Click here to switch to the map view.

The map label for this gene is nadE2 [H]

Identifier: 119357679

GI number: 119357679

Start: 2179425

End: 2180279

Strand: Direct

Name: nadE2 [H]

Synonym: Cpha266_1883

Alternate gene names: 119357679

Gene position: 2179425-2180279 (Clockwise)

Preceding gene: 119357678

Following gene: 119357681

Centisome position: 69.54

GC content: 51.7

Gene sequence:

>855_bases
ATGTGCGCAAAACTGCGGATAGCCCAGACCGACTGCACCCTTGCCAATTTTGATGAAAATCTTGCACGGCACTGCGCCCT
GACTGAAGAGGCAATCCGGGATGGAGCGGATGCCATTGCCTTCCCTGAACTCTCACTCACGGGGTACAATGTTCAGGATG
CCGCACAGGACATGGCGATGCACCTGCACGACGAAAGGATGAAGCCTCTACGCGAACTCAGCAGCAGAATCTGCATCATC
TGCGGAGGAATTGAGCTCAGCGATGACTACGGCGTCTTCAACTCGGCATTCATGTTTGAGGATGGCCGGGCAGAAAGCGT
TCACCGTAAAATCTATCTCCCCACCTACGGCATGTTCGAAGAGCTCCGGTACTTTTCAGCAGGAAAGCATATTCAGGCTG
TAAACTCCAAAAGGTTGGGGAGAATCGGCATCGCAATCTGTGAAGATTTCTGGCACGTCTCCGTTCCCTACCTGCTTGCG
CATCAAGGCGCAAAGCTGCTGATCGTCATGATGTCAAGCCCTCTGAGGATGTCGCCGGGAAGCGGAACTCCCGAAATAGT
CAGCAAATGGCAGAATATTTCCTGTACCTATGCTTTTTTGTTCAGCAGTTACGTTGCCACCATCAACCGCACAGGCAATG
AAGACAGTTTCACCTACTGGGGCAACTCCTCCGTCACCGGGCCGGATGGAACAATCATTGCAGAAGCGCCGCTTTTTAAA
GAGATGGTCATGGACGCGAATATTGATTTTTCCGCTGTTAAACGGGCACGCCTTGAGTCCTCTCACTTTCTTGACGAGGA
TCTCAGACTTATTGCAGCCGAACTGGAAGCGAGTATCAAACGATCACGGTCATAA

Upstream 100 bases:

>100_bases
ACCGGCAGGGCAGGAGAGGTGCATCCGGGCATGTGGATCCTTGCACTGCTCTCATTTACCTTTTATCTTTTCTACCCATA
CCATTAAACAGGAGAAAAGG

Downstream 100 bases:

>100_bases
TCTTGACCCGGCCTGTGCTTCCATGGAAAATCCGGGAGGGGTAAGCGTAACCCGATACCCCTTTTGCTGCATGGCGCACA
CCGCGCCAGCTGCATTTTCC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MCAKLRIAQTDCTLANFDENLARHCALTEEAIRDGADAIAFPELSLTGYNVQDAAQDMAMHLHDERMKPLRELSSRICII
CGGIELSDDYGVFNSAFMFEDGRAESVHRKIYLPTYGMFEELRYFSAGKHIQAVNSKRLGRIGIAICEDFWHVSVPYLLA
HQGAKLLIVMMSSPLRMSPGSGTPEIVSKWQNISCTYAFLFSSYVATINRTGNEDSFTYWGNSSVTGPDGTIIAEAPLFK
EMVMDANIDFSAVKRARLESSHFLDEDLRLIAAELEASIKRSRS

Sequences:

>Translated_284_residues
MCAKLRIAQTDCTLANFDENLARHCALTEEAIRDGADAIAFPELSLTGYNVQDAAQDMAMHLHDERMKPLRELSSRICII
CGGIELSDDYGVFNSAFMFEDGRAESVHRKIYLPTYGMFEELRYFSAGKHIQAVNSKRLGRIGIAICEDFWHVSVPYLLA
HQGAKLLIVMMSSPLRMSPGSGTPEIVSKWQNISCTYAFLFSSYVATINRTGNEDSFTYWGNSSVTGPDGTIIAEAPLFK
EMVMDANIDFSAVKRARLESSHFLDEDLRLIAAELEASIKRSRS
>Mature_284_residues
MCAKLRIAQTDCTLANFDENLARHCALTEEAIRDGADAIAFPELSLTGYNVQDAAQDMAMHLHDERMKPLRELSSRICII
CGGIELSDDYGVFNSAFMFEDGRAESVHRKIYLPTYGMFEELRYFSAGKHIQAVNSKRLGRIGIAICEDFWHVSVPYLLA
HQGAKLLIVMMSSPLRMSPGSGTPEIVSKWQNISCTYAFLFSSYVATINRTGNEDSFTYWGNSSVTGPDGTIIAEAPLFK
EMVMDANIDFSAVKRARLESSHFLDEDLRLIAAELEASIKRSRS

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=24.7191011235955, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 31687; Mature: 31687

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAKLRIAQTDCTLANFDENLARHCALTEEAIRDGADAIAFPELSLTGYNVQDAAQDMAM
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEECCCCHHHHHHHHHH
HLHDERMKPLRELSSRICIICGGIELSDDYGVFNSAFMFEDGRAESVHRKIYLPTYGMFE
HHHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCEEEECCCCCCCCEEEEECCCHHHHH
ELRYFSAGKHIQAVNSKRLGRIGIAICEDFWHVSVPYLLAHQGAKLLIVMMSSPLRMSPG
HHHHHHCCCEEEHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCEECCC
SGTPEIVSKWQNISCTYAFLFSSYVATINRTGNEDSFTYWGNSSVTGPDGTIIAEAPLFK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEECCHHHH
EMVMDANIDFSAVKRARLESSHFLDEDLRLIAAELEASIKRSRS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCAKLRIAQTDCTLANFDENLARHCALTEEAIRDGADAIAFPELSLTGYNVQDAAQDMAM
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEECCCCHHHHHHHHHH
HLHDERMKPLRELSSRICIICGGIELSDDYGVFNSAFMFEDGRAESVHRKIYLPTYGMFE
HHHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCEEEECCCCCCCCEEEEECCCHHHHH
ELRYFSAGKHIQAVNSKRLGRIGIAICEDFWHVSVPYLLAHQGAKLLIVMMSSPLRMSPG
HHHHHHCCCEEEHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCEECCC
SGTPEIVSKWQNISCTYAFLFSSYVATINRTGNEDSFTYWGNSSVTGPDGTIIAEAPLFK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEECCHHHH
EMVMDANIDFSAVKRARLESSHFLDEDLRLIAAELEASIKRSRS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]