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The map label for this gene is nifB [H]

Identifier: 119356571

GI number: 119356571

Start: 837350

End: 838621

Strand: Reverse

Name: nifB [H]

Synonym: Cpha266_0737

Alternate gene names: 119356571

Gene position: 838621-837350 (Counterclockwise)

Preceding gene: 119356572

Following gene: 119356570

Centisome position: 26.76

GC content: 51.81

Gene sequence:

>1272_bases
ATGACACTGAAACTTGAAAACCATCCCTGCTTTAACGACTCAGCGCGACACACTTTCGGACGCATCCACCTCCCCATTGC
ACCGAAATGCAATATTCAGTGCAATTACTGCAACAGGAAGTTTGACTGCATGAACGAAAACAGGCCCGGAGTCACCAGCA
AGATTCTCTCGCCACTGCAGGCGATGCACTATCTTGAACAGGCCATCGCTCTGACCCCCTCTATCGCTGTAGTGGGAATC
GCGGGGCCGGGAGATCCATTTGCAAATCCCGACGAGACGATGGAGACGCTCAGGCTGGTGAGAGCAAAGTATCCTGAAAT
GCTGCTTTGCGTAGCCACAAACGGACTTGATGTTCTTCCGTACATCAACGAACTTGCTGAACTGAAGGTAAGCCATGTAA
CCATTACCATCAATGCCATCGACCCTGAAATCGGATCGGAAATCTATGCATGGGTACGATATAACAAGAAAATGTACCGC
GATGTTGAAGCAGCGGAACTGTTGATCAATAAACAGCTTGAGGCGTTGAAAAAGCTCAAGGAAGCGGGCGTTACCGCCAA
AGTGAACTCGATTATCATACCGGGCATCAACGATACGCATGTTATCGAGGTCGCACGAAAAGTCGCCGGGCTTGGTGCAG
ATATTCTGAACTGCATGCCCTATTACAGCACCACCGAAACGGTATTTGAAAACATTGACGAGCCATCTCCGGAGGTAGTC
AGCGAAATACAGAACGCAACGGCGGAATTCCTGCCGCAAATGAAACACTGTGCCCGCTGCCGTGCCGATGCTGTAGGGAT
TATCGGGGAAATCAACAGTGACGAGATGATTCAAAAACTCGCCGAGGCAGCAAGCATGCCGAAAAATCCCGGTGAGCATC
GTCCCTTTATAGCGGTATCGAGCCTGGAGGGAGTGCTCGTCAATCAGCATCTTGGTGAAGCCGATCGGTTTCTCATCTAT
GGTCAGGACAAGAATGGCTGCTCCGTACTCCTCGATTCGCGGGCAGCGCCTCCTTCCGGGGGCGGTCAGGAAAGATGGAA
GGCTCTTGCCGATCTGCTGAAAGACTGCCGTGCACTCCTGGTGAATGGTGCCGGAGATTCACCCACAAAAGTGTTGAACG
CAAACGGGATTGAAGTCATGGTTCTTGAAGGGATGATCGAAGATGCGGTAAGCGGCGTGTTTAACGGCCTGAGCCTGAAA
CATCTGATGAAAAGCAGCCAGATCCATGCCTGCGGAACAAGCTGTACCGGCACGGGAGGCGGGTGCGGTTAA

Upstream 100 bases:

>100_bases
GCGGTACCCAGGAACTCTTTGACCGGATAGTCAACACCGTTATCGAACAACGGCAGAATGCTTCATCAATAGGCTATACC
TACATGTAAGGGAGGATATT

Downstream 100 bases:

>100_bases
AAGAGCTGCTGAATAGTGAGGTTTCCCGGTATCAACCCGAACAATGAGCGCGATCGGGCCTTCTGACATTAATTATGCAC
CGGAAAAAAAGAGGTTGAGC

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTLKLENHPCFNDSARHTFGRIHLPIAPKCNIQCNYCNRKFDCMNENRPGVTSKILSPLQAMHYLEQAIALTPSIAVVGI
AGPGDPFANPDETMETLRLVRAKYPEMLLCVATNGLDVLPYINELAELKVSHVTITINAIDPEIGSEIYAWVRYNKKMYR
DVEAAELLINKQLEALKKLKEAGVTAKVNSIIIPGINDTHVIEVARKVAGLGADILNCMPYYSTTETVFENIDEPSPEVV
SEIQNATAEFLPQMKHCARCRADAVGIIGEINSDEMIQKLAEAASMPKNPGEHRPFIAVSSLEGVLVNQHLGEADRFLIY
GQDKNGCSVLLDSRAAPPSGGGQERWKALADLLKDCRALLVNGAGDSPTKVLNANGIEVMVLEGMIEDAVSGVFNGLSLK
HLMKSSQIHACGTSCTGTGGGCG

