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The map label for this gene is nifK [H]

Identifier: 119356572

GI number: 119356572

Start: 838633

End: 839985

Strand: Reverse

Name: nifK [H]

Synonym: Cpha266_0738

Alternate gene names: 119356572

Gene position: 839985-838633 (Counterclockwise)

Preceding gene: 119356573

Following gene: 119356571

Centisome position: 26.8

GC content: 51.0

Gene sequence:

>1353_bases
ATGAAACATGCAAAAACAGCAACACAAAACGCCTGCAAACTGTGCAACCCGCTCGGGGCATGCCTTGCTTTCAGGGGAAT
CGAAAACTGCGTACCCTTCCTGCACGGTTCACAAGGTTGCGCAACCTATATCCGTCGTTACCTGATAAGCCATTACAAGG
AACCGATCGATATCGCTTCGTCGAACTTCAATGAAGAAACCGCCGTGTTTGGAGGCAGTCATAACCTGCAGCTTGGACTG
AAAAACGTCACCGAGCAGTACAAGCCTGAGGTTATCGGACTGGCCACAACATGCCTGAGCGAAACCATCGGGGATGATGT
GCCGATGATCCTTCGCGACTATAAAAAAGCGTTTAAAAACGGTACGCCAATGCCGATAATGATTCATGCCTCAACGCCAA
GCTATCAGGGAAGCCACATCGACGGCTTTCATGCCGCTGTCAGGGCAAGCGTTAAAACCCTTGCTGAAAAAGGGGCACGA
AAAAACCTGATCAATATCTTCCCGAACATGATCTCGCCGGCGGATATTCGTTACATCAAGGAGATTCTCTCCGATTTCAG
CGTGCCCTATATGCTCCTGCCAGACTACTCACAGACGATGGACGGCGGGCCATGGGGCGAATACCACCGCATCCCGCCAG
GAGGGACACCTGCCGGAGCCATTGCCGGTGCAGGATGCGCAACGGCAAGTATCGAGTTCGGCTCTACCCTTGAATCCTCA
AAATCTGCCGCCGGCTACCTTGAGGAGACATTCGACGTTCCCCGTTACCCTCTTGCCCTGCCGATCGGAATAAACGAGAG
CGACAGACTGTTCAACCTGCTTGAAAAACTGACCGAACAGAAAATGCCGGAAAAATATGAGGATGAACGACGCCGTCTGG
TTGACGCTTATGCCGATGGGCACAAATATGTTTTTGAGAAAAAGGTCATTCTGTACGGAGAGGAAGACCTGGTGATCTCC
ATGGCAGCGTTTCTGCGTGAAATCGGCATGACCCCCGTACTCTGCGCGTCGGGAGGCAAAAGCGGTCTGATGAGAAAAAA
GCTTCTTGAACTGATTCCCGACCTTGAGGAACAGGGCATCAGGATACGTGACGGAGTGGACTTTGTCGATATTGAAGACG
AAGCCAAAGTACTGCTCCCTGACCTCCTGATCGGCAATAGTAAAGGCTTTACGATGGCACGAAAAAACAACATTCCGCTG
ATGAGAATCGGCTTTCCGATTCATGACCGGTTCGGTGGACAGCGAATGCATCATATCGGGTATCGCGGTACCCAGGAACT
CTTTGACCGGATAGTCAACACCGTTATCGAACAACGGCAGAATGCTTCATCAATAGGCTATACCTACATGTAA

Upstream 100 bases:

>100_bases
CTCTCGCCGGATTTATCGGTATGTACAATTTTGCACTGGAGGTCTATCAATCGGTCATGAGTCCGGTATGGCAGTTCGCT
CCGAGAAAAGGAGGTAACGC

Downstream 100 bases:

>100_bases
GGGAGGATATTATGACACTGAAACTTGAAAACCATCCCTGCTTTAACGACTCAGCGCGACACACTTTCGGACGCATCCAC
CTCCCCATTGCACCGAAATG

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKHAKTATQNACKLCNPLGACLAFRGIENCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSHNLQLGL
KNVTEQYKPEVIGLATTCLSETIGDDVPMILRDYKKAFKNGTPMPIMIHASTPSYQGSHIDGFHAAVRASVKTLAEKGAR
KNLINIFPNMISPADIRYIKEILSDFSVPYMLLPDYSQTMDGGPWGEYHRIPPGGTPAGAIAGAGCATASIEFGSTLESS
KSAAGYLEETFDVPRYPLALPIGINESDRLFNLLEKLTEQKMPEKYEDERRRLVDAYADGHKYVFEKKVILYGEEDLVIS
MAAFLREIGMTPVLCASGGKSGLMRKKLLELIPDLEEQGIRIRDGVDFVDIEDEAKVLLPDLLIGNSKGFTMARKNNIPL
MRIGFPIHDRFGGQRMHHIGYRGTQELFDRIVNTVIEQRQNASSIGYTYM

