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The map label for this gene is birA [H]

Identifier: 119355961

GI number: 119355961

Start: 131086

End: 132072

Strand: Direct

Name: birA [H]

Synonym: Cpha266_0111

Alternate gene names: 119355961

Gene position: 131086-132072 (Clockwise)

Preceding gene: 119355960

Following gene: 119355962

Centisome position: 4.18

GC content: 53.09

Gene sequence:

>987_bases
ATGAACCAGATTTCCTCAAGCATTCTGCATCATCTTCGCCTGTCCGGCGGTTTTCTTTCCGGCGAGGATATCTCCGTTTC
TCTGGGTATTACGCGAAGCGCGGTGTGGAAACATATCGGAGCGTTACGGCGCGAGGGATATCGTATTGATGCGGTAAGCG
GGCAGGGTTACAGATTGTTTTCGTTGAGCGGGCGTCCCGTTCATGAAGAGGTTTCGCCCTGGCTTACGACCCGGTTTATC
GGCAGAGATCTTCATTATCATGAACTTCTTGAGTCTACCAATTTTTCGGCACGAGTGCTTGCCGGAACGGGGACGCGGGA
GGGATCGGTTGTTGTTGCCGATGCGCAGTCCGGAGGGCGAGGGAGAATGATGCGCAGTTGGGTTTCTCCCGGAGGCGTCA
ATCTTTATTTTTCGCTGATTCTTCGACCAAGGGTTCCGGGCATCCGGGTTCCTCAGCTTACCCTGCTTGCGGCTGCGGCT
GTTCATCAGGCGCTTTGTTCCTGCGTTCCTGAAATCGCATCGCGGATCAAATGGCCGAACGATATCATGGTCGGGGAGAA
AAAACTGTGCGGCGTGCTTTGTGAAATGCAGTCAGAGCCGGATATGACCCATTTTGTTATTATCGGTATCGGTATCAATG
TCAATATCGGCGAGTTTCCCGATGAACTGCGGAACATCGCCACCTCTCTTTTTCTTGAGTCCGGGCGCATGGTTTCACGC
CCCGAGCTGCTTGCCTCGGTGCTTAACCACTTCGAGTCGCTTTATGAGGCGTGGCTGCTTCATGACGATCTTGGTTTCGT
GCTCCCCTATCTCCATGAATATTCATTGGTTTATCGTCGGCAGGTGATTATTCGCCAGACGACAGGCGATGTGTGCGGTA
CCGTTTCCGGGATTGCCCGGGGAGGGGAACTGATGCTTGACCTTGACGATGCCAGAACAGTTTTGATCTCATCCGGTGAT
TGTCTTCTTTGTCGTCAATCGCCCTGA

Upstream 100 bases:

>100_bases
GGTCGCTGTTTTTTTTTGTTGCGTGATTGTCAACCTCGCGCTACATTCAGGTTTACAATTGTTGGCCGAAGTTTCCGTTT
CGATTTGTTTTTCGCAGCAC

Downstream 100 bases:

>100_bases
CCCTCTCATAGTATGACACGTATGCGTCCCGTTCCGCTCCATCCCGATATTTTGCAGGCTTACGCAGTTCTTGAAACCGG
AGAGCCTGTTTGCAGGGAAC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNQISSSILHHLRLSGGFLSGEDISVSLGITRSAVWKHIGALRREGYRIDAVSGQGYRLFSLSGRPVHEEVSPWLTTRFI
GRDLHYHELLESTNFSARVLAGTGTREGSVVVADAQSGGRGRMMRSWVSPGGVNLYFSLILRPRVPGIRVPQLTLLAAAA
VHQALCSCVPEIASRIKWPNDIMVGEKKLCGVLCEMQSEPDMTHFVIIGIGINVNIGEFPDELRNIATSLFLESGRMVSR
PELLASVLNHFESLYEAWLLHDDLGFVLPYLHEYSLVYRRQVIIRQTTGDVCGTVSGIARGGELMLDLDDARTVLISSGD
CLLCRQSP

Sequences:

>Translated_328_residues
MNQISSSILHHLRLSGGFLSGEDISVSLGITRSAVWKHIGALRREGYRIDAVSGQGYRLFSLSGRPVHEEVSPWLTTRFI
GRDLHYHELLESTNFSARVLAGTGTREGSVVVADAQSGGRGRMMRSWVSPGGVNLYFSLILRPRVPGIRVPQLTLLAAAA
VHQALCSCVPEIASRIKWPNDIMVGEKKLCGVLCEMQSEPDMTHFVIIGIGINVNIGEFPDELRNIATSLFLESGRMVSR
PELLASVLNHFESLYEAWLLHDDLGFVLPYLHEYSLVYRRQVIIRQTTGDVCGTVSGIARGGELMLDLDDARTVLISSGD
CLLCRQSP
>Mature_328_residues
MNQISSSILHHLRLSGGFLSGEDISVSLGITRSAVWKHIGALRREGYRIDAVSGQGYRLFSLSGRPVHEEVSPWLTTRFI
GRDLHYHELLESTNFSARVLAGTGTREGSVVVADAQSGGRGRMMRSWVSPGGVNLYFSLILRPRVPGIRVPQLTLLAAAA
VHQALCSCVPEIASRIKWPNDIMVGEKKLCGVLCEMQSEPDMTHFVIIGIGINVNIGEFPDELRNIATSLFLESGRMVSR
PELLASVLNHFESLYEAWLLHDDLGFVLPYLHEYSLVYRRQVIIRQTTGDVCGTVSGIARGGELMLDLDDARTVLISSGD
CLLCRQSP

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=299, Percent_Identity=28.0936454849498, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36141; Mature: 36141

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQISSSILHHLRLSGGFLSGEDISVSLGITRSAVWKHIGALRREGYRIDAVSGQGYRLF
CCHHHHHHHHHHHHCCCEECCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEECCCCEEEE
SLSGRPVHEEVSPWLTTRFIGRDLHYHELLESTNFSARVLAGTGTREGSVVVADAQSGGR
EECCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCC
GRMMRSWVSPGGVNLYFSLILRPRVPGIRVPQLTLLAAAAVHQALCSCVPEIASRIKWPN
CHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DIMVGEKKLCGVLCEMQSEPDMTHFVIIGIGINVNIGEFPDELRNIATSLFLESGRMVSR
CEECCCHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PELLASVLNHFESLYEAWLLHDDLGFVLPYLHEYSLVYRRQVIIRQTTGDVCGTVSGIAR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCC
GGELMLDLDDARTVLISSGDCLLCRQSP
CCEEEEEECCCEEEEEECCCEEEEECCC
>Mature Secondary Structure
MNQISSSILHHLRLSGGFLSGEDISVSLGITRSAVWKHIGALRREGYRIDAVSGQGYRLF
CCHHHHHHHHHHHHCCCEECCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEECCCCEEEE
SLSGRPVHEEVSPWLTTRFIGRDLHYHELLESTNFSARVLAGTGTREGSVVVADAQSGGR
EECCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCC
GRMMRSWVSPGGVNLYFSLILRPRVPGIRVPQLTLLAAAAVHQALCSCVPEIASRIKWPN
CHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DIMVGEKKLCGVLCEMQSEPDMTHFVIIGIGINVNIGEFPDELRNIATSLFLESGRMVSR
CEECCCHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PELLASVLNHFESLYEAWLLHDDLGFVLPYLHEYSLVYRRQVIIRQTTGDVCGTVSGIAR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCC
GGELMLDLDDARTVLISSGDCLLCRQSP
CCEEEEEECCCEEEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]