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The map label for this gene is appA [H]

Identifier: 119356403

GI number: 119356403

Start: 634030

End: 635856

Strand: Direct

Name: appA [H]

Synonym: Cpha266_0567

Alternate gene names: 119356403

Gene position: 634030-635856 (Clockwise)

Preceding gene: 119356402

Following gene: 119356404

Centisome position: 20.23

GC content: 45.81

Gene sequence:

>1827_bases
ATGACCATGTTTACTAAACGGGAAGTAACAGTGCCTGCATGTTCTAATAGCCGGCCATTTCGTTTCTCGCTGAGCGCGTT
AATTCTTTTTATGACGGTGCTGGCTTCCACTTCCTGCAGCGAAAAAAAACAGGGCGATGACATCGGAAGCAAAGGTCGTG
CAGCAAAAGATTCAACGCTGGTTATTGCAATGCTGGGGGATGCTGATTATTTGAATCCCGTGCTTGGAACAACGGTGACC
TCGAACAATATTTTCAGTCTCATCTATCCGGGTCTCTTGCAAAGCGAGTTTGATACGACCACTGGTTTGCTGAATTTTAT
CGCGCTTGAAAAACGGTTGAGGCAAACAGGCACCGGTACCGGGAAAAAAACGCCACGCGCTGCTCTTGCAAAAACCTGGC
GGATGGCTCCGGATCATAAATCCATTACCTATATTCTCAGAAACAACGCATTCTGGAACGATGGCAAGCCGATTGTTTCC
GGAGATTTTAAGTTTTCCTATAAGCTGTATGGTAATCCCGTTATTGCAAGTGCTCGTCAGCAGTACCTTTCCGAGCTGAT
CGGCGCTGAAACCGGGCAGGTTGATTTTCGGAGGGCTATCGAAACACCTGATGACACGACATTGATTTTCAGGTTTCATA
AGCCTGTTTCTGAACAGCTTGCGCTTTTTCATACCTCGCTGACTCCTTTGCCTTCACATTACTGGAGGTCGGTAAAGCCG
GAGGATTTCAGAAGCTCGCCACTCAATCAGTTACCGCTTGGCGCAGGGCCATACAAGTTGCAGGTTTGGCGGCAGCAGCA
GGAAATTGTGCTTGCTTCAAACAAGAGAAGTAATCTGCCTAAGCCAGGCAATATCCCCTATATTTCCTATCGTGTTGTGC
CGGATTATACGGTAAGATTAACTCAGCTTCAGACGAATGCTGTTGATGTTGTTGAAAATATTAAACCTGAGGATTTTCAG
GGGGTTCTGAAATCCAACGCTGCAATTGAGATTAAAACTGTCGGACTCAGGGTTTTTGACTATGTAGGCTGGTCAAATAT
TGATCAGGCCGAGTATCACAAAACCGGAAAAATCAAACCCCATCCGCTTTTTGGTTCTGCACAGGTTCGCCTTGCGCTTA
CAACGGCTATTGACAGAGAGTCGATCATTGATGGTTATCTCAAGAGCTATGGCGTTCTTTGCAATACCGATATTTCACCT
TCGCTGAAATGGGCGTACAATAGAGCTATTCTTGCTCATCAGTTCGATCCCGCAAAAGCTTCGGCACTGCTCAAAGCCGA
AGGCTGGCTTCCGGGACCTGACGGTATTCTTCGAAAAAACGGAAGGAAATTCAGTTTTGTACTTTACACCAATTCCGGCA
ATGCCCGGAGAAATTATGCGAGCGTCATCATCCAGCAGAATCTGAAGGCGATCGGCATTGACTGCAAGCTTGACGTTCAG
GAATCCAATGTCTTTTTTGAAAATCTTCAGTCGAGAAAACTTGATGCATGGATGGCCGGCTGGTCTATAGGGCTTGAAAT
TGATCCTCTTGATGTCTGGGGTTCCGATCTCAAAAAAAGCCGATTTAATTTTACCGGCTATCAAAACCCGAGAATTGACG
GACTTTGTGAGCTTGCGAAACAGAAGATGGATCCACTGGAAGCGAAAGCGTACTGGATGGAATATCAGCAAATTCTTCAT
CGCGATCAGCCGGTCACATTTTTGTACTGGATAAGGGAAACGCAAGGTTTCAATAAAAGAATTCAGGGCGAAGAGCTTAA
TATTTCAGGAACCTTTTACAATATTGACGACTGGACTCTTAACCCTTCGGCAACTGTGGCTCTTTAA

Upstream 100 bases:

>100_bases
AGCCCTTAATTTCCTTTTAAAGCACTATTACGATTATGTGCCGGCAGAAGCTCTTACAAAGAGAATCAGAACGGCGATAG
ATCAATGACTCTTCAAAACG

Downstream 100 bases:

>100_bases
GATGATACCATGCTGACTTACATTATTAAACGGCTGCTGATAGCCGTACCGCTAATTTTTGGTGTACTCACACTTACGTT
TTTTATCATCAGGCTCGCTC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MTMFTKREVTVPACSNSRPFRFSLSALILFMTVLASTSCSEKKQGDDIGSKGRAAKDSTLVIAMLGDADYLNPVLGTTVT
SNNIFSLIYPGLLQSEFDTTTGLLNFIALEKRLRQTGTGTGKKTPRAALAKTWRMAPDHKSITYILRNNAFWNDGKPIVS
GDFKFSYKLYGNPVIASARQQYLSELIGAETGQVDFRRAIETPDDTTLIFRFHKPVSEQLALFHTSLTPLPSHYWRSVKP
EDFRSSPLNQLPLGAGPYKLQVWRQQQEIVLASNKRSNLPKPGNIPYISYRVVPDYTVRLTQLQTNAVDVVENIKPEDFQ
GVLKSNAAIEIKTVGLRVFDYVGWSNIDQAEYHKTGKIKPHPLFGSAQVRLALTTAIDRESIIDGYLKSYGVLCNTDISP
SLKWAYNRAILAHQFDPAKASALLKAEGWLPGPDGILRKNGRKFSFVLYTNSGNARRNYASVIIQQNLKAIGIDCKLDVQ
ESNVFFENLQSRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFTGYQNPRIDGLCELAKQKMDPLEAKAYWMEYQQILH
RDQPVTFLYWIRETQGFNKRIQGEELNISGTFYNIDDWTLNPSATVAL

Sequences:

>Translated_608_residues
MTMFTKREVTVPACSNSRPFRFSLSALILFMTVLASTSCSEKKQGDDIGSKGRAAKDSTLVIAMLGDADYLNPVLGTTVT
SNNIFSLIYPGLLQSEFDTTTGLLNFIALEKRLRQTGTGTGKKTPRAALAKTWRMAPDHKSITYILRNNAFWNDGKPIVS
GDFKFSYKLYGNPVIASARQQYLSELIGAETGQVDFRRAIETPDDTTLIFRFHKPVSEQLALFHTSLTPLPSHYWRSVKP
EDFRSSPLNQLPLGAGPYKLQVWRQQQEIVLASNKRSNLPKPGNIPYISYRVVPDYTVRLTQLQTNAVDVVENIKPEDFQ
GVLKSNAAIEIKTVGLRVFDYVGWSNIDQAEYHKTGKIKPHPLFGSAQVRLALTTAIDRESIIDGYLKSYGVLCNTDISP
SLKWAYNRAILAHQFDPAKASALLKAEGWLPGPDGILRKNGRKFSFVLYTNSGNARRNYASVIIQQNLKAIGIDCKLDVQ
ESNVFFENLQSRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFTGYQNPRIDGLCELAKQKMDPLEAKAYWMEYQQILH
RDQPVTFLYWIRETQGFNKRIQGEELNISGTFYNIDDWTLNPSATVAL
>Mature_607_residues
TMFTKREVTVPACSNSRPFRFSLSALILFMTVLASTSCSEKKQGDDIGSKGRAAKDSTLVIAMLGDADYLNPVLGTTVTS
NNIFSLIYPGLLQSEFDTTTGLLNFIALEKRLRQTGTGTGKKTPRAALAKTWRMAPDHKSITYILRNNAFWNDGKPIVSG
DFKFSYKLYGNPVIASARQQYLSELIGAETGQVDFRRAIETPDDTTLIFRFHKPVSEQLALFHTSLTPLPSHYWRSVKPE
DFRSSPLNQLPLGAGPYKLQVWRQQQEIVLASNKRSNLPKPGNIPYISYRVVPDYTVRLTQLQTNAVDVVENIKPEDFQG
VLKSNAAIEIKTVGLRVFDYVGWSNIDQAEYHKTGKIKPHPLFGSAQVRLALTTAIDRESIIDGYLKSYGVLCNTDISPS
LKWAYNRAILAHQFDPAKASALLKAEGWLPGPDGILRKNGRKFSFVLYTNSGNARRNYASVIIQQNLKAIGIDCKLDVQE
SNVFFENLQSRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFTGYQNPRIDGLCELAKQKMDPLEAKAYWMEYQQILHR
DQPVTFLYWIRETQGFNKRIQGEELNISGTFYNIDDWTLNPSATVAL

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=461, Percent_Identity=23.8611713665944, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789887, Length=461, Percent_Identity=25.5965292841649, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787762, Length=542, Percent_Identity=23.0627306273063, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787052, Length=326, Percent_Identity=24.2331288343558, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 68473; Mature: 68342

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMFTKREVTVPACSNSRPFRFSLSALILFMTVLASTSCSEKKQGDDIGSKGRAAKDSTL
CCCEECCEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCEE
VIAMLGDADYLNPVLGTTVTSNNIFSLIYPGLLQSEFDTTTGLLNFIALEKRLRQTGTGT
EEEEECCCHHCCHHHCCEEECCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
GKKTPRAALAKTWRMAPDHKSITYILRNNAFWNDGKPIVSGDFKFSYKLYGNPVIASARQ
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCEEECCEEEEEEEECCCHHHHHHH
QYLSELIGAETGQVDFRRAIETPDDTTLIFRFHKPVSEQLALFHTSLTPLPSHYWRSVKP
HHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHCCCCC
EDFRSSPLNQLPLGAGPYKLQVWRQQQEIVLASNKRSNLPKPGNIPYISYRVVPDYTVRL
CCHHCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEE
TQLQTNAVDVVENIKPEDFQGVLKSNAAIEIKTVGLRVFDYVGWSNIDQAEYHKTGKIKP
EEEECHHHHHHHCCCCHHHHHHHHCCCEEEEEEECEEEEEECCCCCCCHHHHCCCCCCCC
HPLFGSAQVRLALTTAIDRESIIDGYLKSYGVLCNTDISPSLKWAYNRAILAHQFDPAKA
CCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEECCCCCCHHHHHCCEEEEECCCCHHH
SALLKAEGWLPGPDGILRKNGRKFSFVLYTNSGNARRNYASVIIQQNLKAIGIDCKLDVQ
HEEEEECCCCCCCCHHHHCCCCEEEEEEEECCCCCCHHHHHHHEECCCEEEECEEEEEEC
ESNVFFENLQSRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFTGYQNPRIDGLCELAK
CCCHHHHHHHHCCCHHEECCEEECEEECCHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHH
QKMDPLEAKAYWMEYQQILHRDQPVTFLYWIRETQGFNKRIQGEELNISGTFYNIDDWTL
HHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEEEECCCEEE
NPSATVAL
CCCCEEEC
>Mature Secondary Structure 
TMFTKREVTVPACSNSRPFRFSLSALILFMTVLASTSCSEKKQGDDIGSKGRAAKDSTL
CCEECCEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCEE
VIAMLGDADYLNPVLGTTVTSNNIFSLIYPGLLQSEFDTTTGLLNFIALEKRLRQTGTGT
EEEEECCCHHCCHHHCCEEECCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
GKKTPRAALAKTWRMAPDHKSITYILRNNAFWNDGKPIVSGDFKFSYKLYGNPVIASARQ
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCEEECCEEEEEEEECCCHHHHHHH
QYLSELIGAETGQVDFRRAIETPDDTTLIFRFHKPVSEQLALFHTSLTPLPSHYWRSVKP
HHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHCCCCC
EDFRSSPLNQLPLGAGPYKLQVWRQQQEIVLASNKRSNLPKPGNIPYISYRVVPDYTVRL
CCHHCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEE
TQLQTNAVDVVENIKPEDFQGVLKSNAAIEIKTVGLRVFDYVGWSNIDQAEYHKTGKIKP
EEEECHHHHHHHCCCCHHHHHHHHCCCEEEEEEECEEEEEECCCCCCCHHHHCCCCCCCC
HPLFGSAQVRLALTTAIDRESIIDGYLKSYGVLCNTDISPSLKWAYNRAILAHQFDPAKA
CCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEECCCCCCHHHHHCCEEEEECCCCHHH
SALLKAEGWLPGPDGILRKNGRKFSFVLYTNSGNARRNYASVIIQQNLKAIGIDCKLDVQ
HEEEEECCCCCCCCHHHHCCCCEEEEEEEECCCCCCHHHHHHHEECCCEEEECEEEEEEC
ESNVFFENLQSRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFTGYQNPRIDGLCELAK
CCCHHHHHHHHCCCHHEECCEEECEEECCHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHH
QKMDPLEAKAYWMEYQQILHRDQPVTFLYWIRETQGFNKRIQGEELNISGTFYNIDDWTL
HHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEEEECCCEEE
NPSATVAL
CCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]