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The map label for this gene is nifK [H]

Identifier: 119356573

GI number: 119356573

Start: 841361

End: 842740

Strand: Reverse

Name: nifK [H]

Synonym: Cpha266_0740

Alternate gene names: 119356573

Gene position: 842740-841361 (Counterclockwise)

Preceding gene: 119356574

Following gene: 119356572

Centisome position: 26.89

GC content: 51.74

Gene sequence:

>1380_bases
ATGTTATTAAGACACACGACAAAAGAGGTTAAAGAGCGTGAGGGGCTGACGATCAATCCGGCAAAAACCTGCCAGCCGAT
CGGAGCCATGTACGCCGCGCTCGGTATTCATGGCTGTCTGCCTCACAGCCATGGCTCACAAGGGTGCTGCTCCTATCATC
GCAGCACCCTGACCCGCCACTACAAGGAACCAGTGATGGCGGCAACCAGTTCTTTCACAGAAGGCGCTTCGGTGTTCGGA
GGTCAGGCAAACCTGCTGGCTGCCATCGATACGATCTTTTCGGTATATAACCCGGATATCATTGCGGTTCACTCGACCTG
CCTTAGCGAAACCATCGGTGACGATTTGCAGCAGATCACTAAAAAAGCAAAGGACGATGGCAAAATTCCGGAGGGCAAAC
ACATCATTTACGCCAGCACGCCAAGCTTTGTAGGATCGCATGTAACGGGTTATGCCAACATGGTTGCGGGCATGGCCGAT
CAGTTTGCCGTTTCAACCGACGAAAAAAAGAACCAGATCAACATCATTGCCGGATGGATGGAACCGTCGGACATGCGCGA
AATCAAAAGGCTCTCCACCGAACTCGGCGTCAAAATCGTGCTCTTTCCTGATACCTCAAACGTGCTCGATGCGCCGCAGA
CAGGCAAACATGAGTTCTATCCGAAAGGCGGCACGACGGTCGAGGAGCTCAAAAGCACCGGCGACAGCATTCGATCGCTT
GCGCTCGGATGCATCAGCGCGGAACCGGCGGCAATCGCTCTTGAAAAAAAGTGCAAGGTTCCGTTTGAAACTCTCGATAT
GCCAATTGGCATTTCAGCAACCGATCGCTTCATCATGGCGCTGAGCCAGGCGGCAGGCGTTACGGTTCCTGATGACATAA
CAGCCGACAGAGGCCGACTGGTAGATATCATTGCCGACATGGAACAGTATTTATTCGGCAAAAAAGTTGCCTTATTCGGT
GATCCCGACCAGCTTATTCCGCTCACCGAGTTTCTGCTGGATCTCAACATGAAACCGGTTCATATCGTCAGCGGGACACC
TGGACAGCGTTTTGAGAAACGAATGAAGGAGATCTTGCAGAGAGCTCCCGGTGCAAACTTCAAAAACGGTCTCGGCGCGG
ATATGTTCCTGCTTCACCAGTGGATTAAAAATGAGCCTGTTGATCTCCTGATCGGCAACACCTACGGCAAGTACATTGCC
CGTGACGAAGACATCCCGTTTATCAGGTATGGCTTCCCGATTCTCGACCGTATTGGACACAGCTATTTTCCGAGCGTCGG
GTACACAGGAGGAATGAGACTTGTCGAGAAAATTCTCGGGGCACTTATGGATCGTCAGGATCGCGATGCGCTTGAAGAAA
AATTTGAGCTCGTGATGTAA

Upstream 100 bases:

>100_bases
CTCATCAAGGCCCCGTGGCAGAAAGCGGAAAACGGATCATCGACAGCATTGGAAGCGAGTTACGTCACTCACTAAACAGG
AAAACGGAGACTATTGCATT

Downstream 100 bases:

>100_bases
AAAAAAGAGTCGTACAACATTTCATTACAGAGGTGAAGCCATGGAACAGATTGGTATACTTGAAGGCAGAGAGAAACAGA
TTTTCGAAAAGAAAAGCGGA

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLAAIDTIFSVYNPDIIAVHSTCLSETIGDDLQQITKKAKDDGKIPEGKHIIYASTPSFVGSHVTGYANMVAGMAD
QFAVSTDEKKNQINIIAGWMEPSDMREIKRLSTELGVKIVLFPDTSNVLDAPQTGKHEFYPKGGTTVEELKSTGDSIRSL
ALGCISAEPAAIALEKKCKVPFETLDMPIGISATDRFIMALSQAAGVTVPDDITADRGRLVDIIADMEQYLFGKKVALFG
DPDQLIPLTEFLLDLNMKPVHIVSGTPGQRFEKRMKEILQRAPGANFKNGLGADMFLLHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFIRYGFPILDRIGHSYFPSVGYTGGMRLVEKILGALMDRQDRDALEEKFELVM

Sequences:

>Translated_459_residues
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLAAIDTIFSVYNPDIIAVHSTCLSETIGDDLQQITKKAKDDGKIPEGKHIIYASTPSFVGSHVTGYANMVAGMAD
QFAVSTDEKKNQINIIAGWMEPSDMREIKRLSTELGVKIVLFPDTSNVLDAPQTGKHEFYPKGGTTVEELKSTGDSIRSL
ALGCISAEPAAIALEKKCKVPFETLDMPIGISATDRFIMALSQAAGVTVPDDITADRGRLVDIIADMEQYLFGKKVALFG
DPDQLIPLTEFLLDLNMKPVHIVSGTPGQRFEKRMKEILQRAPGANFKNGLGADMFLLHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFIRYGFPILDRIGHSYFPSVGYTGGMRLVEKILGALMDRQDRDALEEKFELVM
>Mature_459_residues
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLAAIDTIFSVYNPDIIAVHSTCLSETIGDDLQQITKKAKDDGKIPEGKHIIYASTPSFVGSHVTGYANMVAGMAD
QFAVSTDEKKNQINIIAGWMEPSDMREIKRLSTELGVKIVLFPDTSNVLDAPQTGKHEFYPKGGTTVEELKSTGDSIRSL
ALGCISAEPAAIALEKKCKVPFETLDMPIGISATDRFIMALSQAAGVTVPDDITADRGRLVDIIADMEQYLFGKKVALFG
DPDQLIPLTEFLLDLNMKPVHIVSGTPGQRFEKRMKEILQRAPGANFKNGLGADMFLLHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFIRYGFPILDRIGHSYFPSVGYTGGMRLVEKILGALMDRQDRDALEEKFELVM

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50359; Mature: 50359

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRH
CCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
YKEPVMAATSSFTEGASVFGGQANLLAAIDTIFSVYNPDIIAVHSTCLSETIGDDLQQIT
HHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
KKAKDDGKIPEGKHIIYASTPSFVGSHVTGYANMVAGMADQFAVSTDEKKNQINIIAGWM
HHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECC
EPSDMREIKRLSTELGVKIVLFPDTSNVLDAPQTGKHEFYPKGGTTVEELKSTGDSIRSL
CCHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ALGCISAEPAAIALEKKCKVPFETLDMPIGISATDRFIMALSQAAGVTVPDDITADRGRL
HHHHCCCCCCCEEEHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHH
VDIIADMEQYLFGKKVALFGDPDQLIPLTEFLLDLNMKPVHIVSGTPGQRFEKRMKEILQ
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
RAPGANFKNGLGADMFLLHQWIKNEPVDLLIGNTYGKYIARDEDIPFIRYGFPILDRIGH
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCEEEECCHHHHHCCH
SYFPSVGYTGGMRLVEKILGALMDRQDRDALEEKFELVM
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRH
CCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
YKEPVMAATSSFTEGASVFGGQANLLAAIDTIFSVYNPDIIAVHSTCLSETIGDDLQQIT
HHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
KKAKDDGKIPEGKHIIYASTPSFVGSHVTGYANMVAGMADQFAVSTDEKKNQINIIAGWM
HHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECC
EPSDMREIKRLSTELGVKIVLFPDTSNVLDAPQTGKHEFYPKGGTTVEELKSTGDSIRSL
CCHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ALGCISAEPAAIALEKKCKVPFETLDMPIGISATDRFIMALSQAAGVTVPDDITADRGRL
HHHHCCCCCCCEEEHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHH
VDIIADMEQYLFGKKVALFGDPDQLIPLTEFLLDLNMKPVHIVSGTPGQRFEKRMKEILQ
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
RAPGANFKNGLGADMFLLHQWIKNEPVDLLIGNTYGKYIARDEDIPFIRYGFPILDRIGH
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCEEEECCHHHHHCCH
SYFPSVGYTGGMRLVEKILGALMDRQDRDALEEKFELVM
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840948; 3473447; 8393705 [H]