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The map label for this gene is recJ [H]

Identifier: 119356522

GI number: 119356522

Start: 784797

End: 786521

Strand: Direct

Name: recJ [H]

Synonym: Cpha266_0687

Alternate gene names: 119356522

Gene position: 784797-786521 (Clockwise)

Preceding gene: 119356520

Following gene: 119356527

Centisome position: 25.04

GC content: 47.3

Gene sequence:

>1725_bases
ATGAAACGTTACCGCTGGAAGCTGTTGATGCCGGATGAGCAGATGGTCAGCCATCTTTCCGGAGCTATCAATGTCAGTAC
CCCTCTTTCCAGGGCGTTATGCAATCGCGGTATCGCTACCTTTGATGAAGCGAAAGATTTTTTTCGCCCGTCGATTCATC
AGTTGCCATCACCCTTTCTTTTCAAGGATATGCTGCGAGCTGTTGACCGTGTTGCGGATGCCTTGGAGAAGCGTGAAAAA
ATAATGCTCTATGGCGATTATGATGTTGACGGAACAACAGGAACGGCTCTATTATACCTTTTTTTACAAGAGCGGGGAGC
GGAAGTTTCCTACTACATCAACGATCGGTTTACCGAAGGATACGGGTTATCCCGGGAAGGTATTGAGACTGCAGTTGCCC
GGAACACAGGGCTCATTATTACCGTTGATTGCGGGATCCGTGCTGAAGAGCAGATCCTGTTGTGCAATGATCATGGTATT
GATGTTATTATCTGTGATCATCATGAACCTCATGAGCTTCCGCCGGCATATGCTATTCTCAATCCAAAGCTTGGTGATTG
TAGCTATCCTTTCAGAGAACTTTGTGGTTGCGGTGTGGCATTCAGGTTTATACAAGCAATAGCCGAGCAACTTGATACGG
GTATGGAGAGCTGGCAGAAATATTTTGATCTGGTTGCCATTGCAACTGCCGCTGATATGGTTGTTCTGGAAAAGGAAAAC
AGAACGTATGTGCGTGAAGGATTGCAGAGAATAAGAACCAGTCCAAGGAGCAGCCTTGCCCAGATGCTTGGCATTATGAA
TGTTGATGCCGATATGATGTCGATGTTTACCATAGCGTTCGGAATTGCACCAAGAATAAATGCCGCAGGGCGAATGGAGT
CGGCAACGCTGGCCGTGAAGTGGCTTCTTTCCGATTCGGTTGAGGATTCACAGCAACATGCCGGAGAGCTCGAGCAGTTG
AACGCCCTGCGCAGAAAAATCGATGCCGAGATTTTCATTCAGGCAGAAAAAATGATTGCCGGTCATTTTGCATCCTACTG
TTCTTCGATTGTTCTCTATGACGAGGAGTGGCATCTTGGTGTTCTTGGTATTGTCGCCTCGAAAATGCTTGAAAAATACT
ATCTGCCCACCGTGATCATGGGTAACATGAATGGACTTGTCAAAGGGTCGGTGAGAAGCGTGGACGGGCTTAATATCTAT
GAAGTGCTTCAGGAGTGCGGTGAGTATCTTGAGCAGTTTGGGGGGCATAATCAGGCCGCAGGTATTACACTCAGACCGGA
AAACCTTGCCGGGTTTCGAAAAAGATTTGATGAGATCTGCCGGGATCTTCAGCCAGTCGAACTTCGTCAGAAAGAGGTTC
TGATCGATTCCGTCCTTGCCCTTGAAGATGTTACCGATAAATTCATGAACGTGCTTGCAGAGTTTGCTCCCTACGGGTAT
GGAAACCGGGAGCCGCTGTTTGTTTCAGACCAGGTTCTCCTGCAAGGACGTCCCCGTTTGCTCAAGGAACGGCATGTCAA
GTTTTCAGTCAGGGATAAACATGACCGGATATTTGATGTTATAGGATTTGATCGTCGCGATATCTATGAGGATCTTGCTG
CTTCGAATACTCCTGTTGTTGCCATGGTCTACTCTCTTGAAAAGAGAAGCTGGAATAACCGTGAACATTGGCAGATCAGG
CTTAAAGACCTCTCGATTACTCAGGATCAGCCATGGAAGGAGTAG

