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The map label for this gene is purT

Identifier: 119357687

GI number: 119357687

Start: 2192007

End: 2193176

Strand: Reverse

Name: purT

Synonym: Cpha266_1891

Alternate gene names: 119357687

Gene position: 2193176-2192007 (Counterclockwise)

Preceding gene: 119357688

Following gene: 119357685

Centisome position: 69.98

GC content: 56.67

Gene sequence:

>1170_bases
ATGCAAAAAAAAATCATGCTGCTGGGCAGCGGGGAGCTGGGCAAGGAGTTTGTGATTGCCGTTAAACGTCTGGGACACTT
TGTGATTGCCGTTGACAGCTATAATGATGCTCCTGCGCAGCAGGTGGCTGACCGGCGTGAGGTGATCAATATGCTCGACG
GCGCGGCTCTCGATGCCATCGTTGCCCGGCATCAGCCCGATGTGATCGTGCCTGAAATCGAGGCTATTCGTACCGAGCGG
TTTTACGATTATGAAAAAGAGGGGATACAGGTTGTTCCTTCGGCCCGTGCCGCCAATTTCACCATGAACCGGAAAGCTAT
TCGCGATCTGGCTTCGAAAGAGCTTGGTCTTCGAACGGCGACGTATCGTTACGCAGCGTCGAAGGAAGAGCTGAAGAGGG
CGATAGGGGAGGTGGGAGTTCCCTGTGTGGTAAAACCGCTGATGAGCTCGTCGGGCAAGGGGCAGTCAACCGTTAAAACG
GAGGCTGACATTGAACATGCCTGGAGCTATTCGCAAAGCGGCAGGCGCGGTGATAGTGTGGAGGTGATTGTTGAGGCCTT
TGTGCCGTTCCATACCGAGATTACGCTCTTGACCGTCACGCAAAAAAACGGCCCGACGCTTTTCTGTCCGCCCATCGGGC
ACCGTCAGGAGCGGGGTGATTATCAGGAGAGCTGGCAGCCCTGCCGAATCGGCGATGCGCAGTTGCATGAAGCTCAGGAG
ATCGCTGAAAAAGTGACTCGTTCGCTGACAGGTGCGGGGATCTGGGGTGTGGAGTTTTTTCTGGCCGATGACGGGCTTTA
TTTTTCGGAGCTCTCCCCCCGTCCGCACGATACCGGCATGGTGACGCTGGCTGGTACGCAGAACTTTACGGAGTTCGAGC
TTCATGCGCGGGCGGTTTTAGGGCTTCCGATTCCGAAGATCGAACTGCTGCGGGTGGGTGCGAGCGCTGTGGTGTCAGCC
GACAGAGAGGGGAAGAACCCTGATTACAGCGGCCTTGAAGAGGCTCTCGGTGAGCCTTGCACCGATATTCGTATTTTCGG
AAAACCGGCAACCCGCCCTTATCGCCGAATGGGCGTAACGCTCGCTTACGACGAACCGGGCAGCGATGTCGATACGGTGA
AGGCGAAAGCCATTGCCAATGCCCGCAAGGTGAGGGTGACGAGCGAGTAG

Upstream 100 bases:

>100_bases
CCGTGCGCAAGCGGTGGGGCTTGTGTTTGCATAATGAGTTCCGATTGCCTGCCAATCAACCGCAGCAATCTCCTGACAGG
AAATTTAAAAAGCAATCAGA

Downstream 100 bases:

>100_bases
GTTTTGTCAGGAAAGGGAGAGGATTTCGTCTTCGAGAAGCTGATGGTTGTAGAGCTCCGCATAGAGGCTCTCTTGTGTAA
TGAGCTCTTCGTGCGTGCCG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MQKKIMLLGSGELGKEFVIAVKRLGHFVIAVDSYNDAPAQQVADRREVINMLDGAALDAIVARHQPDVIVPEIEAIRTER
FYDYEKEGIQVVPSARAANFTMNRKAIRDLASKELGLRTATYRYAASKEELKRAIGEVGVPCVVKPLMSSSGKGQSTVKT
EADIEHAWSYSQSGRRGDSVEVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRIGDAQLHEAQE
IAEKVTRSLTGAGIWGVEFFLADDGLYFSELSPRPHDTGMVTLAGTQNFTEFELHARAVLGLPIPKIELLRVGASAVVSA
DREGKNPDYSGLEEALGEPCTDIRIFGKPATRPYRRMGVTLAYDEPGSDVDTVKAKAIANARKVRVTSE

Sequences:

