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The map label for this gene is punA [H]

Identifier: 119356537

GI number: 119356537

Start: 802880

End: 803701

Strand: Reverse

Name: punA [H]

Synonym: Cpha266_0702

Alternate gene names: 119356537

Gene position: 803701-802880 (Counterclockwise)

Preceding gene: 119356538

Following gene: 119356536

Centisome position: 25.65

GC content: 50.73

Gene sequence:

>822_bases
ATGATTGCTCAACAGGCTAAAATCCAGGAAGCTGTTACCTTTATCAGAAAAAAAACACAGACTGACTACCCGATCGGCAT
TGTTCTGGGCACAGGCCTCGGTGCTCTTGCAAAAGAGATCGAAGTCGACTTTTCGCTTGATTACGCTGATATCCCGAACT
TTCCTATCTCAACGGTTGAAACCCATCACGGCAAACTGATCTTCGGCACGCTTGCCGGTAAAAAAGTAGTTGCGATGCAG
GGCCGTTTCCATTTCTATGAAGGGTACTCCATGCAGCAGATCGTGTTCCCGATTCGGGTCATGAAACACCTCGGCATTAC
CACCCTCGGCATCACCAATGCCTGCGGAGGCCTTAATCCTTCCTATAAAAAAGGGGATATCATGCTGATTGACGATCACA
TCAACCTGTTAGGCAGCAATCCTCTGATCGGACCCAATAACCCCGAAACAGGATCAAGGTTCCCTGACATGTGCGAACCA
TACTCAAACCGAATCCTCGCCCTTGCCGAACAAGCGGCTCTCGACAACGGCATAAAGGTGCAGCGCGGTGTTTATATAGC
ACTTTCCGGCCCCTGCCTTGAAACACGTGCCGAATATCGCATGCTGCGACTGCTCGGAGCCGATGTTGTCGGCATGTCGA
CCGTTCCGGAGGTTATTGCCGCTGTGCATCAGGGAACCGAAGTGTTCGGCATGTCCATCGTTACCGACGAATGCTTCCCT
GACTGCCTGATGCCCGTCAGCATTGAGGAGATTATTGAGGTTTCCAACCATGCAGAACCCAAAATGACCGCCATCTTCAA
AGCCGTTGTCTCAAACCTCTAA

Upstream 100 bases:

>100_bases
ACTTTTTTACTGTTTCTCTGCCTGATCAACAAGAACATCGGGCATTGATGAATCAAGAAAAACCGGGATATCTTTCATTC
AACCAATAAACCATGGCATC

Downstream 100 bases:

>100_bases
CCCGATCAACATCATTTGACCCATGATAGATGCCATTTCGTTCAATGAGGGTACGCTCCGCTACCTCGATCAGCGTTACC
TTCCGCTCCGTGAAGAGCAT

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MIAQQAKIQEAVTFIRKKTQTDYPIGIVLGTGLGALAKEIEVDFSLDYADIPNFPISTVETHHGKLIFGTLAGKKVVAMQ
GRFHFYEGYSMQQIVFPIRVMKHLGITTLGITNACGGLNPSYKKGDIMLIDDHINLLGSNPLIGPNNPETGSRFPDMCEP
YSNRILALAEQAALDNGIKVQRGVYIALSGPCLETRAEYRMLRLLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
DCLMPVSIEEIIEVSNHAEPKMTAIFKAVVSNL

Sequences:

>Translated_273_residues
MIAQQAKIQEAVTFIRKKTQTDYPIGIVLGTGLGALAKEIEVDFSLDYADIPNFPISTVETHHGKLIFGTLAGKKVVAMQ
GRFHFYEGYSMQQIVFPIRVMKHLGITTLGITNACGGLNPSYKKGDIMLIDDHINLLGSNPLIGPNNPETGSRFPDMCEP
YSNRILALAEQAALDNGIKVQRGVYIALSGPCLETRAEYRMLRLLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
DCLMPVSIEEIIEVSNHAEPKMTAIFKAVVSNL
>Mature_273_residues
MIAQQAKIQEAVTFIRKKTQTDYPIGIVLGTGLGALAKEIEVDFSLDYADIPNFPISTVETHHGKLIFGTLAGKKVVAMQ
GRFHFYEGYSMQQIVFPIRVMKHLGITTLGITNACGGLNPSYKKGDIMLIDDHINLLGSNPLIGPNNPETGSRFPDMCEP
YSNRILALAEQAALDNGIKVQRGVYIALSGPCLETRAEYRMLRLLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
DCLMPVSIEEIIEVSNHAEPKMTAIFKAVVSNL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=256, Percent_Identity=45.3125, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI47132622, Length=251, Percent_Identity=28.6852589641434, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788746, Length=224, Percent_Identity=41.0714285714286, Blast_Score=176, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17541190, Length=268, Percent_Identity=35.8208955223881, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323238, Length=227, Percent_Identity=43.6123348017621, Blast_Score=181, Evalue=8e-47,
Organism=Drosophila melanogaster, GI45552887, Length=238, Percent_Identity=47.8991596638655, Blast_Score=220, Evalue=8e-58,
Organism=Drosophila melanogaster, GI24656093, Length=238, Percent_Identity=47.8991596638655, Blast_Score=220, Evalue=8e-58,
Organism=Drosophila melanogaster, GI24656090, Length=238, Percent_Identity=47.8991596638655, Blast_Score=220, Evalue=9e-58,
Organism=Drosophila melanogaster, GI45552885, Length=238, Percent_Identity=47.8991596638655, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24762376, Length=229, Percent_Identity=35.3711790393013, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29741; Mature: 29741

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAQQAKIQEAVTFIRKKTQTDYPIGIVLGTGLGALAKEIEVDFSLDYADIPNFPISTVE
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHEEEEEECCCCCCCCCCCCEE
THHGKLIFGTLAGKKVVAMQGRFHFYEGYSMQQIVFPIRVMKHLGITTLGITNACGGLNP
CCCCEEEEEECCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHCCCCCCC
SYKKGDIMLIDDHINLLGSNPLIGPNNPETGSRFPDMCEPYSNRILALAEQAALDNGIKV
CCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHCCCCEE
QRGVYIALSGPCLETRAEYRMLRLLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
ECCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEEEEEECHHHCH
DCLMPVSIEEIIEVSNHAEPKMTAIFKAVVSNL
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAQQAKIQEAVTFIRKKTQTDYPIGIVLGTGLGALAKEIEVDFSLDYADIPNFPISTVE
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHEEEEEECCCCCCCCCCCCEE
THHGKLIFGTLAGKKVVAMQGRFHFYEGYSMQQIVFPIRVMKHLGITTLGITNACGGLNP
CCCCEEEEEECCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHCCCCCCC
SYKKGDIMLIDDHINLLGSNPLIGPNNPETGSRFPDMCEPYSNRILALAEQAALDNGIKV
CCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHCCCCEE
QRGVYIALSGPCLETRAEYRMLRLLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
ECCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEEEEEECHHHCH
DCLMPVSIEEIIEVSNHAEPKMTAIFKAVVSNL
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]