAALPESIVES
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHH
ELFGHEKGSFTGASATRKGRFELAHTGTIFLDEVGELSLPIQAKLLRIIQEKEFERVGGS
HHHCCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
KTIKVDVRVIAATNRNLETLIREGLFREDLFYRLNIFPITVPPLRERKTDILLLADYFVE
EEEEEEEEEEEECCCCHHHHHHCCCHHHCCEEEEEEEEEECCCCCCCCCCEEEEHHHHHH
KYNVTNQKGIRRISTTAIDMLMRYHWPGNVRELENCIERAVILSEDNVIHGYHLPPSLQT
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCC
AESSGTPYTGSMMQKLDTIEKEMIIEALKRTKGNMSRAAAQLGLSDRIMGLRVKKFDIDY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHEEEEEEEECCE
RIFRP
EEECC
>Mature Secondary Structure
MLIPQQKELNSISLLAEVSRTVTNENDISKVLRIVLFIMSEHLDMLRGMITILNRETGEI
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
TTNESFGLTEEEMERGRYRIGEGIIGQVVKTGKPFIVPNIGDEPLFLDRTRSRQKTKKEN
ECCCCCCCCHHHHHCCHHHHCCHHHHHHHHCCCCEEECCCCCCCEEECCHHHHHHHHHCC
LCFICIPIRSGAEIIGTLSADRQLEPSLLKESGKRMKAEDERMDLLQHYVDLLSIIASMI
EEEEEEECCCCCCEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SQAVRLKQIAHEATAGIAVVEHKPQQEKKAGFMLGSKDAGEESIAESERPANIIGNSKPM
HHHHHHHHHHHHHHCCEEEEECCCCHHHHCCCEECCCCCCHHHHHHCCCCCHHCCCCCHH
LSLFRMIDKISKTNATTLVLGESGVGKELVASAIHFKSRREGNPFIKFNCAALPESIVES
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHH
ELFGHEKGSFTGASATRKGRFELAHTGTIFLDEVGELSLPIQAKLLRIIQEKEFERVGGS
HHHCCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
KTIKVDVRVIAATNRNLETLIREGLFREDLFYRLNIFPITVPPLRERKTDILLLADYFVE
EEEEEEEEEEEECCCCHHHHHHCCCHHHCCEEEEEEEEEECCCCCCCCCCEEEEHHHHHH
KYNVTNQKGIRRISTTAIDMLMRYHWPGNVRELENCIERAVILSEDNVIHGYHLPPSLQT
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCC
AESSGTPYTGSMMQKLDTIEKEMIIEALKRTKGNMSRAAAQLGLSDRIMGLRVKKFDIDY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHEEEEEEEECCE
RIFRP
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]