>Translated_389_residues
MQKKIMLLGSGELGKEFVIAVKRLGHFVIAVDSYNDAPAQQVADRREVINMLDGAALDAIVARHQPDVIVPEIEAIRTER
FYDYEKEGIQVVPSARAANFTMNRKAIRDLASKELGLRTATYRYAASKEELKRAIGEVGVPCVVKPLMSSSGKGQSTVKT
EADIEHAWSYSQSGRRGDSVEVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRIGDAQLHEAQE
IAEKVTRSLTGAGIWGVEFFLADDGLYFSELSPRPHDTGMVTLAGTQNFTEFELHARAVLGLPIPKIELLRVGASAVVSA
DREGKNPDYSGLEEALGEPCTDIRIFGKPATRPYRRMGVTLAYDEPGSDVDTVKAKAIANARKVRVTSE
>Mature_389_residues
MQKKIMLLGSGELGKEFVIAVKRLGHFVIAVDSYNDAPAQQVADRREVINMLDGAALDAIVARHQPDVIVPEIEAIRTER
FYDYEKEGIQVVPSARAANFTMNRKAIRDLASKELGLRTATYRYAASKEELKRAIGEVGVPCVVKPLMSSSGKGQSTVKT
EADIEHAWSYSQSGRRGDSVEVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRIGDAQLHEAQE
IAEKVTRSLTGAGIWGVEFFLADDGLYFSELSPRPHDTGMVTLAGTQNFTEFELHARAVLGLPIPKIELLRVGASAVVSA
DREGKNPDYSGLEEALGEPCTDIRIFGKPATRPYRRMGVTLAYDEPGSDVDTVKAKAIANARKVRVTSE

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=383, Percent_Identity=51.1749347258486, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786733, Length=361, Percent_Identity=25.4847645429363, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=25.4098360655738, Blast_Score=116, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_CHLPD (A1BHN0)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912331.1
- ProteinModelPortal:   A1BHN0
- SMR:   A1BHN0
- STRING:   A1BHN0
- GeneID:   4570850
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1891
- NMPDR:   fig|290317.7.peg.1995
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42771; Mature: 42771

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 72-72 BINDING 104-104 BINDING 145-145 BINDING 193-193 BINDING 258-258 BINDING 270-270 BINDING 277-277 BINDING 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKIMLLGSGELGKEFVIAVKRLGHFVIAVDSYNDAPAQQVADRREVINMLDGAALDAI
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
VARHQPDVIVPEIEAIRTERFYDYEKEGIQVVPSARAANFTMNRKAIRDLASKELGLRTA
HHCCCCCEECCCHHHHHHHHHCCCCCCCEEEECCCCCCCEEECHHHHHHHHHHHCCCCHH
TYRYAASKEELKRAIGEVGVPCVVKPLMSSSGKGQSTVKTEADIEHAWSYSQSGRRGDSV
HHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEECHHHHHHHHCCCCCCCCCCHH
EVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRIGDAQLHEAQE
HHHHHHHCCCCCEEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCCCCCHHHHHHHH
IAEKVTRSLTGAGIWGVEFFLADDGLYFSELSPRPHDTGMVTLAGTQNFTEFELHARAVL
HHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCEEEEECCCCCEEHHEEEEEEE
GLPIPKIELLRVGASAVVSADREGKNPDYSGLEEALGEPCTDIRIFGKPATRPYRRMGVT
CCCCCCCCEEECCHHHHEECCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCHHHHCCE
LAYDEPGSDVDTVKAKAIANARKVRVTSE
EEECCCCCCHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MQKKIMLLGSGELGKEFVIAVKRLGHFVIAVDSYNDAPAQQVADRREVINMLDGAALDAI
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
VARHQPDVIVPEIEAIRTERFYDYEKEGIQVVPSARAANFTMNRKAIRDLASKELGLRTA
HHCCCCCEECCCHHHHHHHHHCCCCCCCEEEECCCCCCCEEECHHHHHHHHHHHCCCCHH
TYRYAASKEELKRAIGEVGVPCVVKPLMSSSGKGQSTVKTEADIEHAWSYSQSGRRGDSV
HHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEECHHHHHHHHCCCCCCCCCCHH
EVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRIGDAQLHEAQE
HHHHHHHCCCCCEEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCCCCCHHHHHHHH
IAEKVTRSLTGAGIWGVEFFLADDGLYFSELSPRPHDTGMVTLAGTQNFTEFELHARAVL
HHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCEEEEECCCCCEEHHEEEEEEE
GLPIPKIELLRVGASAVVSADREGKNPDYSGLEEALGEPCTDIRIFGKPATRPYRRMGVT
CCCCCCCCEEECCHHHHEECCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCHHHHCCE
LAYDEPGSDVDTVKAKAIANARKVRVTSE
EEECCCCCCHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA