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The map label for this gene is purU [H]

Identifier: 119356527

GI number: 119356527

Start: 790664

End: 791530

Strand: Direct

Name: purU [H]

Synonym: Cpha266_0692

Alternate gene names: 119356527

Gene position: 790664-791530 (Clockwise)

Preceding gene: 119356522

Following gene: 119356528

Centisome position: 25.23

GC content: 49.13

Gene sequence:

>867_bases
ATGGAGCTGAACGCCGAGGATGAGATCATCCTGCTGTTGTCGTGCCCGGACAGACCCGGACTTGTTTCACGCATCACCGG
ATTTATCTGTGACCGTGGAGGTAATATTCTCAATTTGAACGAACATGTCGATACCGGAGATAAAACCTTTTTTATAAGGA
TAGCCTGGAGCATCGAGAGTTCGCCACTCTCGCTTGATGAGCTTGAACGAGCGTTCATGCCGCTTGCTCTTGAACTCGGC
GCTGCCTGGAAAATTCGTGAAAGCGGCAAAAAGACCCGTATGGCGATTTTTGTCTCAAAGTACGATCACTGTCTGCAGGA
GATCCTCTGGCGACATAGCATGGGCGAGTTTGCCATCGATATCGCGCTCATCGTTTCGAATCATCCCGACCTGAAACCAC
TTGCAGACCATTACGGGATCGACTATCATCTGTTTGAAACAGACCGTAAGAGTAAAGCTGATGTTGAGCGGGATGAGCTT
GCCCTGCTTGAACAGTACGGTATTGATACCGTTGTGCTGGCCCGATATATGCAGATATTGTCACCGCATTTTGTTGAACG
TTATCCATCGAGAATTATCAACATCCATCACTCATTTCTCCCTGCGTTTGTCGGGGGGAACCCCTATCGGCAGGCATATG
AAAGGGGGGTGAAAATTATTGGCGCCACAAGCCATTATGTTACCGAAGATCTTGATGAAGGTCCGATTATCGAGCAGGAT
ATCATCAGGATAACCCACAAGGACCGGCTTGCAGATCTTATTCGCAAGGGTCGCGATCTCGAACGCATGGTACTTGCCCG
CGCTATCAGGTTCCATGCCGAGCACCGTATTCTGGTCAACGGAAAAAAGACCGTCGTGTTTGAATGA

Upstream 100 bases:

>100_bases
TTGGAGAAATGCCGGATTCTTCCGGGAAAATACATTAAATTTCTTCACACTCACAAAAGAGTGAGGGATGTTGTTTTGAC
GCATGTAAACTGGATACACT

Downstream 100 bases:

>100_bases
AAAGAGGTGTTATACCAATAGCATTGATGTGCGATTCAAGGGCTGCGTGTCGGGAAGAGCAAAAGGATAATATAACGGGC
TTTATCTCATGGCCAACAAC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MELNAEDEIILLLSCPDRPGLVSRITGFICDRGGNILNLNEHVDTGDKTFFIRIAWSIESSPLSLDELERAFMPLALELG
AAWKIRESGKKTRMAIFVSKYDHCLQEILWRHSMGEFAIDIALIVSNHPDLKPLADHYGIDYHLFETDRKSKADVERDEL
ALLEQYGIDTVVLARYMQILSPHFVERYPSRIINIHHSFLPAFVGGNPYRQAYERGVKIIGATSHYVTEDLDEGPIIEQD
IIRITHKDRLADLIRKGRDLERMVLARAIRFHAEHRILVNGKKTVVFE

Sequences:

>Translated_288_residues
MELNAEDEIILLLSCPDRPGLVSRITGFICDRGGNILNLNEHVDTGDKTFFIRIAWSIESSPLSLDELERAFMPLALELG
AAWKIRESGKKTRMAIFVSKYDHCLQEILWRHSMGEFAIDIALIVSNHPDLKPLADHYGIDYHLFETDRKSKADVERDEL
ALLEQYGIDTVVLARYMQILSPHFVERYPSRIINIHHSFLPAFVGGNPYRQAYERGVKIIGATSHYVTEDLDEGPIIEQD
IIRITHKDRLADLIRKGRDLERMVLARAIRFHAEHRILVNGKKTVVFE
>Mature_288_residues
MELNAEDEIILLLSCPDRPGLVSRITGFICDRGGNILNLNEHVDTGDKTFFIRIAWSIESSPLSLDELERAFMPLALELG
AAWKIRESGKKTRMAIFVSKYDHCLQEILWRHSMGEFAIDIALIVSNHPDLKPLADHYGIDYHLFETDRKSKADVERDEL
ALLEQYGIDTVVLARYMQILSPHFVERYPSRIINIHHSFLPAFVGGNPYRQAYERGVKIIGATSHYVTEDLDEGPIIEQD
IIRITHKDRLADLIRKGRDLERMVLARAIRFHAEHRILVNGKKTVVFE

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=199, Percent_Identity=32.6633165829146, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI209869995, Length=199, Percent_Identity=32.6633165829146, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI209869993, Length=199, Percent_Identity=32.6633165829146, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=40.2877697841727, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1788846, Length=188, Percent_Identity=30.8510638297872, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=170, Percent_Identity=35.8823529411765, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24582400, Length=206, Percent_Identity=30.0970873786408, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33056; Mature: 33056

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNAEDEIILLLSCPDRPGLVSRITGFICDRGGNILNLNEHVDTGDKTFFIRIAWSIES
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEEEEEECC
SPLSLDELERAFMPLALELGAAWKIRESGKKTRMAIFVSKYDHCLQEILWRHSMGEFAID
CCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEE
IALIVSNHPDLKPLADHYGIDYHLFETDRKSKADVERDELALLEQYGIDTVVLARYMQIL
EEEEECCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
SPHFVERYPSRIINIHHSFLPAFVGGNPYRQAYERGVKIIGATSHYVTEDLDEGPIIEQD
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCHHHHHCCCCCCCCCHHH
IIRITHKDRLADLIRKGRDLERMVLARAIRFHAEHRILVNGKKTVVFE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC
>Mature Secondary Structure
MELNAEDEIILLLSCPDRPGLVSRITGFICDRGGNILNLNEHVDTGDKTFFIRIAWSIES
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEEEEEECC
SPLSLDELERAFMPLALELGAAWKIRESGKKTRMAIFVSKYDHCLQEILWRHSMGEFAID
CCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEE
IALIVSNHPDLKPLADHYGIDYHLFETDRKSKADVERDELALLEQYGIDTVVLARYMQIL
EEEEECCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
SPHFVERYPSRIINIHHSFLPAFVGGNPYRQAYERGVKIIGATSHYVTEDLDEGPIIEQD
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCHHHHHCCCCCCCCCHHH
IIRITHKDRLADLIRKGRDLERMVLARAIRFHAEHRILVNGKKTVVFE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]