Upstream 100 bases:

>100_bases
TCAACAGCAGCAATCTGGTTGTCAAGAGTTTTTTGCTCAGAGCAGTTCAAGTATTGTATATTCATCTGCATTACTCTTCG
TATTTCTATCCCCTGAACTG

Downstream 100 bases:

>100_bases
AATCTTCAGCAGAGGGAAGACTTTTTTCATGTCTTGTTCTGCCGAGAATACCAGCAAGAGACGAAATGGCTGAAATTCCG
AAGAAAATAAGCGATAATGC

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKRYRWKLLMPDEQMVSHLSGAINVSTPLSRALCNRGIATFDEAKDFFRPSIHQLPSPFLFKDMLRAVDRVADALEKREK
IMLYGDYDVDGTTGTALLYLFLQERGAEVSYYINDRFTEGYGLSREGIETAVARNTGLIITVDCGIRAEEQILLCNDHGI
DVIICDHHEPHELPPAYAILNPKLGDCSYPFRELCGCGVAFRFIQAIAEQLDTGMESWQKYFDLVAIATAADMVVLEKEN
RTYVREGLQRIRTSPRSSLAQMLGIMNVDADMMSMFTIAFGIAPRINAAGRMESATLAVKWLLSDSVEDSQQHAGELEQL
NALRRKIDAEIFIQAEKMIAGHFASYCSSIVLYDEEWHLGVLGIVASKMLEKYYLPTVIMGNMNGLVKGSVRSVDGLNIY
EVLQECGEYLEQFGGHNQAAGITLRPENLAGFRKRFDEICRDLQPVELRQKEVLIDSVLALEDVTDKFMNVLAEFAPYGY
GNREPLFVSDQVLLQGRPRLLKERHVKFSVRDKHDRIFDVIGFDRRDIYEDLAASNTPVVAMVYSLEKRSWNNREHWQIR
LKDLSITQDQPWKE

Sequences:

>Translated_574_residues
MKRYRWKLLMPDEQMVSHLSGAINVSTPLSRALCNRGIATFDEAKDFFRPSIHQLPSPFLFKDMLRAVDRVADALEKREK
IMLYGDYDVDGTTGTALLYLFLQERGAEVSYYINDRFTEGYGLSREGIETAVARNTGLIITVDCGIRAEEQILLCNDHGI
DVIICDHHEPHELPPAYAILNPKLGDCSYPFRELCGCGVAFRFIQAIAEQLDTGMESWQKYFDLVAIATAADMVVLEKEN
RTYVREGLQRIRTSPRSSLAQMLGIMNVDADMMSMFTIAFGIAPRINAAGRMESATLAVKWLLSDSVEDSQQHAGELEQL
NALRRKIDAEIFIQAEKMIAGHFASYCSSIVLYDEEWHLGVLGIVASKMLEKYYLPTVIMGNMNGLVKGSVRSVDGLNIY
EVLQECGEYLEQFGGHNQAAGITLRPENLAGFRKRFDEICRDLQPVELRQKEVLIDSVLALEDVTDKFMNVLAEFAPYGY
GNREPLFVSDQVLLQGRPRLLKERHVKFSVRDKHDRIFDVIGFDRRDIYEDLAASNTPVVAMVYSLEKRSWNNREHWQIR
LKDLSITQDQPWKE
>Mature_574_residues
MKRYRWKLLMPDEQMVSHLSGAINVSTPLSRALCNRGIATFDEAKDFFRPSIHQLPSPFLFKDMLRAVDRVADALEKREK
IMLYGDYDVDGTTGTALLYLFLQERGAEVSYYINDRFTEGYGLSREGIETAVARNTGLIITVDCGIRAEEQILLCNDHGI
DVIICDHHEPHELPPAYAILNPKLGDCSYPFRELCGCGVAFRFIQAIAEQLDTGMESWQKYFDLVAIATAADMVVLEKEN
RTYVREGLQRIRTSPRSSLAQMLGIMNVDADMMSMFTIAFGIAPRINAAGRMESATLAVKWLLSDSVEDSQQHAGELEQL
NALRRKIDAEIFIQAEKMIAGHFASYCSSIVLYDEEWHLGVLGIVASKMLEKYYLPTVIMGNMNGLVKGSVRSVDGLNIY
EVLQECGEYLEQFGGHNQAAGITLRPENLAGFRKRFDEICRDLQPVELRQKEVLIDSVLALEDVTDKFMNVLAEFAPYGY
GNREPLFVSDQVLLQGRPRLLKERHVKFSVRDKHDRIFDVIGFDRRDIYEDLAASNTPVVAMVYSLEKRSWNNREHWQIR
LKDLSITQDQPWKE

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=502, Percent_Identity=33.2669322709163, Blast_Score=262, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 65297; Mature: 65297

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYRWKLLMPDEQMVSHLSGAINVSTPLSRALCNRGIATFDEAKDFFRPSIHQLPSPFL
CCCEEEEEECCHHHHHHHHCCCEECCCHHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCHH
FKDMLRAVDRVADALEKREKIMLYGDYDVDGTTGTALLYLFLQERGAEVSYYINDRFTEG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
YGLSREGIETAVARNTGLIITVDCGIRAEEQILLCNDHGIDVIICDHHEPHELPPAYAIL
CCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEE
NPKLGDCSYPFRELCGCGVAFRFIQAIAEQLDTGMESWQKYFDLVAIATAADMVVLEKEN
CCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
RTYVREGLQRIRTSPRSSLAQMLGIMNVDADMMSMFTIAFGIAPRINAAGRMESATLAVK
CHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
WLLSDSVEDSQQHAGELEQLNALRRKIDAEIFIQAEKMIAGHFASYCSSIVLYDEEWHLG
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHCEEEEECCCCCCH
VLGIVASKMLEKYYLPTVIMGNMNGLVKGSVRSVDGLNIYEVLQECGEYLEQFGGHNQAA
HHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GITLRPENLAGFRKRFDEICRDLQPVELRQKEVLIDSVLALEDVTDKFMNVLAEFAPYGY
CEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GNREPLFVSDQVLLQGRPRLLKERHVKFSVRDKHDRIFDVIGFDRRDIYEDLAASNTPVV
CCCCCEEECCHHHCCCCHHHHHHHCCEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCEE
AMVYSLEKRSWNNREHWQIRLKDLSITQDQPWKE
EEEEEHHHCCCCCCCEEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKRYRWKLLMPDEQMVSHLSGAINVSTPLSRALCNRGIATFDEAKDFFRPSIHQLPSPFL
CCCEEEEEECCHHHHHHHHCCCEECCCHHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCHH
FKDMLRAVDRVADALEKREKIMLYGDYDVDGTTGTALLYLFLQERGAEVSYYINDRFTEG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
YGLSREGIETAVARNTGLIITVDCGIRAEEQILLCNDHGIDVIICDHHEPHELPPAYAIL
CCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEE
NPKLGDCSYPFRELCGCGVAFRFIQAIAEQLDTGMESWQKYFDLVAIATAADMVVLEKEN
CCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
RTYVREGLQRIRTSPRSSLAQMLGIMNVDADMMSMFTIAFGIAPRINAAGRMESATLAVK
CHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
WLLSDSVEDSQQHAGELEQLNALRRKIDAEIFIQAEKMIAGHFASYCSSIVLYDEEWHLG
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHCEEEEECCCCCCH
VLGIVASKMLEKYYLPTVIMGNMNGLVKGSVRSVDGLNIYEVLQECGEYLEQFGGHNQAA
HHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GITLRPENLAGFRKRFDEICRDLQPVELRQKEVLIDSVLALEDVTDKFMNVLAEFAPYGY
CEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GNREPLFVSDQVLLQGRPRLLKERHVKFSVRDKHDRIFDVIGFDRRDIYEDLAASNTPVV
CCCCCEEECCHHHCCCCHHHHHHHCCEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCEE
AMVYSLEKRSWNNREHWQIRLKDLSITQDQPWKE
EEEEEHHHCCCCCCCEEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]