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The map label for this gene is metK

Identifier: 119356750

GI number: 119356750

Start: 1050001

End: 1051215

Strand: Reverse

Name: metK

Synonym: Cpha266_0922

Alternate gene names: 119356750

Gene position: 1051215-1050001 (Counterclockwise)

Preceding gene: 119356751

Following gene: 119356749

Centisome position: 33.54

GC content: 51.6

Gene sequence:

>1215_bases
ATGTCACAATCAAGGTATTTTTTTACTTCAGAATCCGTATCGGAAGGGCATCCAGACAAAGTATCCGATCAGATTTCCGA
TGCTGTACTTGATGAGTTTATTCGCCAGGATCCGGATTCACGCGTTGCCTGCGAAACGTTCGTCACCACCGGTCAGGTTA
TTGTAGGCGGAGAAGTAACGACCAAGGGTGTTGTTGACGTTCAGACAATCGCTCGTAAAGTCATCACCGAAATAGGCTAT
ACCAAAGGCGAATACATGTTTGACGCTCATTCATGCGGCGTTCTCTCTGCACTTCACAGCCAGTCGCCGGATATCAACCG
TGGCGTTGACCGCAAGGAAGAGATCTCTGACGAACTTGACCGCGTCGGAGCAGGTGACCAGGGTATGATGTTCGGCTATG
CATGCACCGAAACGCCTGAACTGATGCCTGCCGCTCTCCAGTTCGCCCAGCAACTTGTCAAGAAGCTTGCTGAAATCCGC
AAGGAAGGAAAAATCATGACCTACCTTCGCCCCGACGCCAAAAGCCAGGTTACGCTTGAGTATGTGGACGAACAGGTCAA
ACGAGTTGATGCCGTTGTGGTATCAACACAACACGACCCTGAACCTGAAGGCGTCAGCGAAGCCGAGTGGCAGGCAGTCA
TCAAAAAAGATATTATCGAGAATGTCATCAACGTCGTTATTCCTGCGGCTCTGCTCGATGAAAAAACAAAACTGCACATC
AACCCGACCGGACGTTTTGTCATCGGCGGACCACACGGCGATACCGGCCTGACCGGACGCAAGATCATTGTGGATACCTA
TGGCGGCGCAGCGCCCCACGGCGGCGGCGCATTCAGCGGTAAAGATCCGTCGAAAGTCGACCGCAGCGCGGCATATGCTT
CGCGTCACGTCGCAAAGAACATTGTTGCAGCGGGACTTGCCGATAAATGTACCGTTCAGGTCTCCTATGCAATCGGCGTT
GCCCAGCCCGTTTCGATCTATATCAATACCCACGGCACAGCAAAACATGGACTCACCGATGCAGAAATTCAGGAAAAGGC
CGAAGTTATCTTCGATCTTCGTCCTGCATCGATCATCAAGCGTTTCGGCCTCAACAAACCTGAAGGCTGGTGCTACCAGG
AAACCGCAGCCTATGGCCATTTCGGCCGTGAAAACTTCCCGTGGGAAAGAACCGAAAAAGTTGAAGAGCTGAAAAAAGCC
CTAAATGTAGCCTAA

Upstream 100 bases:

>100_bases
TTTCATGCATCATCCTCCAGCGAAAAATCTTTCTTCTTAACCCCAGGTTTTTTTTCCTGTTGATTCTTGTTGTTCGTTTA
ACCAAAACTTGTTGAAAAAA

Downstream 100 bases:

>100_bases
AAACAATCAGAGAACCTCTGTTTATTACCGGCAAGCGACTGGCGGAGTACCGACCCGTCTGAAAATCGAGCATGTAATAA
AGAGAGGTTCCCGTTCCTCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSQSRYFFTSESVSEGHPDKVSDQISDAVLDEFIRQDPDSRVACETFVTTGQVIVGGEVTTKGVVDVQTIARKVITEIGY
TKGEYMFDAHSCGVLSALHSQSPDINRGVDRKEEISDELDRVGAGDQGMMFGYACTETPELMPAALQFAQQLVKKLAEIR
KEGKIMTYLRPDAKSQVTLEYVDEQVKRVDAVVVSTQHDPEPEGVSEAEWQAVIKKDIIENVINVVIPAALLDEKTKLHI
NPTGRFVIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVAKNIVAAGLADKCTVQVSYAIGV
AQPVSIYINTHGTAKHGLTDAEIQEKAEVIFDLRPASIIKRFGLNKPEGWCYQETAAYGHFGRENFPWERTEKVEELKKA
LNVA

Sequences:

