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The map label for this gene is emrB [H]

Identifier: 119355943

GI number: 119355943

Start: 107423

End: 108994

Strand: Reverse

Name: emrB [H]

Synonym: Cpha266_0093

Alternate gene names: 119355943

Gene position: 108994-107423 (Counterclockwise)

Preceding gene: 119355944

Following gene: 119355942

Centisome position: 3.48

GC content: 56.74

Gene sequence:

>1572_bases
ATGCTGCCGGCGGCACATGCCTATGAAACAGGCTCGCGAAGGATCATCATCACCCTGACCGTGATTGTCTCCGCGATGCT
TGAACTGATCGACACCACCATTGTCAACGTAGCCATCACAGAAATCAGCGGAAACCTCGGAGCAAGCATCGAGGATGTCG
CCTGGGTGGTCACCAGCTACGCCATCGCAAACGTCATCGTTATTCCGCTTTCGGGGTTTCTCGGCAACCTGCTCGGACGC
CGGAACTACTACATCGGCTCGATTCTGCTCTTCACGGCGGCATCGCTGCTCTGCGGCATCGCAACCGACATCTGGACGCT
GGTCTTTTTCCGTTTTCTGCAAGGAATCGGAGGCGGAGCGCTGCTGCCGACCTCCCAGGCAATCCTTTACGAAACCTATC
CACCCGAAGACCGGGGGAAAGCAACCGGTATTTTTTCCATGGGCCTCGTGCTCGGACCTACTTTCGGTCCGCTGCTCGGC
GGATTTCTGGTTGACAACTTCAGCTGGGAGTGGTGTTTTTTCGTAAACATACCGGTAGGCCTGCTTGCAGCATGGTCAGC
GTTCACCTTCATCAAGGAGCCGAAAACCGTTCATAAGGTTGTAAAAATCGACTGGGCCGGCATCGGCCTTCTCACGATTG
GTATCGGCTCGCTGCAGTTCATCCTCGAACGCGGCGAATCGAAAGACTGGTTCGAAACCCCTTACATCACCTGGTTCACC
ATCATAGCCATCGTCTCGCTTATGCTCTTTGTCTGGTGGGAGCTGCGTGTAGAGGAACCTGCCGTTGACCTCAGCGTGCT
TGCCCGAAGCCATAATCTTGCCATTGCCGCCGTCCTGACCTTTGTGGTTGGTTATGGACTCTACGGATCGCTCTTTGTCT
TTCCGGTCTTCGTCCAGCGCCTGCTCGGCTTCTCGGCGCTTCTCACCGGCACGGTGCTCTTTCCAAGTGCGCTTGTCACA
GGAATCATCGCGCTTCCTCTCGGGATCGCGCTGCAAAAAGGGGCCTCGGCGAAAATTCTCATGACCGTCGGCATGGCGGC
ATTTTCGATCTTCTGCTGGGAACTTGGCCAGCAGACCATGCAGTCGGGAGCCGGAAACTTTTTCTGGATACTGCTCCTGC
GCGGCGTCGCCCTCGGATTCATCTTCATACCCGTTACCATGCTTGCCGTTGCCGGACTGCACGGCAAGGATATCGGGCAG
GCAACCGGCCTGAACAACATGGTACGACAGCTCGGAGGATCGTTCGGCATTGCGCTAACCAACACCTATATCGTCAAGCG
GGTCGCCGAACACCGTAACGAAATCCTCAGCAACCTCTCGCCCTACGACCCCGCGGCCGTCGAACGCCTCAACGCCATAG
GGCAAGCCGCACAGTCGCGCATGATCTCTCCGGTTGAAGCAGAGCAGGTCGCACTGCGTGCGCTCGAAGGAACGGTCACC
GCACAGAGCTACCATCTTGCCTACATGGACGCCTTCATGCTGATCGCCCTGCTCTTTGCGCTCTGCATGCCGCTGCTCTT
CTTCATCAGAATTAAAAAAGGGGAAAAAGCCGACCTCTCACAGGCGCACTGA

Upstream 100 bases:

>100_bases
TCGAAATGACTAATTACAAACATACAGGAATGTGTCAACAGCATAGCGAGCAACATTAATTATGGCACAACAACAAAACG
GCGTTACCGCCGTAGCGGCA

Downstream 100 bases:

>100_bases
GACAGATTTTCTTTTGCATATATCGCCAACGATACCTATCTTTGGTATATATCAATCCGAATGTATCGGGGTGATCCGAA
CGACAAAGAAAGAATTTCAA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MLPAAHAYETGSRRIIITLTVIVSAMLELIDTTIVNVAITEISGNLGASIEDVAWVVTSYAIANVIVIPLSGFLGNLLGR
RNYYIGSILLFTAASLLCGIATDIWTLVFFRFLQGIGGGALLPTSQAILYETYPPEDRGKATGIFSMGLVLGPTFGPLLG
GFLVDNFSWEWCFFVNIPVGLLAAWSAFTFIKEPKTVHKVVKIDWAGIGLLTIGIGSLQFILERGESKDWFETPYITWFT
IIAIVSLMLFVWWELRVEEPAVDLSVLARSHNLAIAAVLTFVVGYGLYGSLFVFPVFVQRLLGFSALLTGTVLFPSALVT
GIIALPLGIALQKGASAKILMTVGMAAFSIFCWELGQQTMQSGAGNFFWILLLRGVALGFIFIPVTMLAVAGLHGKDIGQ
ATGLNNMVRQLGGSFGIALTNTYIVKRVAEHRNEILSNLSPYDPAAVERLNAIGQAAQSRMISPVEAEQVALRALEGTVT
AQSYHLAYMDAFMLIALLFALCMPLLFFIRIKKGEKADLSQAH

Sequences:

>Translated_523_residues
MLPAAHAYETGSRRIIITLTVIVSAMLELIDTTIVNVAITEISGNLGASIEDVAWVVTSYAIANVIVIPLSGFLGNLLGR
RNYYIGSILLFTAASLLCGIATDIWTLVFFRFLQGIGGGALLPTSQAILYETYPPEDRGKATGIFSMGLVLGPTFGPLLG
GFLVDNFSWEWCFFVNIPVGLLAAWSAFTFIKEPKTVHKVVKIDWAGIGLLTIGIGSLQFILERGESKDWFETPYITWFT
IIAIVSLMLFVWWELRVEEPAVDLSVLARSHNLAIAAVLTFVVGYGLYGSLFVFPVFVQRLLGFSALLTGTVLFPSALVT
GIIALPLGIALQKGASAKILMTVGMAAFSIFCWELGQQTMQSGAGNFFWILLLRGVALGFIFIPVTMLAVAGLHGKDIGQ
ATGLNNMVRQLGGSFGIALTNTYIVKRVAEHRNEILSNLSPYDPAAVERLNAIGQAAQSRMISPVEAEQVALRALEGTVT
AQSYHLAYMDAFMLIALLFALCMPLLFFIRIKKGEKADLSQAH
>Mature_523_residues
MLPAAHAYETGSRRIIITLTVIVSAMLELIDTTIVNVAITEISGNLGASIEDVAWVVTSYAIANVIVIPLSGFLGNLLGR
RNYYIGSILLFTAASLLCGIATDIWTLVFFRFLQGIGGGALLPTSQAILYETYPPEDRGKATGIFSMGLVLGPTFGPLLG
GFLVDNFSWEWCFFVNIPVGLLAAWSAFTFIKEPKTVHKVVKIDWAGIGLLTIGIGSLQFILERGESKDWFETPYITWFT
IIAIVSLMLFVWWELRVEEPAVDLSVLARSHNLAIAAVLTFVVGYGLYGSLFVFPVFVQRLLGFSALLTGTVLFPSALVT
GIIALPLGIALQKGASAKILMTVGMAAFSIFCWELGQQTMQSGAGNFFWILLLRGVALGFIFIPVTMLAVAGLHGKDIGQ
ATGLNNMVRQLGGSFGIALTNTYIVKRVAEHRNEILSNLSPYDPAAVERLNAIGQAAQSRMISPVEAEQVALRALEGTVT
AQSYHLAYMDAFMLIALLFALCMPLLFFIRIKKGEKADLSQAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=503, Percent_Identity=31.8091451292247, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1788710, Length=505, Percent_Identity=30.6930693069307, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1790195, Length=418, Percent_Identity=26.0765550239234, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081983, Length=404, Percent_Identity=25.4950495049505, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788392, Length=294, Percent_Identity=30.6122448979592, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87082320, Length=200, Percent_Identity=23.5, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082231, Length=160, Percent_Identity=26.25, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=482, Percent_Identity=24.896265560166, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319770, Length=382, Percent_Identity=25.3926701570681, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323735, Length=333, Percent_Identity=24.3243243243243, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=28.1045751633987, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=132, Percent_Identity=28.030303030303, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323520, Length=355, Percent_Identity=26.1971830985915, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=316, Percent_Identity=23.4177215189873, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56718; Mature: 56718

