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The map label for this gene is ptsI [H]

Identifier: 119356180

GI number: 119356180

Start: 372862

End: 374670

Strand: Reverse

Name: ptsI [H]

Synonym: Cpha266_0341

Alternate gene names: 119356180

Gene position: 374670-372862 (Counterclockwise)

Preceding gene: 119356181

Following gene: 119356177

Centisome position: 11.96

GC content: 46.27

Gene sequence:

>1809_bases
ATGCCTGAACAGAAAAAAAACACACTGAAGGGAGTTCTTCCCTCAAAAAAACAGAGCCGTTCAAATCGAAGCGATGGCGG
AAATATAAAGCCCCATGAAGCAACGTTCAATGGCATAGGGACTTCACGAGGAATTGCCATCGGTGAATGTTATGCTTATG
AAAAGGAACAATATGAGCATGAAATCACCTCTCTCGACAACGATAACATCGAAGCGGAAATCGAGCGGTTTATTGAAGCA
GTCCATCGCTCAGAAAAGGAACTGAAAAAAATAGAACGGGTCACGACAAGAAAACTCGGCAAGGGTTATGCGAACATTTT
TCAGGCCCAGATCATGATGCTCCAGGACCCGATGCTCATCGACAGCATCAGCCTTCGTATCAGATCAGAAATGATACCAG
CACATCTTGTTATTGAAGAGGAGTTCGGACACTATCACGAAAAGTTCAAAAGCTCAGATGATGTTGTTTTTCAGGAGCGG
GCTGATGATTTTTTTGATATAAAAAACAGGATTCTCAGAAATCTCCATGTCCGCAAAATGCTCTCGTGGATACCCGAAGG
CACCATTGTGGTTTCCGATCACTTCTGCCCCGGAGACATTATTCTGCTCAGCCGGAGCAATGTCAAGGGCTTTCTCAGCG
ATTCAGGCGGAAAAACTTCTCATATCTCTTTGATCTGTAAATCGCTGAACATCCCTATGGTGGCAGGGCTCGGCAATTTT
TCACAGAATGCTGTTTCAGGCACACAGATGATTATTGACGGGAGTTCCGGTACCGTTATCATCCATCCCGAAGAAAAAAC
CATTCAGCTCTATCAGGAAAAACAGAAAGCGGAAAAACAGAGCAATGCCGATGCATCTCTCACGGCAACGCTCCCGGCCA
GTACAAAATGCGGCGTAAGAATAAACTTTTTTTCAAACATCGATTTCAAGGAAGAACTCCTGGGGCTCAAAGAAACAGGA
TCAGAAGGTATAGGGCTTTTCAGGAGTGAAAATCTTTTTATTGACGGAACAAAGGTACCTGGGGAATCGGAACAGTACCA
CTATTACCGGGAAATGTCGGAAACAGTTGCTCCGCAGCCCTTTGTTGTCCGACTGTTTGATATAGGGGGCGATAAACTTA
TTTATTCACCAATCAGGGAGCCGAACCCCAATCTTGGATGGAGAGGAATAAGAATTCTCATTGATGTACCTGAAATTCTC
GAAAATCAGCTCAGAGCGGTTATCAGGGCAAACGCCCTCGGCAACATCAATATTCTTATACCGATGATCACCTCTCTCGA
CGAGATCATGACCATTTGCGCCATGGTTGACAGACTGCATGCCGAGGTCATTGAGCAAACTGGCGTCGCCGGCGAAAAAC
CTGAACTCGGCGCAATGATCGAGGTGCCTGCGGCAGTCGAGATTATCGACGAGATTACGAAATGCGTAGATTTTATCAGT
CTCGGCACCAATGATCTTACACAGTATACCCTTGCCGTTGACCGCAACAACGTCATTGTTCAGGATCTCTTTGAAAAATT
TCACCCTGCCATTATCCGGCAGCTTCACAGAGTAATTGCAACCGCCCAGAAAAATCACTGCCGTGTCGCGCTGTGCGGCG
ACATGGGTTCAGACCCGCTTGCCCTCCCCTTTCTGATAGGGTGCGGCTTGAGAAAGTTCAGTGTTGTAAGTGCTGATATT
GCGAATCTTAAACGGTTCGTATCGCGCTACAGCGTTGCTGAAACAGAGGCGCTGGCGCTTGAATGCATCAAACTCGATTC
GGCACAAAAAATAAAAGCCTGCCTTGAAACATTTCAGGCCGAACACTAA

Upstream 100 bases:

>100_bases
AACGCAATCTACCCCCTGAAGCACCCGCACTCCAGAAAACCCAAATGAGCGCAAACACCTTCATTAACGCCGACGATGCA
CCTTTTTAAGACTGTGCCCT

Downstream 100 bases:

>100_bases
AAGAGGGTTTTTGGATACCATTCACGCATCCTGACTGACAAAATCACATAGAGGCAGGCGCTTATTTTCCGAAACCTTGT
GCTGCCCGGTTGCCATGCGC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MPEQKKNTLKGVLPSKKQSRSNRSDGGNIKPHEATFNGIGTSRGIAIGECYAYEKEQYEHEITSLDNDNIEAEIERFIEA
VHRSEKELKKIERVTTRKLGKGYANIFQAQIMMLQDPMLIDSISLRIRSEMIPAHLVIEEEFGHYHEKFKSSDDVVFQER
ADDFFDIKNRILRNLHVRKMLSWIPEGTIVVSDHFCPGDIILLSRSNVKGFLSDSGGKTSHISLICKSLNIPMVAGLGNF
SQNAVSGTQMIIDGSSGTVIIHPEEKTIQLYQEKQKAEKQSNADASLTATLPASTKCGVRINFFSNIDFKEELLGLKETG
SEGIGLFRSENLFIDGTKVPGESEQYHYYREMSETVAPQPFVVRLFDIGGDKLIYSPIREPNPNLGWRGIRILIDVPEIL
ENQLRAVIRANALGNINILIPMITSLDEIMTICAMVDRLHAEVIEQTGVAGEKPELGAMIEVPAAVEIIDEITKCVDFIS
LGTNDLTQYTLAVDRNNVIVQDLFEKFHPAIIRQLHRVIATAQKNHCRVALCGDMGSDPLALPFLIGCGLRKFSVVSADI
ANLKRFVSRYSVAETEALALECIKLDSAQKIKACLETFQAEH

Sequences:

>Translated_602_residues
MPEQKKNTLKGVLPSKKQSRSNRSDGGNIKPHEATFNGIGTSRGIAIGECYAYEKEQYEHEITSLDNDNIEAEIERFIEA
VHRSEKELKKIERVTTRKLGKGYANIFQAQIMMLQDPMLIDSISLRIRSEMIPAHLVIEEEFGHYHEKFKSSDDVVFQER
ADDFFDIKNRILRNLHVRKMLSWIPEGTIVVSDHFCPGDIILLSRSNVKGFLSDSGGKTSHISLICKSLNIPMVAGLGNF
SQNAVSGTQMIIDGSSGTVIIHPEEKTIQLYQEKQKAEKQSNADASLTATLPASTKCGVRINFFSNIDFKEELLGLKETG
SEGIGLFRSENLFIDGTKVPGESEQYHYYREMSETVAPQPFVVRLFDIGGDKLIYSPIREPNPNLGWRGIRILIDVPEIL
ENQLRAVIRANALGNINILIPMITSLDEIMTICAMVDRLHAEVIEQTGVAGEKPELGAMIEVPAAVEIIDEITKCVDFIS
LGTNDLTQYTLAVDRNNVIVQDLFEKFHPAIIRQLHRVIATAQKNHCRVALCGDMGSDPLALPFLIGCGLRKFSVVSADI
ANLKRFVSRYSVAETEALALECIKLDSAQKIKACLETFQAEH
>Mature_601_residues
PEQKKNTLKGVLPSKKQSRSNRSDGGNIKPHEATFNGIGTSRGIAIGECYAYEKEQYEHEITSLDNDNIEAEIERFIEAV
HRSEKELKKIERVTTRKLGKGYANIFQAQIMMLQDPMLIDSISLRIRSEMIPAHLVIEEEFGHYHEKFKSSDDVVFQERA
DDFFDIKNRILRNLHVRKMLSWIPEGTIVVSDHFCPGDIILLSRSNVKGFLSDSGGKTSHISLICKSLNIPMVAGLGNFS
QNAVSGTQMIIDGSSGTVIIHPEEKTIQLYQEKQKAEKQSNADASLTATLPASTKCGVRINFFSNIDFKEELLGLKETGS
EGIGLFRSENLFIDGTKVPGESEQYHYYREMSETVAPQPFVVRLFDIGGDKLIYSPIREPNPNLGWRGIRILIDVPEILE
NQLRAVIRANALGNINILIPMITSLDEIMTICAMVDRLHAEVIEQTGVAGEKPELGAMIEVPAAVEIIDEITKCVDFISL
GTNDLTQYTLAVDRNNVIVQDLFEKFHPAIIRQLHRVIATAQKNHCRVALCGDMGSDPLALPFLIGCGLRKFSVVSADIA
NLKRFVSRYSVAETEALALECIKLDSAQKIKACLETFQAEH

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=567, Percent_Identity=31.7460317460317, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1789193, Length=569, Percent_Identity=33.0404217926186, Blast_Score=289, Evalue=4e-79,
Organism=Escherichia coli, GI48994992, Length=399, Percent_Identity=33.3333333333333, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1788726, Length=507, Percent_Identity=31.3609467455621, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1787994, Length=399, Percent_Identity=24.3107769423559, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 67189; Mature: 67058

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQKKNTLKGVLPSKKQSRSNRSDGGNIKPHEATFNGIGTSRGIAIGECYAYEKEQYEH
CCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHH
EITSLDNDNIEAEIERFIEAVHRSEKELKKIERVTTRKLGKGYANIFQAQIMMLQDPMLI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHH
DSISLRIRSEMIPAHLVIEEEFGHYHEKFKSSDDVVFQERADDFFDIKNRILRNLHVRKM
HHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHH
LSWIPEGTIVVSDHFCPGDIILLSRSNVKGFLSDSGGKTSHISLICKSLNIPMVAGLGNF
HHHCCCCEEEEECCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCEEECCCCC
SQNAVSGTQMIIDGSSGTVIIHPEEKTIQLYQEKQKAEKQSNADASLTATLPASTKCGVR
CCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEE
INFFSNIDFKEELLGLKETGSEGIGLFRSENLFIDGTKVPGESEQYHYYREMSETVAPQP
EEEECCCCHHHHHCCHHHCCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCC
FVVRLFDIGGDKLIYSPIREPNPNLGWRGIRILIDVPEILENQLRAVIRANALGNINILI
EEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCEEEH
PMITSLDEIMTICAMVDRLHAEVIEQTGVAGEKPELGAMIEVPAAVEIIDEITKCVDFIS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHH
LGTNDLTQYTLAVDRNNVIVQDLFEKFHPAIIRQLHRVIATAQKNHCRVALCGDMGSDPL
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
ALPFLIGCGLRKFSVVSADIANLKRFVSRYSVAETEALALECIKLDSAQKIKACLETFQA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
EH
CC
>Mature Secondary Structure 
PEQKKNTLKGVLPSKKQSRSNRSDGGNIKPHEATFNGIGTSRGIAIGECYAYEKEQYEH
CCCHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHH
EITSLDNDNIEAEIERFIEAVHRSEKELKKIERVTTRKLGKGYANIFQAQIMMLQDPMLI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHH
DSISLRIRSEMIPAHLVIEEEFGHYHEKFKSSDDVVFQERADDFFDIKNRILRNLHVRKM
HHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHH
LSWIPEGTIVVSDHFCPGDIILLSRSNVKGFLSDSGGKTSHISLICKSLNIPMVAGLGNF
HHHCCCCEEEEECCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCEEECCCCC
SQNAVSGTQMIIDGSSGTVIIHPEEKTIQLYQEKQKAEKQSNADASLTATLPASTKCGVR
CCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEE
INFFSNIDFKEELLGLKETGSEGIGLFRSENLFIDGTKVPGESEQYHYYREMSETVAPQP
EEEECCCCHHHHHCCHHHCCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCC
FVVRLFDIGGDKLIYSPIREPNPNLGWRGIRILIDVPEILENQLRAVIRANALGNINILI
EEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCEEEH
PMITSLDEIMTICAMVDRLHAEVIEQTGVAGEKPELGAMIEVPAAVEIIDEITKCVDFIS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHH
LGTNDLTQYTLAVDRNNVIVQDLFEKFHPAIIRQLHRVIATAQKNHCRVALCGDMGSDPL
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
ALPFLIGCGLRKFSVVSADIANLKRFVSRYSVAETEALALECIKLDSAQKIKACLETFQA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
EH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7686067; 9384377; 10568751; 2497294; 8449881; 1495386 [H]