>Translated_404_residues
MSQSRYFFTSESVSEGHPDKVSDQISDAVLDEFIRQDPDSRVACETFVTTGQVIVGGEVTTKGVVDVQTIARKVITEIGY
TKGEYMFDAHSCGVLSALHSQSPDINRGVDRKEEISDELDRVGAGDQGMMFGYACTETPELMPAALQFAQQLVKKLAEIR
KEGKIMTYLRPDAKSQVTLEYVDEQVKRVDAVVVSTQHDPEPEGVSEAEWQAVIKKDIIENVINVVIPAALLDEKTKLHI
NPTGRFVIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVAKNIVAAGLADKCTVQVSYAIGV
AQPVSIYINTHGTAKHGLTDAEIQEKAEVIFDLRPASIIKRFGLNKPEGWCYQETAAYGHFGRENFPWERTEKVEELKKA
LNVA
>Mature_403_residues
SQSRYFFTSESVSEGHPDKVSDQISDAVLDEFIRQDPDSRVACETFVTTGQVIVGGEVTTKGVVDVQTIARKVITEIGYT
KGEYMFDAHSCGVLSALHSQSPDINRGVDRKEEISDELDRVGAGDQGMMFGYACTETPELMPAALQFAQQLVKKLAEIRK
EGKIMTYLRPDAKSQVTLEYVDEQVKRVDAVVVSTQHDPEPEGVSEAEWQAVIKKDIIENVINVVIPAALLDEKTKLHIN
PTGRFVIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVAKNIVAAGLADKCTVQVSYAIGVA
QPVSIYINTHGTAKHGLTDAEIQEKAEVIFDLRPASIIKRFGLNKPEGWCYQETAAYGHFGRENFPWERTEKVEELKKAL
NVA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=401, Percent_Identity=51.8703241895262, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI4557737, Length=400, Percent_Identity=51.25, Blast_Score=371, Evalue=1e-103,
Organism=Escherichia coli, GI1789311, Length=397, Percent_Identity=58.4382871536524, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17538494, Length=407, Percent_Identity=53.0712530712531, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538498, Length=399, Percent_Identity=52.6315789473684, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25145633, Length=399, Percent_Identity=52.8822055137845, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17551082, Length=396, Percent_Identity=51.7676767676768, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32565905, Length=407, Percent_Identity=48.4029484029484, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32565911, Length=348, Percent_Identity=50.5747126436782, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25145635, Length=348, Percent_Identity=50.8620689655172, Blast_Score=313, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI32565903, Length=329, Percent_Identity=51.063829787234, Blast_Score=284, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI32565909, Length=80, Percent_Identity=61.25, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=64, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=400, Percent_Identity=51.75, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323209, Length=394, Percent_Identity=50.761421319797, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45552159, Length=396, Percent_Identity=50.7575757575758, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580529, Length=396, Percent_Identity=50.7575757575758, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580537, Length=396, Percent_Identity=50.7575757575758, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580533, Length=396, Percent_Identity=50.7575757575758, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580535, Length=396, Percent_Identity=50.7575757575758, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580531, Length=396, Percent_Identity=50.7575757575758, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580527, Length=394, Percent_Identity=50.253807106599, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24580525, Length=394, Percent_Identity=50.253807106599, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24580541, Length=381, Percent_Identity=49.3438320209974, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=49.1803278688525, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CHLPD (A1BEZ3)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911394.1
- ProteinModelPortal:   A1BEZ3
- SMR:   A1BEZ3
- STRING:   A1BEZ3
- GeneID:   4570613
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0922
- NMPDR:   fig|290317.7.peg.979
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44081; Mature: 43950

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSRYFFTSESVSEGHPDKVSDQISDAVLDEFIRQDPDSRVACETFVTTGQVIVGGEVT
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCEEECCCCC
TKGVVDVQTIARKVITEIGYTKGEYMFDAHSCGVLSALHSQSPDINRGVDRKEEISDELD
CCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RVGAGDQGMMFGYACTETPELMPAALQFAQQLVKKLAEIRKEGKIMTYLRPDAKSQVTLE
HCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHH
YVDEQVKRVDAVVVSTQHDPEPEGVSEAEWQAVIKKDIIENVINVVIPAALLDEKTKLHI
HHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NPTGRFVIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IVAAGLADKCTVQVSYAIGVAQPVSIYINTHGTAKHGLTDAEIQEKAEVIFDLRPASIIK
HHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCHHCCCCHHHHHHHHHHHHCCCHHHHHH
RFGLNKPEGWCYQETAAYGHFGRENFPWERTEKVEELKKALNVA
HHCCCCCCCCEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQSRYFFTSESVSEGHPDKVSDQISDAVLDEFIRQDPDSRVACETFVTTGQVIVGGEVT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCEEECCCCC
TKGVVDVQTIARKVITEIGYTKGEYMFDAHSCGVLSALHSQSPDINRGVDRKEEISDELD
CCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RVGAGDQGMMFGYACTETPELMPAALQFAQQLVKKLAEIRKEGKIMTYLRPDAKSQVTLE
HCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHH
YVDEQVKRVDAVVVSTQHDPEPEGVSEAEWQAVIKKDIIENVINVVIPAALLDEKTKLHI
HHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NPTGRFVIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IVAAGLADKCTVQVSYAIGVAQPVSIYINTHGTAKHGLTDAEIQEKAEVIFDLRPASIIK
HHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCHHCCCCHHHHHHHHHHHHCCCHHHHHH
RFGLNKPEGWCYQETAAYGHFGRENFPWERTEKVEELKKALNVA
HHCCCCCCCCEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA