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The map label for this gene is bioB [H]

Identifier: 119355962

GI number: 119355962

Start: 132085

End: 133098

Strand: Direct

Name: bioB [H]

Synonym: Cpha266_0112

Alternate gene names: 119355962

Gene position: 132085-133098 (Clockwise)

Preceding gene: 119355961

Following gene: 119355963

Centisome position: 4.21

GC content: 52.37

Gene sequence:

>1014_bases
ATGACACGTATGCGTCCCGTTCCGCTCCATCCCGATATTTTGCAGGCTTACGCAGTTCTTGAAACCGGAGAGCCTGTTTG
CAGGGAACTTGCTTTTGCGTTGGGCGAATTGCATGGCCCGGATGTGCTTGATCTCGCCTCTCTTGCCCATAAAGTAAAAC
TCAGGCATGGCGGCTCTTCAGGATCGATTCATGCCTGTTCGATCATGAATGCCAGGTCGGGAGTGTGTTCTGAAAACTGC
CGGTTCTGTGCGCAGTCGGCCCATCACCAAGCGGCAATTGACGTTTACGGACTTGTGGATGTTGACGCCGTGCTTTTTCA
TGCGCGGCAAACAGCCAGTGAAGGCATATCGCATTTCGGCATTGTGACCAGCGGATTTGGTTATAAAACGTTGTCGAAAG
AGTTTCGGCAGATACTTGCCATGATCGACCGTCTGCATCAGGAACTTCCCGATCTTGAAATCTGCGCATCTCTCGGAGTT
CTTGGCGAGGAGCCTGCTTTGGAACTTGCCCGTCACGGCATCGCACAGTACAATATCAATATCCAGGTCGCCCCTCGTCG
GTATGGCGAACTCATCGCCGACACCCATAGCGTCGATGACCGGATTGATACGATTAAACGGCTGCGACGTAACAATATAG
ATGTTTGCTGCGGCGGCATTATCGGCGTTGGCGAGCAGATGAAGGAGAGAGTAGAGATGATATTCGCGTTTGCCGATCTC
GATGTTTCGGTGATTCCCCTCAACATATTGGTACCGATTGACGGAACCCCTCTTGAAGGTTCGCCAGGCATTCCGCTCGA
CGACATTGTTAAAACCTTTGCTCTCTGTCGTCTTGTTCATCCTCGGAAAATCATCAAGATTGCCGCAGGACGGGAAACGG
TCATGAAGGATTTTCAGGGGCTTTTGATGCTTTCGGGTGCGGACGGGCTGCTTACCGGCGGCTATCTTACCACAAGGGGG
CGAGCGACAGCGGATGACCGCATGTTCATGCGGCAACTCCAATGGTTCAACTGA

Upstream 100 bases:

>100_bases
GGGAGGGGAACTGATGCTTGACCTTGACGATGCCAGAACAGTTTTGATCTCATCCGGTGATTGTCTTCTTTGTCGTCAAT
CGCCCTGACCCTCTCATAGT

Downstream 100 bases:

>100_bases
GCGGAAGCATGATGCCGATCCGTGAATCAATAGAGCGGGAGCTGCTGGCGCTTCGCCAGAAGGGCCGCTACCGGGAACTT
CCTTCCGTAACGCAGCGGAG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTRMRPVPLHPDILQAYAVLETGEPVCRELAFALGELHGPDVLDLASLAHKVKLRHGGSSGSIHACSIMNARSGVCSENC
RFCAQSAHHQAAIDVYGLVDVDAVLFHARQTASEGISHFGIVTSGFGYKTLSKEFRQILAMIDRLHQELPDLEICASLGV
LGEEPALELARHGIAQYNINIQVAPRRYGELIADTHSVDDRIDTIKRLRRNNIDVCCGGIIGVGEQMKERVEMIFAFADL
DVSVIPLNILVPIDGTPLEGSPGIPLDDIVKTFALCRLVHPRKIIKIAAGRETVMKDFQGLLMLSGADGLLTGGYLTTRG
RATADDRMFMRQLQWFN

Sequences:

>Translated_337_residues
MTRMRPVPLHPDILQAYAVLETGEPVCRELAFALGELHGPDVLDLASLAHKVKLRHGGSSGSIHACSIMNARSGVCSENC
RFCAQSAHHQAAIDVYGLVDVDAVLFHARQTASEGISHFGIVTSGFGYKTLSKEFRQILAMIDRLHQELPDLEICASLGV
LGEEPALELARHGIAQYNINIQVAPRRYGELIADTHSVDDRIDTIKRLRRNNIDVCCGGIIGVGEQMKERVEMIFAFADL
DVSVIPLNILVPIDGTPLEGSPGIPLDDIVKTFALCRLVHPRKIIKIAAGRETVMKDFQGLLMLSGADGLLTGGYLTTRG
RATADDRMFMRQLQWFN
>Mature_336_residues
TRMRPVPLHPDILQAYAVLETGEPVCRELAFALGELHGPDVLDLASLAHKVKLRHGGSSGSIHACSIMNARSGVCSENCR
FCAQSAHHQAAIDVYGLVDVDAVLFHARQTASEGISHFGIVTSGFGYKTLSKEFRQILAMIDRLHQELPDLEICASLGVL
GEEPALELARHGIAQYNINIQVAPRRYGELIADTHSVDDRIDTIKRLRRNNIDVCCGGIIGVGEQMKERVEMIFAFADLD
VSVIPLNILVPIDGTPLEGSPGIPLDDIVKTFALCRLVHPRKIIKIAAGRETVMKDFQGLLMLSGADGLLTGGYLTTRGR
ATADDRMFMRQLQWFN

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=303, Percent_Identity=29.3729372937294, Blast_Score=156, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321725, Length=308, Percent_Identity=29.2207792207792, Blast_Score=159, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36931; Mature: 36800

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRMRPVPLHPDILQAYAVLETGEPVCRELAFALGELHGPDVLDLASLAHKVKLRHGGSS
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
GSIHACSIMNARSGVCSENCRFCAQSAHHQAAIDVYGLVDVDAVLFHARQTASEGISHFG
CCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHEEEEEHHHHHHHHHHHHHHHHHCHHHHC
IVTSGFGYKTLSKEFRQILAMIDRLHQELPDLEICASLGVLGEEPALELARHGIAQYNIN
CEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCEEEEEE
IQVAPRRYGELIADTHSVDDRIDTIKRLRRNNIDVCCGGIIGVGEQMKERVEMIFAFADL
EEECCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECHHHCCCHHHHHHHHHHHHHHCC
DVSVIPLNILVPIDGTPLEGSPGIPLDDIVKTFALCRLVHPRKIIKIAAGRETVMKDFQG
CEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHC
LLMLSGADGLLTGGYLTTRGRATADDRMFMRQLQWFN
EEEEECCCCCEECCEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRMRPVPLHPDILQAYAVLETGEPVCRELAFALGELHGPDVLDLASLAHKVKLRHGGSS
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
GSIHACSIMNARSGVCSENCRFCAQSAHHQAAIDVYGLVDVDAVLFHARQTASEGISHFG
CCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHEEEEEHHHHHHHHHHHHHHHHHCHHHHC
IVTSGFGYKTLSKEFRQILAMIDRLHQELPDLEICASLGVLGEEPALELARHGIAQYNIN
CEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCEEEEEE
IQVAPRRYGELIADTHSVDDRIDTIKRLRRNNIDVCCGGIIGVGEQMKERVEMIFAFADL
EEECCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECHHHCCCHHHHHHHHHHHHHHCC
DVSVIPLNILVPIDGTPLEGSPGIPLDDIVKTFALCRLVHPRKIIKIAAGRETVMKDFQG
CEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHC
LLMLSGADGLLTGGYLTTRGRATADDRMFMRQLQWFN
EEEEECCCCCEECCEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA