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The map label for this gene is amiA [H]

Identifier: 119355960

GI number: 119355960

Start: 129197

End: 130975

Strand: Direct

Name: amiA [H]

Synonym: Cpha266_0110

Alternate gene names: 119355960

Gene position: 129197-130975 (Clockwise)

Preceding gene: 119355958

Following gene: 119355961

Centisome position: 4.12

GC content: 50.93

Gene sequence:

>1779_bases
ATGGTTACCGGTTTTGTAATACGGCAATACTTTTTATTCATCATCATGAGTTCATTCGGTTTCCGATTGTTTCGGTTGTT
TTTCTGCCAGCTCTGCCTGGTTCTCGCACTGGTATCGATGGCGTTACTCGATGCCGAGGGCGTTGTCCGGCCTGAATCGG
CTACGCTGTCTCTGCAGGTAAGCAAAGGCAACGGAGAGGGATACAGGATAAAGGTTTTTGCGTTAAGAAAAGCGGGAAAT
ATTCTCATTGATGTTGAGTCTTTTGCAAGGGCTTTGCGTCTCGGCTTCCGGCAGAGTAACGGTTTTCTTGTCATTGACGA
GGCTGAAACGCTCTCCGGAAGCAGTTGTCAGCTAAGAGACGGAAATAATTTTGTCTCCATAGTTTCGAAAGATCCCCGTC
AGGAGCCGGGCACCATGCAGTTACAGGCAACTCCGGTTCAGATCGCATCGAAACTCTATCTTCCTGTTTCCCAGGCATGC
AGGTTTTTTTCCCTGTGGCTTCAACGGGAGGTTCGTTATGATCCGGCAACCGGGCTTATAAGCGCCATATTATGGGGAGA
GAGGCCTGCGTATTCGCTGAAAAAATTCAGCTCGTCGATTCAGGACGAGCCGGAGCCTGTCGGCAGTAACGGTAAGCGCG
CAGCGTCTTCCGAAGCGCTTACCGTTATCAGCGGCGTCAGCGTTGAAAATCGGGAAAACGGCGCGCTCATAAGTTTTTCG
GCGGTGGGTAACAGCGTCAGGACGCTGTTGATGAAGCCGGACAAGAACGGTATCGCATACCTCACGTTCCAGAACGCAAC
CGGCAATGTTGACGAAATGACAAAAAGTTTCGGCGTCGGTATTGTTCAAGCTGTTAAGGCGAAGCGTTTTCAAAAAGGCG
GATTTCAGGTGGCGCTTTCGATGGATAACCGCTCTTACCGGATCAATTCGATGGAGGTGAAGCGCGACGATATCAATAAC
AGGTATCTCGTGTATGTCGCTGGCGAGCCTGATACCGGAGGGGGCGGGATGAGAGAGAAGGAGAGTCAGATTTCACGGGT
TATTGAGAGAGACATCGAGAAGTGGAAGTTTGATACCGTCGTGCTCGATGCCGGTCACGGAGGAAAGGATCCCGGAGCAA
TCGGCGGTCACGGTACGATGGAAAAAGATGTCGTTCTGAATATTGTGCGTGATCTTGGTTCCATCATTGCGCGCCAGTGG
CCCGATATCAAGGTTGTCTATACCAGAAACTCCGATGTTTTTATTCCTCTGCATGAACGGGGAAAAATCGCTAACCGGAG
CGGCGGCAAGCTTTTTGTGAGCATTCATTGCAACGCAAACAGCAAAAGCCATATCAGGGGGCAGGAGGTCTATATTCTTG
GCCCGCACAGGACTCAGAAATCGCTTGAGGTTGCCATGTTTGAAAATTCGGTTATTACCAGAGAGGCTGATTACCGGGAG
CGCTACAAAGGGTTTTCGACAGAGTATCTGATCATGAGCAGCATGGCGCAGAATGCTTTTGCAAAACAGTCGACCACGCT
TGCGCAGGATGTTCTGAAACGGATCGAGAGAAAGGGGGGGACTACCAGCAGGGGCGTGCATCAGGCGGGTTTTATGGTGC
TCTGGACACCCTCAATGCCGAGTATCCTGATCGAGACCGGCTATCTTTCCAATCCCGAGGAAGAACGGATACTCAGAGAA
CGGAAGGAGCAGATCAGAATCGCCGAAGCCGTATTTCAGGGGCTGGATCGGTATCGCAGAAATAACGAAACAGCACGGCT
TTCGGCAGTTGAGCGTTGA

Upstream 100 bases:

>100_bases
AAAAAACAAATTTTCGGCGATTTCCGGTACGGCGATCTTTTTGCTGAAAAACCAGGCTTATCAGGTTTATGTGAACAATA
TATTTTATTCTTTAATTTAC

Downstream 100 bases:

>100_bases
CGGGCGACGCGGTCGCTGTTTTTTTTTGTTGCGTGATTGTCAACCTCGCGCTACATTCAGGTTTACAATTGTTGGCCGAA
GTTTCCGTTTCGATTTGTTT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MVTGFVIRQYFLFIIMSSFGFRLFRLFFCQLCLVLALVSMALLDAEGVVRPESATLSLQVSKGNGEGYRIKVFALRKAGN
ILIDVESFARALRLGFRQSNGFLVIDEAETLSGSSCQLRDGNNFVSIVSKDPRQEPGTMQLQATPVQIASKLYLPVSQAC
RFFSLWLQREVRYDPATGLISAILWGERPAYSLKKFSSSIQDEPEPVGSNGKRAASSEALTVISGVSVENRENGALISFS
AVGNSVRTLLMKPDKNGIAYLTFQNATGNVDEMTKSFGVGIVQAVKAKRFQKGGFQVALSMDNRSYRINSMEVKRDDINN
RYLVYVAGEPDTGGGGMREKESQISRVIERDIEKWKFDTVVLDAGHGGKDPGAIGGHGTMEKDVVLNIVRDLGSIIARQW
PDIKVVYTRNSDVFIPLHERGKIANRSGGKLFVSIHCNANSKSHIRGQEVYILGPHRTQKSLEVAMFENSVITREADYRE
RYKGFSTEYLIMSSMAQNAFAKQSTTLAQDVLKRIERKGGTTSRGVHQAGFMVLWTPSMPSILIETGYLSNPEEERILRE
RKEQIRIAEAVFQGLDRYRRNNETARLSAVER

Sequences:

>Translated_592_residues
MVTGFVIRQYFLFIIMSSFGFRLFRLFFCQLCLVLALVSMALLDAEGVVRPESATLSLQVSKGNGEGYRIKVFALRKAGN
ILIDVESFARALRLGFRQSNGFLVIDEAETLSGSSCQLRDGNNFVSIVSKDPRQEPGTMQLQATPVQIASKLYLPVSQAC
RFFSLWLQREVRYDPATGLISAILWGERPAYSLKKFSSSIQDEPEPVGSNGKRAASSEALTVISGVSVENRENGALISFS
AVGNSVRTLLMKPDKNGIAYLTFQNATGNVDEMTKSFGVGIVQAVKAKRFQKGGFQVALSMDNRSYRINSMEVKRDDINN
RYLVYVAGEPDTGGGGMREKESQISRVIERDIEKWKFDTVVLDAGHGGKDPGAIGGHGTMEKDVVLNIVRDLGSIIARQW
PDIKVVYTRNSDVFIPLHERGKIANRSGGKLFVSIHCNANSKSHIRGQEVYILGPHRTQKSLEVAMFENSVITREADYRE
RYKGFSTEYLIMSSMAQNAFAKQSTTLAQDVLKRIERKGGTTSRGVHQAGFMVLWTPSMPSILIETGYLSNPEEERILRE
RKEQIRIAEAVFQGLDRYRRNNETARLSAVER
>Mature_592_residues
MVTGFVIRQYFLFIIMSSFGFRLFRLFFCQLCLVLALVSMALLDAEGVVRPESATLSLQVSKGNGEGYRIKVFALRKAGN
ILIDVESFARALRLGFRQSNGFLVIDEAETLSGSSCQLRDGNNFVSIVSKDPRQEPGTMQLQATPVQIASKLYLPVSQAC
RFFSLWLQREVRYDPATGLISAILWGERPAYSLKKFSSSIQDEPEPVGSNGKRAASSEALTVISGVSVENRENGALISFS
AVGNSVRTLLMKPDKNGIAYLTFQNATGNVDEMTKSFGVGIVQAVKAKRFQKGGFQVALSMDNRSYRINSMEVKRDDINN
RYLVYVAGEPDTGGGGMREKESQISRVIERDIEKWKFDTVVLDAGHGGKDPGAIGGHGTMEKDVVLNIVRDLGSIIARQW
PDIKVVYTRNSDVFIPLHERGKIANRSGGKLFVSIHCNANSKSHIRGQEVYILGPHRTQKSLEVAMFENSVITREADYRE
RYKGFSTEYLIMSSMAQNAFAKQSTTLAQDVLKRIERKGGTTSRGVHQAGFMVLWTPSMPSILIETGYLSNPEEERILRE
RKEQIRIAEAVFQGLDRYRRNNETARLSAVER