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPAAHAYETGSRRIIITLTVIVSAMLELIDTTIVNVAITEISGNLGASIEDVAWVVTSY
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
AIANVIVIPLSGFLGNLLGRRNYYIGSILLFTAASLLCGIATDIWTLVFFRFLQGIGGGA
HHHHHHEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LLPTSQAILYETYPPEDRGKATGIFSMGLVLGPTFGPLLGGFLVDNFSWEWCFFVNIPVG
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHH
LLAAWSAFTFIKEPKTVHKVVKIDWAGIGLLTIGIGSLQFILERGESKDWFETPYITWFT
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
IIAIVSLMLFVWWELRVEEPAVDLSVLARSHNLAIAAVLTFVVGYGLYGSLFVFPVFVQR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGFSALLTGTVLFPSALVTGIIALPLGIALQKGASAKILMTVGMAAFSIFCWELGQQTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QSGAGNFFWILLLRGVALGFIFIPVTMLAVAGLHGKDIGQATGLNNMVRQLGGSFGIALT
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHCCCCCCCHHH
NTYIVKRVAEHRNEILSNLSPYDPAAVERLNAIGQAAQSRMISPVEAEQVALRALEGTVT
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEE
AQSYHLAYMDAFMLIALLFALCMPLLFFIRIKKGEKADLSQAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLPAAHAYETGSRRIIITLTVIVSAMLELIDTTIVNVAITEISGNLGASIEDVAWVVTSY
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
AIANVIVIPLSGFLGNLLGRRNYYIGSILLFTAASLLCGIATDIWTLVFFRFLQGIGGGA
HHHHHHEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LLPTSQAILYETYPPEDRGKATGIFSMGLVLGPTFGPLLGGFLVDNFSWEWCFFVNIPVG
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHH
LLAAWSAFTFIKEPKTVHKVVKIDWAGIGLLTIGIGSLQFILERGESKDWFETPYITWFT
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
IIAIVSLMLFVWWELRVEEPAVDLSVLARSHNLAIAAVLTFVVGYGLYGSLFVFPVFVQR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGFSALLTGTVLFPSALVTGIIALPLGIALQKGASAKILMTVGMAAFSIFCWELGQQTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QSGAGNFFWILLLRGVALGFIFIPVTMLAVAGLHGKDIGQATGLNNMVRQLGGSFGIALT
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHCCCCCCCHHH
NTYIVKRVAEHRNEILSNLSPYDPAAVERLNAIGQAAQSRMISPVEAEQVALRALEGTVT
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEE
AQSYHLAYMDAFMLIALLFALCMPLLFFIRIKKGEKADLSQAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]