Sequences:

>Translated_423_residues
MTLKLENHPCFNDSARHTFGRIHLPIAPKCNIQCNYCNRKFDCMNENRPGVTSKILSPLQAMHYLEQAIALTPSIAVVGI
AGPGDPFANPDETMETLRLVRAKYPEMLLCVATNGLDVLPYINELAELKVSHVTITINAIDPEIGSEIYAWVRYNKKMYR
DVEAAELLINKQLEALKKLKEAGVTAKVNSIIIPGINDTHVIEVARKVAGLGADILNCMPYYSTTETVFENIDEPSPEVV
SEIQNATAEFLPQMKHCARCRADAVGIIGEINSDEMIQKLAEAASMPKNPGEHRPFIAVSSLEGVLVNQHLGEADRFLIY
GQDKNGCSVLLDSRAAPPSGGGQERWKALADLLKDCRALLVNGAGDSPTKVLNANGIEVMVLEGMIEDAVSGVFNGLSLK
HLMKSSQIHACGTSCTGTGGGCG
>Mature_422_residues
TLKLENHPCFNDSARHTFGRIHLPIAPKCNIQCNYCNRKFDCMNENRPGVTSKILSPLQAMHYLEQAIALTPSIAVVGIA
GPGDPFANPDETMETLRLVRAKYPEMLLCVATNGLDVLPYINELAELKVSHVTITINAIDPEIGSEIYAWVRYNKKMYRD
VEAAELLINKQLEALKKLKEAGVTAKVNSIIIPGINDTHVIEVARKVAGLGADILNCMPYYSTTETVFENIDEPSPEVVS
EIQNATAEFLPQMKHCARCRADAVGIIGEINSDEMIQKLAEAASMPKNPGEHRPFIAVSSLEGVLVNQHLGEADRFLIYG
QDKNGCSVLLDSRAAPPSGGGQERWKALADLLKDCRALLVNGAGDSPTKVLNANGIEVMVLEGMIEDAVSGVFNGLSLKH
LMKSSQIHACGTSCTGTGGGCG

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 45810; Mature: 45679

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKLENHPCFNDSARHTFGRIHLPIAPKCNIQCNYCNRKFDCMNENRPGVTSKILSPLQ
CEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHH
AMHYLEQAIALTPSIAVVGIAGPGDPFANPDETMETLRLVRAKYPEMLLCVATNGLDVLP
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
YINELAELKVSHVTITINAIDPEIGSEIYAWVRYNKKMYRDVEAAELLINKQLEALKKLK
HHHHHHHCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGVTAKVNSIIIPGINDTHVIEVARKVAGLGADILNCMPYYSTTETVFENIDEPSPEVV
HCCCEEEECEEEECCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCCCCHHHH
SEIQNATAEFLPQMKHCARCRADAVGIIGEINSDEMIQKLAEAASMPKNPGEHRPFIAVS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
SLEGVLVNQHLGEADRFLIYGQDKNGCSVLLDSRAAPPSGGGQERWKALADLLKDCRALL
CCCCEEEHHHCCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHE
VNGAGDSPTKVLNANGIEVMVLEGMIEDAVSGVFNGLSLKHLMKSSQIHACGTSCTGTGG
ECCCCCCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCCCCCCC
GCG
CCC
>Mature Secondary Structure 
TLKLENHPCFNDSARHTFGRIHLPIAPKCNIQCNYCNRKFDCMNENRPGVTSKILSPLQ
EEEECCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHH
AMHYLEQAIALTPSIAVVGIAGPGDPFANPDETMETLRLVRAKYPEMLLCVATNGLDVLP
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
YINELAELKVSHVTITINAIDPEIGSEIYAWVRYNKKMYRDVEAAELLINKQLEALKKLK
HHHHHHHCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGVTAKVNSIIIPGINDTHVIEVARKVAGLGADILNCMPYYSTTETVFENIDEPSPEVV
HCCCEEEECEEEECCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCCCCHHHH
SEIQNATAEFLPQMKHCARCRADAVGIIGEINSDEMIQKLAEAASMPKNPGEHRPFIAVS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
SLEGVLVNQHLGEADRFLIYGQDKNGCSVLLDSRAAPPSGGGQERWKALADLLKDCRALL
CCCCEEEHHHCCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHE
VNGAGDSPTKVLNANGIEVMVLEGMIEDAVSGVFNGLSLKHLMKSSQIHACGTSCTGTGG
ECCCCCCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCCCCCCC
GCG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]