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=222, Percent_Identity=36.4864864864865, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI87082163, Length=223, Percent_Identity=36.322869955157, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=32.4444444444444, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 65969; Mature: 65969

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTGFVIRQYFLFIIMSSFGFRLFRLFFCQLCLVLALVSMALLDAEGVVRPESATLSLQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
SKGNGEGYRIKVFALRKAGNILIDVESFARALRLGFRQSNGFLVIDEAETLSGSSCQLRD
ECCCCCCEEEEEEEEECCCCEEEEHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEC
GNNFVSIVSKDPRQEPGTMQLQATPVQIASKLYLPVSQACRFFSLWLQREVRYDPATGLI
CCCEEEEEECCCCCCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHH
SAILWGERPAYSLKKFSSSIQDEPEPVGSNGKRAASSEALTVISGVSVENRENGALISFS
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEH
AVGNSVRTLLMKPDKNGIAYLTFQNATGNVDEMTKSFGVGIVQAVKAKRFQKGGFQVALS
HCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEE
MDNRSYRINSMEVKRDDINNRYLVYVAGEPDTGGGGMREKESQISRVIERDIEKWKFDTV
ECCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEE
VLDAGHGGKDPGAIGGHGTMEKDVVLNIVRDLGSIIARQWPDIKVVYTRNSDVFIPLHER
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCC
GKIANRSGGKLFVSIHCNANSKSHIRGQEVYILGPHRTQKSLEVAMFENSVITREADYRE
CCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCHHHH
RYKGFSTEYLIMSSMAQNAFAKQSTTLAQDVLKRIERKGGTTSRGVHQAGFMVLWTPSMP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEECCCCC
SILIETGYLSNPEEERILRERKEQIRIAEAVFQGLDRYRRNNETARLSAVER
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure
MVTGFVIRQYFLFIIMSSFGFRLFRLFFCQLCLVLALVSMALLDAEGVVRPESATLSLQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
SKGNGEGYRIKVFALRKAGNILIDVESFARALRLGFRQSNGFLVIDEAETLSGSSCQLRD
ECCCCCCEEEEEEEEECCCCEEEEHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEC
GNNFVSIVSKDPRQEPGTMQLQATPVQIASKLYLPVSQACRFFSLWLQREVRYDPATGLI
CCCEEEEEECCCCCCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHH
SAILWGERPAYSLKKFSSSIQDEPEPVGSNGKRAASSEALTVISGVSVENRENGALISFS
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEH
AVGNSVRTLLMKPDKNGIAYLTFQNATGNVDEMTKSFGVGIVQAVKAKRFQKGGFQVALS
HCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEE
MDNRSYRINSMEVKRDDINNRYLVYVAGEPDTGGGGMREKESQISRVIERDIEKWKFDTV
ECCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEE
VLDAGHGGKDPGAIGGHGTMEKDVVLNIVRDLGSIIARQWPDIKVVYTRNSDVFIPLHER
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCC
GKIANRSGGKLFVSIHCNANSKSHIRGQEVYILGPHRTQKSLEVAMFENSVITREADYRE
CCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCHHHH
RYKGFSTEYLIMSSMAQNAFAKQSTTLAQDVLKRIERKGGTTSRGVHQAGFMVLWTPSMP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEECCCCC
SILIETGYLSNPEEERILRERKEQIRIAEAVFQGLDRYRRNNETARLSAVER
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]