Sequences:

>Translated_450_residues
MKHAKTATQNACKLCNPLGACLAFRGIENCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSHNLQLGL
KNVTEQYKPEVIGLATTCLSETIGDDVPMILRDYKKAFKNGTPMPIMIHASTPSYQGSHIDGFHAAVRASVKTLAEKGAR
KNLINIFPNMISPADIRYIKEILSDFSVPYMLLPDYSQTMDGGPWGEYHRIPPGGTPAGAIAGAGCATASIEFGSTLESS
KSAAGYLEETFDVPRYPLALPIGINESDRLFNLLEKLTEQKMPEKYEDERRRLVDAYADGHKYVFEKKVILYGEEDLVIS
MAAFLREIGMTPVLCASGGKSGLMRKKLLELIPDLEEQGIRIRDGVDFVDIEDEAKVLLPDLLIGNSKGFTMARKNNIPL
MRIGFPIHDRFGGQRMHHIGYRGTQELFDRIVNTVIEQRQNASSIGYTYM
>Mature_450_residues
MKHAKTATQNACKLCNPLGACLAFRGIENCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSHNLQLGL
KNVTEQYKPEVIGLATTCLSETIGDDVPMILRDYKKAFKNGTPMPIMIHASTPSYQGSHIDGFHAAVRASVKTLAEKGAR
KNLINIFPNMISPADIRYIKEILSDFSVPYMLLPDYSQTMDGGPWGEYHRIPPGGTPAGAIAGAGCATASIEFGSTLESS
KSAAGYLEETFDVPRYPLALPIGINESDRLFNLLEKLTEQKMPEKYEDERRRLVDAYADGHKYVFEKKVILYGEEDLVIS
MAAFLREIGMTPVLCASGGKSGLMRKKLLELIPDLEEQGIRIRDGVDFVDIEDEAKVLLPDLLIGNSKGFTMARKNNIPL
MRIGFPIHDRFGGQRMHHIGYRGTQELFDRIVNTVIEQRQNASSIGYTYM

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 49927; Mature: 49927

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAKTATQNACKLCNPLGACLAFRGIENCVPFLHGSQGCATYIRRYLISHYKEPIDIAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHC
SNFNEETAVFGGSHNLQLGLKNVTEQYKPEVIGLATTCLSETIGDDVPMILRDYKKAFKN
CCCCCCCEEECCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GTPMPIMIHASTPSYQGSHIDGFHAAVRASVKTLAEKGARKNLINIFPNMISPADIRYIK
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
EILSDFSVPYMLLPDYSQTMDGGPWGEYHRIPPGGTPAGAIAGAGCATASIEFGSTLESS
HHHHHCCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCHHHC
KSAAGYLEETFDVPRYPLALPIGINESDRLFNLLEKLTEQKMPEKYEDERRRLVDAYADG
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
HKYVFEKKVILYGEEDLVISMAAFLREIGMTPVLCASGGKSGLMRKKLLELIPDLEEQGI
CEEEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCHHHCCE
RIRDGVDFVDIEDEAKVLLPDLLIGNSKGFTMARKNNIPLMRIGFPIHDRFGGQRMHHIG
EEECCCCEEECCCCCHHEECHHEECCCCCCEEEECCCCCEEEECCCHHHCCCCCHHHHCC
YRGTQELFDRIVNTVIEQRQNASSIGYTYM
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKHAKTATQNACKLCNPLGACLAFRGIENCVPFLHGSQGCATYIRRYLISHYKEPIDIAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHC
SNFNEETAVFGGSHNLQLGLKNVTEQYKPEVIGLATTCLSETIGDDVPMILRDYKKAFKN
CCCCCCCEEECCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GTPMPIMIHASTPSYQGSHIDGFHAAVRASVKTLAEKGARKNLINIFPNMISPADIRYIK
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
EILSDFSVPYMLLPDYSQTMDGGPWGEYHRIPPGGTPAGAIAGAGCATASIEFGSTLESS
HHHHHCCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCHHHC
KSAAGYLEETFDVPRYPLALPIGINESDRLFNLLEKLTEQKMPEKYEDERRRLVDAYADG
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
HKYVFEKKVILYGEEDLVISMAAFLREIGMTPVLCASGGKSGLMRKKLLELIPDLEEQGI
CEEEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCHHHCCE
RIRDGVDFVDIEDEAKVLLPDLLIGNSKGFTMARKNNIPLMRIGFPIHDRFGGQRMHHIG
EEECCCCEEECCCCCHHEECHHEECCCCCCEEEECCCCCEEEECCCHHHCCCCCHHHHCC
YRGTQELFDRIVNTVIEQRQNASSIGYTYM
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840948; 3473447; 8393705 [H]